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14
datasets available to search
ShareScore release 0.9.0
Dataset results
14 results for “ALK5”
Determining the suitability of C2C12 and HEK293 in dual luciferase assay (DLA) for ACVR1 (ALK2) and TGFBR1 (ALK5)
<p>Aside from having high potency towards ACVR1/ALK2, inhibitor compounds ideally should be highly selective and not target other members of the Transforming Growth Factor beta (TGFb) superfamily. TGFBR1/ALK5 is selected for off-target screening because of its potential role in cardiac functions.</p>
Determining the dual luciferase ALK5 IC50 values of 30 legacy ACVR1/ALK2 inhibitors
<p>A large number of ACVR1/ALK2 inhibitors were previously synthesised by Paul Brenner’s team (Target Discovery Center, University of Oxford) for the purpose of treating Fibrodysplasia Ossificans Progressiva (FOP). Although these compounds were not designed with blood-brain-barrier permeability in mind, they can serve as good bench-marks for my cellular assays. Therefore, 30 of these legacy compounds were chosen to be tested before other new bespoke Diffused Intrinsic Pontine Glioma (DIPG) compounds from Ontario Institute for Cancer Research (OICR) and Charles River Laboratories (CRL). Inhibition of TGFBR1/ALK5 leads to cardiac toxicity. Since I have not yet been able to establish a robust nanoBRET target engagement assay for TGFBR1/ALK5, I will have to resort to dual luciferase promoter assay (orthologous assay) for the time being.</p> <p>For my corresponding opennotebook post, please visit the following page:</p> <p>https://openlabnotebooks.org/determining-the-dual-luciferase-alk5-ic50-values-of-30-legacy-acvr1-alk2-inhibitors/</p>
Determining whether bespoke nanoBRET tracers work with ALK5
<p>I have always wanted to establish nanoBRET target engagement assay for ALK5. Relative to dual luciferase promoter assay and immunofluorescent staining, nanoBRET is many times faster. With a robust ALK5 nanoBRET assay, I will be able to rapidly screen for cellular off-target activity.</p> <p> </p> <p>Since none of the commercial nanoBRET tracers worked with ALK5, we attempted to generate our own tracers. We chose to create these bespoke tracers based on M4K1046 because it has cellular IC50 of ~50nM for ALK5. David Drewry has helped to design linkers that will attach fluorophores (nanoBRET energy acceptor) to the solvent-facing end of M4K1046. Based on known structures, additional bulk in this region should not hinder the binding of the compounds to ALK5. Two versions of M4K1046 (with different linker length) were synthesised by the M4K pharma chemist team in OICR. They were subsequently sent to Carrow Wells for conjugation to nanoBRET fluorophore.</p> <p>For more description of the project, please visit my blog posts:</p> <p><a href="https://openlabnotebooks.org/determining-whether-bespoke-tracers-work-with-alk5-in-nanobret-target-engagement-assay/">https://openlabnotebooks.org/determining-whether-bespoke-tracers-work-with-alk5-in-nanobret-target-engagement-assay/</a></p>
GDF11 rapidly increases lipid accumulation in liver cancer cells through ALK5-dependent signaling
GEO Series GSE165842. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Determine the role of ALK5 signaling in murine brain pericytes at embryonic day E13.5
GEO Series GSE268575. Mus musculus. 24 samples. Type: Expression profiling by high throughput sequencing.
Mitochondrial dysfunction induces ALK5-SMAD2-mediated hypovascularization and arteriovenous malformations in mouse retinas
GEO Series GSE172230. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
Determine the role of ALK5 signaling in human brain pericytes treated with TGFβ.
GEO Series GSE269066. Homo sapiens. 16 samples. Type: Expression profiling by high throughput sequencing.
Transcriptional profiling of skin tumor samples from mice with combintions of Braf, Alk5, p53, Tgfbr2 and Kras mutations
GEO Series GSE199070. Mus musculus. 40 samples. Type: Expression profiling by high throughput sequencing.
Gene expression profiling of the tongue bud from Alk5 mutant mouse models
GEO Series GSE52358. Mus musculus. 8 samples. Type: Expression profiling by array.
Gene expression profiling of the mandibular arch from Alk5 mutant mouse models
GEO Series GSE52357. Mus musculus. 8 samples. Type: Expression profiling by array.
Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 mutant
GEO Series GSE92420. Mus musculus. 24 samples. Type: Expression profiling by array.
Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 and TGFBR2 mutants
GEO Series GSE93333. Mus musculus. 152 samples. Type: Expression profiling by array.
Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 mutant (or control) at embryo stage E13.5
GEO Series GSE121200. Mus musculus. 32 samples. Type: Expression profiling by array.
Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 mutant (or control) at embryo stage E12.5
GEO Series GSE121193. Mus musculus. 24 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.