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21 results for “Absolute Quantification”
Dataset related to the article "Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR"
<p>This record contains raw data related to the article "Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR"</p> <p>Circulating microRNAs (miRNA) have been proposed as specific biomarkers for several diseases. Quantitative Real-Time PCR (RT-qPCR) is the gold standard technique currently used to evaluate miRNAs expression from different sources. In the last few years, digital PCR (dPCR) emerged as a complementary and accurate detection method. When dealing with gene expression, the first and most delicate step is nucleic-acid isolation. However, all currently available protocols for RNA extraction suffer from the variable loss of RNA species due to the chemicals and number of steps involved, from sample lysis to nucleic acid elution. Here, we evaluated a new process for the detection of circulating miRNAs, consisting of sample lysis followed by direct evaluation by dPCR in plasma from healthy donors and in the cardiovascular setting. Our results showed that dPCR is able to detect, with high accuracy, low-copy-number as well as highly expressed miRNAs in human plasma samples without the need for RNA extraction. Moreover, we assessed a known myocardial infarction-related miR-133a in acute myocardial infarct patients vs. healthy subjects. In conclusion, our results show the suitability of the extraction-free quantification of circulating miRNAs as disease markers by direct dPCR.</p>
Absolute Quantification of Coronary Flow Reserve by Stress Perfusion MRI
ClinicalTrials.gov study NCT01655043. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification: data and code example
<p>Minimal code example, raw and derived data for the publication on 'Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification'.</p>
Ammonia N-13 Myocardial Blood Flow Absolute Quantification by PET in Patients With Known or Suspected CAD (Ammonia MAP)
ClinicalTrials.gov study NCT04343209. IPD Sharing: Not stated. Countries: 1. Publications: 10.
Absolute quantification of single-base m6A methylation in the mammalian transcriptome
GEO Series GSE210563. Homo sapiens; Mus musculus. 58 samples. Type: Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing.
Method for absolute quantification of microbial communities by using both microarrays and competitive PCR
GEO Series GSE125085. Bacteria; Acinetobacter baumannii; Escherichia coli; Staphylococcus aureus; Staphylococcus epidermidis; Bacillus cereus; Schaalia odontolytica; Fusobacterium nucleatum subsp. nucleatum; Campylobacter rectus; Helicobacter pylori; Pseudomonas aeruginosa; Aggregatibacter actinomycetemcomitans; Phocaeicola vulgatus; Capnocytophaga gingivalis; Deinococcus radiodurans; Streptococcus mutans; Streptococcus intermedius; Cutibacterium acnes; Neisseria meningitidis; Porphyromonas gingivalis; Fusobacterium nucleatum; Clostridium beijerinckii; Lactobacillus gasseri; Tannerella forsythia; Treponema denticola; Cereibacter sphaeroides; Streptococcus gordonii; Streptococcus agalactiae; Enterococcus faecalis; Bifidobacterium adolescentis; Homo sapiens; Prevotella intermedia; Prevotella nigrescens. 178 samples. Type: Other.
GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution
GEO Series GSE233875. Homo sapiens. 2 samples. Type: Other.
Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs
GEO Series GSE56586. Xenopus laevis. 13 samples. Type: Expression profiling by high throughput sequencing.
Absolute transcription-start nucleotide m6Am stoichiometry quantification by CROWN-Seq
GEO Series GSE233655. Homo sapiens. 26 samples. Type: Other.
Absolute Quantification in Bone Scintigraphy
ClinicalTrials.gov study NCT04671238. IPD Sharing: NO. Countries: 1. Publications: 0.
Absolute quantification of miRNAs
GEO Series GSE15835. Rattus norvegicus; synthetic construct; Mus musculus; Homo sapiens. 6 samples. Type: Non-coding RNA profiling by array.
Absolute quantification of CD34(+)/CD133(-) hematopoietic stem cells
GEO Series GSE15831. Mus musculus; Homo sapiens; Rattus norvegicus; synthetic construct. 3 samples. Type: Non-coding RNA profiling by array.
Absolute quantification of B cell transcriptome using ERCC spiked-in RNA
GEO Series GSE83734. Mus musculus. 14 samples. Type: Expression profiling by high throughput sequencing.
Absolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation
GEO Series GSE114832. Drosophila melanogaster. 10 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Absolute quantification of cohesin, CTCF and their regulators in human cells
GEO Series GSE126990. Homo sapiens. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Genome-wide absolute quantification of chromatin looping
GEO Series GSE286495. Mus musculus. 4 samples. Type: Other.
An absolute quantification atlas of small non-coding RNAs across mammalian tissues and cell lines
GEO Series GSE279145. Arabidopsis thaliana; Mus musculus; Macaca fascicularis; Homo sapiens. 344 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Absolute quantification of protein production reveals principles underlying protein synthesis rates
GEO Series GSE53767. Escherichia coli. 4 samples. Type: Expression profiling by high throughput sequencing.
LOESS-Corrected UMI Counts Enable Absolute RNA Quantification in Single-Cell Transcriptomics
GEO Series GSE291906. Rattus norvegicus. 1 samples. Type: Expression profiling by high throughput sequencing.
Mild and ultrafast GLORI enables absolute quantification of m6A methylome from low-input samples
GEO Series GSE270643. Homo sapiens; Mus musculus. 47 samples. Type: Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing; Other.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.