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20 results for “Alexa”
SPAD-FCS data of freely diffusing Alexa 488 AB
<p>Confocal laser-scanning microscopy based fluorescence fluctuation spectroscopy (FFS) data set of freely diffusing Alexa 488 linked to an antibody. Excitation at 485 nm, 100x/1.4 Leica objective, detection with a 5x5 pixel SPAD array detector. The file "05_Alexa488AB_20ugmL_P60percP_SPAD.7z" contains a binary data file with the raw data of a 200 s FFS measurement. For each time bin of 500 ns, the fluorescence photon counts in each of the 25 pixels are stored. The text file "05_Alexa488AB_20ugmL_P60percP_SPAD_info.txt" contains the metadata. The file "05_Alexa488AB_20ugmL_P60percP_SPAD_xcorrs.7z" contains all 625 cross-correlations between I_1(t) and I_2(t), with I_1 and I_2 the intensity traces of any of the 25 pixels.</p>
Figure 44. Distribution map for Chrysina alexae n in Four new species of Chrysina Kirby (Coleoptera: Scarabaeidae: Rutelinae) from Guatemala and Honduras
Figure 44. Distribution map for Chrysina alexae n. sp., C. antonkozlovi n. sp., C. maishei n. sp. and C. woodruffi n. sp. in Guatemala and Honduras.
Alexa top 240 sites
<p>The Alexa top 240 sites for reproducing results from "FlexHTTP: An Intelligent and Scalable HTTP Version Selection System. EuroMLSys'22"</p>
High-throughput combinatorial droplet generation by sequential spraying: Fig 2, Alexa Fluor 594
<p>Images for figure 2 for Alexa Fluor portion of of "High-throughput combinatorial droplet generation by sequential spraying," with accomanying code at https://github.com/RenaFukuda99/High-throughput-combinatorial-droplet-generation-by-sequential-spraying. </p>
Example raw dSTORM data of Brp (Alexa Fluor532) and Unc-13 (Alexa Fluor647)
<p>This data set contains example raw <em>d</em>STORM data of Brp (Alexa Fluor532) and Unc-13 (Alexa Fluor647), recorded at <em>Drosophila melanogaster</em> male 3<sup>rd</sup> instar larval neuromuscular junctions at muscle 6/7. They are part of the dataset reported in the manuscript entitled "Endogenous tagging of Unc-13 reveals nanoscale reorganization at active zones during presynaptic homeostatic potentiation" and where made available during the review process at journal Frontiers in Cellular Neuroscience.</p>
FIGURE 2. Alexa duckeana G.S in Alexa duckeana (Leguminosae-Papilionoideae): a new species from the Brazilian Amazon
FIGURE 2. Alexa duckeana G.S. da Silva & Mansano. A. Leaves. B. View of the inflorescence in the canopy. C. Apical view of the inflorescence showing the arrangement of floral buds. D. Inflorescence with an anthetic flower. E. Flower in anthesis with detail of the petals, stamens, gynoecium, and nectariferous disc. F. Flower with detail of the stamens and gynoecium. Photos: A–F: Guilherme Silva.
FIGURE 4. Alexa grandiflora Ducke. A in Alexa duckeana (Leguminosae-Papilionoideae): a new species from the Brazilian Amazon
FIGURE 4. Alexa grandiflora Ducke. A. Flower in pre-anthesis showing the apex of the petals, stamens, and gynoecium. B. Flower in anthesis. C. Lateral view of an anthetic flower showing the calyx, anthers, style, and stigma. D. Flower in anthesis demonstrating the difference between the vexillum, wing, and keel petals. Photos: A. Guilherme Silva; B–D: Marcus Falcao.
FIGURE 1. Alexa duckeana G.S in Alexa duckeana (Leguminosae-Papilionoideae): a new species from the Brazilian Amazon
FIGURE 1. Alexa duckeana G.S. da Silva & Mansano. A. Flowering branch. B. Detail of leaflet blade base. C. Inflorescence with flowers in anthesis. D. Calyx with lobe details. E. Flower with the petals and stamens removed, showing the gynoecium. F. The external face of a petal. G. Stamen with detail of the filament insertion on the anther (dorsifixed). H. Gynoecium showing the indumentum of the ovary. I. Fruit demonstrating the persistent calyx. (A–E and I drawn from: Silva G.S. 528; F-H and J from: Silva G.S. 533). Illustrated by Marcus Falcão.
FIGURE 3 in Alexa duckeana (Leguminosae-Papilionoideae): a new species from the Brazilian Amazon
FIGURE 3. Geographical distribution and habitat of Alexa duckeana. A. Map of geographic distribution of Alexa duckeana, Alexa grandiflora and Alexa wachenheimii in the Amazon. B–C. Habitat of Alexa duckeana. Photos: Jone Carlos Ferreira Neves Junior.
Polarisation camera dSTORM datasets of actin in fixed HeLa cells labeled with phalloidin-Alexa Fluor 488
<p>Polarisation camera dSTORM dataset of the actin of fixed HeLa cells labeled with phalloidin-Alexa Fluor 488.</p> <p><strong>Image acquisition was performed as follows:</strong></p> <p>Imaging was performed on a widefield microscope equipped with a polarisation camera (CS505MUP, Thorlabs). The sample was excited using a 488 nm laser at quasi-TIRF, with a measured power density at the image plane of <span>5.04 kW/cm^2</span>. A single long-pass dichroic (Di02-R488, Semrock) was used to seperate fluorescence from the excitation. The emission was filtered using a long-pass (BLP01-488R, Semrock) and a bandpass filter (FF01-582/64, Semrock) before detection. An exposure time of 30 ms was used.</p> <p><strong>Samples were prepared as follows:</strong></p> <p><span>Cell culture: </span>HeLa TDS cells were cultured in DMEM (Gibco, Invitrogen) supplemented with 10 % Fetal Bovine Serum (FBS, Life Technologies), 1 % penicillin/streptomycin (Life Technologies), and 1 % glutamine (Life Technologies) at 37 °C + 5 % CO_2. Cells were periodically tested for mycoplasma contamination and passaged 3 times per week. Cells were plated at low density on high-precision glass coverslips (MatTek, P35G-0.170-14-C) 1 day prior to fixation for dSTORM experiments.</p> <p><span>Labeling:</span> Cells were simultaneously fixed and permeabilized in cytoskeleton buffer (CBS, 10 mM MES, 138 mM KCl, 3 mM MgCl_2, 2 mM EGTA, 4.5 % sucrose w/v, pH 7.4), + 4 % paraformaldehyde (PFA) and 0.2 % Triton for 6 minutes at 37 °C, and further fixed in CBS + 4 % PFA for 14 minutes at 37 °C. Post-fixation, cells were washed x3 in PBST (PBS supplemented with 0.1 % Tween) and permeabilized a second time in PBS + 0.5 % Triton for 5 minutes at room temperature. The samples were then washed 3 times in PBST and blocked for 30 minutes in 5 % BSA. Cells were washed x3 in PBST and then incubated with Alexa Fluor™ 488 Phalloidin (A12379, Invitrogen, 1:50 in PBS) for 1 h in the dark, followed by x2 washes in PBS. Prior to dSTORM imaging, PBS was replaced with dSTORM imaging buffer (base buffer consisting of 0.56 M glucose, 50 mM Tris (pH 8.5), and 10 mM NaCl supplemented with 5 U/mL pyranose oxidase (Sigma, P4234), 10 mM cysteamine (Sigma, 30070), 40 µg/mL catalase (Sigma, C100) and 2 mM cyclooctatetraene (Sigma, 138924).</p>
Polarisation camera dSTORM datasets of actin in fixed HeLa cells labeled with phalloidin-Alexa Fluor 647
<p>Polarisation camera dSTORM dataset of the actin of fixed HeLa cells labeled with phalloidin-Alexa Fluor 647.</p> <p><strong>Image acquisition was performed as follows:</strong></p> <p>Imaging was performed on a widefield microscope equipped with a polarisation camera (CS505MUP, Thorlabs). The sample was excited using a 638 nm laser at quasi-TIRF, with a measured power density at the image plane of 3.51 kW/cm^2. A multiband dichroic (Di03-R405/488/561/635-t1, Semrock) was used to seperate fluorescence from the excitation. The emission was filtered using a long-pass filter (BLP01-635R, Semrock) before detection. An exposure time of 30 ms was used.</p> <p><strong>Samples were prepared as follows:</strong></p> <p>Cell culture: HeLa TDS cells were cultured in DMEM (Gibco, Invitrogen) supplemented with 10 % Fetal Bovine Serum (FBS, Life Technologies), 1 % penicillin/streptomycin (Life Technologies), and 1 % glutamine (Life Technologies) at 37 °C + 5 % CO_2. Cells were periodically tested for mycoplasma contamination and passaged 3 times per week. Cells were plated at low density on high-precision glass coverslips (MatTek, P35G-0.170-14-C) 1 day prior to fixation for dSTORM experiments.</p> <p>Labeling: Cells were simultaneously fixed and permeabilized in cytoskeleton buffer (CBS, 10 mM MES, 138 mM KCl, 3 mM MgCl_2, 2 mM EGTA, 4.5 % sucrose w/v, pH 7.4), + 4 % paraformaldehyde (PFA) and 0.2 % Triton for 6 minutes at 37 °C, and further fixed in CBS + 4 % PFA for 14 minutes at 37 °C. Post-fixation, cells were washed x3 in PBST (PBS supplemented with 0.1 % Tween) and permeabilized a second time in PBS + 0.5 % Triton for 5 minutes at room temperature. The samples were then washed 3 times in PBST and blocked for 30 minutes in 5 % BSA. Cells were washed x3 in PBST and then incubated with Alexa Fluor™ 647 Phalloidin (A22287, Invitrogen, 1:50 in PBS) for 1 h in the dark, followed by x2 washes in PBS. Prior to dSTORM imaging, PBS was replaced with dSTORM imaging buffer (base buffer consisting of 0.56 M glucose, 50 mM Tris (pH 8.5), and 10 mM NaCl supplemented with 5 U/mL pyranose oxidase (Sigma, P4234), 10 mM cysteamine (Sigma, 30070), 40 µg/mL catalase (Sigma, C100) and 2 mM cyclooctatetraene (Sigma, 138924).</p>
Input files for molecular dynamics simulations of holo CaM tagged with Alexa Fluor 488 and Texas Red dyes using Amber20
<p>Here we share the input files for molecular dynamics simulations of holo CaM tagged with Alexa Fluor 488 and Texas Red dyes using Amber20, as well as the TrESP charges used in TrESP-MMPol electronic coupling calculations of FRET properties. Links to the TrADA tool used to derive TrESP charges and the Trespcoup software used to compute electronic couplings for FRET are indicated below:</p> <div> <div> <div> <p>Cupellini, L., Jurinovich, S., & Mennucci, B. (2024). TraDA - Transition Density Analyzer. Zenodo. https://doi.org/10.5281/zenodo.10966411</p> <p>Cupellini, L., Lipparini, F., & Cignoni, E. (2024). trespcoup - Software to compute TrEsp couplings. Zenodo. https://doi.org/10.5281/zenodo.10966391</p> </div> </div> </div>
Alexa Top 500 global sites
<p>The Alexa top 500 sites for reproducing results from "FlexHTTP: An Intelligent and Scalable HTTP Version Selection System."</p>
At-Home Subcutaneous Immunoglobulin Replacement Therapy Using Alexa Skill
ClinicalTrials.gov study NCT06150534. IPD Sharing: YES. Countries: 1. Publications: 0.
The dataset of the paper titled "Human Value Requirements in AI Systems: Empirical Analysis of Amazon Alexa"
<p>This package includes the dataset (user reviews) of Amazon Alexa and the corresponding labels of human values used in this paper which was submitted to EmpiRE 2023. It also includes the details of the results with some visualizations.</p>
Alexa, What is Eliquis and Xarelto?
ClinicalTrials.gov study NCT04790773. IPD Sharing: NO. Countries: 1. Publications: 0.
Testing the Feasibility of the DASH Cloud Intervention Using Amazon Alexa
ClinicalTrials.gov study NCT03455946. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
A Post-Marketing Study of Alexa Medium for Сorrection of the Lips Volume Loss
ClinicalTrials.gov study NCT06961305. IPD Sharing: UNDECIDED. Countries: 2. Publications: 0.
Use of Alexa as a Cognitive Aid for Emergency Front Of Neck Access (FONA)
ClinicalTrials.gov study NCT05635773. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Clinical Investigation of the Medical Device "Alexa Volume" for Correction of Midface Volume Loss
ClinicalTrials.gov study NCT06984419. IPD Sharing: Not stated. Countries: 2. Publications: 0.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.