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18 results for “Alternaria brassicicola”
RNA-Seq analysis to identify differentially expressed genes in top and bottom leaves under Alternaria brassicicola infection
<p>The broccoli plants were infected with Alternaria brassicicola and RNA samples were extracted for control and inoculated plants at 10 days post inoculation. </p>
Data from: Characterization of glutathione transferases involved in the pathogenicity of Alternaria brassicicola
Background: Glutathione transferases (GSTs) represent an extended family of multifunctional proteins involved in detoxification processes and tolerance to oxidative stress. We thus anticipated that some GSTs could play an essential role in the protection of fungal necrotrophs against plant-derived toxic metabolites and reactive oxygen species that accumulate at the host-pathogen interface during infection. Results: Mining the genome of the necrotrophic Brassica pathogen Alternaria brassicicola for glutathione transferase revealed 23 sequences, 17 of which could be clustered into the main classes previously defined for fungal GSTs and six were 'orphans'. Five isothiocyanate-inducible GSTs from five different classes were more thoroughly investigated. Analysis of their catalytic properties revealed that two GSTs, belonging to the GSTFuA and GTT1 classes, exhibited GSH transferase activity with isothiocyanates (ITC) and peroxidase activity with cumene hydroperoxide, respectively. Mutant deficient for these two GSTs were however neither more susceptible to ITC nor less aggressive than the wild-type parental strain. By contrast mutants deficient for two other GSTs, belonging to the Ure2pB and GSTO classes, were distinguished by their hyper-susceptibility to ITC and low aggressiveness against Brassica oleracea. In particular AbGSTO1 could participate in cell tolerance to ITC due to its glutathione-dependent thioltransferase activity. The fifth ITC-inducible GST belonged to the MAPEG class and although it was not possible to produce the soluble active form of this protein in a bacterial expression system, the corresponding deficient mutant failed to develop normal symptoms on host plant tissues. Conclusions: Among the five ITC-inducible GSTs analyzed in this study, three were found essential for full aggressiveness of A. brassicicola on host plant. This, to our knowledge is the first evidence that GSTs might be essential virulence factors for fungal necrotrophs.
Data from: Characterization of glutathione transferases involved in the pathogenicity of Alternaria brassicicola
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The transcription factor Amr1 induces melanin biosynthesis and suppresses virulence in Alternaria brassicicola
GEO Series GSE36781. Alternaria brassicicola. 2 samples. Type: Expression profiling by high throughput sequencing.
Transcriptional responses of Bdtf1-deletion mutant to the phytoalexin brassinin in the necrotrophic fungus Alternaria brassicicola
GEO Series GSE59195. Alternaria brassicicola. 12 samples. Type: Expression profiling by high throughput sequencing.
Transcription factor AbVf19 induces hydrolytic enzyme genes during pathogenesis in Alternaria brassicicola
GEO Series GSE33148. Brassica oleracea. 6 samples. Type: Expression profiling by high throughput sequencing.
Fungal-Specific Transcription Factor AbPf2 Activates Pathogenicity in Alternaria brassicicola
GEO Series GSE38984. Alternaria brassicicola. 4 samples. Type: Expression profiling by high throughput sequencing.
Dual-transcritome analysis of germinating Arabidopsis seeds in response to necrotrophic fungus Alternaria brassicicola
GEO Series GSE199977. Arabidopsis thaliana. 18 samples. Type: Expression profiling by high throughput sequencing.
Responses tophytoalexins in the necrotrophic fungus Alternaria brassicicola
GEO Series GSE140386. Alternaria brassicicola. 40 samples. Type: Expression profiling by array.
Responses to hydric stress in the seed-borne necrotrophic fungus Alternaria brassicicola
GEO Series GSE133507. Alternaria brassicicola. 24 samples. Type: Expression profiling by array.
Brassica oleracea-Alternaria brassicicola interaction during the early stages of leaf infection
GEO Series GSE155051. Arabidopsis thaliana; Brassica oleracea var. capitata. 8 samples. Type: Expression profiling by array.
Responses of Arabidopsis immune signaling mutants to Alternaria brassicicola infection
GEO Series GSE50526. Arabidopsis thaliana. 29 samples. Type: Expression profiling by array.
RNA-Seq analysis to identify differentially expressed genes in older and younger leaves of broccoli under Alternaria brassicicola infection
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Alternaria brassicicola infection regulates miRNAs in Arabidopsis thaliana
GEO Series GSE83488. Arabidopsis thaliana. 27 samples. Type: Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing.
Alternaria brassicicola infection regulates miRNAs in Arabidopsis thaliana [sRNA]
GEO Series GSE83487. Arabidopsis thaliana. 18 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Alternaria brassicicola infection regulates miRNAs in Arabidopsis thaliana [mRNA]
GEO Series GSE83478. Arabidopsis thaliana. 9 samples. Type: Expression profiling by high throughput sequencing.
Dual-transcriptome of post-germinating Arabidopsis thaliana mutant lines infected by Alternaria brassicicola
GEO Series GSE214602. Arabidopsis thaliana. 42 samples. Type: Expression profiling by high throughput sequencing.
Arabidopsis mutants treated with flg22, Alternaria brassicicola, Methyl jasmonate and Pst DC3000 bacteria
GEO Series GSE17464. Arabidopsis thaliana. 99 samples. Type: Expression profiling by array.
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