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26 results for “Ammodytes”
DATASET - Mass Spectrometry - Snake venom proteomics of island and mainland V. ammodytes populations from North Macedonia
<p><strong><span>This DATASET collection includes the mass spectrometry files for proteomics venom investigation of island and mainland <em>V. ammodytes</em> populations from North Macedonia.</span></strong></p> <p><strong><span>Sample list:</span></strong></p> <ol> <li><span>Island - adult - male</span></li> <li><span>Island - adult - female</span></li> <li><span>Island - juvenile</span></li> <li><span>Island - subadult</span></li> <li><span>Mainland - adult</span></li> <li><span>Mainland - subadult</span></li> <li><span>Mainland - juvenile</span></li> </ol> <p><strong><span>Folders 01-07 - BOTTOM-UP PROTEOMICS</span></strong><span>: The venom pools were investigated by the bottom-up "snake venomics" (labelled as SVX) approach and in short: separated by RP-HPLC, followed by SDS-PAGE separation and the single bands were in-gel processed by DTT, IAC and finally o/n tryptic digested. Samples submitted to HPLC-MS/MS. Early peptidic fractions of the first HPLC run were directly submitted to HPLC-MS/MS analytic w/o further gel procession. Folders 01 to 07 include the MS and MS/MS spectra of the <em>V. ammodytes</em> sample pools from different populations. Files are included as RAW and MZML format.</span></p> <p><span>Used instrument: LTQ Orbitrap XL mass spectrometer (Thermo, Bremen, Germany) with an Agilent 1260 HPLC system (Agilent Technologies, Waldbronn, Germany) using a reversed-phase Grace Vydac 218MS C18 (2.1 × 150 mm; 5 </span><span>μ</span><span>m particle size) column.</span></p> <p><span>Modifications: UNIMOD:4 - \"Iodoacetamide derivative.\"</span></p> <p><span>Used protein database: Uniprot_8750_serpentes_CanNIso_2674_entries_220210_cRAP_220210.fasta</span></p>
Figure 4 in Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)
Figure 4. Gel photograph showing the electrophoretic separation of the venom protein sample obtained from the 55-cm-long Vipera kaznakovi specimen, together with its densitometric tracing curve. For further explanation, see caption of Figure 2.
Figure 1 in Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)
Figure 1. Polyacrylamide gel electrophoresis of venoms of V. kaznakovi of different lengths. A. 16.5 cm, B. 30 cm, C. 55 cm (S: Start, junction between the stacking and separation gels).
Figure 3 in Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)
Figure 3. Gel photograph showing the electrophoretic separation of the venom protein sample obtained from the 30-cm-long Vipera kaznakovi specimen, together with its densitometric tracing curve. For further explanation, see caption of Figure 2.
Figure 8 in Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)
Figure 8. Gel photograph showing the electrophoretic separation of the venom protein sample obtained from the 51.5-cm-long Vipera ammodytes specimen, together with its densitometric tracing curve. For further explanation, see caption of Figure 2.
Figure 5 in Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)
Figure 5. Polyacrylamide gel electrophoresis of venoms of V. ammodytes of different lengths. A. 28.5 cm, B. 36.7 cm, C. 51.5 cm (S: Start, junction between the stacking and separation gels).
FIGURE 8 in Assessment of the Linnaean type material of the Nose-horned viper, Vipera ammodytes (Linnaeus, 1758)
FIGURE 8. Page from the Royal Swedish Academy of Sciences (KVA) donation catalogue with the Carleson donation entry on 4 October 1746.
FIGURE 4. Coluber ammodytes specimen UPSZTY 95 in Assessment of the Linnaean type material of the Nose-horned viper, Vipera ammodytes (Linnaeus, 1758)
FIGURE 4. Coluber ammodytes specimen UPSZTY 95, collected by Carleson. Details of the lateral side of the head right and left; dorsolateral view of the whole specimen; frontal view of the rostral area; and dorsal view of the head.
FIGURE 7 in Assessment of the Linnaean type material of the Nose-horned viper, Vipera ammodytes (Linnaeus, 1758)
FIGURE 7. Relative position of the lectotype in the projection of the first and the second correspondence axes (qualitative data). Use of names in this figure follows Tomovic (2006) and Hempel et al. (2018).
FIGURE 6 in Assessment of the Linnaean type material of the Nose-horned viper, Vipera ammodytes (Linnaeus, 1758)
FIGURE 6. Relative position of the lectotype in the projection of the first and the second canonical axes (size-adjusted morphometric and meristic data). Use of names in this figure follows Tomovic (2006) and Hempel et al. (2018).
FIGURE 3 in Assessment of the Linnaean type material of the Nose-horned viper, Vipera ammodytes (Linnaeus, 1758)
FIGURE 3. Plate from the dissertation by Linnaeus & Sundius (1748) with Figure II depicting the Coluber ammodytes specimen.
Supplementary material 2 from: Thiel R, Knebelsberger T (2016) How reliably can northeast Atlantic sand lances of the genera Ammodytes and Hyperoplus be distinguished? A comparative application of morphological and molecular methods. ZooKeys 617: 139-164. https://doi.org/10.3897/zookeys.617.8866
Table S2 : Explanation note: Museum IDs and collection data for specimens of Ammodytes tobianus used for morphological analyses only.
Supplementary material 1 from: Thiel R, Knebelsberger T (2016) How reliably can northeast Atlantic sand lances of the genera Ammodytes and Hyperoplus be distinguished? A comparative application of morphological and molecular methods. ZooKeys 617: 139-164. https://doi.org/10.3897/zookeys.617.8866
Table S1 : Explanation note: Supplementary metadata for specimens used for both morphological and genetic analyses; Museum and Sample IDs are specimen identifiers, BOLD Process IDs are unique codes automatically generated for each record on BOLD, GenBank Accession NOs represent sequence identifiers.
FIGURE 2. Rhinaspis ammodytes velebitensis. Holotype, SMF 56627 in An account of the generic and specific names, and type specimens of viperid taxa described by Albert Franz Theodor Reuss (Squamata: Viperidae)
FIGURE 2. Rhinaspis ammodytes velebitensis. Holotype, SMF 56627. General view. Photograph by Michael Franzen.
FIGURE 1 in Assessment of the Linnaean type material of the Nose-horned viper, Vipera ammodytes (Linnaeus, 1758)
FIGURE 1 Section of pages 216–217 of Ed. 10 of 'Systema Naturae' describing Coluber ammodytes.
FIGURE 2 in Assessment of the Linnaean type material of the Nose-horned viper, Vipera ammodytes (Linnaeus, 1758)
FIGURE 2 Section of pages 376 of 'Systema Naturae' Ed. 12 describing Coluber ammodytes.
FIGURE 5 in Assessment of the Linnaean type material of the Nose-horned viper, Vipera ammodytes (Linnaeus, 1758)
FIGURE 5. Original jar with the label in Thunberg's handwriting.
Figure 2 in Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)
Figure 2. Gel photograph showing the electrophoretic separation of the venom protein sample obtained from the 16.5-cm-long Vipera kaznakovi specimen, together with its densitometric tracing curve (O.D.: Optical density, S: Start, junction between the stacking and separation gels).
Figure 7 in Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)
Figure 7. Gel photograph showing the electrophoretic separation of the venom protein sample obtained from the 36.7-cm-long Vipera ammodytes specimen, together with its densitometric tracing curve. For further explanation, see caption to Figure 2.
Figure 6 in Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)
Figure 6. Gel photograph showing the electrophoretic separation of the venom protein sample obtained from the 28.5-cm-long Vipera ammodytes specimen, together with its densitometric tracing curve. For further explanation, see caption to Figure 2.
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