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Fig. 1 in Molecular evidence of hybridisation in two invasive species of Pomacea (Gastropoda: Ampullariidae) in Peninsular Malaysia

Fig. 1. Geographical location and habitat type of sampling sites of Pomacea species in Peninsular Malaysia.

Fig. 4 in Molecular evidence of hybridisation in two invasive species of Pomacea (Gastropoda: Ampullariidae) in Peninsular Malaysia

Fig. 4. Median-joining haplotype network of Pomacea canaliculata and P. maculata sequences from Peninsular Malaysia (N=108 from 90 individuals) and the native ranges (N=105) based on 409 nucleotides of the EF1α gene. The network shows the relationship between haplotypes from different geographic regions based on sequence similarity. Unique sequences within each individual were included in the alignment (sequences for homozygotes were not doubled). Node colours represent the (A) geographic location and (B) species identity of the sequences (see legends). Each node represents a unique haplotype and node size is proportional to the haplotype frequency. Branches between nodes indicate a single nucleotide substitution unless denoted by numerical values for multiple nucleotide substitutions. Red (A) and black (B) nodes represent hypothetical ancestors or unsampled haplotypes. Two major groups are framed in grey dotted lines; P. canaliculata and P. maculata.

Fig. 3 in Molecular evidence of hybridisation in two invasive species of Pomacea (Gastropoda: Ampullariidae) in Peninsular Malaysia

Fig. 3. Bayesian inference phylograms depicting relationship of P. canaliculata and P. maculata from Peninsular Malaysia and Pomacea spp. from other regions based on the (A) mitochondrial COI and (B) nuclear EF1α markers. Kuantan, Tasik ChinChin, Limbat Lembu, Subang Jaya, Putrajaya, Guar Cempedak, Pasir Gudang, Sekinchan, and Temoh refer to geographic locations in Peninsular Malaysia where specimens in this study were collected. Bayesian posterior probabilities/maximum likelihood bootstrap supports are indicated by nodal values. Pomacea difussa and P. scalaris were used to root the phylogenies. Pomacea canaliculata and P. maculata clades are highlighted in green and blue, respectively. Underlined taxa marked with '*' indicate interspecific heterozygous individuals whereas taxa in red and marked with '**' are COI-EF1α mito-nuclear incongruences.

Fig. 2 in Molecular evidence of hybridisation in two invasive species of Pomacea (Gastropoda: Ampullariidae) in Peninsular Malaysia

Fig. 2. Representative agarose gel electrophoresis image showing the ApaLI-digested EF1α amplicons for 14 specimens from Putrajaya. The single band, two-band, and three-band RFLP profiles indicate Pomacea canaliculata, P. maculata, and interspecific heterozygous hybrids, respectively.

Figure 4 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figure 4. Box plot of the snail's activity time (min) for all the activity and behaviour experiment, represented according to the sex of the experimental snail, the absence or presence of a partner and its sex.

Figure 1 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figure 1. Arrangement of aquaria used to assess the activity and behaviour of snails in the presence of a congener of the same or different sex. R: clock; T: thermometer.

Figure 3 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figure 3. Survivorship as a function of time (days) and treatment. The intersection with the horizontal line indicates the median of the survival time for each treatment. The rectangle (▯) that encloses the sex indicates the individual considered in the analysis in the treatments with both sexes. The crosses (x) indicate that the treatments were censored.

Figura 1 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figura 1. Arreglo de acuarios utilizado para evaluar la actividad y el comportamiento de los caracoles en función de la presencia de un congénere del mismo o de distinto sexo. R: reloj; T: termómetro.

Figura 2. A in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figura 2. A) Superposición digital de 200 imágenes tomadas durante el tercer cuarto del experimento de actividad y comportamiento utilizada para calcular el porcentaje de área explorada. R: reloj; T: termómetro. B) Simulación realizada para reconstruir el área explorada por el macho y la hembra por separado en el tratamiento (Ÿÿ) que incluía un caracol de cada sexo. Figure 2. A) Digital overlap of 200 images taken during the third quarter of the experiment of activity and behaviour used to calculate the percentage of explored area. R: clock; T: thermometer. B) Simulation carried out to reconstruct the area explored by the male and the female separately in the treatment (Ÿÿ) that included one snail of each sex.

Figura 4 in Mortalidad y comportamiento de búsqueda de pareja en Pomacea canaliculata (Caenogastropoda: Ampullariidae)

Figura 4. Diagrama de cajas y brazos del tiempo de actividad (min) de los caracoles para el experimento completo de actividad y comportamiento, representados en función del sexo del caracol experimental y de la ausencia o presencia de una pareja y del sexo de la misma.

FIG. 1. — A in Annotated catalogue of the types of Ampullariidae (Mollusca, Gastropoda) in the Muséum national d'Histoire naturelle, Paris, with lectotype designations

FIG. 1. — A, Ampullaria aulanieri Deville & Huppé, 1850,lectotype MNHN 23072, 48.2 mm; B, Ampullaria baeri Dautzenberg,1902, lectotype MNHN 4493, 27.0 mm; C, Ampullaria begini Morlet, 1889, syntype MNHN 4499, 21.4 mm; D, Ampullaria belizensis Crosse & Fischer in Fischer & Crosse, 1888, holotype MNHN 5139, 61.8 mm; E, Meladomus bloyeti Bourguignat, 1889, lectotype MNHN 4501, 63.3 mm; F, Ampullaria bruguieri Deshayes, 1830, lectotype MNHN 23074, 72.1 mm; G, Ampullaria carinata Olivier, 1804, lectotype MNHN 23075, 28.5 mm; H, Ampullaria castanea Deshayes, 1830, lectotype MNHN 4710, 42.2 mm.

FIG. 5. — A in Annotated catalogue of the types of Ampullariidae (Mollusca, Gastropoda) in the Muséum national d'Histoire naturelle, Paris, with lectotype designations

FIG. 5. — A, Lanistes neritoides Brown & Berthold, 1990, holotype MNHN 4515, 12.9 mm; B, Ampullaria neritoides d'Orbigny, 1835, probable syntype MNHN 4519, 97.2 mm; C, Afropomus balanoideus nimbae Binder, 1963, lectotype MNHN 4497, 18.8 mm; D, Ampullaria occlusa Crosse & Fischer in Fischer & Crosse, 1888, holotype MNHN 4514, 49.6 mm; E, Ampullaria ovata Olivier, 1804, syntype MNHN 4513, 64.3 mm; F, Ampullaria oviformis Deshayes, 1830, syntype MNHN 4512, 43.4 mm; G, Ampullaria perakensis Morgan, 1885, lectotype MNHN 4511, 33.6 mm; H, Ampullaria peristomata d'Orbigny, 1835,syntype MNHN 4509, 38.7 mm; I, Ampullaria pesmei Morlet, 1889, lectotype MNHN 4508, 30.2 mm.

FIG. 7. — A in Annotated catalogue of the types of Ampullariidae (Mollusca, Gastropoda) in the Muséum national d'Histoire naturelle, Paris, with lectotype designations

FIG. 7. — A, Ampullaria simplicula Mabille,1889, lectotype MNHN 4520, 29.1 mm; B, Lanistes ellipticus var. β trapeziformis Furtado, 1886, lectotype MNHN 23089, 52.8 mm; C, Ampullaria violacea Valenciennes, 1833, holotype MNHN 4530, 43.1 mm; D, Ampullaria virescens Deshayes in Bory de Saint-Vincent,1824,holotype MNHN 24513,54.7 mm (also lectotype of Ampullaria polita Deshayes,1830);E, Ampullaria wellesleyensis Morgan, 1885, lectotype MNHN 24514, 34.1 mm; F, Lanistes zambesianus Furtado, 1886,holotype MNHN 4525, 55.6 mm; G, Ampullaria zonata d'Orbigny, 1835, syntype MNHN 4523, 38.2 mm (also lectotype of Ampullaria spixii d'Orbigny, 1838).

FIG. 3. — A, Ampullaria elegans d in Annotated catalogue of the types of Ampullariidae (Mollusca, Gastropoda) in the Muséum national d'Histoire naturelle, Paris, with lectotype designations

FIG. 3. — A, Ampullaria elegans d'Orbigny, 1835, syntype MNHN 23081, 31.5 mm; B, Ampullaria turbinis var. erythrochila Dautzenberg & Fischer, 1905, lectotype MNHN 4738, 49.0 mm; C, Ampullaria eumicra Crosse & Fischer, 1890, lectotype MNHN 23082, 29.8 mm; D, Ampullaria gevesensis Deshayes, 1838,syntype MNHN 4473, 31.9 mm; E, Ampullaria (Lanistes) grasseti Morelet, 1863,probable syntype MNHN 4457, 29.5 mm; F, Ampullaria guyanensis Lamarck,1822, paralectotype MNHN 4459, 86.8 mm; G, Ampullaria insularum d'Orbigny, 1835, syntype MNHN 4463, 76.0 mm; H, Ampullaria intermedia Férussac in Quoy & Gaimard, 1825, lectotype MNHN 23083, 36.0 mm.

FIG. 2. — A in Annotated catalogue of the types of Ampullariidae (Mollusca, Gastropoda) in the Muséum national d'Histoire naturelle, Paris, with lectotype designations

FIG. 2. — A, Ampullaria castelnaudi Hupé, 1857, lectotype MNHN 23076, 103.9 mm; B, Ampullaria celebensis Quoy & Gaimard, 1834, lectotype MNHN 23077, 70.5 mm; C, Ampullaria chariensis Germain, 1905, lectotype MNHN 4478, 38.7 mm; D, Ampullaria columbiensis Reeve, 1856, syntype MNHN 4480, 36.8 mm; E, Ampullaria cousini Jousseaume, 1887, lectotype MNHN 4483, 43.0 mm; F, Ampullaria decocta Mabille, 1887, lectotype MNHN 23078, 29.7 mm; G, Ampullaria decussata Moricand, 1836, possible syntype MNHN 23079, 28.0 mm.

FIGURE 3. Uorticella veloxiiforme n in Description of Two New Species of the Genus Vorticella (Ciliophora: Peritrichia) Epibionts on Pomacea canaliculata (Mollusca: Ampullariidae: Gastropoda) in Southern Brazil

FIGURE 3. Uorticella veloxiiforme n. sp. silver nitrate-stained species and SEM photomicrographs: A. Picture showing a zooid with horizontal striation and the trochal band (arrow) (Bar 10 µm). B. SEM micrograph of the zooid showing pellicular pores (arrow) (Bar 2 µm). C. Silver nitrate-stained zooid showing the silver lines on the pellicle (arrowheads) (Bar 20 µm).

FIGURE 5. Uorticella ampullaria n in Description of Two New Species of the Genus Vorticella (Ciliophora: Peritrichia) Epibionts on Pomacea canaliculata (Mollusca: Ampullariidae: Gastropoda) in Southern Brazil

FIGURE 5. Uorticella ampullaria n. sp. silver nitrate-stained specimens and SEM photomicrographs: A. Picture showing details of the pores on the pellicle (arrow) (Bar 2 µm). B. SEM micrograph of the zooid showing the trochal band (arrow) (Bar 2.5 µm). C. Silver nitrate-stained zooid showing the silver lines on the pellicle (arrow) (Bar 4 µm). D. Silver nitrate-stained zooid showing the macronucleus (MAC), scopula(SC), and somatic myonemes (arrowhead).

FIGURE 2 in Description of Two New Species of the Genus Vorticella (Ciliophora: Peritrichia) Epibionts on Pomacea canaliculata (Mollusca: Ampullariidae: Gastropoda) in Southern Brazil

FIGURE 2. Schematic drawings of Uorticella veloxiiforme n. sp. and Uorticela ampullaria sp.n. in vivo and from protargolstained specimens: A. Live zooid of U. veloxiiforme showing the contractile vacuole (CV) and a J-shaped macronucleus (MAC) (Bar 15 µm). B. Protargol-stained zooid of U. veloxiiforme showing the oral poliknetids inside the infundibular region (P1, P2, P3) (Bar 10 µm). C. Live zooid of U. ampullaria showing the position of the two contractile vacuoles (CV) and a Cshaped macronucleus (MAC) (Bar 15 µm). D. Protargol-stained zooid of U. ampullaria showing the oral polykinetids inside the infundibulum (P1, P2, P3) (Bar 10 µm).

FIGURE 1 in Description of Two New Species of the Genus Vorticella (Ciliophora: Peritrichia) Epibionts on Pomacea canaliculata (Mollusca: Ampullariidae: Gastropoda) in Southern Brazil

FIGURE 1. Uorticella veloxiiforme live and protargol-stained specimens: A. Live zooids showing the contractile vacuole (CV), a J-shaped macronucleus and a spasmoneme inside the basal stalk (SM) (Bar 10 µm). B. Live zooid showing the position of the J-shaped macronucleus (MAC) (Bar 10 µm). C. Protargol-stained zooid showing the J-shaped macronucleus (MAC) (Bar 10 µm). D. Detail of a protargol-stained zooid showing the oral polykinetids (P1, P2, P3) inside the infundibulum (Bar 10 µm). E. Protargol-stained zooid showing the somatic myonemes (arrow) (Bar 10 µm).