Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
36
datasets available to search
ShareScore release 0.9.0
Dataset results
36 results for “Aphrophoridae”
Draft genome assembly version 1 of the meadow spittlebug Philaenus spumarius (Linnaeus, 1758) (Hemiptera, Aphrophoridae)
<p>We sequenced the genome of the meadow spittlebug, <em>Philaenus spumarius </em>(Linnaeus, 1758), the main insect vector of <em>Xylella fastidiosa </em>Wells et al. 1987 in Europe (Saponari et al., 2014), using 10x Chromium linked-reads. A single <em>P. spumarius</em> adult female from Portugal (Fontanelas, Sintra; GPS location: 38°50'15.75"N; 9°25'20.77"W), collected in September of 2018, was selected for genome sequencing. This population was initially surveyed for colour polymorphism in 1988 (Quartau & Borges, 1997) and was later included in phylogeographic and population genomic studies of this species (Rodrigues et al., 2014; Seabra et al., unpublished). It is also geographically close to the population from which the individual used for the first partial genome assembly was collected (Rodrigues et al., 2016). The availability of this previous genetic information contributed to the choice of this population as the source of genomic material for whole genome sequencing. A subset of males from the same collection date were analysed for genitalia morphology to confirm species identification, as the best diagnostic characters are the appendages of the aedeagus (Drosopoulos & Quartau, 2002).</p> <p>The genomic DNA of the <em>P. spumarius</em> adult from Sintra was extracted using Illustra Nucleon Phytopure kit according to the manufacturer’s instructions (GE Healthcare). We assessed the quality and concentration of the DNA using Femto fragment analyser (Agilent). 10x Chromium library preparation and Illumina genome sequencing (HiSeq X, 150bp paired-end) were performed by Novogene Bioinformatics Technology Co, Beijing, China, in accordance with standard protocols.</p> <p>To create the <em>de novo</em> 10x Chromium assembly we ran Supernova 2.1.1 (Weisenfeld et al., 2017) on the 10x Chromium linked-read data with default parameters, using 1.0 billion reads corresponding to 56X coverage. To improve the initial supernova assembly, we performed iterative scaffolding using all of the 10x raw data (2.3 billion of reads). We ran two rounds of Scaff10x (https://github.com/wtsi-hpag/Scaff10X), followed by mis-assembly detection and correction with Tigmint (Jackman et al., 2018). This was followed by a final round of scaffolding with ARCS (Yeo et al., 2018). The assembly was checked for contamination using the BlobTools pipeline (version 0.9.19; Laetsch and Blaxter 2017; Kumar et al., 2013) and k-mer content was analysed with the KAT comp tool (Mapleson et al., 2017). In order to perform these analyses, it was necessary to remove the 10x linked barcodes from the reads with the script process_10xReads.py (https://github.com/ucdavis-bioinformatics/proc10xG). We assessed the quality of our draft genome assembly by searching for conserved, single copy, arthropod genes (n=1,066) with Benchmarking Universal Single-Copy Orthologs (BUSCO) v3.0 (Waterhouse et al., 2018).</p> <p>With the above assembly procedure, we obtained a final assembly of 2.7 Gb, having a scaffold N50 length of 116 Kb (contig N50 = 18 Kb) and the longest scaffold was 3.7 Mb. The length of the assembly was consistent with the genome size estimated by flow cytometry (Rodrigues et al., 2016). The k-mer distribution indicated high heterozygosity, estimated at 2.3%. BlobTools analyses revealed the presence of contigs assigned to <em>Sodalis </em>spp. (Enterobacteriaceae), a symbiont in members of tribe Philaenini (Koga et al., 2013). These contigs were filtered from the final assembly. Gene completeness assessment shows that 956 (89.6%) among 1,066 BUSCOs were found as complete copies, with only 26 (2.4%) missing. Of the BUSCOs that were detected, 878 (82.4%) were complete and single-copy, 78 (7.3%) were complete and duplicated and 84 (7.9%) were fragmented.</p> <p>In conclusion, due in part to high (2.3%) heterozygosity levels, the <em>P. spumarius</em> version 1 genome assembly is highly fragmented. Nonetheless, the assembly is considered complete and is likely to contain the majority of the gene content of <em>P. spumarius.</em></p>
Linked collectors and determiners for: First Canadian record of Aphrophora salicina (Goeze, 1778) (Hemiptera: Aphrophoridae).
Natural history specimen data linked to collectors and determiners held within, "First Canadian record of Aphrophora salicina (Goeze, 1778) (Hemiptera: Aphrophoridae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="https://bionomia.net/dataset/d1a704c4-0660-4c10-9e14-c296f3125a83">https://bionomia.net/dataset/d1a704c4-0660-4c10-9e14-c296f3125a83</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/d1a704c4-0660-4c10-9e14-c296f3125a83">https://gbif.org/dataset/d1a704c4-0660-4c10-9e14-c296f3125a83</a>. Formatted as a Frictionless Data package.
Spittle Island - Aphrophoridae rearing method on floating platforms
<p>Method of laboratory rearing of field collected Aphrophoridae by confinement of juveniles-bearing plants in ventilated tubes. The tubes are placed on floating platforms that allow continuous water supply to the plants.</p>
Exploring the activity of Chrysoperla carnea (Neuroptera: Chrysopidae) and Beauveria bassiana (Ascomycota: Hypocreales) on Neophilaenus campestris (Hemiptera: Aphrophoridae), vector of Xylella fastidiosa
<p>Raw data and R codes from the study "Exploring the activity of Chrysoperla carnea (Neuroptera: Chrysopidae) and Beauveria bassiana (Ascomycota: Hypocreales) on Neophilaenus campestris (Hemiptera: Aphrophoridae), vector of Xylella fastidiosa"</p>
Figure 6 in A new species of Cladochaeta Coquillettı 1900 (Diptera: Drosophilidae) associated with Sphodroscarta trivirgata (Amyot & Servilleı 1843) (Auchenorrhyncha: Aphrophoridae) from the Brazilian Amazon rainforest
Figure 6. Cladochaeta amorimi sp. nov., puparium. (a) Entire puparium, dorsal view. Scale bar: 500 µm. (b) Anterior spiracle, dorsal view. Scale bar: 50 µm. (c) Posterior spiracle, dorsal view. Scale bar: 100 µm. (d) Rosettes of abdominal segment, ventral view. Scale bar: 100 µm. Arrows pointing A: anterior and L: lateral.
FIGURE 1 in Revision of the genus Ariptyelus Matsumura (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 1. Tegmen and wing: A, Ariptyelus arisanus, tegmen; B, A. arisanus, wing; C, A. auropilosus, tegmen; D, A. auropilosus, wing; ap, apical cell; AM, anal margin; BW, basal widest point; LBW, length from base to basal widest point; LMW, length from basal point to middle widest point; LT, length of tegmen; MW, middle widest point; PM, posterior margin; WCA, wing coupling apparatus.
FIGURE 4 in Revision of the genus Ariptyelus Matsumura (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 4. Ariptyelus auropilosus (Matsumura, 1907): A, Male genitalia, lateral view; B, pygofer, ventral view; C, left genital style, outer lateral side; D, the same, inner lateral side; E, aedeagus, phallobase, and connective, lateral view.
FIGURE 2 in Revision of the genus Ariptyelus Matsumura (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 2. Ariptyelus arisanus (Matsumura, 1942) and A. auropilosus (Matsumura, 1907), males: A, Dorsal view; B. lateral view; C. dorsal view; D. lateral view.
FIGURE 5 in Revision of the genus Ariptyelus Matsumura (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 5. Ariptyelus subauropilosus Shih and Yang, sp. nov.: A, Holotype, male, dorsal view; B, holotype, male, lateral view; C–F, paratype, male; C, tegmen; D, wing; E, D, brown pubescence on apical region of the wing; F, wing coupling apparatus with 3 hooks on the basal part of costal margin of the wing.
FIGURE 6 in Revision of the genus Ariptyelus Matsumura (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 6. Ariptyelus subauropilosus Shih and Yang, sp. nov.: A, Male genitalia, lateral view; B, pygofer, ventral view; C, left genital style, outer lateral side; D, the same, inner lateral side; E, aedeagus, phallobase, and connective, lateral view; aed, aedeagus.
FIGURE 4 in The genus Jembra Metcalf and Horton from Taiwan with descriptions of two new species and the nymph of J. taiwana sp. nov. (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 4. Jembra taiwana Shih, sp. nov.: A–B, the fifth instar nymph. A, dorsal view; B, lateral view. C–E, adult, Holotype, male: C, dorsal view; D, tegmen; E, wing.
FIGURE 5 in The genus Jembra Metcalf and Horton from Taiwan with descriptions of two new species and the nymph of J. taiwana sp. nov. (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 5. Jembra taiwana Shih, sp. nov.: A–B, the fourth instar nymph. A, head and pronotum, dorsal view; B, head, ventral view; C–H, the fifth instar nymph. C, head and pronotum, dorsal view; D, head, ventral view; E, anterior and posterior wing pads; F, coxal meracanthus of midleg; G, tibia and tarsus, hindleg; H. apical spines of tibia, hindleg; I–P, Holotype, adult, male: I, abdominal segments IX–XI, lateral view; J, abdominal segment IX, ventral view; K, coxal meracanthus of midleg; L, connective and genital styles, ventral view; M, aedeagus, lateral view; N, apex of aedeagus, caudal view; O, right genital style, outer side; P, the same, inner side; A1–A3, abdominal segmented 1–3; AWP, anterior wing pad; PWP, posterior wing pad.
FIGURE 1 in The genus Jembra Metcalf and Horton from Taiwan with descriptions of two new species and the nymph of J. taiwana sp. nov. (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 1. Jembra inouyei (Matsumura, 1942): A, meracanthus of right midleg, dorsal view; B, abdominal segments IX–XI, lateral view; C, abdominal segment IX, ventral view; D, right genital style, outer side; E, the same, inner side; F, connective, ventral view; G, aedeagus, dorsolateral view; H–J, apex of aedeagus. H, caudoventral view; I, caudal view; J, dorsal view; aed, aedeagus; con, connective; dp, dorsal process of pygofer; gs, genital styles; IX–XI, abdominal segments IX–XI; mer, meracanthus; phb, phallobase; vp, ventral process of pygofer; XIt, abdominal tergite XI; XIs, abdominal sternite XI.
FIGURE 2 in The genus Jembra Metcalf and Horton from Taiwan with descriptions of two new species and the nymph of J. taiwana sp. nov. (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 2. Jembra kuanae Shih, sp. nov.: A, Paratype, male, dorsal view; B–C, the same. B, lateral view; C, the first tarsus with apical spines arranged in 2 rows (shown as 2 arrowheads); D–E, head and pronotum, male, one of paratypes; F, middle coax with one meracanthus (mer) from ventrolateral side; G, flagellum with 2 types of sensory organs on ventrolateral side; H, basiconic sensilla (ba); I, coeloconic sensilla (co).
FIGURE 3 in The genus Jembra Metcalf and Horton from Taiwan with descriptions of two new species and the nymph of J. taiwana sp. nov. (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 3. Jembra kuanae Shih, sp. nov.: A, Holotype, male: A, head and pronotum, dorsal view; B, head, ventral view; C, tegmen; D, punctures of tegmen; E, Wing; F, right midleg, dorsal view; G, the same, ventral view; H, tibia and tarsi of left hindleg; I, abdominal segments IX–XI, lateral view; J, abdominal segment IX, ventral view; K, right genital style, inner side; L, the same, outer side; M, aedeagus, lateral view; N, apex of aedeagus, caudal view; O, aedeagus, dorsal view; P, aedeagus, ventral view; ap, apical cell.
FIGURES 20–29 in The aphrophorid spittlebugs of Iran (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURES 20–29. Distribution maps of Aphrophoridae species in Iran: 20. Poophilus costalis. 21. Aphrophora salicina. 22. Aphrophora alni. 23. Paraphilaenus notatus. 24. Lepyronia coleoptrata. 25. Neophilaenus lineatus. 26. Neophilaenus campestris. 27. Mesoptylus iranicus. 28. Philaenus spumarius (grey dots are only based on the literature and the specimens were not examined). 29. Philaenus elbursianus.
FIGURES 17–19 in The aphrophorid spittlebugs of Iran (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURES 17–19. Male genitalia of Aphrophoridae species of Iran: 17. Mesoptylus iranicus: a) aedeagus. b) stylus. c) anal tube. 18. Philaenus spumarius: a) aedeagus. b) stylus. c) anal tube. 19. Philaenus elbursianus: a) aedeagus. b) stylus. c) anal tube.
FIGURES 11–16 in The aphrophorid spittlebugs of Iran (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURES 11–16. Male genitalia of Aphrophoridae species of Iran: 11. Poophilus costalis: a) aedeagus. b,c) stylus. d) anal tube. e) genital plate. 12. Aphrophora alni: a) aedeagus. b) stylus. 13. Paraphilaenus notatus: a) aedeagus. b) stylus. 14. Lepyronia coleoptrata: a) aedeagus. b) stylus. c) anal tube. 15. Neophilaenus lineatus: a) aedeagus. b) stylus. 16. Neophilaenus campestris: a) aedeagus. b) stylus.
FIGURES 1–10 in The aphrophorid spittlebugs of Iran (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURES 1–10. Dorsal view of Aphrophoridae species of Iran: 1. Poophilus costalis. 2. Aphrophora salicina. 3. Aphrophora alni. 4. Forebody of Paraphilaenus notatus. 5. Lepyronia coleoptrata. 6. Neophilaenus lineatus. 7. Neophilaenus campestris. 8. Mesoptylus iranicus. 9. Philaenus spumarius. 10. Philaenus elbursianus.
FIGURE 3 in Revision of the genus Ariptyelus Matsumura (Hemiptera: Cercopoidea: Aphrophoridae)
FIGURE 3. Ariptyelus arisanus (Matsumura, 1942): A, Male genitalia, lateral view; B, pygofer, ventral view; C, left genital style, outer lateral side; D, the same, inner lateral side; E, aedeagus, phallobase, and connective, lateral view; aed, aedeagus; bmp, basal margin of pygofer in lateral view; con, connective; dp, dorsal process of pygofer; gs, genital styles; IX–XI, abdominal segments IX–XI; LAGS, length of genital style; LBWP, the length of basal wide of pygofer; LP, length of pygofer; LPML, length of pygofer in middle line; LVP, length of vp in lateral view; LVVP, length of vp in ventral view; LWVP, length of the widest part of vp in lateral view; LVMP, length of ventral margin of pygofer; phb, phallobase; vp, ventral process of pygofer; WAGS, width of apical part of genital style; XIt, abdominal tergite XI; XIs, abdominal sternite XI; WP, wide of pygofer.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.