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Fig. 1 in Ontogenetic development of tetra Astyanax lacustris (Characiformes: Characidae)

Fig. 1. Development of Astyanax lacustris during the yolk-sac larval stage: a. newly hatched larva, with adhesive gland (A), eyes with little pigmentation (B), the optic vesicle (C), and no anal opening (D); b. increased pigmentation of the eye (E), increased pigmentation of the yolk reserves (F), and button of the pectoral fin (G); c. mouth in ventral position (H), further development of the optic vesicle (I), and pigmentation of the heart (J); d. pigmented eyes (K) and swim bladder (L).

Fig. 4 in Ontogenetic development of tetra Astyanax lacustris (Characiformes: Characidae)

Fig. 4. Specimens of Astyanax lacustris in the postflexion stage: a. presence of food in the digestive tract (A); b. teeth (B); c. punctate pigmentation on the dorsal region of the head (C); and d. scales (D).

Fig. 6 in Ontogenetic development of tetra Astyanax lacustris (Characiformes: Characidae)

Fig. 6. Total length (mm) of Astyanax lacustris during its larval development. Arrows indicate the total consumption time of yolk reserves.

FIGURE 5 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol

FIGURE 5 | Compressed cell nucleus found in T4 of cryopreservation process of Astyanax lacustris semen.

FIGURE 1 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol

FIGURE 1 | Fluorescence photomicrograph of Astyanax lacustris (100X magnification) of nuclei with different types of damage assessed by the Comet Assay: damage 0 - no apparent damage; damage 1 - little damage; damage 2 - average damage; damage 3 - major damage.

FIGURE 4 in Effect of dietary omega-3 polyunsaturated fatty acids supplementation of Astyanax lacustris males on semen quality

FIGURE 4 | Results obtained for A. Head lateral displacement amplitude (ALH, µm) and B. Crossbeat frequency (BCF, Hz), after feeding with the inclusion of PUFAs-ô3 in the diet of Astyanax lacustris for a period of 105 days. In-0 – diet without inclusion of PUFAs-ô3; In-3 – feed with inclusion of 3% PUFAs-ô3; In-6 – feed with inclusion of 6% of PUFAs-ô3; In-9 – feed with inclusion of 9% PUFAs-ô3. Different lowercase letters indicate statistical difference between treatments for ALH and BCF (Kruskal-Wallis, p<0.05).

FIGURE 3 in Effect of dietary omega-3 polyunsaturated fatty acids supplementation of Astyanax lacustris males on semen quality

FIGURE 3 | Results obtained for A. Linearity (LIN, %), B. Straightness coefficient (STR, %) and C. Mean oscillation of the spatial trajectory (WOB, %), after feeding with the inclusion of PUFAs-ô3 in the diet of Astyanax lacustris for a period of 105 days. In-0 – diet without inclusion of PUFAs-ô3; In-3 – feed with inclusion of 3% PUFAs-ô3; In-6 – feed with inclusion of 6% of PUFAs-ô3; In-9 – feed with inclusion of 9% PUFAs-ô3. Different lowercase letters indicate statistical difference between treatments for LIN, STR and WOB (Kruskal-Wallis, p<0.05).

FIGURE 2 in Effect of dietary omega-3 polyunsaturated fatty acids supplementation of Astyanax lacustris males on semen quality

FIGURE 2 | Results obtained for A. Percentage of fast (SptzRápido), medium (SptzMedium) and slow (SptzSlow), B. Curvilinear velocity (VCL, µm/s), C. Linear velocity (VSL, µm/ s), D. Mean velocity (VAP, µm/s%) after feeding with the inclusion of PUFAs-ô3 in the diet of Astyanax lacustris for a period of 105 days. In-0 – diet without inclusion of PUFAs-ô3; In-3 – feed with inclusion of 3% PUFAs-ô3; In-6 – feed with inclusion of 6% of PUFAs-ô3; In-9 – feed with inclusion of 9% PUFAs-ô3. Different capital letters indicate statistical difference between treatments for SptzFast. Different lowercase letters indicate statistical difference between treatments for SptzMedium, SptzSlow percentage, VCL, VSL and VAP (Kruskal-Wallis, p<0.05).

FIGURE 1 in Effect of dietary omega-3 polyunsaturated fatty acids supplementation of Astyanax lacustris males on semen quality

FIGURE 1 | Results obtained for total (MOT, %) and progressive (PRG, %) sperm motility after feeding with the inclusion of PUFAs-ô3 in the diet of Astyanax lacustris for a period of 105 days. In-0 – diet without inclusion of PUFAs-ô3; In-3 – feed with inclusion of 3% PUFAs-ô3; In-6 – feed with inclusion of 6% of PUFAs-ô3; In-9 – feed with inclusion of 9% PUFAs-ô3. Different capital letters indicate statistical difference between treatments for MOT. Different lowercase letters indicate statistical difference between treatments for PRG (Kruskal-Wallis, p<0.05).

FIGURE 5 in High rDNA polymorphisms in Astyanax lacustris (Characiformes: Characidae): new insights about the cryptic diversity in A. bimaculatus species complex with emphasis on the Paraná River basin

FIGURE 5 | Barcoding Gap. Histogram generated in the ABGD showing the intraspecific variation and interspecific divergence of haplogroups 1 (green), 2 (blue) and 3 (orange).

FIGURE 3 in High rDNA polymorphisms in Astyanax lacustris (Characiformes: Characidae): new insights about the cryptic diversity in A. bimaculatus species complex with emphasis on the Paraná River basin

FIGURE 3 | Cytotypes found in Astyanax lacustris submitted to Fluorescence in situ Hybridization (FISH) with 5S (red) and 18S rDNA (green) probes. Columns represent the chromosome pair of the karyotype and lines represent the 13 cytotypes. The first column shows the first metacentric chromosome pair of the karyotype for proportion comparison.

FIGURE 4 in High rDNA polymorphisms in Astyanax lacustris (Characiformes: Characidae): new insights about the cryptic diversity in A. bimaculatus species complex with emphasis on the Paraná River basin

FIGURE 4 | A. Bayesian Inference Phylogeny; B. Haplotype data. A. Bars on the right hand side represent the Automatic Barcode Gap Discovery (ABGD), Unweighted Pair Group Method using Arithmetic averages (UPGMA), dendrogram using the Hasegawa Kishino-Yano model with gama distribution (HKY+G) and Maximum Parsimony, respectively. B. The haplotype data show the Astyanax haplogroups 1 (in green), 2 (in blue) and 3 (in orange). Haplotypes (H) 2, 3, 4, 5, 7 and 8 (in bold with an asterisk) indicate the position of one or more individuals of this study. The black slices on the haplotypes represent slightly different individuals that do not arrange another haplotype.

FIGURE 1 in High rDNA polymorphisms in Astyanax lacustris (Characiformes: Characidae): new insights about the cryptic diversity in A. bimaculatus species complex with emphasis on the Paraná River basin

FIGURE 1 | Sampling sites of the specimens (in detail). Remaining points correspond to the sequences from BOLD System. Symbols represent the haplogroups recovered here. Haplogroup 1 represent specimens of Astyanax lacustris and one A. bimaculatus (highlighted with an asterisk*). Haplogroup 2 has only A. lacustris specimens. Haplogroup 3 indicates only one A. bimaculatus specimen. Outgroup is represented by A. scabripinnis and A. cf. fasciatus. VR = Vila Rica, SS = Sub-Sede, CA = Esquina Céu Azul, SG = São Gabriel.

FIGURE 2 in High rDNA polymorphisms in Astyanax lacustris (Characiformes: Characidae): new insights about the cryptic diversity in A. bimaculatus species complex with emphasis on the Paraná River basin

FIGURE 2 | Karyotypes of Astyanax lacustris from the Sub-Sede population representing all populations analyzed in this paper. Metaphase in Giemsa with Ag-NORs in boxes.

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FIGURE 2. Astyanax lacustris, MCP 20339, 60.8 in Review of species of the Astyanax bimaculatus " caudal peduncle spot " subgroup sensu Garutti & Langeani (Characiformes, Characidae) from the rio La Plata and rio São Francisco drainages and coastal systems of southern Brazil and Uruguay

FIGURE 2. Astyanax lacustris, MCP 20339, 60.8 mm SL, Goiás, rio Corumbá; jaws and dentition, lateral view, left side.

Fig. 5 in Ontogenetic development of tetra Astyanax lacustris (Characiformes: Characidae)

Fig. 5. Survival curve, from hatching to the end of the postflexion stage, of Astyanax lacustris. Arrows indicate the most critical periods for larval survival.

Fig. 2 in Ontogenetic development of tetra Astyanax lacustris (Characiformes: Characidae)

Fig. 2. Development of Astyanax lacustris during the preflexion stage: a. optical vesicle (A); b. fins pectoral (B); c. operculum (C), gill arches (D); d. food in the digestive tract (D).

Fig. 3 in Ontogenetic development of tetra Astyanax lacustris (Characiformes: Characidae)

Fig. 3. Individual of Astyanax lacustris in the flexion stage: a. myomeres (A); b. flexion of the notochord (B).

FIGURE 4 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol

FIGURE 4 | Index of DNA damage generated by the cryopreservation process of Astyanax lacustris semen. Control - fresh semen, T1 - 10% egg yolk + 5% glucose + Me2SO10%, T2 - 10% egg yolk + 5% glucose + Me 2 SO15%, T3 - 10% egg yolk + 5% glucose + MTG010%, T5 - BTS5% + Me2 SO10%, T6 - BTS5% + Me2 SO15%, T7 - 5% Glucose + Me2 SO10% and T8 - 5% Glucose + Me2SO15%. Different letters indicate significant differences (p <0.05) by the Kruskal-Wallis test.

FIGURE 2 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol

FIGURE 2 | Comparative analysis of sperm motility of Astyanax lacustris. Total and progressive motility (A), curvilinear velocity (B), straight line velocity (C) and average path velocity (D). Different letters indicate significant differences (p <0.05) by the Scott-Knott test: Upper case relationship between control and treatments; tiny relationship between treatments.