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57 results for “Bacillus cereus”
Evaluation of MALDI‐ToF Mass Spectrometry for Rapid Detection of Cereulide from Bacillus cereus Cultures - MALDI-ToF Mass Spectra
<p>Datasets in support of the <em>bioRxiv </em>submitted paper Doellinger et al. (<strong>2019</strong>) "<em>Evaluation of MALDI‐ToF Mass Spectrometry for Rapid Detection of Cereulide from Bacillus cereus Cultures"</em> - MALDI-ToF Mass Spectra.</p> <p>The experiment and sample description and spectra numbering is consistent with the publication. Mass spectral data files are provided as unprocessed raw data in the manufacturer's original data format (Bruker Daltonics). Data is compressed using the freely available 7zip software.</p> <p><strong>Content:</strong></p> <p><em><strong>Figure 1.zip</strong></em>: Cereulide detection in <em>B. cereus</em> samples cultivated using different cultivation media and different sample preparation, or cereulide extraction methods.</p> <p><em><strong>Figure 2.zip</strong></em>: Effectivity of cereulide extraction by different solvents from <em>B. cereus</em> F4810/72 colony material.</p> <p><em><strong>Figure 3.zip</strong></em>: MALDI LIFT-ToF /ToF MS spectrum of cereulide.</p> <p><em><strong>Figure 4.zip</strong></em>: Determination of the limit of detection (LOD) of cereulide by MALDI- and LDI-ToF</p> <p><em><strong>Table 1.zip</strong></em>: Analysis of cereulide in <em>B. cereus</em> strains by MALDI-ToF MS.</p> <p><em><strong>Fig.SI.01.zip: </strong></em> Ultraperformance Liquid Chromatography – Mass Spectrometry (UPLC-MS/MS) analysis of ethanolic washing solutions of <em>B. cereus</em> F4810/72.</p> <p><em><strong>Fig.SI.02.zip:</strong></em> A selection of MALDI-ToF and LDI-ToF technical replicate mass spectra obtained from a commercial cereulide standard.</p> <p><em><strong>Fig.SI.03.zip:</strong></em> Limit of detection (LOD) of cereulide determined by MALDI- and LDI-ToF MS of ethanol wash solutions from <em>B. cereus</em> ATCC 10987 spiked by a cereulide standard. </p> <p><em><strong>Fig.SI.04.zip:</strong></em> Direct cereulide detection by means of MALDI- (panels <strong>A</strong>-<strong>F</strong>) and LDI-ToF MS (panels <strong>G</strong>-<strong>M</strong>) in linear and reflectron measurement mode.</p>
BTyperDB: a community-curated, global atlas of Bacillus cereus sensu lato genomes for epidemiological surveillance
<p>The ability to cause foodborne illness, anthrax, and other infections has been attributed to numerous lineages within <em>Bacillus cereus sensu lato</em> (<em>s.l.</em>). However, existing pathogen surveillance databases facilitate dangerous pathogen misidentifications when applied to <em>B. cereus s.l.</em>, potentially hindering outbreak or bioterrorism attack response efforts. To address this, we developed BTyperDB (<a href="http://www.btyper.app/">www.btyper.app</a>), an atlas of <em>B. cereus s.l.</em> genomes with standardized, community-curated metadata. BTyperDB aggregates all publicly available <em>B. cereus s.l.</em> genomes (including >2,600 previously unassembled genomes) with novel genomes donated by laboratories around the world, nearly doubling the number of publicly available <em>B. cereus s.l.</em> genomes. To showcase its utility for pathogen surveillance, we use BTyperDB to identify emerging anthrax toxin- and capsule-harboring lineages. Overall, our study provides insight into the epidemiology of an under-studied group of emerging pathogens and highlights the benefits of inclusive, community-driven metadata FAIRification efforts.</p>
Figure 6 in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites
Figure 6. Pyorubin production of P. aeruginosa (●), grown in NB medium under static conditions at 37 °C.
Figure 4. H O in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites
Figure 4. H O diameter of B. cereus (○) and P. aeruginosa (●), 2 2 grown in NB medium under static conditions at 37 °C.
Figure 3. Las B in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites
Figure 3. Las B activity of B.cereus (○) andP.aeruginosa (●), grown in NB medium under static conditions at 37 °C.
Figure 2 in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites
Figure 2. Biofilm levels ofB.cereus (○) and P.aeruginosa (●), grown in NB medium under static conditions at 37 °C.
Figure 1 in Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites
Figure 1. Amylase unit activity of B. cereus (○) and P. aeruginosa (●), grown in NB medium under static conditions at 37 °C.
Constraints in temperature adaptation reinforce differences in thermal niche between mesophilic and psychrotolerant Bacillus cereus group species
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Bacillus Cereus Infection of Patients Diagnosed at Nîmes University Hospital
ClinicalTrials.gov study NCT06328634. IPD Sharing: Not stated. Countries: 0. Publications: 1.
Data from: Deciphering a survival strategy during the interspecific competition between Bacillus cereus MSM-S1 and Pseudomonas sp. MSM-M1
Interspecific competition in bacteria governs colony growth dynamics and pattern formation. Here, we demonstrate an interesting phenomenon of interspecific competition between Bacillus cereus MSM-S1 and Pseudomonas sp. MSM-M1, where secretion of an inhibitor by Pseudomonas sp. is used as a strategy for survival. Although B. cereus grows faster than Pseudomonas sp., in the presence of Pseudomonas sp. the population of B. cereus reduces significantly, whereas Pseudomonas sp. do not show any marked alteration in their population growth. Appearance of a zone of inhibition between growing colonies of two species on nutrient agar prevents the expanding front of the MSM-S1 colony from accessing and depleting nutrients in the region occupied by MSM-M1, thereby aiding the survival of the slower growing MSM-M1 colonies. To support our experimental results, we present simulations, based on a chemotactic model of colony growth dynamics. We demonstrate that the chemical(s) secreted by Pseudomonas sp. is responsible for the observed inhibition of growth and spatial pattern of the B. cereus MSM-S1 colony. Our experimental results are in excellent agreement with the numerical results and confirm that secreted inhibitors enable Pseudomonas sp. to survive and coexist in the presence of faster growing B. cereus, in a common niche.
Data from: Bacillus cereus AR156 activates defense responses to Pseudomonas syringae pv. tomato in Arabidopsis thaliana similarly to flg22
Bacillus cereus AR156 (AR156) is a plant growth promoting rhizobacterium capable of inducing systemic resistance to Pseudomonas syringae pv. tomato (Pst) in Arabidopsis thaliana (Arabidopsis). Here we show that when applied to Arabidopsis leaves, AR156 acted similarly to flg22, a typical pathogen-associated molecular pattern (PAMP), in initiating PAMP-triggered immunity (PTI). AR156-elicited PTI responses included phosphorylation of MPK3 and MPK6, induction of the expression of defense-related genes PR1, FRK1, WRKY22, and WRKY29; production of reactive oxygen species; and callose deposition. Pretreatment with AR156 still significantly reduced Pst multiplication and disease severity in NahG transgenic plants and mutants sid2-2, jar1, etr1, ein2, npr1, and fls2. This suggests that AR156-induced PTI responses require neither salicylic acid, jasmonic acid, and ethylene signaling; nor flagella receptor kinase FLS2, the receptor of flg22. On the other hand, AR156 and flg22 acted in concert to differentially regulate a number of AGO1-bound miRNAs that function to mediate PTI. A full-genome transcriptional profiling analysis indicated that AR156 and flg22 activated similar transcriptional programs, co-regulating the expression of 117 genes; their concerted regulation of 16 genes was confirmed by real-time quantitative PCR analysis. These results suggest that AR156 activates basal defense responses to Pst in Arabidopsis similarly to flg22.
Data from: Pan-genome and phylogeny of Bacillus cereus sensu lato
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Data from: Bacillus cereus AR156 activates defense responses to Pseudomonas syringae pv. tomato in Arabidopsis thaliana similarly to flg22
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Data from: Deciphering a survival strategy during the interspecific competition between Bacillus cereus MSM-S1 and Pseudomonas sp. MSM-M1
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Identification of CdnL, a putative transcriptional regulator involved in repair and outgrowth of heat-damaged Bacillus cereus spores
GEO Series GSE73043. Bacillus cereus ATCC 14579. 24 samples. Type: Expression profiling by array.
Transcriptome comparison between Bacillus cereus ATCC 10987 cells with and without iron starvation
GEO Series GSE74045. Bacillus cereus ATCC 10987. 24 samples. Type: Expression profiling by array.
SigB modulates expression of novel SigB regulon members via Bc1009 in non-stressed and heat-stressed cells revealing its alternative roles in Bacillus cereus
GEO Series GSE213958. Bacillus cereus ATCC 14579. 16 samples. Type: Expression profiling by array.
Impact of pCER270 on Bacillus cereus sensu lato gene expression
GEO Series GSE220688. Bacillus cereus; Bacillus thuringiensis; Bacillus mycoides. 36 samples. Type: Expression profiling by high throughput sequencing.
Distinct roles of ComK1 and ComK2 in gene regulation in Bacillus cereus (induced ComK1 or ComK2)
GEO Series GSE27264. Bacillus cereus; Bacillus cereus ATCC 14579. 6 samples. Type: Expression profiling by array.
Multiple roles for CodY in the type strain Bacillus cereus ATCC 14579
GEO Series GSE31017. Bacillus cereus ATCC 14579. 4 samples. Type: Expression profiling by array.
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International Brain Laboratory public data
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OpenNeuro
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