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187 results for “Barcode region”
Figs 22–34 in MORPHOLOGICAL REDESCRIPTION AND DNA BARCODING OF TORLEYA PADUNICA KAZLAUSKAS, 1963 (EPHEMEROPTERA, EPHEMERELLIDAE) FROM THE EAST PALAEARCTIC REGION
Figs 22–34. Larvae of Torleya padunica. 22 – pro- and mesonotum; 23–25 – legs (23 – fore, 24 – mid, 25 – hind); 26–28 claws (26 – fore leg, 27 – mid leg, 28 – hind leg); 29 – abdomen, dorsal view; 30– gill I pair; 31–34 gills, sketchily (31 – II pair, 32 – III pair, 33 – IV pair, 34 – V pair). 9
Figs 6–10 in MORPHOLOGICAL REDESCRIPTION AND DNA BARCODING OF TORLEYA PADUNICA KAZLAUSKAS, 1963 (EPHEMEROPTERA, EPHEMERELLIDAE) FROM THE EAST PALAEARCTIC REGION
Figs 6–10. Torleya padunica: 6–11 male imago; 12 – female imago. 6 – genitalia; 7 – styliger and gonostyli; 8 – 3rd segment of gonostyli; 9 – genitalia; 10, 11 – penis; 12 – subanal plate. 6 – lateral view, 7, 10–12 – ventral view; 8, 9 – dorsal view.
Fig. 40 in MORPHOLOGICAL REDESCRIPTION AND DNA BARCODING OF TORLEYA PADUNICA KAZLAUSKAS, 1963 (EPHEMEROPTERA, EPHEMERELLIDAE) FROM THE EAST PALAEARCTIC REGION
Fig. 40. Ultrametric Bayesian inference (BI) tree based on the cytochrome c oxidase I (COI) nucleotide sequence data of the genus Torleya Lestage. Bayesian posterior probabilities (higher than 0.7) are given above tree nodes. Specimens obtained in this study are in bold.
Figs 15–21 in MORPHOLOGICAL REDESCRIPTION AND DNA BARCODING OF TORLEYA PADUNICA KAZLAUSKAS, 1963 (EPHEMEROPTERA, EPHEMERELLIDAE) FROM THE EAST PALAEARCTIC REGION
Figs 15–21. Larvae of Torleya padunica. 15 – head; 16 – labrum; 17 –hypopharynx and superlinguas; 18, 19 – mandibles, ventral view (18 – right, 19 – left); 20 – maxilla; 21 – labium.
Figs 36–39. Torleya mikhaili, genitalia. 36 in MORPHOLOGICAL REDESCRIPTION AND DNA BARCODING OF TORLEYA PADUNICA KAZLAUSKAS, 1963 (EPHEMEROPTERA, EPHEMERELLIDAE) FROM THE EAST PALAEARCTIC REGION
Figs 36–39. Torleya mikhaili, genitalia. 36 – styliger and gonostyli; 37 – penis; 38 – penis and gonostyli; 39 – penis lobes. 36, 38–39 – ventral view; 37 – dorsal view.
Fig. 6 in Phortica eparmata species complex (Diptera, Drosophilidae) from the Oriental Region, with DNA barcoding information of Chinese species
Fig. 6. Phortica (Phortica) yena sp. nov., ♂. A. Epandrium and cercus; B. Surstylus; C. Hypandrium, paramere, aedeagus and aedeagal apodeme. Scale bars = 0.1 mm.
Fig. 5 in Phortica eparmata species complex (Diptera, Drosophilidae) from the Oriental Region, with DNA barcoding information of Chinese species
Fig. 5. Phortica (Phortica) wongding sp. nov., ♂. A. Epandrium and cercus; B. Surstylus; C. Hypandrium, paramere, aedeagus and aedeagal apodeme. Scale bars = 0.1 mm.
Fig. 1 in Phortica eparmata species complex (Diptera, Drosophilidae) from the Oriental Region, with DNA barcoding information of Chinese species
Fig. 1. Bayesian tree based on the combined data of COI gene. Numbers around the node indicate the Bayesian posterior probability; results lower than 0.5 are not shown.
Fig. 4 in Phortica eparmata species complex (Diptera, Drosophilidae) from the Oriental Region, with DNA barcoding information of Chinese species
Fig. 4. Phortica (Phortica) mengda sp. nov., ♂. A. Epandrium and cercus. B. Surstylus. C. Hypandrium, paramere, aedeagus and aedeagal apodeme. Scale bars = 0.1 mm.
Fig. 3 in Phortica eparmata species complex (Diptera, Drosophilidae) from the Oriental Region, with DNA barcoding information of Chinese species
Fig. 3. Phortica (Phortica) kava sp. nov., ♂. A. Arista. B. Epandrium and cercus. C. Surstylus. D. Hypandrium, paramere, aedeagus and aedeagal apodeme. Scale bars = 0.1 mm.
Fig. 2 in Phortica eparmata species complex (Diptera, Drosophilidae) from the Oriental Region, with DNA barcoding information of Chinese species
Fig. 2. Phortica (Phortica) jadete sp. nov., ♂. A. Arista. B. Epandrium and cercus (lateral view). C. Surstylus (frontal view). D. Hypandrium, paramere, aedeagus and aedeagal apodeme (lateral views). E. Paramere (frontal view). F. Tip of aedeagal median rod (ventral view). Scale bars = 0.1 mm.
Data from: Feasibility of nuclear ribosomal region ITS1 over ITS2 in barcoding taxonomically challenging genera of subtribe Cassiinae (Fabaceae)
Premise of the Study The internal transcribed spacer (ITS) region is situated between 18S and 26S in a polycistronic rRNA precursor transcript. It had been proved to be the most commonly sequenced region across plant species to resolve phylogenetic relationships ranging from shallow to deep taxonomic levels. Despite several taxonomical revisions in Cassiinae, a stable phylogeny remains elusive at the molecular level, particularly concerning the delineation of species in the genera Cassia, Senna and Chamaecrista. This study addresses the comparative potential of ITS datasets (ITS1, ITS2 and concatenated) in resolving the underlying morphological disparity in the highly complex genera, to assess their discriminatory power as potential barcode candidates in Cassiinae. Methodology A combination of experimental data and an in-silico approach based on threshold genetic distances, sequence similarity based and hierarchical tree-based methods was performed to decipher the discriminating power of ITS datasets on 18 different species of Cassiinae complex. Lab-generated sequences were compared against those available in the GenBank using BLAST and were aligned through MUSCLE 3.8.31 and analysed in PAUP 4.0 and BEAST1.8 using parsimony ratchet, maximum likelihood and Bayesian inference (BI) methods of gene and species tree reconciliation with bootstrapping. DNA barcoding gap was realized based on the Kimura two-parameter distance model (K2P) in TaxonDNA and MEGA. Principal Findings Based on the K2P distance, significant divergences between the inter- and intra-specific genetic distances were observed, while the presence of a DNA barcoding gap was obvious. The ITS1 region efficiently identified 81.63% and 90% of species using TaxonDNA and BI methods, respectively. The PWG-distance method based on simple pairwise matching indicated the significance of ITS1 whereby highest number of variable (210) and informative sites (206) were obtained. The BI tree-based methods outperformed the similarity-based methods producing well-resolved phylogenetic trees with many nodes well supported by bootstrap analyses. Conclusion The reticulated phylogenetic hypothesis using the ITS1 region mainly supported the relationship between the species of Cassiinae established by traditional morphological methods. The ITS1 region showed a higher discrimination power and desirable characteristics as compared to ITS2 and ITS1 + 2, thereby concluding to be the locus of choice. Considering the complexity of the group and the underlying biological ambiguities, the results presented here are encouraging for developing DNA barcoding as a useful tool for resolving taxonomical challenges in corroboration with morphological framework.
Figs 1–2 in MORPHOLOGICAL REDESCRIPTION AND DNA BARCODING OF TORLEYA PADUNICA KAZLAUSKAS, 1963 (EPHEMEROPTERA, EPHEMERELLIDAE) FROM THE EAST PALAEARCTIC REGION
Figs 1–2. Male imago of Torleya padunica, habitus. 1 – lateral view; 2 – dorsal view.
Figs 3–5 in MORPHOLOGICAL REDESCRIPTION AND DNA BARCODING OF TORLEYA PADUNICA KAZLAUSKAS, 1963 (EPHEMEROPTERA, EPHEMERELLIDAE) FROM THE EAST PALAEARCTIC REGION
Figs 3–5. Male imago of Torleya padunica. 3 – fore wing; 4 – hind wing; 5 – fore leg.
Figs 13–14 in MORPHOLOGICAL REDESCRIPTION AND DNA BARCODING OF TORLEYA PADUNICA KAZLAUSKAS, 1963 (EPHEMEROPTERA, EPHEMERELLIDAE) FROM THE EAST PALAEARCTIC REGION
Figs 13–14. Larvae of Torleya padunica, habitus. 13 – dorsal view; 14 – ventral view.
Fig. 35 in MORPHOLOGICAL REDESCRIPTION AND DNA BARCODING OF TORLEYA PADUNICA KAZLAUSKAS, 1963 (EPHEMEROPTERA, EPHEMERELLIDAE) FROM THE EAST PALAEARCTIC REGION
Fig. 35. Eggs of Torleya padunica. The arrows indicate the micropyles.
Data from: Feasibility of nuclear ribosomal region ITS1 over ITS2 in barcoding taxonomically challenging genera of subtribe Cassiinae (Fabaceae)
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Multiple full-length variants of the Mitochondrial COI DNA Barcode Region are prevalent in North European Sawflies
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Data from: Taxonomy, biogeography and DNA barcodes of Geodia species (Porifera, Demospongiae, Tetractinellida) in the Atlantic boreo-arctic region
Geodia species north of 60°N in the Atlantic appeared in the literature for the first time when Bowerbank described Geodia barretti and G. macandrewii in 1858 from western Norway. Since then, a number of species have been based on material from various parts of the region: G. simplex, Isops phlegraei, I. pallida, I. sphaeroides, Synops pyriformis, G. parva, G. normani, G. atlantica, Sidonops mesotriaena (now called G. hentscheli), and G. simplicissima. In addition to these 12 nominal species, four species described from elsewhere are claimed to have been identified in material from the northeast Atlantic, namely G. nodastrella and G. cydonium (and its synonyms Cydonium muelleri and Geodia gigas). In this paper, we revise the boreo-arctic Geodia species using morphological, molecular, and biogeographical data. We notably compare northwest and northeast Atlantic specimens. Biological data (reproduction, biochemistry, microbiology, epibionts) for each species are also reviewed. Our results show that there are six valid species of boreo-arctic Atlantic Geodia while other names are synonyms or mis-identifications. Geodia barretti, G. atlantica, G. macandrewii, and G. hentscheli are well established and widely distributed. The same goes for Geodia phlegraei, but this species shows a striking geographical and bathymetric variation, which led us to recognize two species, G. phlegraei and G. parva (here resurrected). Some Geodia are arctic species (G. hentscheli, G. parva), while others are typically boreal (G. atlantica, G. barretti, G. phlegraei, G. macandrewii). No morphological differences were found between specimens from the northeast and northwest Atlantic, except for G. parva. The Folmer cytochrome oxidase subunit I (COI) fragment is unique for every species and invariable over their whole distribution range, except for G. barretti which had two haplotypes. 18S is unique for four species but cannot discriminate G. phlegraei and G. parva. Two keys to the boreo-arctic Geodia are included, one based on external morphology, the other based on spicule morphology.
FIGURE 6 in DNA barcoding and regional diversity of understudied Micropeplinae (Coleoptera: Staphylinidae) in Southwest China: phylogenetic implications and a new Micropeplus from Mount Emei
FIGURE 6. Five species of Micropeplinae from China and Vietnam. A–F: three Micropeplus species most closely related to M. jason sp. n., forming the sculptus species-group and inhabiting the Chang Shan Mountain Range (A–B), Mount Gongga (C–D) and the Tam Dao Mountain Range (E–F); G–H: Cerapeplus spp., C. sinensis from the Qin Ling Mountain Range (G) and Cerapeplus sp. from the Tam Dao Mountain Range (H). Phylogenetic position of respective specimens can be found by tracing unique specimen numbers in Fig. 2, except for Micropeplus #0882 from the Cang Shan Mountain Range (A–B), which did not amplify and was not represented in the DNA analysis.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.