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8 results for “Bartsia”
Bartsia trixago L. (BR0000015251602V)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Bartsia trixago L. (BR0000010921401)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Bartsia trixago L. (BR0000021383618)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
FIGURE 3 in Bartsia lydiae, a new species of Bartsia sect. Laxae (Orobanchaceae) from the southern Peruvian Andes with a revised key to Bartsia sect. Laxae
FIGURE 3. Distribution map of Bartsia lydiae with sites where the species was encountered marked with a star. ASTER DEM raster map provided by METI and NASA Land Processes Distributed Active Archive Center.
FIGURE 2. Bartsia lydiae. A. Flower. B. Corolla lip. C. Calyx, opened out from the ventral side. D. Bract, adaxial view. E. Anther. F in Bartsia lydiae, a new species of Bartsia sect. Laxae (Orobanchaceae) from the southern Peruvian Andes with a revised key to Bartsia sect. Laxae
FIGURE 2. Bartsia lydiae. A. Flower. B. Corolla lip. C. Calyx, opened out from the ventral side. D. Bract, adaxial view. E. Anther. F. Style. Illustration based on type specimen S.P. Sylvester 1754.
FIGURE 1 in Bartsia lydiae, a new species of Bartsia sect. Laxae (Orobanchaceae) from the southern Peruvian Andes with a revised key to Bartsia sect. Laxae
FIGURE 1. Features of Bartsia lydiae. A. Inflorescence of the type specimen, S.P. Sylvester 1754. B. Habit (type specimen, S.P. Sylvester 1754). C. Immature flowers with the corolla lip incurved or straight. The glandular indumentum on the bracts is easily noticeable, the deflexed calyx lobes are characteristic of sect. Laxae (S.P. Sylvester 1407). D. Mature flowers in the foreground showing the deflexed corolla lip (S.P. Sylvester 1649). Photographs taken by S.P. Sylvester.
Data from: A phylogenomic approach based on PCR target enrichment and high throughput sequencing: resolving the diversity within the South American species of Bartsia l. (Orobanchaceae)
Advances in high-throughput sequencing (HTS) have allowed researchers to obtain large amounts of biological sequence information at speeds and costs unimaginable only a decade ago. Phylogenetics, and the study of evolution in general, is quickly migrating towards using HTS to generate larger and more complex molecular datasets. In this paper, we present a method that utilizes microfluidic PCR and HTS to generate large amounts of sequence data suitable for phylogenetic analyses. The approach uses the Fluidigm Access Array System (Fluidigm, San Francisco, CA, USA) and two sets of PCR primers to simultaneously amplify 48 target regions across 48 samples, incorporating sample-specific barcodes and HTS adapters (2,304 unique amplicons per Access Array). The final product is a pooled set of amplicons ready to be sequenced, and thus, there is no need to construct separate, costly genomic libraries for each sample. Further, we present a bioinformatics pipeline to process the raw HTS reads to either generate consensus sequences (with or without ambiguities) for every locus in every sample or—more importantly—recover the separate alleles from heterozygous target regions in each sample. This is important because it adds allelic information that is well suited for coalescent-based phylogenetic analyses that are becoming very common in conservation and evolutionary biology. To test our approach and bioinformatics pipeline, we sequenced 576 samples across 96 target regions belonging to the South American clade of the genus Bartsia L. in the plant family Orobanchaceae. After sequencing cleanup and alignment, the experiment resulted in ~25,300bp across 486 samples for a set of 48 primer pairs targeting the plastome, and ~13,500bp for 363 samples for a set of primers targeting regions in the nuclear genome. Finally, we constructed a combined concatenated matrix from all 96 primer combinations, resulting in a combined aligned length of ~40,500bp for 349 samples.
Data from: A phylogenomic approach based on PCR target enrichment and high throughput sequencing: resolving the diversity within the South American species of Bartsia l. (Orobanchaceae)
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