Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
21
datasets available to search
ShareScore release 0.9.0
Dataset results
21 results for “Biomphalaria glabrata”
Supporting data for "The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor"
<p>Methylome of the fresh water snail <em>Biomphalaria glabrata</em>. DNA was extracted from the feet of 10 individuals of <em>B. glabrata</em> originally isolated from Brazil. These snails have been cultivated in the laboratory since 1960. Tissue were grinded at 4°C and incubated in 1 ml volume of lysis buffer (20 mM TRIS pH 8; 1 mM EDTA; 100 mM NaCl; 0.5% SDS), with 0.3 mg of proteinase K at 55°C for 1 night. Afterwards, lysate was purified with phenol-chloroform and DNA was isopropanol precipitated. The extracted DNA (around 138ng/µL) was poled in equivalent amounts and Whole Genome Bisulfite Sequencing was done by GATC-biotech (www.gatc-biotech.com). The principle of this treatment is to convert non-methylated cytosines of gDNA into deoxy-uracil, whereas methylated cytosines remain intact. WGBS was done according to the Lister protocol (sequence 2 forward strands only). The reference genome (Biomphalaria-glabrata-BB02_SCAFFOLDS_BglaB1.fa) and annotation (Biomphalaria-glabrata-BB02_BASEFEATURES_BglaB1.3.gff3) used in this project are available on VectorBase (https://www.vectorbase.org/). To align our short reads, we chose to use two specific bisulfite mapping tools, BSMAP 1.0.0 (https://code.google.com/p/bsmap/) and Bismark 0.10.2 (www.bioinformatics.babraham.ac.uk /projects/bismark/), to compare their efficiency and convenience to finally work with the more suitable one on our datasets. IGV (Interactive Genomics Viewer, https://www.broadinstitute.org/igv/) was used to visualized final alignments.<br> BSMAP performed better than Bismark and was used for downstream analyses. Without default parameters alignement efficiency for BSMAP is 47.1%, allowing for 2 mismatches increases it to 55.6%. Methylation occurs predominantly in CpGs. (C methylated in CpG context: 12.4%, C methylated in CHG context: 0.5%, C methylated in CHH context: 0.5%) The major part of CpG sites, 95.7% were unmethylated, of the remaining 4.3% of CpG sites around 3.8% had low methylation, and 0.5% were completely methylated. Methylation is of the mosaic type. Methylation is relatively low with 1.2% of total cytosines. Our analyses suggested that conserved genes and genes with stable expression are localized in high methylated regions of the genome. Finally, we see that repetitive sequences were predominantly situated in low methylated regions of <em>B. glabrata</em>. </p> <p>Wiggle files were generated for CpG pairs only.</p> <p>Produced at IHPE (http://ihpe.univ-perp.fr/)</p>
Protocol for transfection by microinjection into the eggs of the parasite vector snail Biomphalaria glabrata
<p><strong>1. Egg production</strong></p> <p>Place about 30 adult snails (10 mm diameter) into a 5.5-liter water tank. Place a piece of polystyrene of (3 x 3 cm) in each tank. There is the preferred support of <em>Biomphalaria glabrata</em> for laying its eggs. The snails are fed <em>ad libitum</em> with green lettuce leaves, they can also be fed with dry spirulina to boost reproduction. Maintain water at a temperature of 25 degrees Celsius.</p> <p><strong>2. Egg collection</strong></p> <p>Gently pick up several egg layers from the polystyrene with soft holding forceps and place the eggs into a petri dish with natural mineral water (e.g. Volvic) to prevent them from drying out.</p> <p>Start sorting the eggs under the stereoscopic microscope to choose only the gastrula stage and place them into another petri dish with natural mineral water.</p> <p> </p> <p><strong>3. Preparation of the transfection solution</strong></p> <p><strong>Material:</strong></p> <p>a. <em>in vivo</em> JetPEI transfection reagent</p> <p>b. 10% glucose solution</p> <p>c. 5% glucose solution</p> <p>d. Plasmids (dCas9-SunTag-BFP and scFv-DNMT3A-GFP)</p> <p>e. 0.2 ml microtubes</p> <p>f. P10 and P200 pipettes</p> <p>g. P10 and P200 pipette tips</p> <p>h. Permanent marker</p> <p>The glucose solution and the <em>in vivo</em> jetPEI transfection reagent are equilibrated at room temperature. </p> <p>Prepare 21 µl of each plasmid at a concentration of 78 and 88 ng / µl respectively (for a total volume of 42 µl =equals 3.5 µg of DNA) add the plasmid DNA to a 0.2 ml tube (labeled as Tube A) and mix with 21 µl of 10% glucose solution. </p> <p>In another microtube (labeled as Tube B), add 21 μl of 5% glucose solution and 1 μl of <em>in vivo</em> jetPEI. </p> <p> Prepare a third tube (labeled as Tube C) with 21 µl of 5% glucose solution and 0.5 µl of <em>in vivo</em> jetPEI to inject into embryos that will serve as controls. </p> <p>Leave the solutions at room temperature while you prepare the microinjection station. </p> <p> </p> <p> </p> <p><strong>4. Preparation of the micro-injection station</strong></p> <p><strong>Material:</strong></p> <p>a. Pre-pulled glass micropipettes (1mm diameter)</p> <p>b. Watch glass</p> <p>c. Modeling clay</p> <p>d. 35 mm and 90 mm petri dishes</p> <p>e. Mineral oil (M5904, SIGMA)</p> <p>f. Wash bottle with natural mineral water (Volvic)</p> <p>g. 0.2 ml microtubes</p> <p>h. 12-well cell culture plate</p> <p>i. Fine brush</p> <p>j. Phenol red solution</p> <p>k. Pasteur pipette or dropper</p> <p>l. Dissection forceps</p> <p>m. Soft holding forceps</p> <p>n. Snail eggs in the gastrula stage</p> <p>o. Drummond Scientific Nanoject III Programmable Nanoliter Injector</p> <p> </p> <p>Take a pre-pulled glass micropipette and cut it with a scalpel to have a ~ 0.2 mm tip slightly beveled if possible.</p> <p>Before attaching the micropipette to the programmable nanoliter injector, fill it with mineral oil. If this step is not done, the injector will not work properly. This can be done with a filling needle </p> <p>attached to a hamilton syringe of 10 microliters.</p> <p>When the micropipette is filled with oil, it must be fixed on the injector. For this it is necessary to: </p> <p>Slide the chuck and collet onto the glass micropipette, then slide the black O-ring with the seal onto the wire plunger </p> <p>With the micropipette attached to the injector, press the [EMPTY] icon until the plunger is fully extended. This step can be done with the footswitch by pressing once [EMPTY] then [STOP] and then proceeding [EMPTY] with the foot switch. A single beep is emitted when the plunger is fully extended.</p> <p>Fill the micropipette with 3 µl of the control solution or the transfection solution by placing the glass micropipette tip in a 0.2 ml tube with the solution to be injected and pressing the [FILL] icon. It is desirable to fill it at a slow rate, by pressing the [FILL] icon for a few seconds, then the [STOP] icon to allow the sample to equilibrate before pressing again the '[FILL] icon.</p> <p>Note: The piston continues to extend or retract until the [STOP] icon is pressed, or until the fully extended or fully retracted position is reached.</p> <p> </p> <p>5. Microinjection</p> <p>Place a watch glass into a 35mm petri dish and secure it on one side with modeling clay to form a slope. Use soft handling forceps to transfer an egg mass and lay it on the slope side of the watch glass so that the egg mass is in a sloping position.</p> <p>Remove excess water from the eggs with absorbent paper. Rehydrate if necessary with a fine brush to improve the visibility of the embryos. To inject the sample, return to the operating mode screen by pressing the [EXIT] icon, then select the injection mode by pressing the [INJECT] icon. Set the injection volume to 30nL and the flow rate to 20nL per second using the icons [+] and [-] respectively. Press the [INJECT] icon to inject the sample. </p> <p>Inject 30nL of the microinjection solution into each egg. Place the microinjected egg masses in a 12-well cell culture plate and note with a marker whether they were microinjected with the control solution or with the solution containing the plasmids.</p> <p>We colored the injection solution with red phenol to facilitate the visibility in this video.</p> <p><strong>Monitor the expression of the plasmids</strong></p> <p>Monitor the plasmids expression 72 h after microinjection in a contrast / fluorescent microscope or in a fluorescent stereo microscope. Then sort the fluorescent snails and perform a second micro-injection with a solution containing 10 µl of single guide RNA (at a concentration of 2ng / µl), add 0.5 µl of <em>in vivo</em> jetPEI reagent and 10 µl of 5% glucose solution. 3 days after the second microinjection, collect the hatched snails in a 1.5 ml tube containing 25 µl of lysis buffer for DNA and RNA purification.</p> <p>In this photo produced under a confocal microscope we washed a veliger larva in PBS solution, then we fixed it with 4% paraformaldehyde solution and then we placed it in a slide with two drops of the Dako fluorescence mounting medium. </p> <p>96 after the transfection we can observe the expression of the green fluorescent protein, the blue fluorescent protein and the co-localization of both proteins. </p> <p>This protocol is used to perform DNA methylation changes in a target gene. This transfection protocol can be used with other plasmids, with small interfering RNAs, or with messenger RNAs.</p> <p>Produced at IHPE (http://ihpe.univ-perp.fr)</p>
Figure 4 in Reproductive alterations of Biomphalaria glabrata (Say, 1818) infected with Angiostrongylus cantonensis (Chen, 1935) and exposed to Euphorbia milii var. hislopii latex
Figure 4. Histological sections of Biomphalaria glabrata infected with Angiostrongylus cantonensis and/or exposed to Euphorbia milii var. hislopii latex: A. Control-40X - albumen gland without deformation; B. Exposed-20X - albumen gland without deformation; C. Infected-1 day-40X - albumen gland without deformation; D. Infected+Exposed-1 day-40X - albumen gland without deformation; E. Infected-7 days-20X - Cellular infiltrate with granuloma-like formation (g), with evidence of larval profiles (l), no collagen present; and F. Infected+Exposed-7 days-20X - Cellular infiltrate with granuloma-like formation (g), with evidence of larval profile (l). E stained with Masson's trichrome, the others with hematoxylin and eosin.
Figure 5 in Reproductive alterations of Biomphalaria glabrata (Say, 1818) infected with Angiostrongylus cantonensis (Chen, 1935) and exposed to Euphorbia milii var. hislopii latex
Figure 5. Histological sections of Biomphalaria glabrata infected with Angiostrongylus cantonensis and/or exposed to Euphorbia milii var. hislopii latex: A-B. With evidence of larval profile (l). A. Infected-14 days-40X - Cellular infiltrate with the granuloma-like formation (g), without the presence of collagen; B. Infected+Exposed-14 days-20X - With evidence of larval profile: C-shaped (arrow), with horizontal view and another with vertical view, presence of cellular infiltrate, with granuloma-like formation (g); C. Infected-21 days-20X - Cellular infiltrate with the granuloma-like formation (g), without the presence of collagen; D. Infected+Exposed-21 days-20X - Evidence of larval profile (l); E. Infected-28 days-40X - Cellular infiltrate with granuloma-like formation, no collagen present; and F. Infected+Exposed-28 days-20X - Evidence of larval profile (l) in the connective tissue between albumen gland acini (ag), with surrounding granuloma-like formation (g). B and F stained with hematoxylin and eosin, the others with Masson's trichrome.
Figure 3 in Reproductive alterations of Biomphalaria glabrata (Say, 1818) infected with Angiostrongylus cantonensis (Chen, 1935) and exposed to Euphorbia milii var. hislopii latex
Figure 3. Histological sections of the gonadal region of Biomphalaria glabrata infected with Angiostrongylus cantonensis and/or exposed to Euphorbia milii var. hislopii latex: A. Infected-14 days-20X - Normal looking gland with acini (ac) and formation of spermatozoa (s) and oocytes in different stages; B. Infected+Exposed-14 days-40X - Normal looking gland, acini (ac) with oocyte formation in different stages; C. Infected-21 days-20X - Normal gland, with spermatozoa (s) and granuloma-like structure formation (g) around larval profile (l); D. Infected+Exposed-21 days-20X - Normal gland, granuloma-like formation around larva (arrow); E.Infected-28 days-20X - Normal gland, granuloma-like formation (g) around larval profile (l); and F- Infected+Exposed-28 days-20X - Normal gland, granuloma-like formation (g). A-F stained with hematoxylin and eosin.
Figure 1 in Reproductive alterations of Biomphalaria glabrata (Say, 1818) infected with Angiostrongylus cantonensis (Chen, 1935) and exposed to Euphorbia milii var. hislopii latex
Figure 1. Effects of Angiostrongylus cantonensis infection and/or exposure to Euphorbia milii var. hislopii latex on the survival (%) of Biomphalaria glabrata in groups C, E, I, I+E-1D, I+E-7D, I+E-14D, I+E-21D and I+E-28D after 1, 2, 3, and 4 weeks.
Figure 2 in Shell geometric morphometrics in Biomphalaria glabrata (Mollusca: Planorbidae) uninfected and infected with Schistosoma mansoni
Figure 2. Principal component analysis diagram of the first two principal components (within percentage explained variance contribution) from 60 Biomphalaria glabrata specimens, uninfected (black dots) and infected with Schistosoma mansoni (gray dots). Ellipse encloses 90% of data for each group.
Figure 1 in Shell geometric morphometrics in Biomphalaria glabrata (Mollusca: Planorbidae) uninfected and infected with Schistosoma mansoni
Figure 1. Shell of Biomphalaria glabrata showing the landmarks (LM1–LM12) disposition. White landmarks correspond to type I and II landmarks, while the gray to semilandmarks.
Figure 3 in Shell geometric morphometrics in Biomphalaria glabrata (Mollusca: Planorbidae) uninfected and infected with Schistosoma mansoni
Figure 3. Grid deformation showing differences in the Principal Component 1 (PC1) between the mean configuration of Biomphalaria glabrata uninfected and infected with Schistosome mansoni.
Manipulations pour la micro-injection chez Biomphalaria glabrata
<ol> <li><strong>Production des œufs </strong></li> </ol> <p>Mettre dans des aquariums de 5.5L une trentaine d'escargots adultes (10 mm). Mettre un pondoir (morceau de polystyrène de 3 x 3 cm) dans chaque aquarium, c’est le substrat préféré pour faire pondre <em>Biomphalaria glabrata</em>. Les escargots sont nourris <em>ad libitum</em> avec de la salade (des feuilles laitue sans côtes), ils peuvent aussi être nourris avec de la spiruline sèche pour booster la reproduction. Maintenir les aquariums à une température de 25 degrés.</p> <ol> <li><strong>Collection d’œufs </strong></li> </ol> <p>Prenez délicatement plusieurs pontes d’œufs déposées sur les polystyrènes avec des pinces souples et placez les œufs dans une boîte de pétri avec de l’eau minérale naturelle (Volvic de préference) afin d’éviter qu’ils ne sèchent. </p> <p>Commencez à trier les œufs sous la loupe binoculaire pour choisir seulement le stade gastrula et placez-les dans une autre boîte de pétri avec de l’eau minérale naturelle. </p> <p> </p> <ol> <li><strong><em>Préparation de la solution de transfection</em></strong></li> </ol> <p><strong>Matériel :</strong></p> <ol> <li>Réactif de transfection<em> in vivo </em>jetPEI</li> <li>Solution de glucose 10%</li> <li>Solution de glucose 5% </li> <li>Plasmides (dCas9-SunTag-BFP et scFv-DNMT3A-GFP) </li> <li>Microtubes de 0.2 ml</li> <li>Pipettes P10 et P200</li> <li>Pointes de pipettes P10 et P200</li> <li>Marqueur permanent</li> </ol> <p>La solution de glucose et le réactif de transfection <em>in vivo jetPEI</em> sont équilibrés à température ambiante. Préparez 21 µl de chaque plasmide (dCas9-SunTag-BFP et scFv-DNMT3A-GFP) à une concentration de 78.8 et 88.8 ng/µl respectivement (pour un volume total de 42 µl = 3.5 µg d’ADN), ajoutez l’ensemble à un tube de 0.2ml avec 21 µl de solution de glucose à 10% (étiqueté Tube A). </p> <p> </p> <p>Dans un autre tube de 0.2ml (étiqueté Tube B), 21 µl de solution de glucose à 5% et 1 µl de <em>in vivo jetPEI</em> sont ajoutés. Préparez un autre tube (étiqueté Tube C) avec 21 µl de solution glucose à 5% et 0.5 µl de <em>in vivo jetPEI</em> pour injecter dans des embryons qui serviront de témoins. Laissez les solutions à température ambiante pendant que vous préparez le poste de micro-injection.</p> <p> </p> <ol> <li><strong>Préparation du poste de micro-injection</strong></li> </ol> <p> </p> <p><strong>Matériel :</strong></p> <ol> <li>Micropipettes en verre de 1 mm de diamètre étirées</li> <li>Verre de montre</li> <li>Pâte à modeler</li> <li>Boîtes de pétri 35 mm et 90 mm</li> <li>Huile minérale (M5904, SIGMA)</li> <li>Pissette avec de l’eau minérale naturelle (Volvic)</li> <li>Microtubes de 0.2 ml</li> <li>Plaque de culture cellulaire 12 puits</li> <li>Pinceau fin</li> <li>Solution de rouge de phénol</li> <li>Pipette Pasteur</li> <li>Forceps à dissection</li> <li>Pinces souples</li> <li>Pontes d’œufs d’escargots au stade gastrula</li> <li>Injecteur de nanolitre programmable Drummond Scientific Nanoject III</li> </ol> <p>Prenez une micropipette préalablement étirée et coupez-la avec un scalpel pour obtenir une pointe d’environ ~0.2 mm légèrement biseautée si possible.</p> <p>Avant de fixer la micropipette à l’injecteur de nanolitre programmable, la remplir d’huile minérale, sans huile minérale à l’intérieur, elle ne fonctionnera pas correctement. Cela peut être réalisé avec une aiguille de remplissage et une seringue hamilton de 10 µl.</p> <p>Lorsque la micropipette est remplie d’huile il faut la fixer sur l’injecteur, pour cela il faut glisser le mandrin et la pince de serrage sur la micropipette en verre étirée, glisser ensuite le joint d’étanchéité le long du piston, puis le positionner.</p> <p>Une fois la micropipette fixée sur l’injecteur, appuyer sur l’icône [EMPTY] jusqu’à ce que le piston soit complètement allongé, cette étape peut être effectuée avec l’interrupteur à pédale en appuyant une fois sur [EMPTY] puis sur [STOP] puis en procédant à vide avec l’interrupteur à pédale. Un seul bip est émis lorsque le piston est complètement allongé. </p> <p>Remplir la micropipette avec 3 µl de la solution contrôle ou la solution contenant les plasmides (c’est-à-dire la solution de transfection) en plaçant la pointe de la micropipette en verre dans un tube de 0.2 ml avec la solution à injecter et en appuyant sur l’icône [FILL]. Il est souhaitable de la remplir à un débit lent, en appuyant sur l’icône [FILL] pendant quelques secondes, puis sur l’icône [STOP] pour permettre à l’échantillon de s’équilibrer avant d’appuyer de nouveau sur l’icône [FILL]. </p> <p>Remarque : le piston continue de s’allonger ou de se rétracter jusqu’à ce que l’on appuie sur l’icône [STOP], ou jusqu’à ce que la position pleinement allongée ou pleinement rétractée soit atteinte. </p> <p> </p> <ol> <li><strong>Microinjection</strong></li> </ol> <p>Placez un verre de montre dans une boîte de pétri de 35 mm et fixez le d’un côté avec de la pâte à modeler pour forme une pente. Utilisez une pince souple pour transférer une masse d’œufs et posez-la sur le côté de la pente du verre de montre pour que la masse d’œufs soit dans une position inclinée. </p> <p>Enlevez l'excédent d'eau de l'œuf avec du papier absorbant contre le côté opposé aux œufs. Réhydratez si nécessaire avec un pinceau fin pour améliorer la visibilité des embryons. Pour injecter l’échantillon, retourner à l’écran du mode de fonctionnement en appuyant sur l’icône [EXIT], puis sélectionner le mode d’injection en appuyant sur l’icône [INJECT]. </p> <p>Régler le volume d’injection à 30nL et le débit à 20 nL par seconde en utilisant les icônes [+] et [-] respectivement. Appuyez sur l’icône [INJECT] pour injecter l’échantillon.</p> <p>Injectez 30nL de la solution de micro-injection dans chaque œuf. Placez les masses d’œufs micro-injectés dans une plaque de culture cellulaire de 12 puits et notez avec un marqueur s’ils ont été micro-injectés avec la solution de contrôle ou avec la solution contenant les plasmides. </p> <p>Notez que nous avons coloré avec du rouge phénol la solution d’injection pour faciliter la visibilité dans cette vidéo. </p> <p><strong>Monitorer l’expression des plasmides </strong></p> <p>Monitorez l’expression des plasmides 72 h après la micro-injection dans un microscope de contraste/fluorescente ou une loupe binoculaire fluorescente. Puis triez les escargots fluorescents et réalisez une deuxième micro-injection avec une solution contenant 10 µl d’ARN guide (2ng/µl), ainsi que 0.5 µl de réactif <em>in vivo jetPEI </em>et 10 µl de glucose 5%. 3 jours après la deuxième microinjection, récupérez les escargots éclos dans des tubes 1.5 ml contenant 25 µl du tampon de lyse pour une purification d’ADN et ARN.</p> <p>Pour cette photo élaborée au microscope confocal nous avons lavé une larve véligère dans une solution du PBS puis nous l’avons fixé avec une solution du paraformaldéhyde à 4% et ensuite nous l’avons mis dans une lame avec deux gouttes du milieu de montage de fluorescence Dako.</p> <p>96 heures après la transfection on peut observer l’expression de la protéine verte fluorescente, de la protéine bleue fluorescente et la co-localization des deux protéines. </p> <p>Ce protocole sert à effectuer des modifications de la méthylation de l’ADN dans un gène cible. Ce protocole de transfection peut être utiliser avec d’autres plasmides, avec des petits ARN interférents ou avec des ARN messagers. </p> <p>Produit de IHPE (http://ihpe.univ-perp.fr).</p>
Script and single cell RNA sequencing datasets of Biomphalaria glabrata hemocyte
<p>The results of the gene and cell barcode counts (feature-barcode matrices) are available in the file "filtered_feature_bc_matrix_Naive". These data have been processed by cellRanger v3.1.0 and can be used with the script "scRNAseq_Biomphalaria_naive" which gathers all the analyses done for publication.</p> <p> </p>
Figure 2 in Reproductive alterations of Biomphalaria glabrata (Say, 1818) infected with Angiostrongylus cantonensis (Chen, 1935) and exposed to Euphorbia milii var. hislopii latex
Figure 2. Histological sections of the gonadal region of Biomphalaria glabrata infected with Angiostrongylus cantonensis and/or exposed to Euphorbia milii var. hislopii latex: A. Control group-20X - Showing normal aspect of the gonad, with acini (ac) and occurrence of gametogenesis; B. Exposed-40X - Normal gland, with presence of spermatogenesis (s) and oogenesis, in the acini (ac); C. Infected-1 day-20X - Normal looking gland, with acini (ac), sperm formation and oocytes at different stages; D. Infected+Exposed-1 day-40X - Normal looking gland, with acini with oogenesis (ov); E. Infected-7 days-20X – Normal gland, with acini (ac) and gametogenesis; and F. Infected+Exposed-7 days-20X – Normal gland, with acini (ac) and gametogenesis at different stages. A-F stained with hematoxylin and eosin.
Altered Gene Expression in the Schistosome- Transmitting Snail Biomphalaria glabrata following Exposure to Niclosamide, the Active Ingredient in the Widely Used Molluscicide Bayluscide
GEO Series GSE71223. Biomphalaria glabrata. 16 samples. Type: Expression profiling by array.
Pathogen-associated molecular patterns activate expression of genes involved in cell proliferation, immunity and detoxification in the amebocyte-producing organ of the snail Biomphalaria glabrata
GEO Series GSE71607. Biomphalaria glabrata. 16 samples. Type: Expression profiling by array.
BS-90 strain resistance in M-line Biomphalaria glabrata
GEO Series GSE21881. Biomphalaria glabrata. 45 samples. Type: Expression profiling by array.
Acquired resistance in M-line Biomphalaria glabrata vs. Sensitized (exposed to irradiated only) and Infected (exposed to viable only) E. paraensei
GEO Series GSE21878. Biomphalaria glabrata. 48 samples. Type: Expression profiling by array.
Size resistance in M-line Biomphalaria glabrata
GEO Series GSE21880. Biomphalaria glabrata. 33 samples. Type: Expression profiling by array.
Fig. 1 Biomphalaria glabrata genomic scaffold ASM4573v1 in ELAV Intron 8: a single-copy sequence marker for shallow to deep phylogeny in Eupulmonata Hasprunar & Huber, 1990 and Hygrophila Férussac, 1822 (Gastropoda: Mollusca)
Fig. 1 Biomphalaria glabrata genomic scaffold ASM4573v1 LG4_Random_Scaffold46, whole genome shotgun sequence, with the location of Exons 8 and 9, Intron 8, and primers used for ELAVI8 PCR amplification
Transcriptional response of trematode-infected Biomphalaria glabrata during development of S. mansoni and E. paraensei
GEO Series GSE18705. Biomphalaria glabrata. 49 samples. Type: Expression profiling by array.
Differential transcriptomic responses of Biomphalaria glabrata to bacteria and metazoan parasites
GEO Series GSE16596. Biomphalaria glabrata. 21 samples. Type: Expression profiling by array.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.