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96 results for “Burkholderia”
Multiwavelength anomalous dispersion (MAD) diffraction images of Burkholderia pseudomallei BipD.
<p>Selenomethionine MAD diffraction images from beamline BM16 at the European Synchrotron Radiation Facility (ESRF, Grenoble, France) that were used for structure analysis of BipD from <em>Burkholderia pseudomallei</em>. Details of the data collection are given in the included scanned notes. </p>
Tobramycin Inhalation Powder (TIP) in Cystic Fibrosis Patients Infected With Burkholderia Cepacia Complex
ClinicalTrials.gov study NCT02212587. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Data from: Genetic architecture constrains exploitation of siderophore cooperation in the bacterium Burkholderia cenocepacia.
Abstract. Explaining how cooperation can persist in the presence of cheaters, exploiting the cooperative acts, is a challenge for evolutionary biology. Microbial systems have proved extremely useful to test evolutionary theory and identify mechanisms maintaining cooperation. One of the most widely studied system is the secretion and sharing of iron-scavenging siderophores by Pseudomonas bacteria, with many insights gained from this system now being considered as hallmarks of bacterial cooperation. Here, we introduce siderophore secretion by the bacterium Burkholderia cenocepacia H111 as a novel parallel study system, and show that this system behaves differently. For ornibactin, the main siderophore of this species, we discovered a novel mechanism of how cheating can be prevented. Particularly, we found that secreted ornibactin cannot be exploited by ornibactin-defective mutants because ornibactin receptor and synthesis genes are co-expressed from the same operon, such that disruptive mutations in synthesis genes compromise receptor availability required for siderophore uptake and cheating. For pyochelin, the secondary siderophore of this species, we found that cheating was possible, but the relative success of cheaters was positive frequency-dependent, thus diametrically opposite to the Pseudomonas and other microbial systems. Altogether, our results highlight that expanding our repertoire of microbial study systems leads to new discoveries and suggest that there is an enormous diversity of social interactions out there in nature, and we might have only looked at the tip of the iceberg so far.
Custom MALDI-TOF Mass Spectrometric Database for identification of environmental Burkholderia and related genera
<p class="CxSpFirst">Success of microbial natural products discovery programs is dependent on quick and concise discrimination between isolates from diverse environments. However, laboratory isolation and identification of priority genera using current 16S rRNA PCR-based methods is both challenging and time-consuming. In clinical settings, MALDI-TOF MS is used to rapidly identify unknown bacterial pathogens, yet this has not been widely adapted for isolation of environmental bacteria. To expedite isolation of Gram-negative proteobacterial order Burkholderiales, we have created a main spectral (MSP) library for the Bruker Biotyper MALDI mass spectrometer that includes 95 entries of <i>Burkholderia, Caballeronia, Paraburkholderia,</i> and other environmentally related genera. We tested the effectiveness of the reference library by analyzing 49 unknown environmental isolate strains before comparing to results of traditional 16S sequencing. The MALDI Biotyper correctly identified all strains to the genus level when sufficient reference spectra were available. This dataset is freely available to the scientific research community for use in taxonomic assignments of environmentally-derived bacteria from the order Burkholderiales.</p>
Protein with negative surface charge distribution, Bnr1, shows characteristics of a DNA-mimic protein and may be involved in the adaptation of Burkholderia cenocepacia
<p><strong>Supplementary tables for the publication: 'Protein with negative surface charge distribution, Bnr1, shows characteristics of a DNA-mimic protein and may be involved in the adaptation of <em>Burkholderia cenocepacia</em></strong></p> <p>Table S4: All proteins showing altered abundance by > 1.5 fold in the ∆BCAS0292 mutant strain relative to the K56-2 wild type strain in all biological replicates. </p> <p>Table S5: Comparison of the lxa-encoded proteins that showed reduced abundance in the ∆BCAS0292 mutant strain relative to the K56-2 strain and which previously showed increased expression in clincal chronic infetion</p> <p>Table S6: Comparison of all proteins that showed reduced abundance in the ∆BCAS0292 strain relative to the K56-2 wild type strain and previously showed increased abundance in late relative to early B. cenocepacia</p> <p>Table S7: Immunoprecipitation analysis raw proteome data. </p>
Identification of Environmental Determinants Involved in the Distribution of Burkholderia pseudomallei in Southeast Asia using MaxEnt software
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A Study of OligoG in Cystic Fibrosis Subjects With Burkholderia Spp. Infection
ClinicalTrials.gov study NCT02453789. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Data from: Genetic architecture constrains exploitation of siderophore cooperation in the bacterium Burkholderia cenocepacia.
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Custom MALDI-TOF Mass Spectrometric Database for identification of environmental Burkholderia and related genera
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Data from: Insecticide applications to soil contribute to development of Burkholderia mediating insecticide resistance in stinkbugs
Some soil Burkholderia strains are capable of degrading the organophosphorus insecticide, fenitrothion, and establish symbiosis with stinkbugs, making the host insects fenitrothion-resistant. However, the ecology of the symbiotic degrading Burkholderia adapting to fenitrothion in the free-living environment is unknown. We hypothesized that fenitrothion applications affect the dynamics of fenitrothion-degrading Burkholderia, thereby controlling the transmission of symbiotic degrading Burkholderia from the soil to stinkbugs. We investigated changes in the density and diversity of culturable Burkholderia (i.e. symbiotic and nonsymbiotic fenitrothion degraders and nondegraders) in fenitrothion-treated soil using microcosms. During the incubation with five applications of pesticide, the density of the degraders increased from less than the detection limit to around 106/g of soil. The number of dominant species among the degraders declined with the increasing density of degraders; eventually, one species predominated. This process can be explained according to the competitive exclusion principle using Vmax and Km values for fenitrothion metabolism by the degraders. We performed a phylogenetic analysis of representative strains isolated from the microcosms and evaluated their ability to establish symbiosis with the stinkbug Riptortus pedestris. The strains that established symbiosis with R. pedestris were assigned to a cluster including symbionts commonly isolated from stinkbugs. The strains outside the cluster could not necessarily associate with the host. The degraders in the cluster predominated during the initial phase of degrader dynamics in the soil. Therefore, only a few applications of fenitrothion could allow symbiotic degraders to associate with their hosts and may cause the emergence of symbiont-mediated insecticide resistance.
Data from: Burkholderia bacteria infectiously induce the proto-farming symbiosis of Dictyostelium amoebae and food bacteria
Symbiotic associations can allow an organism to acquire novel traits by accessing the genetic repertoire of its partner. In the Dictyostelium discoideum farming symbiosis, certain amoebas (termed "farmers") stably associate with bacterial partners. Farmers can suffer a reproductive cost but also gain beneficial capabilities, such as carriage of bacterial food (proto-farming) and defense against competitors. Farming status previously has been attributed to amoeba genotype, but the role of bacterial partners in its induction has not been examined. Here, we explore the role of bacterial associates in the initiation, maintenance, and phenotypic effects of the farming symbiosis. We demonstrate that two clades of farmer-associated Burkholderia isolates colonize D. discoideum nonfarmers and infectiously endow them with farmer-like characteristics, indicating that Burkholderia symbionts are a major driver of the farming phenomenon. Under food-rich conditions, Burkholderia-colonized amoebas produce fewer spores than uncolonized counterparts, with the severity of this reduction being dependent on the Burkholderia colonizer. However, the induction of food carriage by Burkholderia colonization may be considered a conditionally adaptive trait because it can confer an advantage to the amoeba host when grown in food-limiting conditions. We observed Burkholderia inside and outside colonized D. discoideum spores after fruiting body formation; this observation, together with the ability of Burkholderia to colonize new amoebas, suggests a mixed mode of symbiont transmission. These results change our understanding of the D. discoideum farming symbiosis by establishing that the bacterial partner, Burkholderia, is an important causative agent of the farming phenomenon.
Safety and Efficacy Study of Aztreonam for Inhalation Solution (AZLI) in Patients With Cystic Fibrosis and Chronic Burkholderia Species Infection
ClinicalTrials.gov study NCT01059565. IPD Sharing: Not stated. Countries: 2. Publications: 0.
Data from: Insecticide applications to soil contribute to development of Burkholderia mediating insecticide resistance in stinkbugs
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Data from: The specificity of Burkholderia symbionts in the social amoeba farming symbiosis: prevalence, species, genetic and phenotypic diversity
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Data from: Burkholderia bacteria infectiously induce the proto-farming symbiosis of Dictyostelium amoebae and food bacteria
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Data from: Live imaging of symbiosis: spatiotemporal infection dynamics of GFP-labelled Burkholderia symbiont in the bean bug Riptortus pedestris
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The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111
GEO Series GSE41244. Burkholderia cenocepacia H111. 3 samples. Type: Expression profiling by high throughput sequencing.
NtrC-dependent control of exopolysaccharide synthesis and motility in Burkholderia cenocepacia H111
GEO Series GSE95607. Burkholderia cenocepacia H111. 6 samples. Type: Expression profiling by high throughput sequencing.
Identification of strong constitutive promoters in Burkholderia to activate natural product production in Gram-negative bacteria
GEO Series GSE269653. Burkholderia stagnalis. 18 samples. Type: Expression profiling by high throughput sequencing.
Burkholderia pseudomallei transcriptional adaptation in macrophage
GEO Series GSE27558. Burkholderia pseudomallei K96243; Burkholderia pseudomallei. 15 samples. Type: Expression profiling by array.
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