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50 results for “Bursaphelenchus”

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zenodo40/100

Study of the non-vector spread of the pine nematode Bursaphelenchus xylophilus through sawdust

<p>The goal of the study was to assess the possibility of <em>Pinus sylvestris</em> trees to be infested with pinewood nematode <em>B. xylophilus</em> through PWN-infested sawdust. The trials were conducted in climatic room at a temperature of 26℃ and at humidity of 60-70% from June to October, 2020. Each trial included 4-year-old seedlings of 12 <em>Pinus sylvestris </em>pines.</p> <p>The experiment contained seven trials, including the control: 1) uninjured stem + PWN-infested sawdust, 2) injured stem + PWN-infested sawdust, 3) injured stem + PWN-infested sawdust is 2.5 cm from the stem, 4) uninjured roots + PWN-infested sawdust in soil, 5) injured roots + PWN-infested sawdust in soil, 6) injured roots without sawdust, and 7) control. At the end of the experiment, the number of pinewood nematodes in the stem and roots of infested seedlings was counted. The number of nematodes is based on 100 grams wet weight of woody substrate and 100 cm<sup>3</sup> for soil substrate. We counted the number of wilted pine seedlings taking into account wilt classes (from 0 to 5) in different trials at the 20th week of the experiment.&nbsp;</p>

opencc-by-4.0Oct 2022View details →
zenodo40/100

Figure A1 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure A1: Principal components analysis of variation (A) and correlation coefficient analysis (B) among sequenced transcriptomes to show correlation among samples (control, CK1-3 and the treated samples, P1-3).

opencc-by-4.0Mar 2020View details →
zenodo40/100

Figure A3 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure A3: Observation of the normal PWNs (A) and punicalagin-treated PWNs twisting abnormally (B) under microscope.

opencc-by-4.0Mar 2020View details →
zenodo40/100

Figure 5 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure 5: Expression of six differentially expressed genes by (A) RNA-Seq, (B) qRT-PCR and (C) their correlation.

opencc-by-4.0Mar 2020View details →
zenodo40/100

Figure A2 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure A2: Annotated KEGG pathway of Endocytosis (A), Peroxisome (B) and MAPK signaling pathways (C) about differentially expressed genes. Genes in blue frames with red borders were up-regulated, genes in blue frames with yellow borders were down-regulated and genes in blue frames with sky-blue borders were simultaneously up-regulated and down-regulated.

opencc-by-4.0Mar 2020View details →
zenodo40/100

Figure 4 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure 4: Annotation of differentially expressed genes (DEGs). (A) Column diagram of DEGs using GO annotation. The bottom X-axis indicates the number of genes annotated on different GO terms. The X-axis indicates the ratios of genes annotated on different GO terms to all terms used for the GO annotation. (B) Scatter diagram of DEGs with GO enrichment. (C) KOG functional classification of DEGs.

opencc-by-4.0Mar 2020View details →
zenodo40/100

Figure 3 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure 3: Functional annotation of unigenes. (A) GO functional annotation statistics on level 2. (B) KOG annotation statistics. (C) KEGG pathway annotation statistics. A. Cellular processes. B. Environmental information processing. C. Genetic information processing. D. metabolism. E. Organismal systems.

opencc-by-4.0Mar 2020View details →
zenodo40/100

Figure 1 in First report of Bursaphelenchus fungivorus (Nematoda: Aphelenchida) in Italy and an overview of nematodes associated with Crocus sativus L.

Figure 1: Light micrographs of BUrSaphelenChUS fUngiVOrUS. A: Male total body; B: Female anterior region; C: Female tail; D: Vulval region; E: Lateral view of male tail; F: Ventral view of male tail (Scale bar A = 100 µm; B-F = 20µ m).

opencc-by-4.0Mar 2020View details →
zenodo40/100

Figure 2 in First report of Bursaphelenchus fungivorus (Nematoda: Aphelenchida) in Italy and an overview of nematodes associated with Crocus sativus L.

Figure 2: Phylogenetic relationships of species belonging to fUngiVOrUS-group based on ITS locus. The tree was inferred using GTR + I + G as nucleotide substitution model, tested with 1,000 bootstrap replicates and BUrSaphelenChUS COCOphilUS was chosen as outgroup species. Nodes were annotated with NJ and ML bootstrap values, respectively.

opencc-by-4.0Mar 2020View details →
zenodo36/100

Figure 4 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure 4: (CONtiNUed)

opencc-by-4.0Mar 2020View details →
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Figure 2 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure 2: Functional annotation statistics of unigenes.

opencc-by-4.0Mar 2020View details →
zenodo36/100

Figure 1 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure 1: Sequence length distribution of assembled unigenes.

opencc-by-4.0Mar 2020View details →
zenodo36/100

Figure A2 in Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Figure A2: (CONtiNUed)

opencc-by-4.0Mar 2020View details →
zenodo36/100

Bursaphelenchus xylophilus ̶ Pest Report and Datasheet to support ranking of EU candidate priority pests

<p>These two files are part of the outputs produced under the mandate&nbsp;<a href="http://registerofquestions.efsa.europa.eu/roqFrontend/wicket/page?1-1.ILinkListener-contentPane-listContainer-pageable-21-mandateNumberLnk">M-2017-0056</a>&nbsp;of the European Commission requesting EFSA for technical assistance in the field of quarantine pests qualifying as priority pests as by Article 6(2) of the Regulation (EU) 2016/2031 <em>on protective measures against pests of plants</em>.</p> <p>Under the mandate EFSA produced: i) 1 methodology report (DOI available at the field &quot;Related/alternate identifiers&quot;), ii) 28 datasheets, one for each of the 28 candidate pests, and iii) 28 pest reports supporting the information provided in the datasheets.</p> <p>EFSA wishes to acknowledge the contribution of Hugh Evans and Christer Magnusson to the EKE and the review conducted by Gregor Urek.</p>

opencc-by-4.0Jun 2019View details →
zenodo32/100

FIGURES 1­14 in Redescription of Bursaphelenchus talonus (Thorne, 1935) Massey, 1956 (Nematoda: Parasitaphelenchidae) and designation of lectotypes

FIGURES 1­14. Bursaphelenchus talonus (Thorne, 1935) Massey, 1956. 1, full view of male; 2, full view of female; 3, head and neck of male; 4, male tail; 5, male tail showing bursa; 6, lateral field; 7, variation in female tails; 8, vulval region; 9, head and neck; 10, female tail; 11, male tail; 12, spicules; 13, stylet; 14, variation in spicule shape. Note: 9­12 are redrawn from Thorne, 1935.

opennotspecifiedAug 2003View details →
zenodo32/100

FIGURE 2 in Supplementary characters of Bursaphelenchus lini Braasch, 2004 (Nematoda: Parasitaphelenchidae) and remarks on this nematode

FIGURE 2. ITS­RFLP patterns obtained from two individual specimens of Bursaphelenchus lini (left: type A, right: type B). Restriction fragments were obtained by digestion of the amplified rDNA fragment (0) with Rsa I (1), Hae III (2), Msp I (3), Hinf I (4) and Alu I (5). M: DNA size marker (100 bp ladder; Invitrogen Life Technologies).

opennotspecifiedMar 2006View details →
zenodo32/100

FIGURE 1 in Supplementary characters of Bursaphelenchus lini Braasch, 2004 (Nematoda: Parasitaphelenchidae) and remarks on this nematode

FIGURE 1. Scanning electron micrographs of Bursaphelenchus lini: A. Head region, lateral view; B. Enface view of female head; C. Lateral field; D. Female tail; E. Vulval region; F.­I: Male tail showing papillae (F, G), bursa (H) and protracted spicules (I).

opennotspecifiedMar 2006View details →
zenodo32/100

FIGURE 2 in Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group

FIGURE 2. Light photomicrographs of Bursaphelenchus singaporensis sp. n. A, B: Head region; C, D: Female tail; E, F: Male bursa; G, H: Male tail; I: Vulval region. (Scale bars = 10 µm).

opennotspecifiedMay 2005View details →
zenodo32/100

FIGURE 1 in Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group

FIGURE 1. Bursaphelenchus singaporensis sp. n. A: Female; B: Male; C: Head region; D: Female tails; E: Male tail; F: Vulval region; G: Bursa; H:. Spicules. (Scale bars = 10 µm).

opennotspecifiedMay 2005View details →
zenodo32/100

FIGURE 4 in Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore-a new species of the B. xylophilus group

FIGURE 4. ITS­RFLP patterns of Bursaphelenchus singaporensis sp. n. and six morphologically similar species. Each picture shows (from left to right) DNA marker (100 bp ladder, Invitrogen), rDNA amplification product, restriction fragments obtained with Rsa I, Hae III, Msp I, Hinf I, Alu I, and again DNA marker.

opennotspecifiedMay 2005View details →

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