Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
82
datasets available to search
ShareScore release 0.9.0
Dataset results
82 results for “CAG”
Data from: Suppression of Huntington’s disease somatic instability by transcriptional repression and direct CAG repeat binding
Open the record for dataset details and reuse information.
Base editing strategies to convert CAG to CAA diminish the disease-causing mutation in Huntington's disease
<p>An expanded CAG repeat in the huntingtin gene (<em>HTT</em>) causes Huntington's disease (HD). Since the length of uninterrupted CAG repeat, not polyglutamine, determines the age-at-onset in HD, base editing strategies to convert CAG to CAA are anticipated to delay onset by shortening the uninterrupted CAG repeat. Here, we developed base editing strategies to convert CAG in the repeat to CAA and determined their molecular outcomes and effects on relevant disease phenotypes. Base editing strategies employing combinations of cytosine base editors and gRNAs efficiently converted CAG to CAA at various sites in the CAG repeat without generating significant indels, off-target edits, or transcriptome alterations, demonstrating their feasibility and specificity. Candidate BE strategies converted CAG to CAA on both expanded and non-expanded CAG repeats without altering <em>HTT</em> mRNA and protein levels. In addition, somatic CAG repeat expansion, which is the major disease driver in HD, was significantly decreased in the liver by a candidate BE strategy treatment in HD knock-in mice carrying canonical CAG repeats. Notably, CAG repeat expansion was abolished entirely in HD knock-in mice carrying CAA-interrupted repeats, supporting the therapeutic potential of CAG-to-CAA conversion strategies in HD and potentially other repeat expansion disorders.</p>
CGG, CAG, and GAA: genome-wide comparison of the disease linked Trinucleotide short tandem repeat
Open the record for dataset details and reuse information.
Base editing strategies to convert CAG to CAA diminish the disease-causing mutation in Huntington's disease
Open the record for dataset details and reuse information.
RNA sequencing data from the prefrontal cortex and hippocampus of male (12 weeks old) hemizyguous CAG-HERV-W-env mice and wild-type controls
<p>RNA sequencing data from the prefrontal cortex (PFC) and hippocampus (HIPP) of male (12 weeks old) hemizyguous CAG<sup>HERV-Wenv </sup>mice ( C57BL6/J;129P2/Ola-Hprt mice; <em>n</em> = 3) relative to wild-type ( <em>n</em> = 3) littermates. Total RNA was extracted from prefrontal and hippocampal samples using the SPLIT RNA extraction kit (Lexogen, Austria) following the manufacturer’s recommendations and was sent to the Functional Genomics Center in Zurich (FGCZ) for quality control and RNA sequencing. The quality of the isolated RNA was determined with a Fragment Analyzer (Agilent, Santa Clara, California, USA). Only those samples with a 260 nm/280 nm ratio between 1.8–2.1, a 28S/18S ratio within 1.5–2, and RIN (>8) values qualified for a Poly-A enrichment strategy in order to generate the sequencing libraries applying the TruSeq mRNA Stranded Library Prep Kit (Illumina, Inc, California, USA). After Poly-A selection using Oligo-dT beads the mRNA was reverse-transcribed into cDNA. The cDNA was fragmented, end-repaired and poly-adenylated before ligation of TruSeq UD Indices (IDT, Coralville, Iowa, USA). The quality and quantity of the amplified sequencing libraries were validated using a Fragment Analyzer SS NGS Fragment Kit (1–6000 bp) (Agilent, Waldbronn, Germany). The equimolar pool of the samples was spiked into a NovaSeq6000 run targeting ~15M reads per sample on a S1 FlowCell (Novaseq S1 Reagent Kit, 100 cycles, Illumina, Inc, California, USA). Reads were quality-checked with FastQC. Sequencing adapters were removed with Trimmomatic and aligned to the reference genome and transcriptome of Mus Musculus (GENCODE, GRCm38,p5) with STAR v2.7.3. Distribution of the reads across genomic isoform expression was quantified using the R package GenomicRanges from Bioconductor Version 3.10. Minimum mapping quality, as well as minimum feature overlaps, was set to 10. Multi-overlaps were allowed. Differentially expressed genes (DEGs) were identified using the R package edgeR from Bioconductor Version 3.10, using a generalized linear model (glm) regression, a quasi-likelihood (QL) differential expression test and the trimmed means of M-values (TMM) normalization.</p>
FIGURE 6. Leucoagaricus aurantiovergens coll. CAG P.12-9 in Leucoagaricus cupresseoides (Agaricaceae), a new species in sect. Piloselli and L. aurantiovergens and L. pseudopilatianus redescribed from Italy
FIGURE 6. Leucoagaricus aurantiovergens coll. CAG P.12-9/8.39a. A–B. Basidiomes in the natural habitat; C. Basidiospores; D. Basidia; E. Cheilocystidia; F. Pileus covering elements. Scale bars: A–B = 2 cm, C–E = 10 μm, F = 50 μm. Photos by Alessia Tatti.
FIGURE 2. Leucoagaricus idae-fragum. A–F Fresh basidiomes. A Coll. MCVE 29362. B Coll. CAG P.12 in Molecular confirmation of Leucoagaricus idae-fragum (Agaricales, Agaricaceae), and notes on its morphological variability
FIGURE 2. Leucoagaricus idae-fragum. A–F Fresh basidiomes. A Coll. MCVE 29362. B Coll. CAG P.12_9/8.38. C–F Coll. MCVE 29342. E Pileus surface and annulus. F Green reaction of the pileus surface with ammonia (left) and NaOH (right). Scale bars: 10 mm. Photos: A by M. Filippa; B by A. Tatti; C–F by T. Lezzi.
Microalbuminuria Predicting CIAKI After CAG
ClinicalTrials.gov study NCT02808845. IPD Sharing: Not stated. Countries: 1. Publications: 12.
The CAG Bipolar the CAG Bipolar RCT
ClinicalTrials.gov study NCT04229875. IPD Sharing: NO. Countries: 1. Publications: 1.
Decitabine Combining Modified CAG Followed by HLA Haploidentical Peripheral Blood Mononuclear Cells Infusion for Elderly Patients With Acute Myeloid Leukemia(AML）
ClinicalTrials.gov study NCT01690507. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Screen of DM by OGTT in Subjects Receiving CAG or MDCT for CAD
ClinicalTrials.gov study NCT01198730. IPD Sharing: Not stated. Countries: 1. Publications: 4.
Data from: CAG repeat not polyglutamine length determines timing of Huntington's disease onset
Variable, glutamine-encoding, CAA interruptions indicate that a property of the uninterrupted HTT CAG repeat sequence, distinct from the length of huntingtin's polyglutamine segment, dictates the rate at which Huntington's disease (HD) develops. The timing of onset shows no significant association with HTT cis-eQTLs but is influenced, sometimes in a sex-specific manner, by polymorphic variation at multiple DNA maintenance genes, suggesting that the special onset-determining property of the uninterrupted CAG repeat is a propensity for length instability that leads to its somatic expansion. Additional naturally occurring genetic modifier loci, defined by GWAS, may influence HD pathogenesis through other mechanisms. These findings have profound implications for the pathogenesis of HD and other repeat diseases and question the fundamental premise that polyglutamine length determines the rate of pathogenesis in the "polyglutamine disorders."
Data from: The effect of CAG repeats length on differences in hirsutism among healthy Israeli women of different ethnicities
Purpose: Variations in the degree of hirsutism among women of different ethnic backgrounds may stem from multiple etiologies. Shorter length of the polymorphic CAG repeats of the androgen receptor (AR) gene may be associated with increased activity of the receptor leading to hirsutism. We hypothesized that there are ethnic differences in the degree of hirsutism that is unrelated to androgen levels among Israeli women, and that the CAG repeats length may contribute to these differences. Anti-androgenic therapies, such as spironolactone, could be suggested if a shorter CAG repeats length is found to affect the difference in the degree of hirsutism between the ethnic groups. Methods: Healthy Israeli Jewish women aged 18-45 years of Ashkenazi and non-Ashkenazi origin were invited to participate. Hirsutism was assessed using the simplified Ferriman-Gallwey (sFG) score, and serum total testosterone levels were measured as well. The CAG repeats length was determined by PCR. Methylation-sensitive methods were used to detect the fractional activity of each allele, and the weighted mean was calculated for the CAG repeats length. Results: One-hundred and eight women were recruited (49 Ashkenazi and 59 non-Ashkenazi). The Ashkenazi women had a significantly lower degree of hirsutism (P<0.01), lower mean BMI (P=0.003), total testosterone levels (P=0.017), and longer weighted bi-allelic CAG repeats mean (P=0.015) compared to non-Ashkenazi women. For the group as a whole, there was a significant negative correlation between the number of CAG repeats in the AR gene and the sFG score, while the number of repeats was not related to testosterone levels. Stepwise logistic regression revealed that ethnic origin and the CAG repeats length were the strongest factors affecting hirsutism (P<0.001, P=0.03, respectively). Conclusions: There is a significant difference in the degree of hirsutism between Ashkenazi and non-Ashkenazi women in Israel that is partially explained by CAG repeats length.
Supplementary material 1 from: Berneck BVM, Giaretta AA, Brandão RA, Cruz CAG, Haddad CFB (2017) The first species of Aplastodiscus endemic to the Brazilian Cerrado (Anura, Hylidae). ZooKeys 642: 115-130. https://doi.org/10.3897/zookeys.642.10401
Analyzed sound files; from Ariovaldo A. Giaretta collection :
Supplementary material 2 from: Berneck BVM, Giaretta AA, Brandão RA, Cruz CAG, Haddad CFB (2017) The first species of Aplastodiscus endemic to the Brazilian Cerrado (Anura, Hylidae). ZooKeys 642: 115-130. https://doi.org/10.3897/zookeys.642.10401
List of voucher specimens, GenBank accession numbers, and locality data :
Figure 7 from: Berneck BVM, Giaretta AA, Brandão RA, Cruz CAG, Haddad CFB (2017) The first species of Aplastodiscus endemic to the Brazilian Cerrado (Anura, Hylidae). ZooKeys 642: 115-130. https://doi.org/10.3897/zookeys.642.10401
Figure 7 - One of the four most parsimonious trees recovered. Asterisks indicate 100% Parsimony Jackknife absolute frequency; only values above 75% are shown. See Suppl. material 2 for details and complete locality names.
Figure 6 from: Berneck BVM, Giaretta AA, Brandão RA, Cruz CAG, Haddad CFB (2017) The first species of Aplastodiscus endemic to the Brazilian Cerrado (Anura, Hylidae). ZooKeys 642: 115-130. https://doi.org/10.3897/zookeys.642.10401
Figure 6 - A The habitat of Aplastodiscus lutzorum sp. n.: flooded gallery forests B A male in calling activity at Fazenda Água Limpa, Brasília, Distrito Federal, Brazil.
Figure 5 from: Berneck BVM, Giaretta AA, Brandão RA, Cruz CAG, Haddad CFB (2017) The first species of Aplastodiscus endemic to the Brazilian Cerrado (Anura, Hylidae). ZooKeys 642: 115-130. https://doi.org/10.3897/zookeys.642.10401
Figure 5 - Geographic distribution of Aplastodiscus cochranae (pink triangles), Aplastodiscus perviridis (green circle), and Aplastodiscus lutzorum sp. n. (blue squares, blue star indicates its type-locality). Note that Aplastodiscus lutzorum shows a disjunctive distribution regarding the other Aplastodiscus species, occurring deep within Cerrado Biome.
Figure 1 from: Berneck BVM, Giaretta AA, Brandão RA, Cruz CAG, Haddad CFB (2017) The first species of Aplastodiscus endemic to the Brazilian Cerrado (Anura, Hylidae). ZooKeys 642: 115-130. https://doi.org/10.3897/zookeys.642.10401
Figure 1 - Holotype of Aplastodiscus lutzorum sp. n. (AAG-UFU 864). A Lateral view of head B dorsal view of head C plantar view D palmar view. Scale bar 12 mm.
Figure 4 from: Berneck BVM, Giaretta AA, Brandão RA, Cruz CAG, Haddad CFB (2017) The first species of Aplastodiscus endemic to the Brazilian Cerrado (Anura, Hylidae). ZooKeys 642: 115-130. https://doi.org/10.3897/zookeys.642.10401
Figure 4 - A comparison between duration and frequency peak time of Aplastodiscus lutzorum (N = 12 males) and Aplastodiscus perviridis (N = 5 males). In both samples, calls are of topotypes.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.