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160 results for “CRISPR activation”
tRNA anticodon cleavage by target-activated CRISPR-Cas13a effector
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Data from: Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells
<p>Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In <em>Drosophila</em> cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes, approaches for performing genome-wide gain-of-function screens are still lacking. Here, we describe a pooled CRISPR activation (CRISPRa) screening platform in <em>Drosophila</em> cells and apply this method to both focused and genome-wide screens to identify rapamycin resistance genes. The screens identified three genes as novel rapamycin resistance genes: a member of SLC16 family of monocarboxylate transporters (<em>CG8468)</em>, a member of the lipocalin protein family (<em>CG5399</em>), and a zinc finger C2H2 transcription factor (<em>CG9932</em>). Mechanistically, we demonstrate that <em>CG5399</em> overexpression activates the RTK-Akt-mTOR signaling pathway and that activation of insulin receptor (InR) by <em>CG5399</em> requires cholesterol and clathrin-coated pits at the cell membrane. This study establishes a novel platform for functional genetic studies in <em>Drosophila</em> cells.</p>
Data from: Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells
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Phenotypically active ORF and CRISPR consensus profiles
This hosts the files on phenotypically active genes for JUMP, derived from https://zenodo.org/records/14025602 after filtering samples with no matches above a given threshold (see filenames for thresholds).
Phenotypically active ORF and CRISPR consensus profiles
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CRISPR activation of endogenous genes reprograms fibroblasts into cardiovascular progenitor cells
GEO Series GSE158084. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
A single-cell transcriptomics CRISPR-activation screen identifies new epigenetic regulators of the zygotic genome activation programm (10X Genomics CRISPRa screen dataset)
GEO Series GSE135621. Mus musculus. 27 samples. Type: Expression profiling by high throughput sequencing.
Effect of CRISPR activation of Zbtb7b, Vps72, Gba, or Mrpl9 in liver tumorigenesis in mice
GEO Series GSE250070. Mus musculus. 30 samples. Type: Expression profiling by high throughput sequencing.
CRISPR activation screens identify the SWI-SNF ATPases as suppressors of ferroptosis
GEO Series GSE245249. Homo sapiens. 65 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other.
Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations [RNA-Seq human]
GEO Series GSE118843. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
Activation of the imprinted Prader-Willi Syndrome locus by CRISPR-based epigenome editing [bisulphite-seq]
GEO Series GSE285300. Homo sapiens. 18 samples. Type: Methylation profiling by high throughput sequencing.
A CRISPR-Activation CROP-seq Screen Identifies HMGN1 as a Dosage Sensitive Regulator of Heart Defects in Down Syndrome (scATAC-Seq)
GEO Series GSE271445. Homo sapiens. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
CHANGE-seq reveals the genome-wide activity of CRISPR-Cas9 [RNA-seq]
GEO Series GSE149362. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
A viral guide RNA delivery system for CRISPR-based transcriptional activation and heritable targeted DNA demethylation
GEO Series GSE148934. Arabidopsis thaliana. 12 samples. Type: Methylation profiling by high throughput sequencing.
CRISPR activation and interference screens decode stimulation responses in primary human T cells [Bulk RNA-seq]
GEO Series GSE174284. Homo sapiens. 16 samples. Type: Expression profiling by high throughput sequencing.
CRISPR activation enables high fidelity reprogramming into human pluripotent stem cells (single cells)
GEO Series GSE162528. Homo sapiens. 11 samples. Type: Expression profiling by high throughput sequencing.
Single-cell gene expression profiling of MYCN-amplified neuroblastoma cells following CRISPR activation of ferroptosis regulators
GEO Series GSE210762. Homo sapiens. 4 samples. Type: Expression profiling by high throughput sequencing; Other.
Rational design of immune gene therapy combinations via in vivo CRISPR activation screening of tumor microenvironment modulators [CRISPR]
GEO Series GSE255515. Mus musculus. 16 samples. Type: Other.
A single-cell transcriptomics CRISPR-activation screen identifies new epigenetic regulators of the zygotic genome activation progrmme (bulk RNA-seq of arrayed CRISPRa validations and bulk RNA-seq of P
GEO Series GSE135512. Mus musculus. 57 samples. Type: Expression profiling by high throughput sequencing.
MNK3 cells with CRISPR interference or activation at the CLN3 gene
GEO Series GSE313942. Mus musculus. 24 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.