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84 results for “CRISPR knockout”
Data set: Genome scale CRISPR Cas9a knockout screen reveals genes that controls glioblastoma susceptibility to the alkylating agent temozolomide (TMZ).
<p>Glioblastoma is the deadliest of all primary brain tumor with a very poor survival outcome. Alkylating agent such as temozolomide is used as a mainstay drug used in treating of glioblastoma patients including radiation, tumor treating field and surgery. However, this drug offers little to no benefit for the glioblastoma patients. Hence, the objective of the generation of this data is to understand and unravel genes that controls glioblastoma susceptibility and resistance to temozolomide using an unbiased genome scale CRISPR Cas9a knockout screen</p>
CRISPR knockout of EZH1 in AML cell line
<p>We wanted to ensure the specificity of the EZH1 antibody that I was working with in previous posts, as well as creating a useful reagent to use in future experiments by generating a CRISPR knockout line of EZH1.</p>
Data from: CRISPR-Cas9 mediated CD133 knockout inhibits colon cancer invasion through reduced epithelial-mesenchymal transition
We previously reported that CD133, as a putative cancer stem cell marker, plays an important role in cell proliferation and invasion in colon cancer. To understand the role of CD133 expression in colon cancer, we evaluated the inhibitory effect of CD133 in colon cancer cells. In this study, we generated CD133knockout colon cancer cells (LoVo) using the CRISPR-Cas9 gene editing system. CD133+ colon cancer cells (LoVo) were infected with the lentiviral vector carrying CD133 gRNA and purified cell by culturing single cell colonies. CD133knockout cells was validated by western blot and flow cytometry analysis. In functional study, we observed a significant reduction in cell proliferation and colony formation in CRISPR-Cas9 mediated CD133 knockout cells in compare with control (P < 0.001). We also found the anticancer effect of stattic was dependent on CD133 expression in colon cancer cells. Although CD133knockout cells could not completely block the tumorigenic property, they showed remarkable inhibitory effects on the ability of cell migration and invasion (P < 0.001). In addition, we examined the epithelial mesenchymal transition (EMT)-related protein expression by western blot. The result clearly showed a loss of vimentin expression in CD133knockout cells. Therefore, CRISPR-Cas9 mediated CD133knockout can be an effective treatment modality for CD133+ colon cancer through reducing the characteristics of cancer stem cells.
Data from: CRISPR-Cas9 mediated CD133 knockout inhibits colon cancer invasion through reduced epithelial-mesenchymal transition
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CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
GEO Series GSE252605. Komagataella phaffii. 12 samples. Type: Expression profiling by high throughput sequencing.
Effects of PHGDH CRISPR knockout on gene expression in glioblastoma-associated endothelial cells
GEO Series GSE199376. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Genome-wide pooled CRISPR knockout screen for novel regulators of macrophage efferocytosis.
GEO Series GSE212008. Mus musculus. 15 samples. Type: Other.
RNA-SEQ assay for wild type and CRISPR induced endoglin knockout human pulmonary artery smooth muscle cells (PASMC)
GEO Series GSE78271. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Gene expression profiles of Ofd1 and Kif3a mutant SMB21 cells generated by CRISPR knockout
GEO Series GSE103525. Mus musculus. 7 samples. Type: Expression profiling by high throughput sequencing.
Human kinome CRISPR knockout screen in H358 parental and sotorasib resistant H358 cells
GEO Series GSE275314. Homo sapiens. 6 samples. Type: Other.
CRISPR library knockout screening identified candidate genes whose loss of function confers lapatinib resistance in HER2-amplified GC cells.
GEO Series GSE148668. Homo sapiens. 12 samples. Type: Other.
Integrating CRISPR knockout, interference, and activation to dissect the functional role and underlying signaling of microRNA-23a-27a-24-2 cluster in hepatocellular carcinoma
GEO Series GSE199332. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Genome-wide CRISPR Knockout Screen of Healthy Donor CART19-28ζ Cells in the Manuscript IL-4 Drives Exhaustion of CD8+ CART Cells
GEO Series GSE273299. Homo sapiens. 6 samples. Type: Other.
Systematic evaluation of C. elegans lincRNAs with CRISPR knockout mutants [ChIP-Seq]
GEO Series GSE122973. Caenorhabditis elegans. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Enhanced genome-wide knockout screens in bacteria with CRISPR base editors
GEO Series GSE225335. Escherichia coli. 27 samples. Type: Other.
CRISPR Knockout screens for the identification of X-linked genes that drive sex differences in mouse embryonic stem cells
GEO Series GSE142350. Mus musculus. 32 samples. Type: Other.
Forward genetic screening in engineered colorectal cancer organoids identifies novel regulators of metastasis [crispr gRNA knockout screening 3]
GEO Series GSE263582. Mus musculus. 15 samples. Type: Other.
An arrayed CRISPR screen identifies knockout combinations improving antibody productivity in HEK293 cells
GEO Series GSE288118. Homo sapiens. 28 samples. Type: Expression profiling by high throughput sequencing.
CRISPR genome editing of human dendritic cells (treatments of knockout dendritic cells)
GEO Series GSE161466. Homo sapiens. 47 samples. Type: Expression profiling by high throughput sequencing.
Chicken IRF7 knockout by CRISPR-Cas9 in DF-1 and in vitro H6N2 infection followed by RNA-seq
GEO Series GSE119590. Gallus gallus. 24 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.