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23
datasets available to search
ShareScore release 0.9.0
Dataset results
23 results for “Cell based assay”
Development of ferret immune repertoire reference resources and single-cell-based high- throughput profiling assays
<p>We performed long read transcriptome sequencing of ferret splenocyte and lymph node samples full-length, non-chimeric circular consensus sequencing (CCS) reads to obtain over 120,000 high-quality immunoglobin (Ig) and T cell receptor (TCR) transcripts.</p>
A targeted metabolomics-based assay using human induced pluripotent stem cell-derived cardiomyocytes identifies structural and functional cardiotoxicity potential
<p>Implementing screening assays that identify functional and structural cardiotoxicity earlier in the drug development pipeline has the potential to improve safety and the cost and time required to bring new drugs to market. In this study, a metabolic biomarker-based assay was developed that predicts the cardiotoxicity potential of a drug based on changes in the metabolism and viability of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM). Assay development and testing was conducted in two phases: (1) biomarker identification and (2) targeted assay development. In the first phase, metabolomic data from hiPSC-CM spent media following exposure to 66 drugs was used to identify biomarkers that identified both functional and structural cardiotoxicants. Four metabolites that represent different metabolic pathways (arachidonic acid, lactic acid, 2'-deoxycytidine, and thymidine) were identified as indicators of cardiotoxicity. In phase two, a targeted, exposure-based biomarker assay was developed that measured these metabolites and hiPSC-CM viability across an eight-point concentration curve. Metabolite-specific predictive thresholds for identifying the cardiotoxicity potential of a drug were established and optimized for balanced accuracy or sensitivity. When predictive thresholds were optimized for balanced accuracy, the assay predicted the cardiotoxicity potential of 81 drugs with 86% balanced accuracy, 83% sensitivity, and 90% specificity. Alternatively, optimizing the thresholds for sensitivity yields a balanced accuracy of 85%, 90% sensitivity, and 79% specificity. This new hiPSC-CM-based assay provides a paradigm that can identify structural and functional cardiotoxic drugs that could be used in conjunction with other endpoints to provide a more comprehensive evaluation of a drug's cardiotoxicity potential.</p>
Developing and Study of on-line Biotoxicity Sensing and Control Unit for Wastewater Treatment, based on a Cell Viability Monitoring Assay
<p>The quality of industrial, municipal or hospital waters varies greatly throughout the days and seasons. Because usually one of the first steps of water treatment plants (WWTPs) is biological treatment (after mechanical cleaning) it is vital that this step operates at full capacity. However, toxic compounds such as antibiotics, which are in use for human consumption or animal agriculture can greatly disrupt this stage of cleaning due to their antibiological nature of operation.</p> <p>Technion has developed a system in which toxicity to bacteria is measured on-line. It is based on following spectral changes in a dye, which is reduced as part of bacterial metabolism, which depends on the level of toxicity. The measured toxicity values are fed to a PID controller, which controls the operation of an AOP reactor.</p> <p>This system was developed as part of "Project O", a H2020 program of the European Commission.</p>
A targeted metabolomics-based assay using human induced pluripotent stem cell-derived cardiomyocytes identifies structural and functional cardiotoxicity potential
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Data from: Screening of cytotoxic or cytostatic flavonoids with quantitative FUCCI-based cell cycle assay
The Fluorescence-Ubiquitin Cell Cycle Indicator (FUCCI) system can be used not only to study gene expression at a specific cell cycle stage, but also to monitor cell cycle transitions in real time. In this study, we used a single clone of FUCCI-expressing HeLa cells (FUCCI-HeLa cells) and monitored the cell cycle in individual live cells over time by determining the ratios between red fluorescence (RF) of RFP-Cdt1 and green fluorescence (GF) of GFP-Geminin. Cytotoxic and cytostatic compounds, the latter of which induced G2 or mitotic arrest, were identified based on periodic cycling of the RF/GF and GF/RF ratios in FUCCI-HeLa cells treated with anticancer drugs. With this cell cycle monitoring system, ten flavonoids were screened. Of these, apigenin and luteolin, which have a flavone backbone, were cytotoxic, whereas kaempferol, which has a flavonol backbone, was cytostatic and induced G2 arrest. In summary, we developed a system to quantitatively monitor the cell cycle in real time. This system can be used to identify novel compounds that modulate the cell cycle and to investigate structure–activity relationships.
Study for Detection of Donor-derived Cell-free DNA After Renal Transplantation Using Devysers NGS-based Chimerism Assay.
ClinicalTrials.gov study NCT05226936. IPD Sharing: YES. Countries: 0. Publications: 3.
Data from: Screening of cytotoxic or cytostatic flavonoids with quantitative FUCCI-based cell cycle assay
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Inference and effects of barcode multiplets in droplet-based single-cell assays
GEO Series GSE143197. Homo sapiens. 1 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Development of a novel cell-based assay to diagnose recurrent Focal and Segmental Glomerulosclerosis
GEO Series GSE117669. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells
GEO Series GSE216926. Homo sapiens. 14 samples. Type: Other.
Phenotypic and Functional Characteristics of Human Schwann Cells as Revealed by Cell-Based Assays and RNA-SEQ
GEO Series GSE133716. Homo sapiens; Rattus norvegicus. 6 samples. Type: Expression profiling by high throughput sequencing.
Grouping of UVCB Substances with Dose-Response Transcriptomics Data from Human Cell-Based Assays
GEO Series GSE186121. Homo sapiens. 5934 samples. Type: Expression profiling by high throughput sequencing.
Optimization of Human Neural Progenitor Cells for an Imaging-Based High-Throughput Phenotypic Profiling Assay for Developmental Neurotoxicity Screening
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Yeast cell-based transport assay for the functional characterization of human 4F2hc-LAT1 and ‐LAT2, and LAT1 and LAT2 substrates and inhibitors
<p>Data to Kantipudi & Fotiadis (2021), <em>Front. Mol. Biosci. </em>(2021) <strong>8</strong>, 676854.</p>
Evaluation of Two Cell-based Assays for Diagnosing MOG-IgG Associated Disorders
ClinicalTrials.gov study NCT06617962. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Initial Evaluation of a Telomerase-based Circulating Tumor Cell Assay in Bladder Cancer Cohorts
ClinicalTrials.gov study NCT02246738. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Cell-based Assays for Antibody-mediated Transplant Rejection
ClinicalTrials.gov study NCT03916822. IPD Sharing: NO. Countries: 1. Publications: 0.
Development of a potency test assay for CD34+ cell-based for acute myocardial infarction
GEO Series GSE220865. Homo sapiens. 15 samples. Type: Expression profiling by high throughput sequencing.
Sequencing-based Functional Assays for Classification of BRCA2 Variants in Mouse ES Cells
GEO Series GSE244578. Mus musculus. 176 samples. Type: Genome variation profiling by high throughput sequencing.
Human embryonic stem cell based neuro-developmental toxicity assay: response to valproic acid and carbamazepine exposure
GEO Series GSE64123. Homo sapiens. 93 samples. Type: Expression profiling by array.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.