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208 results for “Cell polarity”
Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 4 relative to Figure 7 – ArhGEF11 CRISPR interference
<p><span>Raw image files (TIFF format), corresponding 2D-cartographies (_2Dmap.tiff files) and metadata files for 2D-cartographies (.xml files, readable with the opensource software Icy), relative to <strong>Figure 7B </strong>and<strong> Figure 7 - Figure Supplement 6</strong> (see <strong>Materials and Methods — Morphological and morphometric analysis of aortic and hemogenic cells</strong>).</span></p> <p><span>The source data comprises for each 48 - 55 hpf <em>Tg(kdrl:eGFP-JAM3b; kdrl:nls-mKate2)</em> zebrafish embryo 3 z-stack and 2D cartographies (segments 1 to 3) encompassing the whole length of the aorta, for control condition (n = 2 individuals) and morpholino splicing interference condition (n = 2 individuals). For z-stacks of both control and morphant conditions, two fluorescence channels were acquired, corresponding to the nuclear mKate2 expressed in endothelial cells and the eGFP-JAMs signal localized at the intercellular junctions of endothelial cells. Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.3 µm. 2D-cartographies were obtained using the Icy plugin “TubeSkinner”, and the semi-manual segmentation of all aortic cells can be uploaded from the corresponding metadata file on the 2D-cartographies using the load ROI function of Icy.</span></p>
Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 3 relative to Figure 7 – ArhGEF11 morpholino splicing interference
<p><span>Raw image files (TIFF format), corresponding 2D-cartographies (_2Dmap.tiff files) and metadata files for 2D-cartographies (.xml files, readable with the opensource software Icy), relative to <strong>Figure 7A </strong>and<strong> Figure 7 - Figure Supplement 5</strong> (see <strong>Materials and Methods — Morphological and morphometric analysis of aortic and hemogenic cells</strong>).</span></p> <p><span>The source data comprises for each 48 - 55 hpf <em>Tg(kdrl:eGFP-JAM2a; kdrl:nls-mKate2)</em> zebrafish embryo 3 z-stack and 2D cartographies (segments 1 to 3) encompassing the whole length of the aorta, for control condition (n = 2 individuals) and morpholino splicing interference condition (n = 3 individuals). For z-stacks of both control and morphant conditions, two fluorescence channels were acquired, corresponding to the nuclear mKate2 expressed in endothelial cells and the eGFP-JAMs signal localized at the intercellular junctions of endothelial cells. Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.3 µm. 2D-cartographies were obtained using the Icy plugin “TubeSkinner”, and the semi-manual segmentation of all aortic cells can be uploaded from the corresponding metadata file on the 2D-cartographies using the load ROI function of Icy.</span></p>
Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 1 relative to Figure 3
<p><span>Raw image files (TIFF format) relative <strong>to Figure 3</strong> (see <strong>Materials and Methods — Dt-runx1 phenotype analysis – cell count</strong>).</span></p> <p><span>The source data comprises for each 52 - 55 hpf zebrafish embryo 3 z-stack (segments 1 to 3) encompassing the whole length of the aorta, for control condition (<em>Tg(Kdrl:Gal4;UAS:RFP), </em>n = 3 individuals) and mutant condition (<em>Tg(kdrl:Gal4;UAS:RFP;4xNR:dt-runx1-eGFP), </em>n = 7 individuals). For control condition, one fluorescence channel was acquired, corresponding to the cytoplasmic RFP expressed in endothelial cells. For mutant condition, two fluorescence channels were acquired, corresponding first to the cytoplasmic RFP expressed in endothelial cells using the same reporter as for the control condition, and second the cleaved cytoplasmic GFP reporting the expression of our dt-runx1 mutant construct in endothelial cells. Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.3 µm.</span></p>
Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 2 relative to Figure 4
<p><span>Raw image files (TIFF format) and segmented 3D images (.ims, Imaris proprietary files) relative to <strong>Figure 4</strong> and <strong>Figure 4 Figure Supplement 3</strong> (see <strong>Materials and Methods — RNAscope image analysis – Pard3</strong>).</span></p> <p><span>The source data comprises for each 52 - 55 hpf zebrafish embryos 2 z-stack (segments 1 to 2) encompassing the whole length of the aorta, for control condition (<em>Tg(Kdrl:eGFP), </em>n = 7 individuals) and mutant condition (<em>Tg(kdrl:Gal4; 4xNR:dt-runx1-eGFP), </em>n = 12 individuals). For both control and mutant conditions, two fluorescence channels are displayed, corresponding to the cytoplasmic GFP expressed in endothelial cells (in green) and the RNAscope signal (OPAL-570, in magenta). Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.4 µm. The .ims files contain the 3D rendering of the z-stacks as well as the segmentations of Pard3ba mRNA RNAscope spots (in magenta), in the aorta (Spots 1 Selection) or outside (Spots 1), as well as the segmentation of endothelial cells (green) (Cells 1) and hemogenic endothelial cells (Cells 1 Cell export).</span></p>
Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis
<p>Complete dataset for the manuscript "Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis" including images for illustrations and quantifications. </p>
Data from: Amoeboid cells undergo durotaxis with soft end polarized NMIIA
Open the record for dataset details and reuse information.
Scupa: Single-cell unified polarization assessment of immune cells using the single-cell foundation model
<p>This dataset contains the processed scRNA-seq data and code for single-cell unified polarization assessment. Please refer to the article "Scupa: Single-cell unified polarization assessment of immune cells using the single-cell foundation model" for the detailed data and method description.</p> <p>*.rds: the processed scRNA-seq datasets with Universal Cell Embeddings saved as a Seurat object. The Universal Cell Embeddings are saved in the assay 'uce'.</p> <ul> <li>immune_dict_uce.rds: Immune Dictionary.</li> <li>ifnb_treatment_uce.rds: human PBMCs treated with IFN-beta.</li> <li>macrophage_stimulation_uce.rds: human macrophages treated with one or two of IFN-beta, IFN-gamma, TNF-alpha and IL-4.</li> <li>il2_treatment_uce.rds: mouse spleen CD8+ T cells treated with IL-2 or anti-PD-L1.</li> <li>pan_cancer_myeloid_uce.rds: tumor-infiltrating myeloid cells across seven cancer types.</li> </ul> <p>notebooks.zip: Jupyter notebooks containing code for training models and applications to multiple datasets.</p>
Vimentin supports cell polarization by enhancing centrosome function and microtubule acetylation
<p>Cell polarity is important for controlling cell shape, motility, and cell division processes. Vimentin intermediate filaments are important for cell migration and cell polarization in mesenchymal cells and assembly of vimentin and microtubule networks is dynamically coordinated, but the precise details of how vimentin mediates cell polarity remain unclear. Here, we characterize the effects of vimentin on the structure and function of the centrosome and the stability of microtubule filaments in wild-type and vimentin-null mouse embryonic fibroblasts (mEFs). We find that vimentin mediates the structure of the pericentrosomal material, promotes centrosome-mediated microtubule regrowth, and increases the level of stable acetylated microtubules in the cell. Loss of vimentin also impairs centrosome repositioning during cell polarization and migration processes that occur during wound closure. Our results suggest that vimentin modulates centrosome structure and function as well as microtubule network stability, which has important implications for how cells establish proper cell polarization and persistent migration.</p>
April 7, 2024 (v1) Image Open Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 5 relative to Figure 7 - Figure Supplement 4
<p>Source data file relative to <strong><span>Figure 7 – figure supplement 4 Panel A</span></strong></p> <p><span>Raw image of agarose gel showing the 2 alternative mRNAs encoding for ArhGEF11 in control animals (left track, control) and after injection of the MO at the one cell stage (right track, +MO at 2 and 5ng). The source data includes the raw files (native format .scn and open source format .tiff) as well as a pdf file showing both the full scale image and the cropped image selected for the figure.<br></span></p>
Evolution of plasma properties in 2D particle-in-cell simulations of pulsar polar caps as function of dipole inclination angle
<p>The video shows the evolution of plasma properties in polar cap region of neutron stars from initial simulation conditions to the quasi-periodic pair creation. Three inclination angles of magnetic dipole axis to the star rotation axis are investigate \iota = 0°, 45°, and 90°.</p> <p>The presented quantities are (in rows): Parallel current density, parallel electric field, parallel and perpendicular Poynting flux, electron and positron plasma density, and electron and positron plasma bulk momenta.</p> <p> </p>
Rawdata for publication: An Efficient and Stable Polarizing Agent for In-Cell Magic-Angle Spinning Dynamic Nuclear Polarization NMR Spectroscopy
<p>This is the raw dataset for the publication: An Efficient and Stable Polarizing Agent for In-Cell Magic-Angle Spinning Dynamic Nuclear Polarization NMR Spectroscopy. It contains the EPR and NMR data used in this publication. DOI of this pubcliation: 10.1021/acs.jpclett.4c02709</p>
Polatuzumab Vedotin, Rituximab, Ifosfamide, Carboplatin, and Etoposide (PolaR-ICE) as Initial Salvage Therapy for the Treatment of Relapsed/Refractory Diffuse Large B-Cell Lymphoma
ClinicalTrials.gov study NCT04665765. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Polarization of yeast cells under different pheromone induction regimens
Open the record for dataset details and reuse information.
Epithelial polarization by the core planar cell polarity complex is exclusively non-autonomous
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Vimentin supports cell polarization by enhancing centrosome function and microtubule acetylation
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Dataset related to article "Senescent thyrocytes and thyroid tumor cells induce M2-like macrophage polarization of human monocytes via a PGE2-dependent mechanism."
<p>BACKGROUND:</p> <p>Thyroid carcinoma includes several variants characterized by different biological and clinical features: from indolent microcarcinoma to undifferentiated and aggressive anaplastic carcinoma. Inflammation plays a critical role in thyroid tumors. Conditions predisposing to cancer, as well as oncogene activity, contribute to the construction of an inflammatory microenvironment that facilitates thyroid tumor progression. Moreover, oncogene-induced senescence, a mechanism tightly connected with inflammation, and able to restrain or promote cancer progression, is involved in thyroid cancer. The interactions between thyroid tumor cells and the microenvironment are not completely clarified.</p> <p>METHODS:</p> <p>We characterize in vitro the interplay between macrophages and senescent thyrocytes and tumor-derived cell lines, modeling early and late thyroid tumor stages, respectively. Purified peripheral blood-derived human monocytes were exposed to thyroid cell-derived conditioned medium (CM) and assessed for phenotype by flow cytometry. The factors secreted by thyroid cells and macrophages were identified by gene expression analysis and ELISA. The protumoral effect of macrophages was assessed by wound healing assay on K1 thyroid tumor cells. The expression of PTGS2 and M2 markers in thyroid tumors was investigated in publicly available datasets.</p> <p>RESULTS:</p> <p>Human monocytes exposed to CM from senescent thyrocytes and thyroid tumor cell lines undergo M2-like polarization, showing high CD206 and low MHC II markers, and upregulation of CCL17 secretion. The obtained M2-like macrophages displayed tumor-promoting activity. Among genes overexpressed in polarizing cells, we identified the prostaglandin-endoperoxide synthase enzyme (PTGS2/COX-2), which is involved in the production of prostaglandin E2 (PGE2). By using COX-2 inhibitors we demonstrated that the M2-like polarization ability of thyroid cells is related to the production of PGE2. Co-expression of PTGS2 and M2 markers is observed a significant fraction of human thyroid tumors.</p> <p>CONCLUSIONS:</p> <p>Our results demonstrate that both senescent thyrocytes and thyroid tumor cell lines trigger M2-like macrophage polarization that is related to PGE2 secretion. This suggests that the interaction with the microenvironment occurs at both early and late thyroid tumor stages, and favors tumor progression. The co-expression of PTGS2 gene and M2 markers in human thyroid carcinoma highlights the possibility to counteract tumor growth through COX-2 inhibition.</p>
Rapid Effects Linagliptin on Monocyte Polarization and Endothelial Progenitor Cells in Type 2 Diabetes
ClinicalTrials.gov study NCT01617824. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Telomere Length in Human Polar Body and Telomere Length in Cumulus Cells: A Clinical Validation Study
ClinicalTrials.gov study NCT04577560. IPD Sharing: YES. Countries: 1. Publications: 11.
Data from: The core planar cell polarity gene, Vangl2, directs adult corneal epithelial cell alignment and migration
This study shows that the core planar cell polarity (PCP) genes direct the aligned cell migration in the adult corneal epithelium, a stratified squamous epithelium on the outer surface of the vertebrate eye. Expression of multiple core PCP genes was demonstrated in the adult corneal epithelium. PCP components were manipulated genetically and pharmacologically in human and mouse corneal epithelial cells in vivo and in vitro. Knockdown of VANGL2 reduced the directional component of migration of human corneal epithelial (HCE) cells without affecting speed. It was shown that signalling through PCP mediators, dishevelled, dishevelled-associated activator of morphogenesis and Rho-associated protein kinase directs the alignment of HCE cells by affecting cytoskeletal reorganization. Cells in which VANGL2 was disrupted tended to misalign on grooved surfaces and migrate across, rather than parallel to the grooves. Adult corneal epithelial cells in which Vangl2 had been conditionally deleted showed a reduced rate of wound-healing migration. Conditional deletion of Vangl2 in the mouse corneal epithelium ablated the normal highly stereotyped patterns of centripetal cell migration in vivo from the periphery (limbus) to the centre of the cornea. Corneal opacity owing to chronic wounding is a major cause of degenerative blindness across the world, and this study shows that Vangl2 activity is required for directional corneal epithelial migration.
IN-CELL QUANTIFICATION OF DRUGS BY MAGIC ANGLE SPINNING DYNAMIC NUCLEAR POLARIZATION NMR
<p>Raw data</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.