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datasets available to search
ShareScore release 0.9.0
Dataset results
14 results for “Cellular nanoparticles”
Cellular uptake of silica and gold nanoparticles induces early activation of nuclear receptor NR4A1
<p>The approval of new nanomedicines requires a deeper understanding of the interaction between cells and nanoparticles (NPs). Silica (SiO<sub>2</sub>) and gold (Au) NPs have shown great potential in biomedical applications, such as the delivery of therapeutic agents, diagnostics, and biosensors. NP-cell interaction and internalization can trigger several cellular responses, including gene expression regulation. The identification of differentially expressed genes in response to NP uptake contributes to a better understanding of the cellular processes involved, including potential side effects. We investigated gene regulation in human macrophages and lung epithelial cells after acute exposure to spherical 60 nm SiO<sub>2</sub> NPs. SiO<sub>2</sub> NPs uptake did not considerably affect gene expression in epithelial cells, whereas five genes were up-regulated in macrophages. These genes are principally related to inflammation, chemotaxis, and cell adhesion. NR4A1, an important modulator of inflammation in macrophages, was found to be up-regulated. The expression of this gene was increased upon 1 h of macrophage exposure to spherical 50 nm AuNPs and 200 nm spherical SiO<sub>2</sub> NPs. NR4A1 can thus be an important immediate regulator of inflammation provoked by NP uptake in macrophages.</p>
Core-shell structured chitosan-polyethylenimine nanoparticles for gene delivery: Improved stability, cellular uptake, and transfection efficiency
<p>Gene therapy has emerged as a promising treatment option for various acquired and inherited diseases. The delivery of nucleic acids relies on so-called vectors that condense and encapsulate their cargo, generating stable nano-sized particles. Especially non-viral gene delivery systems are of increasing interest. However, accomplishing therapeutic levels of transgene expression and limited tolerability of these systems remain a challenge. Therefore, we investigate in the present study the improvement of nucleic acid delivery using depolymerized chitosan – polyethylenimine DNA core complexes (dCS-PEI/DNA). These core complexes are further entrapped into a variety of dCS-based shells, functionalized with poly(ethylene glycol) (PEG) spacers conjugated to ionic moieties (amino or carboxylate groups) and cell penetrating peptides. This modular approach allowed to evaluate the effect of the shell functional components on the physico-chemical particle characteristics and biological effects <em>in vitro</em>. The optimized ternary complex combines a core-dCS-LPEI/DNA complex with a shell consisting of dCS-PEG-COOH, which resulted in improved encapsulation of nucleic acid, accelerated cellular uptake, enhanced transfection efficiency, and superior transfection potency in human hepatoma HuH-7 cells and mouse primary hepatocytes. Effects on transgene expression are confirmed <em>in vivo</em> in wild-type mice following retrograde intrabiliary infusion. After administration to mice of only 100 ng complexed nanovector DNA, ternary complexes induce a high reporter gene signal for three days. We conclude that ternary core-shell structured particles comprising functionalized chitosan are a promising gene delivery technology for both <em>in vitro</em> as well as <em>in vivo </em>applications. The modular design will facilitate the development of chemically modified derivatives.</p>
Interactive effects of ZnO nanoparticles and temperature on molecular and cellular stress responses of the blue mussel Mytilus edulis
<p>We investigate the molecular and cellular stress responses of tissues (gills and digestive glands) in the mussels <em>Mytilus edulis </em>exposed for 21 days to either ambient (10 °C in winter and 15 °C in summer) or elevated (by 5 °C) temperature combined with different concentrations (0, 10, 100 µg l<sup>-1</sup>) of ZnO nanoparticles or dissolved zinc. In this data set, we used a biomarker-based approach focusing on oxidative stress indicators ( lipid peroxidation and protein carbonyls) and transcription pattern of marker genes in the pathways related to cell survival and proliferation (caspase 3, Bcl-2, p53, p38 and JNK) and inflammation (COX, NF-κB, and TGF-β) of <em>M. edulis</em>. </p>
How Can Giant Plasma Membrane Vesicles Serve as a Cellular Model for Controlled Transfer of Nanoparticles?
<p>Data underlying the figures in the publication “How Can Giant Plasma Membrane Vesicles Serve as a Cellular Model for Controlled Transfer of Nanoparticles?”, published in <em>Biomacromolecules</em>, <strong>2021</strong>, 22, 1, 106–115.</p> <p><a href="https://pubs.acs.org/doi/10.1021/acs.biomac.0c00624">https://pubs.acs.org/doi/10.1021/acs.biomac.0c00624</a></p> <p>Table of contents:</p> <p><strong>1. Figure 1</strong>; Zip file containing the data for <em>Figure 1</em>.</p> <p><strong>2. Figure 2</strong>; Zip file containing the TEM micrographs for <em>Figure 2</em>.</p> <p><strong>3. Figure 4</strong>; Zip file containing numerical data of the flow cytometry measurements and analysis for <em>Figure 4</em>.</p> <p><strong>4. Figure 5</strong>; Zip file containing the data for <em>Figure 5</em>.</p> <p><strong>5. Figure 6,7</strong>; Zip file containing numerical data for <em>Figures 6 </em>and <em>7</em>.</p>
Nanoparticle Cellular Internalization is Not Required for RNA Delivery to Mature Plant Leaves
<p>These datasets are used to produce the figures/graphs published in our article.</p>
Single-Cell Transcriptional Analysis of Murine Mesenteric Lymph Nodes Following Oral Lyso-phosphatidylserine Nanoparticle Administration Reveals Cellular Heterogeneity in Tolerance Features
GEO Series GSE302907. Mus musculus. 3 samples. Type: Expression profiling by high throughput sequencing.
Cellular landscapes of osteogenically differentiated valvular interstitial cells stimulated with XCT790-loaded nanoparticles
GEO Series GSE252267. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Lipid nanoparticles as adjuvant of Norovirus VLP vaccine augment cellular and humoral immune responses in a TLR 9- and type I IFN-dependent pathway
GEO Series GSE279372. Mus musculus. 28 samples. Type: Expression profiling by high throughput sequencing.
Single cell multiomic readouts reveal that cellular heterogeneity impacts lipid nanoparticle-mediated mRNA delivery
GEO Series GSE186395. Mus musculus. 1 samples. Type: Expression profiling by high throughput sequencing; Other.
Transcriptomic profiling reveals differential cellular response of copper oxide nanoparticles and polystyrene nanoplastics in perfused human placenta
GEO Series GSE220756. Homo sapiens. 16 samples. Type: Expression profiling by array.
Cellular response to protein-conjugated nanoparticles
GEO Series GSE23118. Homo sapiens. 16 samples. Type: Expression profiling by array.
Species-dependent in vivo mRNA delivery and cellular response to nanoparticles
GEO Series GSE178313. Mus musculus; Homo sapiens. 19 samples. Type: Expression profiling by high throughput sequencing.
Waterborne exposure of adult zebrafish to silver nanoparticles and ionic silver results in silver accumulation and effects at cellular and molecular levels
GEO Series GSE89653. Danio rerio. 27 samples. Type: Expression profiling by array.
Comparative Iron Oxide Nanoparticle Cellular Dosimetry and Response in Mice by the Inhalation and Liquid Cell Culture Exposure Routes
GEO Series GSE51417. Mus musculus. 60 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.