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73 results for “Cellular types”
Combined unsupervised and semi-automated supervised analysis of flow cytometry data reveals cellular fingerprint associated with newly diagnosed pediatric type 1 diabetes
<p>Type 1 diabetes is a chronic autoimmune disease resulting in an immune-mediated loss of pancreatic β-cells; however, an unbiased and reproducible profiling of type 1 diabetes-specific circulating immunome at disease onset has yet to be explored. In this study, fresh whole blood was collected from a pediatric cohort of 107 patients with new-onset type 1 diabetes, 85 relatives of patients with type 1 diabetes with 0-1 islet autoantibodies, 58 patients with celiac disease or autoimmune thyroiditis and 76 healthy controls. Up to 6 mL of blood was collected from each subject into a VACUETTE® TUBE 6 ml ACD-B (Greiner). Fresh whole blood underwent red blood cell lysis, was washed and stained with specific monoclonal antibodies. Fresh whole blood samples were stained with five panels of antibodies labelled as T cells, T&NK cells, B cells, Tregs and DCs/monos encompassing main subsets of T cells, NK cells, B cells, Tregs, DCs and monocytes detected using 26 surface markers and the intracellular marker forkhead box P3 (FoxP3); for the Treg panel, intracellular staining was performed after fixation and permeabilization. Cells were acquired on a BD FACSCanto-II flow cytometer equipped with FACSDiva software (Becton Dickinson, Franklin Lakes, NJ). </p>
Electrogenetic cellular insulin release for real-time glycemic control in type 1 diabetic mice
<p>Data underlying the figures in the publication “Electrogenetic cellular insulin release for real-time glycemic control in type 1 diabetic mice”, published in <em>Science</em>, <strong>2020</strong>, 368, 993-1001. <a href="https://science.sciencemag.org/content/368/6494/993">https://science.sciencemag.org/content/368/6494/993</a></p> <p>Table of contents:</p> <p><strong>1. Electrogenetics source data file</strong>; File openable with Graphpad Prism containing the source data for the main <em>Figures: 1-4 </em>(Transgene expression by SEAP measurement), <em>5b, 6a, 6f</em> (Insulin by ELISA), <em>5c, 6b, 6e </em>(NanoLuc luciferase), 7c (NanoLuc luciferase in vivo) and <em>7a, 7d</em> (Glycemia).</p> <p><strong>Transgene expression by SEAP measurement</strong></p> <p>Data for main <em>Figures 1, 2, 3, 4</em>.</p> <p>SEAP (human placental secreted alkaline phosphatase) levels were profiled in cell culture supernatants using a colorimetric assay. 100 µL 2x SEAP assay buffer (20 mM homoarginine, 1 mM MgCl2, 21% diethanolamine, pH 9.8) was mixed with 80 µL heat-inactivated (30 min at 65°C) cell culture supernatant. After the addition of 20 µL substrate solution (120 mM p-nitrophenyl phosphate; cat. no. AC128860100, Thermo Fisher Scientific), the absorbance time course was recorded for 45 min at 405 nm and 37°C using a Tecan Genios PRO plate reader (cat. no. P97084; Tecan Group AG, Maennedorf, Switzerland) and the SEAP levels were determined as follows: first, absorbance change over time (slope) was calculated. According to the Beer–Lambert’s law, absorbance is proportional to the concentration of a colored compound and depends on the light path length (d) and extinction coefficient (ε) (ε for p-nitrophenyl (εpNP) = 18.600 M−1 cm−1). Enzymatic activity EA [U/L] was calculated from the equation: EA = slope × dilution factor × εpNP−1 × d−1 </p> <p>Values in the file present determined SEAP levels.</p> <p><strong>Insulin by ELISA</strong></p> <p>Data for <em>Figures 5b, 6a, 6f</em>.</p> <p>Values in the file present Insulin level as determined by ELISA kit. The assay was performed according to manufacturer’s instructions.</p> <p><strong>NanoLuc luciferase </strong></p> <p>Data for <em>Figures 5c, 6b, 6e</em>.</p> <p>NanoLuc® luciferase was quantified in cell culture supernatants using the Nano-Glo® Luciferase Assay System (cat. no. N1110; Promega, Duebendorf, Switzerland). In brief, 7.5 µL of cell culture supernatant was added per well of a black 384-well plate and mixed with 7.5 µL substrate-containing assay buffer. Total luminescence was quantified using a Tecan Genios PRO plate reader (Tecan Group AG).</p> <p>Values in the file present measured luminescence levels.</p> <p><strong>NanoLuc luciferase in vivo </strong></p> <p>Data for <em>Figure 7c.</em></p> <p>Aliquots of 15 µL of whole-blood samples were diluted in 5 μL of 50 mM EDTA and frozen at -20 °C until NanoLuc® quantification as described above.</p> <p>Values in the file present measured luminescence levels normalized to time point 0 (Normalization individually for each mouse).</p> <p><strong>Glycemia </strong></p> <p>Data for <em>Figures 7a, 7d</em>.</p> <p>Blood glucose level was determined using a glucometer (Contour®Next, Bayer Healthcare, Leverkusen, Germany).</p> <p>Values in the file present measured glucose levels.</p> <p><strong>2. Figure 7b</strong>; Data for <em>Figure 7b</em>. File openable with Graphpad Prism.</p> <p>Blood glucose level was determined using a glucometer (Contour®Next, Bayer Healthcare, Leverkusen, Germany).</p> <p>Values in the file present measured glucose levels.</p> <p><strong>3. Figure 6c and 6d</strong>; Excel file with the data for <em>Figures 6c, 6d</em>.</p> <p>NanoLuc® luciferase was quantified in cell culture supernatants using the Nano-Glo® Luciferase Assay System (cat. no. N1110; Promega, Duebendorf, Switzerland). In brief, 7.5 µL of cell culture supernatant was added per well of a black 384-well plate and mixed with 7.5 µL substrate-containing assay buffer. Total luminescence was quantified using a Tecan Genios PRO plate reader (Tecan Group AG).</p> <p>Values in the file present measured luminescence levels.</p>
Effect of Sodium-glucose Cotransporter-2 Inhibitor in Cellular Senescence in Patients With Cardiovascular Diseases or Type 2 Diabetes
ClinicalTrials.gov study NCT05975528. IPD Sharing: NO. Countries: 1. Publications: 16.
Changes in cellular microRNA expression induced by porcine circovirus type 2-encoded proteins.
GEO Series GSE60206. Sus scrofa. 4 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Circulating cell-free, methylated DNA reveals tissue-specific, cellular damage from radiation treatment [Mouse Reference Cell-type MCC-seq]
GEO Series GSE200185. Mus musculus. 8 samples. Type: Methylation profiling by high throughput sequencing.
Single-cell transcriptomic resolution of human pancreatic islets reveals cellular states and intercellular interactions associated with type 1 diabetes
GEO Series GSE148073. Homo sapiens. 14 samples. Type: Expression profiling by high throughput sequencing.
Circulating cell-free, methylated DNA reveals tissue-specific, cellular damage from radiation treatment [Human Reference Cell-type MCC-seq]
GEO Series GSE200093. Homo sapiens. 12 samples. Type: Methylation profiling by high throughput sequencing.
Circulating, cell-free methylated DNA reveals cellular sources of allograft injury after liver transplant (LTR reference cell type)
GEO Series GSE262274. Homo sapiens. 14 samples. Type: Methylation profiling by high throughput sequencing.
Annelid adult cell type diversity and their pluripotent cellular origins
GEO Series GSE230505. Pristina leidyi. 4 samples. Type: Expression profiling by high throughput sequencing.
The effect of cellular context on miR-155 mediated gene regulation in four major immune cell types (RNA-Seq)
GEO Series GSE116348. Mus musculus. 24 samples. Type: Expression profiling by high throughput sequencing.
Type I CRISPR-Cas provides robust immunity but incomplete attenuation of phage-induced cellular stress
GEO Series GSE186673. Prodigiosinella confusarubida. 36 samples. Type: Expression profiling by high throughput sequencing.
Circulating cell-free, methylated DNA reveals tissue-specific, cellular damage from radiation treatment [Human Reference Cell-type RNA-seq]
GEO Series GSE200095. Homo sapiens. 5 samples. Type: Expression profiling by high throughput sequencing.
Induced pluripotent stem cell derived neuronallineages as human cellular models for Herpes Simplex Virus, type 1 (HSV-1) infections
GEO Series GSE46042. Homo sapiens. 6 samples. Type: Expression profiling by array.
Mitochondrial dynamics define muscle fiber type by modulating cellular metabolism pathways
GEO Series GSE228362. Mus musculus. 3 samples. Type: Expression profiling by high throughput sequencing.
Cell-type-specific expression of tRNAs in the brain regulates cellular homeostasis
GEO Series GSE239759. Mus musculus. 22 samples. Type: Other.
Single-cell RNA sequencing of peripheral blood mononuclear cells reveals complex cellular signalling signatures of type 2 diabetes mellitus
GEO Series GSE255566. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
The effect of cellular context on miR-155 mediated gene regulation in four major immune cell types
GEO Series GSE116561. Mus musculus. 95 samples. Type: Expression profiling by high throughput sequencing.
Profiling cellular diversity in sponges informs animal cell type and nervous system evolution
GEO Series GSE134912. Spongilla lacustris. 5 samples. Type: Expression profiling by high throughput sequencing.
Cellular and viral transcription profiles in Equine Papillomavirus Type 2 positive squamous cell carcinomas
GEO Series GSE123533. Equus caballus. 8 samples. Type: Expression profiling by high throughput sequencing.
Single cell approaches define the murine leptomeninges:cortical brain interface as a distinct cellular neighborhood comprised of neural and nonneural cell types
GEO Series GSE296134. Mus musculus. 8 samples. Type: Other.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.