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3 results for “Chick chorioallantoic membrane”
Results from: Angiogenic property of silk fibroin scaffolds with adipose-derived stem cells on chick chorioallantoic membrane
<p>Angiogenesis is a key step in tissue regeneration and repair. Biomaterials that allow or promote angiogenesis are thus beneficial. In this study, angiogenic properties of salt-leached silk fibroin (SF) scaffolds seeded with human adipose stem cells (hADSCs) were studied using chick chorioallantoic membrane (CAM) as a model. The hADSC-seeded SF scaffolds (SF-hADSC) with porosity of 77.34 ± 6.96 % and pore diameter of 513.95 ± 4.99 µm were implanted on CAM of chick embryos that were on embryonic day 8 (E8) of development. The SF-hADSC scaffolds induced a spoke-wheel pattern of capillary network indicative of angiogenesis, which was evident since E11. Moreover, ingrowth of blood vessels into the scaffolds was seen in histological sections. The unseeded scaffolds induced the same extent of angiogenesis later on E14. In contrast, the control group could not induce angiogenesis to the same extent even as late. <i>In vitro</i> cytotoxicity tests and <i>in vivo</i> angioirritative study reaffirmed the biocompatibility of the scaffolds. This work highlighted that the biocompatible SF-hADSC scaffolds accelerates angiogenesis, and hence they can be a promising biomaterial for regeneration of tissues that require angiogenesis.</p>
Results from: Angiogenic property of silk fibroin scaffolds with adipose-derived stem cells on chick chorioallantoic membrane
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U87 cells implanted on a chick CAM (Chorioallantoic Membrane)
GEO Series GSE43674. Gallus gallus; Homo sapiens. 97 samples. Type: Expression profiling by array.
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