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22 results for “Clostridium thermocellum”
Increasing the thermodynamic driving force of the phosphofructokinase reaction in Clostridium thermocellum
<p>Glycolysis is an ancient, widespread, and highly conserved metabolic pathway that converts glucose into pyruvate. In the canonical pathway, the phosphofructokinase (PFK) reaction plays an important role in controlling flux through the pathway. <em>Clostridium thermocellum</em> has an atypical glycolysis and uses pyrophosphate (PPi) instead of ATP as the phosphate donor for the PFK reaction. The reduced thermodynamic driving force of the PPi-PFK reaction shifts the entire pathway closer to thermodynamic equilibrium, which has been predicted to limit product titers. In this work, we replace the PPi-PFK reaction with an ATP-PFK reaction. We demonstrate that the local changes are consistent with thermodynamic predictions: the ratio of fructose 1,6-bisphosphate to fructose-6-phosphate increases, and the reverse flux through the reaction (determined by <sup>13</sup>C labeling) decreases. The final titer and distribution of fermentation products, however, do not change, demonstrating that the thermodynamic constraints of the PPi-PFK reaction are not the sole factor limiting product titer.</p>
Maximizing long-term biohydrogen production with Clostridium thermocellum in high solids lignocellulosic biomass fermentations
Open the record for dataset details and reuse information.
Characterization and amelioration of filtration difficulties encountered in metabolomic studies of Clostridium thermocellum at elevated sugar concentrations
<p>Datasets and python codes to generate figures for the research paper published in Applied and Environmental Microbiology (DOI:10.1128/aem.00406-23).</p>
Comparative transcriptomics of Clostridium thermocellum wild type (Δhpt) and Type I glutamine synthetase (Clo1313_2031) deletion strains using RNA-seq
GEO Series GSE78219. Acetivibrio thermocellus DSM 1313. 8 samples. Type: Expression profiling by high throughput sequencing.
Clostridium thermocellum Transcriptomic Profiles after Exposure to Furfural or Heat Stress
GEO Series GSE40402. Acetivibrio thermocellus ATCC 27405. 43 samples. Type: Expression profiling by array.
Understanding the Regulatory Networks of Three LacI Transcriptional Repressors in Clostridium thermocellum DSM1313
GEO Series GSE68423. Acetivibrio thermocellus DSM 1313. 36 samples. Type: Expression profiling by high throughput sequencing.
Metabolic inhibition of Clostridium thermocellum M1570 by pentose sugars.
GEO Series GSE74918. Acetivibrio thermocellus. 8 samples. Type: Expression profiling by high throughput sequencing.
Clostridium thermocellum DSM 1313 Transcriptional Responses to Redox Perturbation
GEO Series GSE71465. Acetivibrio thermocellus ATCC 27405; Acetivibrio thermocellus DSM 1313. 32 samples. Type: Expression profiling by array.
Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation
GEO Series GSE29554. Acetivibrio thermocellus ATCC 27405. 39 samples. Type: Expression profiling by array.
Contribution of cell-bound and cell-free cellulosomal versus non-cellulosomal systems in deconstruction of cellulosic biomass by Clostridium thermocellum
GEO Series GSE63883. Acetivibrio thermocellus ATCC 27405; Acetivibrio thermocellus DSM 1313. 14 samples. Type: Expression profiling by array.
Significant gene expression differences between sessile biofilms and planktonic cells of Clostridium thermocellum
GEO Series GSE87407. Acetivibrio thermocellus ATCC 27405. 8 samples. Type: Expression profiling by high throughput sequencing.
Clostridium thermocellum ATCC27405 Transcriptomic Profiles during growth on Populus and switchgrass
GEO Series GSE47010. Acetivibrio thermocellus ATCC 27405. 8 samples. Type: Expression profiling by array.
The molecular response of Clostridium thermocellum ATCC 27405 to ethanol shock
GEO Series GSE25236. Acetivibrio thermocellus ATCC 27405. 30 samples. Type: Expression profiling by array.
Global gene expression patterns in Clostridium thermocellum from microarray analysis of chemostat culture on cellulose or cellobiose
GEO Series GSE22426. Acetivibrio thermocellus ATCC 27405. 11 samples. Type: Expression profiling by array.
Transcriptomic profile of Clostridium thermocellum ATCC27405 using switchgrass as carbon source
GEO Series GSE26926. Acetivibrio thermocellus ATCC 27405. 17 samples. Type: Expression profiling by array; Expression profiling by genome tiling array.
Sulfate assimilation in Clostridium thermocellum
GEO Series GSE100047. Acetivibrio thermocellus DSM 1313. 12 samples. Type: Expression profiling by high throughput sequencing.
Transcriptomics comparison and transcriptional architecture improvement of Clostridium thermocellum ATCC27405 using systems biology data and bioinformatics approaches
GEO Series GSE26889. Acetivibrio thermocellus ATCC 27405. 12 samples. Type: Expression profiling by genome tiling array.
Improved growth of Clostridium thermocellum AG553 via medium supplementation with formate and strain evolution
GEO Series GSE78287. Acetivibrio thermocellus DSM 1313. 24 samples. Type: Expression profiling by high throughput sequencing.
Transcriptomic adaptations of Clostridium thermocellum strains evolved to grow on xylose
GEO Series GSE276070. Acetivibrio thermocellus DSM 1313. 15 samples. Type: Expression profiling by high throughput sequencing.
Comparison of gene expression profiles in three Clostridium thermocellum strains: DSM 1237, 2360 and 4150
GEO Series GSE51745. Acetivibrio thermocellus. 29 samples. Type: Expression profiling by array; Expression profiling by high throughput sequencing.
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