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67 results for “Cordycipitaceae”
Fig. 3 in Effects of a pathogenic Beauveria bassiana (Hypocreales: Cordycipitaceae) strain on detoxifying and protective enzyme activities in Xylotrechus rusticus (Coleoptera: Cerambycidae) larvae
Fig. 3. Effects of infection by Beauveria bassiana strain BbCC01 on protective enzyme activity in Xylotrechus rusticus larvae over time. A. Catalase (CAT). B. Peroxidase (POD). C. Superoxide dismutase (SOD). Data are expressed as mean ± SE (n = 3). Different letters indicate significant differences between means (P <0.05).
Fig. 4 in Effects of a pathogenic Beauveria bassiana (Hypocreales: Cordycipitaceae) strain on detoxifying and protective enzyme activities in Xylotrechus rusticus (Coleoptera: Cerambycidae) larvae
Fig. 4. Change of the protein content in Xylotrechus rusticus larvae infected with Beauveria bassiana strain BbCC01. Data are expressed as mean ± SE (n = 3). Different letters indicate significant differences between means (P <0.05).
Fig. 2 in Effects of a pathogenic Beauveria bassiana (Hypocreales: Cordycipitaceae) strain on detoxifying and protective enzyme activities in Xylotrechus rusticus (Coleoptera: Cerambycidae) larvae
Fig. 2. Effects of infection by Beauveria bassiana strain BbCC01 on detoxifying enzyme activity in Xylotrechus rusticus larvae over time. A. Carboxylesterase (CarE). B. Glutathione S-transferase (GST). C. Acetylesterase (AchE). Data are expressed as mean ± SE (n = 3). Different letters indicate significant differences between means (P <0.05).
Fig. 2 in Lethal and sub-lethal effects of Beauveria bassiana (Cordycipitaceae) strain NI8 on Chrysoperla rufilabris (Neuroptera: Chrysopidae)
Fig. 2. Cumulative mortality of Chrysoperla rufilabris females at 3, 5, and 10 d exposed to Beauveria bassiana strain NI8 at different concentrations (spores per mm2) under laboratory conditions. Insects were fed with a Lygus species solid diet afer being sprayed with fungus. Columns within the group labeled with a different letter were significant different at P = 0.05 (Tukey Honest Significant Difference test).
FIGURE 2. Gibellula flava GNJ20200814-46 in Gibellula flava sp. nov. (Cordycipitaceae, Hypocreales), a new pathogen of spider from China
FIGURE 2. Gibellula flava GNJ20200814-46 (asexual morph): a–c. Fungus on spider. k, l. Conidiophores and conidial head. j. Conidial head. m. Conidia. WFS20190625-25 (sexual morph): d. Stroma with ascomata. e. Perithecium ostioles (arrow). f. Perithecium. g. Ascus with thickened apical cap (arrow). h. Ascus and ascospores. i. Part-ascospores. Scale bars: f = 50 μm, j, k, l = 10 μm, g, h, i, m = 5 μm.
FIGURE 1 in Gibellula flava sp. nov. (Cordycipitaceae, Hypocreales), a new pathogen of spider from China
FIGURE 1. The maximum likelihood (ML) tree derived from multigene sequences of Gibellula spp. and allied taxa retrieved from GenBank with Hypocrea lutea (ATCC 208838) and H. voglmayrii (CBS117710) as outgroup. Numbers at the significant nodes represent ML bootstrap values and Bayesian posterior probabilities greater than 80%. Bold lines in the tree represent 100% of two values.
FIGURE 2. Lecanicillium subprimulinum a in Lecanicillium subprimulinum (Cordycipitaceae, Hypocreales), a novel species from Baoshan, Yunnan
FIGURE 2. Lecanicillium subprimulinum a White mycelium associated with the ophioceras-like taxon on a submerged wood. b Culture plate (above, left/reverse, right). c Conidiophores on culture. d–e Verticillate conidiophores and conidiogenous cells (arrowed node). f–g Conidiophore with conidia. h–j Conidia. Scale bars: a = 20 mm, b–c = 20 μm, d–g = 10 μm, i–j = 5 μm.
FIGURE 1 in Lecanicillium subprimulinum (Cordycipitaceae, Hypocreales), a novel species from Baoshan, Yunnan
FIGURE 1. Maximum likelihood phylogenetic tree generated from analysis of a combined LSU, SSU, tef1-α and ITS sequences dataset for 38 taxa of Lecanicillium. Simplicillium lanosoniveum is the outgroup taxon. ML support values greater than 70% (BSML, left), Bayesian posterior probabilities greater than 0.95 (BYPP, middle) and MP bootstrap value higher than 70% (BSMP, right) are indicated near the nodes. The strain numbers are noted after the species names. Ex-type strains are indicated in bold. Isolates from this study are indicated in red.
FIGURE 1 in Lecanicillium coprophilum (Cordycipitaceae, Hypocreales), a new species of fungus from the feces of Marmota monax in China
FIGURE 1. Phylogenetic tree derived from Bayesian analysis based on a combined dataset sequence of ITS+SSU+TEF+ LSU+RPB1+RPB2, depicting the relationships of the new species Lecanicillium coprophilum with closely related taxa. The significant Bayesian posterior probability (≥95%) and Bootstrap percentages over 50% are shown on the respective branches. Simplicillium lanosoniveum CBS 704.86 was used as the outgroup taxon.
FIGURE 2 in Lecanicillium coprophilum (Cordycipitaceae, Hypocreales), a new species of fungus from the feces of Marmota monax in China
FIGURE 2. Lecanicillium coprophilum (CGMCC 3.18986). a. Strain grown on different media after 8 days of incubation at 30 °C; b–d. Phialides; e–f. Swollen hyphae; g–h. Conidia; i. Dictyochlamydospores. Bars: b–i = 10 μm.
FIGURE 2 in Lecanicillium gracile (Cordycipitaceae), a new species isolated from mineral building materials
FIGURE 2. Lecanicillium gracile (CBS 142821, ex-type). a–c 14 days-old (25 °C, dark regime) colonies on PDA, PCA and MYA in 9 cm Petri plates, respectively. d–f Reverses of 14 days-old (25 °C, dark regime) colonies on PDA, PCA and MYA in 9 cm Petri plates, respectively. g–h Phialides and conidia (SEM). i–j Conidia. Scale bars: g–j = 10 μm.
FIGURE 1 in Lecanicillium gracile (Cordycipitaceae), a new species isolated from mineral building materials
FIGURE 1. Two-gene-based phylogeny (ITS rDNA+TEF1a) of Lecanicillium and closely related species. Our new isolates are marked in bold. Bayesian topology is displayed with the PP/ML support values over each node. Thickened branches indicated strong combined support (PP> 0.94, ML> 90). T – type/ex-type strains.
FIGURE 4 in Lecanicillium gracile (Cordycipitaceae), a new species isolated from mineral building materials
FIGURE 4. Temperature-dependent growth profiles of Lecanicillium gracile isolates. Colony diam. after 14 d on PDA (n=3, mean±SD).
FIGURE 3 in Lecanicillium gracile (Cordycipitaceae), a new species isolated from mineral building materials
FIGURE 3. Lecanicillium gracile (CBS 142821, ex-type). a–c Prostrate conidiophores with solitary phialides or phialides produced in whorls (LM). d Short erect conidiophore with phialides (LM). e–f Prostrate conidiophores with phialides produced in whorls (LM). g–h Phialides secondarily born on the preceeding phialides (LM). i Conidia (LM). j A drawing of prostrate conidiophores with phialides. k A drawing of conidiophores with phialides and conidia. Scale bars: a = 50 μm; b–k = 10 μm.
FIGURE 4 in Gibellula aurea sp. nov. (Ascomycota, Cordycipitaceae): a new golden spiderdevouring fungus from a Brazilian Atlantic Rainforest
FIGURE 4. Genetic distance within the genus Gibellula. Heatmaps were generated with sequences from each genomic region obtained from GenBank and the specimens from this study. Genetic distances between sequences are displayed from the lowest (red) to the highest (blue) distance. (a) Genetic distance within the small nuclear ribosomal subunit (SSU); (b) within the large nuclear ribosomal subunit (LSU); (c) translation elongation factor 1-α (TEF); and (d) RNA polymerase II second largest subunit (RPB2). Closer sequences are connected by the dendrogram on top and left in each heatmap.
FIGURE 3 in Gibellula aurea sp. nov. (Ascomycota, Cordycipitaceae): a new golden spiderdevouring fungus from a Brazilian Atlantic Rainforest
FIGURE 3. Placement of Gibellula aurea within the genus. Phylogenetic trees were based on concatenated sequences of SSU, LSU, TEF, and RPB2 markers. This tree topology resulted from a Maximum Likelihood, although Bayesian Inference displayed the same topology for most of the clades (Fig. S1). Numbers on branches indicate bootstrap values (ML>50%) followed by posterior probabilities (BI>0.70). Nucleotide substitutions per site are indicated by the scale bar. The phylogenetic tree includes 33 SSU, 63 LSU, 77 TEF, and 37 RPB2 sequences. Sequences from GenBank are exhibited by the name followed by their voucher numbers. For G. aurea specimens sequenced in this study, we show the name in bold (code: LBMCF). Formerly described species that split into single clades were also highlighted. The species Hevansia novoguineensis (NHJ 11923) was used as an outgroup.
FIGURE 2 in Gibellula aurea sp. nov. (Ascomycota, Cordycipitaceae): a new golden spiderdevouring fungus from a Brazilian Atlantic Rainforest
FIGURE 2. Diversity of host spiders parasitized by Gibellula aurea. (a) and (b) Corinnidae; but differ by abdomen shape, body size, and shape of the front legs; (c) Macrophyes pacoti (Anyphaenidae); (d) Anyphaenidae. Scale bars: a, b, c, d = 1 mm.
FIGURE 1 in Gibellula aurea sp. nov. (Ascomycota, Cordycipitaceae): a new golden spiderdevouring fungus from a Brazilian Atlantic Rainforest
FIGURE 1. Morphological description of Gibellula aurea. (a) Spider Macrophyes pacoti (Anyphaenidae) parasitized by G. aurea exhibiting one synnema (arrow) growing from the host's abdomen. (b–g) G. aurea visualized in the light microscope and (h–l) in the fluorescent microscope with UV light. (b, h) Inflated and fertile tip of synnema; (c) Conidiophore, stipe with verrucose wall, conidial head spherical; (d) Conidial head globose, vesicle ellipsoidal, metulae ellipsoidal, and phialides cylindrical; (e, j) Conidia ellipsoidal, with apiculate ends; (f, i) Granulomanus-like conidiophores; (g) Conidiogenous cells; (k) Spider leg (brown) covered by hyphae (blue) at the beginning of the infection; (l) Setae of spider leg involved by fungal hyphae. Scale bars: b, k, h = 100 µm, l = 50 µm, c, d, e, f, g, i, j = 10 µm.
FIGURE 5 in Gibellula aurea sp. nov. (Ascomycota, Cordycipitaceae): a new golden spiderdevouring fungus from a Brazilian Atlantic Rainforest
FIGURE 5. Intra- and interspecific genetic distances among sequences. (a) Proportion of intra- and interspecific of each genomic region (SSU, LSU, TEF and RPP2); (b) interspecific genetic distances among the sequences identified at the species level within the closest clade to Gibellula aurea.
FIGURE 3 in Akanthomyces zaquensis (Cordycipitaceae, Hypocreales), a new species isolated from both the stroma and the sclerotium of Ophiocordyceps sinensis in Qinghai, China
FIGURE 3. Akanthomyces zaquensis (CGMCC 19934) on PDA, similar to those on MEA and PCA. a–d. Phialides and conidia; e–f. conidia. Scale bars: a–f = 10 μm.
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