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zenodo40/100

Contrasting phylogeographic patterns of mitochondrial and genome-wide variation in the groundwater amphipod (Crangonyx islandicus) that survived the Ice age in Iceland

<p>In this zip &quot;DataAvailability.zip&quot; all the data and parameter files, as well as R scripts and bash codes used to perform the analyses of the mitochondrial and the RADseq data are provided. A ReadMe file is available at the root describing the content of the different folders and files.</p> <p>Below is the abstract of the associated manuscript:</p> <p>Analysis of phylogeographic patterns have often been based on mitochondrial DNA variation, but recent analyses dealing with nuclear DNA have in some instances revealed mito-nuclear discordances and complex evolutionary histories. These enigmatic scenarios which may involve stochastic lineage sorting, ancestral hybridization, past dispersal, and secondary contacts are increasingly scrutinized with a new generation of genomic tools such as RADseq, which pose also additional analytical challenges. Here, we revisited the previously inconclusive phylogeographic history, showing mito-nuclear discordance, of an endemic groundwater amphipod from Iceland, <em>Crangonyx islandicus,</em> which is the only metazoan known to have survived the Pleistocene beneath the glaciers. Previous studies based on three DNA markers documented a mitochondrial scenario with the main divergence occurring between populations in northern Iceland and an ITS scenario with the main divergence between the south and north. We used double digest restriction-site-associated DNA sequencing (ddRADseq) to clarify this mito-nuclear discordance by applying several statistical methods while estimating the sensitivity to different analytical approaches (data-type, differentiation indices and base call uncertainty). A majority of nuclear markers and methods support the ITS divergence. Nevertheless, a more complex scenario emerges, possibly involving introgression led by male-biased dispersal among northern locations or mitochondrial capture which may have been further strengthened by natural selection.</p>

opencc-by-4.0Jan 2023View details →
zenodo32/100

FIGURE 5 in Crangonyx islandicus sp. nov., a subterranean freshwater amphipod (Crustacea, Amphipoda, Crangonyctidae) from springs in lava fields in Iceland

FIGURE 5. Crangonyx islandicus sp. nov., female paratype, 6.2 mm, Vatnsvik. A, pereopod 6. B, coxa of pereopod 6. C, pereopod 7. D, spines distally on carpus of pereopod 7. E, pleopod 1. F, pleopod 2. G, pleopod 3. Scale: A–C = 0.5 mm; D = 0.1 mm; E–G = 0.5 mm.

opennotspecifiedDec 2006View details →
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FIGURE 2 in Crangonyx islandicus sp. nov., a subterranean freshwater amphipod (Crustacea, Amphipoda, Crangonyctidae) from springs in lava fields in Iceland

FIGURE 2. Crangonyx islandicus sp. nov., female paratype, 6.2 mm, Vatnsvik. A, antenna 1. B, distal part of flagellum of antenna 1. C, accessory flagellum of antenna 1. D, antenna 2. E, upper lip. F, left mandible, G, lacina mobilis and incisor. H, mandibular palp. I, lower lip. J, maxilla 1. K, maxilla 2. Scale: A, B = 0.5; C–K = 0.1 mm.

opennotspecifiedDec 2006View details →
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FIGURE 7. Sites with C in Crangonyx islandicus sp. nov., a subterranean freshwater amphipod (Crustacea, Amphipoda, Crangonyctidae) from springs in lava fields in Iceland

FIGURE 7. Sites with C. islandicus sp. nov. in Iceland. The large recent glaciers, i.e. Langjökull, Hofsjökull and Vatnajökull, are shown, as well as the large lakes, i.e. Lake Thingvallavatn and Lake Myvatn. Recent lava fields (since last glaciation; &lt;18.000 years, shaded area) are shown as well as the plate boundaries. Source: Institute of Earth Sciences, University of Iceland.

opennotspecifiedDec 2006View details →
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FIGURE 6 in Crangonyx islandicus sp. nov., a subterranean freshwater amphipod (Crustacea, Amphipoda, Crangonyctidae) from springs in lava fields in Iceland

FIGURE 6. Crangonyx islandicus sp. nov., female paratype, 6.2 mm, Vatnsvik. A, uropod 1. B, uropod 2. C, uropod 3. D, uropod 3 and telson. E, telson. Scale: A–D = 0.5 mm; E = 0.1 mm.

opennotspecifiedDec 2006View details →
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FIGURE 3 in Crangonyx islandicus sp. nov., a subterranean freshwater amphipod (Crustacea, Amphipoda, Crangonyctidae) from springs in lava fields in Iceland

FIGURE 3. Crangonyx islandicus sp. nov., female paratype, 6.2 mm, Vatnsvik. A, maxilliped. B, outer plate of maxilliped. C, inner plate of maxilliped. D, gnathopod 1. E, propodus and dactylus of gnathopod 1. F, short spines medially on gnathopod 1, in relation to largest two lateral bifid spines. G, gnathopod 2. H, distal part of propodus and dactylus of gnathopod 2. Scale: A = 0.5 mm; B, C, E, H = 0.5 mm; D, G = 0.1 mm.

opennotspecifiedDec 2006View details →
zenodo32/100

Fig. 8 Crangonyx parhobbsi n in A new species rises from beneath Florida: molecular phylogenetic analyses reveal cryptic diversity among the metapopulation of Crangonyx hobbsi Shoemaker, 1941 (Amphipoda: Crangonyctidae)

Fig. 8 Crangonyx parhobbsi n. sp.; holotype female, Madison Blue Spring, Madison County, Florida (UFID 051869), 7.25 mm: A, pereopod 4. Crangonyx parhobbsi n. sp.; paratype female, Madison Blue Spring, Madison County, Florida (UFID 051869), 7.45 mm: B, pereopod 5; C, pereopod 6; D, pereopod 7. Scale bars represent 1 mm

opennotspecifiedJun 2020View details →
zenodo32/100

Fig. 1 in A new species rises from beneath Florida: molecular phylogenetic analyses reveal cryptic diversity among the metapopulation of Crangonyx hobbsi Shoemaker, 1941 (Amphipoda: Crangonyctidae)

Fig. 1 Multilocus Bayesian phylogeny of selected members of the Crangonyctoidea. Posterior probability is indicated by colored diamonds (black 0.90–1.0, gray 0.89–0.80, white 0.79–0.70). Inset (upper) Crangonyx hobbsi, female, Devil's Eye Spring, Gilchrist County, Florida (YPM IZ 105321), 8.82 mm, scale bar represents

opennotspecifiedJun 2020View details →
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Fig. 6 Crangonyx parhobbsi n in A new species rises from beneath Florida: molecular phylogenetic analyses reveal cryptic diversity among the metapopulation of Crangonyx hobbsi Shoemaker, 1941 (Amphipoda: Crangonyctidae)

Fig. 6 Crangonyx parhobbsi n. sp.; holotype female, Madison Blue Spring, Madison County, Florida (UFID 051869), 7.25 mm: A, upper lip; B, lower lip; C, maxilla 1 (outer plate spine-teeth enlarged); D, maxilla 2 (outer plate apical serrate seta enlarged); E, maxilliped (inner plate apical margin enlarged). Scale bars represent 0.5 mm

opennotspecifiedJun 2020View details →
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Fig. 10 Crangonyx parhobbsi n in A new species rises from beneath Florida: molecular phylogenetic analyses reveal cryptic diversity among the metapopulation of Crangonyx hobbsi Shoemaker, 1941 (Amphipoda: Crangonyctidae)

Fig. 10 Crangonyx parhobbsi n. sp.; allotype male, Madison Blue Spring, Madison County, Florida (UFID 051870), 5.87 mm: A, antenna 1 accessory flagellum; B, antenna 2 (single calceolus enlarged); C, gnathopod 1 (palmar margin and dactylus enlarged); D, gnathopod 2 (palmar margin and dactylus enlarged). Scale bars 0.5 mm (A), 1 mm (B–D)

opennotspecifiedJun 2020View details →
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FIGURE 4. Crangonyx ephemerus n in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 4. Crangonyx ephemerus n. sp.; Holotype female, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52586), 6.21 mm: A, upper lip; B, lower lip; C, maxilla 1 (outer plate spine-teeth enlarged); D, maxilla 2; E, maxilliped (inner plate apical margin enlarged). Scale bars represent 0.5 mm.

opennotspecifiedOct 2019View details →
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FIGURE 17 in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 17. Principal component analysis (PCA) scatterplot based on 16 morphometric variables collected from Crangonyx ephemerus n. sp., C. pseudoephemerus n. sp., and populations of C. floridanus. Axes PC 1 (uropod 1 peduncle outer robust setae number) and PC 2 (body length) explain 59.2% and 16.8% of variation, respectively.

opennotspecifiedOct 2019View details →
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FIGURE 13. Crangonyx pseudoephemerus n in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 13. Crangonyx pseudoephemerus n. sp.; Holotype female, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52591), 6.24 mm: A, pereopod 4; B, pereopod 5; C, pereopod 6; D, pereopod 7; E, pleopod 1 (coupling hooks enlarged); F, pleopod 2 coupling hooks; G, pleopod 3 coupling hooks. Scale bars represent 1 mm.

opennotspecifiedOct 2019View details →
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FIGURE 2 in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 2. Habitus images of Crangonyx spp. examined. A, Crangonyx ephemerus n. sp.; Holotype female, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52586), 6.21 mm; Crangonyx ephemerus n. sp.; B, Allotype male, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52587), 3.85 mm; C, Crangonyx pseudoephemerus n. sp.; Holotype female, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52591), 6.24 mm; D, Crangonyx pseudoephemerus n. sp.; Allotype male, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52594), 4.16 mm. Scale bar represents 1 mm.

opennotspecifiedOct 2019View details →
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FIGURE 1 in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 1. Distribution of Crangonyx ephemerus n. sp. and C. pseudoephemerus n. sp. A, Location of type locality, St. Marks Headwaters Greenway, in Leon County, Florida. B, Photograph of type locality, March 2018.

opennotspecifiedOct 2019View details →
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FIGURE 3. Crangonyx ephemerus n in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 3. Crangonyx ephemerus n. sp.; Holotype female, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52586), 6.21 mm: A, antenna 1 (aesthetasc enlarged); B, antenna 2; C, left mandible (palp omitted); D, right mandible (lacinia mobilis enlarged). Scale bars: 1 mm (A, B), 0.5 mm (C, D).

opennotspecifiedOct 2019View details →
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FIGURE 9. Crangonyx ephemerus n in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 9. Crangonyx ephemerus n. sp.; Allotype male, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52587), 3.85 mm: A, pereopod 5 basis posterior margin; B, pereopod 6 basis posterior margin; C, pereopod 7 basis; D, uropod 1; E, uropod 2 (outer ramus comb-spines enlarged); F, uropod 3 (inner ramus enlarged); G, telson. Scale bars: 1 mm (A-E), 0.5 mm (F-G).

opennotspecifiedOct 2019View details →
zenodo32/100

FIGURE 16. Crangonyx pseudoephemerus n in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 16. Crangonyx pseudoephemerus n. sp.; Allotype male, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52594), 4.16 mm: A, pereopod 5 basis posterior margin; B, pereopod 6 basis posterior margin; C, pereopod 7 basis; D, uropod 1; E, uropod 2 (outer ramus comb-spines enlarged); F, uropod 3 (inner ramus enlarged); G, telson. Scale bars: 1 mm (A–E), 0.5 mm (F–G).

opennotspecifiedOct 2019View details →
zenodo32/100

FIGURE 8. Crangonyx ephemerus n in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 8. Crangonyx ephemerus n. sp.; Allotype male, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52587), 3.85 mm: A, antenna 2 (single calceolus enlarged); B, gnathopod 1 (palmar margin and dactylus enlarged); C, gnathopod 2 (palmar margin and dactylus enlarged). Scale bars represent 1 mm.

opennotspecifiedOct 2019View details →
zenodo32/100

FIGURE 6. Crangonyx ephemerus n in Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the "Crangonyx floridanus complex "

FIGURE 6. Crangonyx ephemerus n. sp.; Holotype female, St. Marks Headwaters Greenway, Leon County, Florida (UFID 52586), 6.21 mm: A, pereopod 4; B, pereopod 5; C, pereopod 6; D, pereopod 7; E, pleopod 1 (coupling hooks enlarged); F, pleopod 2 coupling hooks; G, pleopod 3 coupling hooks. Scale bars represent 1 mm.

opennotspecifiedOct 2019View details →

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