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11 results for “Crithidia”
Genetic modification of the bee parasite Crithidia bombi for improved visualization and protein localization
<p><em>Crithidia bombi </em>is a trypanosomatid parasite that infects several species of bumble bees (<em>Bombus</em> spp.), by adhering to their intestinal tract. <em>Crithidia bombi </em>infection impairs learning and reduces survival of workers and overwintering queens. Although there is extensive research on the ecology of this host-pathogen system, we understand far less about the mechanisms that mediate internal infection dynamics. <em>Crithidia bombi</em> infects hosts by attaching to the hindgut via the flagellum, and one previous study found that a nectar secondary compound removed the flagellum, preventing attachment. However, approaches that allow more detailed observation of parasite attachment and growth would allow us to better understand factors mediating this host-pathogen relationship. We established techniques for genetic manipulation and visualization of cultured <em>C. bombi.</em> Using constructs established for <em>Crithidia fasciculata, </em>we successfully generated <em>C. bombi </em>cells expressing ectopic fluorescent transgenes using two different selectable markers. To our knowledge, this is the first genetic modification of this species. We also introduced constructs that label the mitochondrion and nucleus of the parasite, showing that subcellular targeting signals can function across parasite species to highlight specific organelles. Finally, we visualized fluorescently tagged parasites <em>in vitro</em> in both their swimming and attached forms, and <em>in vivo </em>in bumble bee (<em>Bombus impatiens</em>) hosts. Expanding our cell and molecular toolkit for <em>C. bombi </em>will help us better understand how factors such as host diet, immune system, and physiology mediate outcomes of infection by these common parasites.</p>
Fig. 2. Multiplex PCR gel showing the 716 in Molecular diagnostics of the honey bee parasites Lotmaria passim and Crithidia spp. (Trypanosomatidae) using multiplex PCR
Fig. 2. Multiplex PCR gel showing the 716 to 724 bp amplicon for Lotmaria passim and Crithidia species, the L. passim specific 499 bp amplicon, and the Crithidia specific 245 bp amplicon.
Fig. 1 in Molecular diagnostics of the honey bee parasites Lotmaria passim and Crithidia spp. (Trypanosomatidae) using multiplex PCR
Fig. 1. Bayesian molecular phylogenetic tree showing relationship of 2 Hawaiian Lotmaria passim positive samples relative to other trypanosomes from Gen- Bank for a 608 bp region of the rDNA SSU gene.
Heather nectar extracts reduce within-colony epidemics of the bumblebee parasite <em>Crithidia bombi</em>
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Genetic modification of the bee parasite Crithidia bombi for improved visualization and protein localization
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Data for: Possible synergistic effects of thymol and nicotine against Crithidia bombi parasitism in bumble bees
<p>Data and R scripts for analysis of nicotine-thymol synergy experiment and thymol dose experiment evaluating effects of nectar secondary metabolite treatments on <em>Crithidia bombi </em>parasite load and mortality in the bumble bee <em>Bombus impatiens.</em></p>
Glyphosate Crithidia Publication Supporting Datasets and Analysis Straw and Brown
<p>The raw data, analysis and meta-data for each individual analysis within the publication. </p> <p>Files prior to revision</p>
Methods matter: the influence of method on infection estimates of the bumblebee parasite Crithidia bombi
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Data from: Temporal analysis of the honey bee microbiome reveals four novel viruses and seasonal prevalence of known viruses, Nosema, and Crithidia.
Honey bees (Apis mellifera) play a critical role in global food production as pollinators of numerous crops. Recently, honey bee populations in the United States, Canada, and Europe have suffered an unexplained increase in annual losses due to a phenomenon known as Colony Collapse Disorder (CCD). Epidemiological analysis of CCD is confounded by a relative dearth of bee pathogen field studies. To identify what constitutes an abnormal pathophysiological condition in a honey bee colony, it is critical to have characterized the spectrum of exogenous infectious agents in healthy hives over time. We conducted a prospective study of a large scale migratory bee keeping operation using high-frequency sampling paired with comprehensive molecular detection methods, including a custom microarray, qPCR, and ultra deep sequencing. We established seasonal incidence and abundance of known viruses, Nosema sp., Crithidia mellificae, and bacteria. Ultra deep sequence analysis further identified four novel RNA viruses, two of which were the most abundant observed components of the honey bee microbiome (∼10(11) viruses per honey bee). Our results demonstrate episodic viral incidence and distinct pathogen patterns between summer and winter time-points. Peak infection of common honey bee viruses and Nosema occurred in the summer, whereas levels of the trypanosomatid Crithidia mellificae and Lake Sinai virus 2, a novel virus, peaked in January.
Data from: Temporal analysis of the honey bee microbiome reveals four novel viruses and seasonal prevalence of known viruses, Nosema, and Crithidia.
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Temporal Analysis of the Honey Bee Microbiome Reveals Four Novel Viruses and Seasonal Prevalence of Known Viruses, Nosema, and Crithidia
GEO Series GSE28235. Fungi; unidentified entomopoxvirus; Iridovirus; Cypovirus; Luteovirus; Betabaculovirus; Gammabaculovirus; Birnaviridae; Apis mellifera; Black beetle virus; unclassified viruses; Densovirinae; unidentified baculovirus; Ascovirus. 363 samples. Type: Other.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.