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5 results for “Crithidia bombi”
Genetic modification of the bee parasite Crithidia bombi for improved visualization and protein localization
<p><em>Crithidia bombi </em>is a trypanosomatid parasite that infects several species of bumble bees (<em>Bombus</em> spp.), by adhering to their intestinal tract. <em>Crithidia bombi </em>infection impairs learning and reduces survival of workers and overwintering queens. Although there is extensive research on the ecology of this host-pathogen system, we understand far less about the mechanisms that mediate internal infection dynamics. <em>Crithidia bombi</em> infects hosts by attaching to the hindgut via the flagellum, and one previous study found that a nectar secondary compound removed the flagellum, preventing attachment. However, approaches that allow more detailed observation of parasite attachment and growth would allow us to better understand factors mediating this host-pathogen relationship. We established techniques for genetic manipulation and visualization of cultured <em>C. bombi.</em> Using constructs established for <em>Crithidia fasciculata, </em>we successfully generated <em>C. bombi </em>cells expressing ectopic fluorescent transgenes using two different selectable markers. To our knowledge, this is the first genetic modification of this species. We also introduced constructs that label the mitochondrion and nucleus of the parasite, showing that subcellular targeting signals can function across parasite species to highlight specific organelles. Finally, we visualized fluorescently tagged parasites <em>in vitro</em> in both their swimming and attached forms, and <em>in vivo </em>in bumble bee (<em>Bombus impatiens</em>) hosts. Expanding our cell and molecular toolkit for <em>C. bombi </em>will help us better understand how factors such as host diet, immune system, and physiology mediate outcomes of infection by these common parasites.</p>
Heather nectar extracts reduce within-colony epidemics of the bumblebee parasite <em>Crithidia bombi</em>
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Genetic modification of the bee parasite Crithidia bombi for improved visualization and protein localization
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Data for: Possible synergistic effects of thymol and nicotine against Crithidia bombi parasitism in bumble bees
<p>Data and R scripts for analysis of nicotine-thymol synergy experiment and thymol dose experiment evaluating effects of nectar secondary metabolite treatments on <em>Crithidia bombi </em>parasite load and mortality in the bumble bee <em>Bombus impatiens.</em></p>
Methods matter: the influence of method on infection estimates of the bumblebee parasite Crithidia bombi
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