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87 results for “Cryptosporidium”
Cryptosporidium GP60 Nested-PCR
<p>Video tutorial for<em> Cryptosporidium</em> spp. identification by nested-PCR on GP60 locus. Vision of <a href="https://zenodo.org/api/files/a60e7020-7da2-4f8c-856f-da3b5d0981b7/Cryptosporidium%20Nested-PCR%2018S%20rDNA_V2.mp4?versionId=4ac18f93-0faf-4b97-95f4-0df3b06739d7">Cryptosporidium Nested-PCR 18S rDNA_V2.mp4</a> is convenient for applying this tutorial.</p>
Fig. 2 in Host-adapted Cryptosporidium and Enterocytozoon bieneusi genotypes in straw-colored fruit bats in Nigeria
Fig. 2. Genotyping of Cryptosporidium spp. in strawcolored fruit bats by small subunit rRNA-based PCRRFLP. Upper panel: SspI RFLP patterns; lower panel: VspI RFLP patterns; M: 100-bp molecular markers; H: C. hominis positive control; P: C. parvum positive control; B1: Cryptosporidium bat genotype XIV; B2: Cryptosporidium bat genotype XV.
Fig. 1 in Cryptosporidium viatorum from the native Australian swamp rat Rattus lutreolus - An emerging zoonotic pathogen?
Fig. 1. Phylogenetic relationships of small subunit of nuclear ribosomal RNA (SSU) gene nucleotide sequence data (aligned over 563 bp) of selected Cryptosporidium taxa in relation to the novel C. viatorum genotype using the neighbor joining distance method. Individual GenBank accession numbers precede species name, followed by host common name and locality descriptors. Bootstrap support values (based on 2000 iterations) are indicated next to supported branches. Cryptosporidium baileyi was chosen as the outgroup. The novel genotype from this study is in bold-type. Scale bar indicates the number of nucleotide substitutions per site.
Fig. 4 in Host-adapted Cryptosporidium and Enterocytozoon bieneusi genotypes in straw-colored fruit bats in Nigeria
Fig. 4. Phylogeny of Enterocytozoon bieneusi genotypes in bats based on Bayesian inference analysis of sequences of the internal transcribed spacer of the rRNA gene. The posterior probability values are indicated on the branches. Red ones are E. bieneusi genotypes identified in straw-colored fruit bats in the present study. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Рис. 1. Ооцисты криптоспориΔий моΔифицированные, световая микроскопия по метоΔу ЦиΛя — НиΛьсена (Henriksen, Pohlenz 1981) (размеры увеΛичены в 1000 раз, 1 ΔеΛение равно 10 мкм): A — ооцисты изоΛированные из Testudo graeca из Апшеронской попуΛяции; B — ооцисты изоΛированные из Paralaudakia caucasia из Гобустанской попуΛяции; С — ооцисты изоΛированные из Eremias arguta; D — ооцисты изоΛированные из Natrix tessellata из Апшеронской попуΛяции. Автор: С. О. МамеΔова Fig. 1. Cryptosporidium oocysts stained with carbol-fucsin (Henriksen, Pohlenz 1981) (Magnification 1000 x, each segment corresponds to 10 μm): A — oocysts found in Testudo graeca from Absheron population; B — oocysts found in Paralaudakia caucasia from Gobustan population; C — oocysts found in Eremias argute; D — oocysts found in Natrix tessellata from Absheron population. Author: S. O. Mamedova in Intestinal coccidia (Apicomplexa: Coccidia) in reptiles of Azerbaijan and anthropogenic influences on their prevalence
Рис. 1. Ооцисты криптоспориΔий моΔифицированные, световая микроскопия по метоΔу ЦиΛя — НиΛьсена (Henriksen, Pohlenz 1981) (размеры увеΛичены в 1000 раз, 1 ΔеΛение равно 10 мкм): A — ооцисты изоΛированные из Testudo graeca из Апшеронской попуΛяции; B — ооцисты изоΛированные из Paralaudakia caucasia из Гобустанской попуΛяции; С — ооцисты изоΛированные из Eremias arguta; D — ооцисты изоΛированные из Natrix tessellata из Апшеронской попуΛяции. Автор: С. О. МамеΔова Fig. 1. Cryptosporidium oocysts stained with carbol-fucsin (Henriksen, Pohlenz 1981) (Magnification 1000 x, each segment corresponds to 10 μm): A — oocysts found in Testudo graeca from Absheron population; B — oocysts found in Paralaudakia caucasia from Gobustan population; C — oocysts found in Eremias argute; D — oocysts found in Natrix tessellata from Absheron population. Author: S. O. Mamedova
Fig. 1. Samples were identified within a in Diversity of Cryptosporidium in brush-tailed rock-wallabies (Petrogale penicillata) managed within a species recovery programme
Fig. 1. Samples were identified within a phylogenetic framework with the tree constructed using neighbour-joining with bootstrap test (1,000 replicates, displayed at nodes) using the 18S rRNA locus (878 bp). KV denotes Kangaroo Valley.
Fig. 2 in Elevation and vegetation determine Cryptosporidium oocyst shedding by yellow-bellied marmots (Marmota flaviventris) in the Sierra Nevada Mountains
Fig. 2. Association between Cryptosporidium oocyst shedding by yellow-bellied marmots in the Sierra Nevada Mountains and elevation (using 9,068 ft as the reference), stratified by presence or absence of vegetation at sites where scat samples were collected.
Fig. 1 in Elevation and vegetation determine Cryptosporidium oocyst shedding by yellow-bellied marmots (Marmota flaviventris) in the Sierra Nevada Mountains
Fig. 1. Locations in the Sierra Nevada Mountains where scat samples from yellow-bellied marmot were collected. The map contains 14 locations, but some locations clustered together due to the shorter distances between them and the scale of the map. The cluster in Yosemite National Park includes Tenaya Creek, Bridaveil Creek, Pothole Dome, and Lembert Dome. The cluster at Mineral King includes Mineral King, Eagle Lake, White Chief Creek, and Elk Kaweah River.
Fig. 2. Giardia duodenalis 18S in Giardia duodenalis and Cryptosporidium occurrence in Australian sea lions (Neophoca cinerea) exposed to varied levels of human interaction
Fig. 2. Giardia duodenalis 18S rRNA phylogenetic tree. Phylogenetic analysis of Giardia duodenalis positive samples was performed using a fragment of 18S rRNA gene. Analysis within the phylogenetic framework placed sea lion samples within the assemblage B (n = 27) and assemblage A clades (n = 1). Branch values indicate percent bootstrapping using 1000 replicates.
Fig. 1 in Giardia duodenalis and Cryptosporidium occurrence in Australian sea lions (Neophoca cinerea) exposed to varied levels of human interaction
Fig. 1. (A) Western Australia sampling locations. Faecal samples were collected from West Australia Sea lion colonies on Beagle and North Fisherman Islands. Coastal settlements and human impacted camping locations within close proximity to Sea lion colonies are indicated. (B) South Australia sampling locations. Australian sea lion faecal samples were collected from South Australia colonies; Blefuscu, Lewis, Liguanea, Lilliput, Olive and West Waldegrave Islands. Coastal towns and camping areas within close proximity to Australian Sea lion colonies are identified. (C) South Australia sampling locations: Kangaroo Island. Three colonies were sampled from Kangaroo Island including Cape Gantheaume, Seal Bay and Seal Slide. Coastal towns and recreational beach camping sites on the island are indicated.
Fig. 1 in Cryptosporidium rubeyi n. sp. (Apicomplexa: Cryptosporidiidae) in multiple Spermophilus ground squirrel species
Fig. 1. Cryptosporidium sp. Sbey11c oocysts from California ground squirrels (S. beecheyi). Differential interference contrast (DIC) microscopy (1000×), bar = 10 Mm.
Fig. 2 in Cryptosporidium rubeyi n. sp. (Apicomplexa: Cryptosporidiidae) in multiple Spermophilus ground squirrel species
Fig. 2. Phylogenetic relationships of partial 18S rRNA gene sequences of Cryptosporidium sp. Sbey11c from California ground squirrels (S. beecheyi) and other Cryptosporidium spp. inferred by neighbor-joining analysis with 1000 bootstrapping replicates.
Fig. 3 in Cryptosporidium rubeyi n. sp. (Apicomplexa: Cryptosporidiidae) in multiple Spermophilus ground squirrel species
Fig. 3. Phylogenetic relationships of partial actin gene sequences of Cryptosporidium sp. Sbey11c from California ground squirrels (S. beecheyi) and other Cryptosporidium spp. inferred by neighbor-joining analysis with 1000 bootstrapping replicates.
Fig. 1 in Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential
Fig. 1. Map of the studied area in the municipality of Mangaratiba, Rio de Janeiro State, Brazil. Red marker A — Collection site A; Red marker B — Collection site B; Green marker — The river known as "Rio do Saco" which leads to the ocean at collection site B. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).
Fig. 4 in Cryptosporidium rubeyi n. sp. (Apicomplexa: Cryptosporidiidae) in multiple Spermophilus ground squirrel species
Fig. 4. Phylogenetic relationships of partial HSP70 gene sequences of Cryptosporidium sp. Sbey11c from California ground squirrels (S. beecheyi) and other Cryptosporidium spp. inferred by neighbor-joining analysis with 1000 bootstrapping replicates.
Fig. 3 in Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China
Fig. 3. Maximum likelihood phylogenetic tree of Spirometra based on the cox1 gene. The tree was constructed with the cox1 sequences (444bp) by using the Kimura 2-parameter model with MEGA 7.0; we calculated bootstrap values with 1000 replicates. The representative sequence of Spirometra spagarnas isolated from snakes in this study are in bold print and marked by circles. Scale bar indicates nucleotide substitutions per site.
Fig. 1. A in Molecular characterization of Cryptosporidium and Enterocytozoon bieneusi in Père David's deer (Elaphurus davidianus) from Shishou, China
Fig. 1. A phylogeny tree of the ITS sequences with distances calculated by neighbor-joining analysis using the Kimura two-parameter model. Bootstrap values> 50% from 1,000 replicates are shown on the nodes. The genotypes identified in this study are shown as triangles.
Fig. 2 in Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China
Fig. 2. Maximum likelihood phylogenetic tree based on the 18S rRNA gene of Hepatozoon. The phylogenetic tree was constructed with the 18S rRNA gene sequences (670bp) by using the General time reversible model with MEGA 7.0; the bootstrap values were calculated with 1000 replicates. Representative sequences of Hepatozoon detected in this study are in bold print and marked by circles. Scale bar indicates nucleotide substitutions per site.
Fig. 1 in Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China
Fig. 1. Maximum likelihood phylogenetic tree based on the SSU gene of Cryptosporidium. The phylogenetic tree SSU gene (834bp) was constructed by using the Kimura 2-parameter model with MEGA 7.0 and the bootstrap values were calculated with 1000 replicates. Representative sequences of Cryptosporidium detected in snakes in this study are in bold print and marked by circles. Scale bar indicates nucleotide substitutions per site.
Fig. 1 in Prevalence and diversity of Cryptosporidium spp. in bamboo rats (Rhizomys sinensis) in South Central China
Fig. 1. Phylogenetic relationship of the five gene loci of Cryptosporidium species/genotypes. The Panel A to E are represent the actin, gp60, HSP70, COWP and SSU rDNA gene, respectively. The numbers on the branches are percent bootstrapping values from 1000 replicates. Each sequence is identified by its accession number and Cryptosporidium species/genotypes designation. The triangle filled in black indicate the subtypes identified in this study.
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