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96 results for “DOC”
Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) from river, lagoon, and open ocean sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple water types (river, lagoon, ocean) from the North Slope of Alaska and nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Water samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods and analyzed for dissolved organic carbon and total dissolved nitrogen content.
Total dissolved nitrogen (TDN), dissolved organic carbon (DOC), radiocarbon (14C-DOC), and stable carbon (13C-DOC) of surface waters from the Canning River watershed, 2019 and 2021
Sites along the Canning River mainstem and contributing streams near the Kavik River Camp, Alaska, were visited to track changes in stream and river total dissolved nitrogen (TDN) concentration, dissolved organic carbon (DOC) concentration, and the stable carbon (13C) and radiocarbon (14C) isotopic composition of DOC across transitions between the Brooks Range, Brooks foothills, and Arctic Coastal Plain. The dataset also includes water samples collected from lakes, springs, groundwater, and streams and rivers outside the Canning River watershed. Water samples were collected in late April and early August 2019 and in late July and early August 2021. Data include measurements of individual samples for TDN (milligrams nitrogen per liter), DOC (milligrams carbon per liter), carbon-13 of DOC (reported as delta-13C, per mil), carbon-14 of DOC (reported as fraction modern), and analytical error in the fraction modern values. Additional water chemistry data for these samples can be found in Koch et al. (2024). References: Koch, J. C., Connolly, C. T., Repasch, M., Best, H. R., Couvillion, C. S., Hunt, A. (2024). [Dataset] Hydrochemistry and age date tracers from springs, streams, and rivers in the Arctic National Wildlife Refuge, 2019-2022, U.S. Geological Survey data release, https://doi.org/10.5066/P95CXJIT.
Ramped Pyrolysis Oxidation (RPO) coupled radiocarbon (14C-DOC) and stable carbon (13C-DOC), high-resolution molecular composition (FT-ICR MS), and biodegradable dissolved organic carbon (BDOC) of groundwater, river water, and lagoon water in northeast Alaska, 2017
Supra-permafrost groundwater (SPGW), river water, and lagoon water were sampled near Kaktovik, AK to assess the reactivity and origin of dissolved organic matter (DOM) across interconnected hydrologic systems during late summer. Water samples were collected on August 17th 2017 from SPGW along the beach of Jago Lagoon (Jago GW), surface water from the Jago River’s main channel above tidal influence (Jago R), and from the water column of Kaktovik Lagoon at 2–3 m depth (KA LW). Measurements were made from grab samples for river and lagoon water, and from a composite sample for SPGW gathered from 10 individual shoreline locations. Data include dissolved organic carbon concentration (DOC, mg C L-1), Ramped Pyrolysis Oxidation (RPO) derived fraction compositions of 13C-DOC (δ13C ‰), 14C-DOC (in fraction modern), and method/instrumental error in the 14C and 13C results, and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) molecular composition and summarized compound classes. Biodegradable DOC (BDOC) bottle experiments were performed using all three sample types, where DOC concentration was subsequently measured at 2, 7, 14, and 28 days. FT-ICR MS composition was subsequently measured at the 28-day timepoint to track changes in molecular formulae and compound class relative abundance following biodegradation. Data from RPO serial thermal oxidation include temperature and normalized CO2 profiles for each background sample. Thermal-oxidation profiles of CO2 were transformed into non-parametric activation energy (E) distributions using an inverse model. Model output includes C mass of oxidized CO2 (µg C), Tmax (K), Emax (kJ mol-1), Emean (kJ mol-1), Estd (kJ mol-1), and p(0,E)max of user-defined sample fractions. FT-ICR MS results include a summary table of the relative abundance of compound classes (e.g., unsaturated phenolic, polyphenolic, aliphatic, condensed aromatics, peptide-like) and elemental groupings (e.g., CHO-type, CHON-type, CHOS-type, CHON
Sub-Alpine Lake (>600 m) High-Frequency Water Temperature, DOC (2007-2021), and Weather Station (Fall 2023) Dataset, Maine, USA.
We collected high-frequency surface and bottom water temperature in a set of nine high-elevation lakes in Maine, USA from 2007-2021. High-elevation is defined >600m above sea level. Dissolved organic carbon concentration data for the same time period and set of lakes is modified from Nelson, S.J., R.A. Hovel, J.F. Daly, A.L. Gavin, S. Dykema, and W.H. McDowell. 2021. Northeastern Mountain Ponds Geochemistry Compilation 1978-2019 ver 1. Environmental Data Initiative. https://doi.org/10.6073/pasta/8b51d651da0e0cff8c6ad853ef69ec3b. Air temperature and precipitation data were collected from a weather station deployed in the Mountain Pond watershed in Fall 2023 to aid comparison with low and high resolution PRISM datasets.
Cascade Project at North Temperate Lakes LTER Phosphorus, Chlorophyll, DOC, Color, and pH for Twenty UNDERC Lakes 1995 - 2003
Data on total phosphorous, chlorophyll a, dissolved organic carbon, water color, and pH for a set of lakes located at the University of Notre Dame Environmental Research Center (UNDERC). Surface water samples were collected monthly from May through August either from shore with a telescoping pole or from a boat. Twenty lakes were sampled from 1995-2000. Fifteen of these lakes were sampled from 2001-2003.
Water quality, temperature, ash-free dry mass, photosynthetic activate radiation (PAR), and zooplankton data from a warming and DOC subsidy experiment, 2020 - 2021.
This dataset includes chlorophyll-a concentrations, periphyton biomass estimates, water quality measurements, and qualitative observations from a large-scale mesocosm experiment conducted in the Green Lakes Watershed, Colorado. The experiment was designed to test how earlier lake ice-off and increased dissolved organic material (DOM), associated with terrestrial plant encroachment in alpine watersheds, interactively influence aquatic food webs. In fall 2019, twenty 2600L “megacosms” were established at Sandy Corner (3300 m ASL; 40.042289, -105.584006), left to fill with snowmelt, and maintained throughout the 2020 open water season. The experiment followed a 2 × 2 randomized block design manipulating ice-off timing (via black vs. beige tank coloration) and DOM inputs (presence/absence of willow leaf packs), with five replicates per treatment. All tanks were seeded with sediments and zooplankton from both alpine and montane lakes (Green Lake 1 and Green Lake 4), and instrumented with thermistors recording surface and hypolimnion temperature every two hours year-round. Periphyton growth was monitored using clay tiles, sampled across five time points. Chlorophyll-a concentrations were extracted from filtered water samples and analyzed spectrophotometrically. Periphyton biomass was estimated via ash-free dry mass (AFDM) determinations, based on the mass lost on combustion of material scraped from tiles. Water quality was measured 1–2 times weekly using a YSI ProPlus multiprobe and Li-Cor quantum sensor, and snow/ice cover was qualitatively assessed monthly during winter.
DIC and DOC 13C tracer data from Shark River Slough and Harney River (FCE), Everglades, South Florida in November 2011
As part of the larger NSF WSC funded project, an SF6 tracer study was conducted in November of 2011 by working groups form ENP, FIU and Univ. of Hawaii. This work included the release of the tracer (SF6), which was sampled in a lagrangian approach as the tracer “patch” moved from the fresh water portion of the system to the mangrove ecotone along the Shark and Harney Rivers. The goal of this study was to measure carbon export through the system. Annie Palya used our new CRDS system to measure the isotopic composition (d13C) of the DOM & DIC (analyzed at RSMAS) during this study, as carbon isotopes may help to resolve different sources of carbon. This analytical approach is relatively new, and will be important for understanding the C-dynamics within the FCE.
Dissolved Organic Carbon (DOC) and Dissolved Total Nitrogen (DTN) from Northeast U.S. Shelf Long Term Ecological Research (NES-LTER) Transect cruises, ongoing since 2022
Dissolved organic carbon and dissolved total nitrogen are measured from discrete bottle samples collected during CTD rosette casts on Northeast U.S. Shelf Long-Term Ecological Research (NES-LTER) transect cruises (ongoing since 2022). Sampling frequency is approximately seasonal. Sample collection is paired with particulate organic carbon at surface, subsurface chlorophyll max, and sometimes a third depth. Samples are filtered directly from the CTD rosette and acidified in the field, then analyzed using a Shimadzu TOC-LCPH total organic carbon analyzer coupled to a TNM-L analyzer for total nitrogen. Values are reported in micromoles per liter.
Accession numbers for radiocarbon and stable carbon isotopes of dissolved organic carbon (DOC) and dissolved inorganic carbon (DIC) in soil leachates from permafrost soils collected from the North Slope of Alaska in the summers of 2018 and 2022
Leachates of dissolved organic carbon (DOC) from permafrost soils were prepared from soils collected from the North Slope of Alaska in 2018 and 2022. Soil leachates were then either kept in the dark or exposed to light from LEDs at 305 nm (UV) and 405 nm (visible), and then inoculated with native microbial communities and incubated. At the start of the biological incubations, single replicates of the DOC after dark or light treatment and inoculation were assigned accession numbers and analyzed for 14C and 13C at the National Ocean Sciences Accelerator Mass Spectrometry (NOSAMS) facility. At the end of the biological incubations, duplicates of the dissolved inorganic carbon (DIC) in those waters were assigned accession numbers and analyzed for 14C and 13C at the NOSAMS facility.
Preparation of dissolved organic carbon (DOC) leachates from permafrost soils collected from the North Slope of Alaska in the summers of 2018 and 2022
Dissolved organic carbon (DOC) was leached from permafrost soils collected from the frozen permafrost layer at four sites underlying tussock tundra or wet sedge tundra vegetation and from both undisturbed soil and a thermokarst failure on the North Slope of Alaska during the summers of 2018 and 2022.
Radiocarbon and stable carbon isotopes of carbon dioxide produced by respiration of dissolved organic carbon (DOC) leached from permafrost soils collected from the North Slope of Alaska in the summers of 2018 and 2022
Dissolved organic carbon (DOC) was leached from permafrost soils near the Toolik Field Station in the Alaskan Arctic, either kept in the dark or exposed to light treatments, and then incubated with native permafrost microbial communities. The radiocarbon (14C) and stable carbon (13C) isotopic compositions of the initial DOC present in the dark or light-exposed permafrost soil leachates and the carbon dioxide (CO2) produced by microbial respiration of dark or light-exposed permafrost DOC were quantified.
Methane concentrations in dissolved organic carbon (DOC) leachates from permafrost soils collected from the North Slope of Alaska in the summers of 2018 and 2019
Methane (CH4) concentrations were measured in dissolved organic carbon (DOC) leachates of permafrost soils collected from the frozen permafrost layer at five sites underlying tussock tundra or wet sedge vegetation on the North Slope of Alaska during the summers of 2018 and 2019.
Monthly monitoring of Fluorescence, UV, Humic and non-Humic Carbon, Carbohydrates, and DOC for Shark River Slough, Taylor Slough, and Florida Bay, Everglades National Park (FCE LTER) for January 2002 to August 2004
A better understanding of the biogeochemical cycling of nutrients in the Florida Coastal Everglades is a key issue regarding the restoration of the Everglades, which is expected to change the water quality throughout South Florida. In addition to rain, the main freshwater supply to Florida Bay will be derived from Taylor Slough and the C-111 Basin in the north-east section of the Bay. While it is known that these areas deliver significant amounts of nitrogen to the Bay, a significant portion of this nitrogen is in its dissolved organic form (DON). The sources, environmental fate and bioavailability to microorganisms of this DON are however, not known. Preliminary data suggest that although proteins have been detected in canal samples, labile dissolved organic matter (DOM) components were found to increase in abundance in the freshwater marshes compared to their levels in the adjacent canal waters. Leaching experiments of biomass showed the presence of such labile DOM. However, this DOM was found to be susceptible to both biodegradation and photodecomposition. In this study we will focus on the determination of the molecular characteristics of both DOM and DON and assess the bioavailability of these materials in transects ranging from the C-111 canal and Taylor Slough to the central part of Florida Bay. Relevant water quality and spectroscopic parameters will be monitored at 11 sites on a monthly basis, while six of these sites will be sampled biannually for DOM and DON chemical characterization and bioavailability studies. Advanced analytical techniques such as pyrolysis-GC/MS, FTIR, 13C- and 15N-NMR, gel electrophoresis and LC/MS will be used in the molecular characterization effort. We envisage that this study will allow for a better assessment of the sources of DON and its bioavailability in this system.
Features of a FAIR vocabulary Supplementary docs
<p>Supplementary docs for FAIR vocabulary feature publication</p> <p>Content:</p> <p>ST1: The suitability of OBO principles used as FAIR Vocabulary Features</p> <p>ST2: FAIR Vocabulary Features mapped to FAIR principles and FAIR vocabulary requirements</p> <p>ST3: VersionIRI analysis</p> <p>ST4: RDA data maturity indicators that are not mapped to FAIR Vocabulary Features</p> <p>ST5: FAIR assessment results of Gene ontology</p> <p>ST6: FAIR assessment results of Experimental Factor Ontology</p> <p>ST7: FAIR assessment results of ICD-11</p>
Fleiss kappa for doc-2-doc relevance assessment
<p>Here we present a table summarizing the Fleiss’ kappa results. The Fleiss’ Kappa was calculated as a way to measure the degree of agreement between four annotators who evaluated the relevance of a set of documents (15 evaluation articles) regarding its corresponding “reference article”. The table contains 7 columns, the first one presents the topics, 8 in total. The second column shows the “reference articles”, represented by their PubMed-ID and organized by topic. The third column shows the Fleiss’ Kappa results. The fourth column shows the interpretation of the Fleiss' Kappa results being: i) “Poor” results <0.20, ii) “Fair” results within 0.21 - 0.40, and iii) “Moderate” results within 0.41 - 0.60. The fifth column shows the PubMed-IDs of evaluation articles rated by the four annotators as “Relevant” regarding its corresponding “reference article”. The sixth column shows the PubMed-IDs of evaluation articles rated by the four annotators as “Partially relevant” regarding its corresponding “reference article”. The seventh column shows the PubMed-IDs of evaluation articles rated by the four annotators as “Non-relevant” regarding its corresponding “reference article”.</p> <p> </p> <p><strong>Acknowledgements</strong> <br> This work is part of the STELLA project funded by DFG (project no. 407518790). This work was supported by the BMBF-funded de.NBI Cloud within the German Network for Bioinformatics Infrastructure (de.NBI) (031A532B, 031A533A, 031A533B, 031A534A, 031A535A, 031A537A, 031A537B, 031A537C, 031A537D, 031A538A).<br> </p>
Stable isotope and conservative tracer data used to estimate uptake of stream water dissolved organic carbon (DOC) through a whole-stream addition of a ¹³C-DOC tracer coupled with laboratory measurements of bioavailability of the tracer and stream water DOC using lability profiling with bioreactors
We performed a whole-stream addition of a ¹³C-DOC tracer and made laboratory measurements of the biological availability of the tracer as well as stream water DOC. The study was performed in October 2002 in a 1.27 km stretch of the third-order White Clay Creek in southeastern Pennsylvania. The tracer was prepared as a cold-water leachate of ¹³C-labeled tulip poplar saplings and it was added to the stream along with sodium bromide, a conservative tracer, over a 2-h period. Stream water samples were collected at 8 downstream stations over an 8-h period, filtered, and analyzed for concentrations of bromide and DOC. DOC was measured by Pt-catalyzed, persulfate oxidation, Br- was analyzed by ion chromatography, and C isotope samples were rotary evaporated, acidified, lyophilized, combusted, and the CO₂ analyzed with an elemental analyzer interfaced with an isotope ratio mass spectrometer. Lability profiling of the ¹³C-DOC tracer and stream water DOC were performed with a series of plug-flow bioreactors of increasing empty-bed contact times with the concentration of biodegradable DOC operationally defined as the difference between the DOC concentrations in the influent and effluent waters of the bioreactors. The bioreactor measurements were performed 2 days after the whole-stream release. Data were analyzed to estimate the uptake of stream water DOC associated with labile and semi-labile fraction of biodegradable DOC. These data have been previously used in a 2008 publication in Freshwater Biology, doi:10.1111/j.1365-2427.2007.01941.x.
Lake browning generates a spatiotemporal mismatch between DOC and limiting nutrients, 2018 spatial survey, modeled light limitation and whole-lake productivity changes in long-term Adirondack lake survey 1994-2012
This data set contains information on a spatial survey of dissolved organic matter (DOM) across lakes and wetlands in the Northeast and Midwest, USA and modeled long-term changes in light limitation and whole-lake productivity in a suite of lakes in the Adirondack State Park, New York, USA. Widespread long-term increases in DOM have been observed in many lakes in a process known as browning. This data set enables the assessment of potential changes in dissolved absorbance and dissolved organic nutrients associated with browning. This data set accompanies a manuscript in review at Limnology and Oceanography: Letters.
Photodegradation of carboxyl DOC from permafrost soils collected from the North Slope of Alaska in the summer of 2015
Dissolved organic carbon (DOC) was leached from permafrost soils near the Toolik Field Station in the Alaskan Arctic and then characterized for its photochemical properties. The photodegradation of carboxyl carbon (C) within permafrost DOC was quantified by 13C nuclear magnetic resonance (NMR).
Radiocarbon and stable carbon isotopes of CO2 produced from photomineralization of DOC leached from permafrost soils collected from the North Slope of Alaska in the summer of 2018
Dissolved organic carbon (DOC) was leached from permafrost soils near the Toolik Field Station in the Alaskan Arctic and then characterized for its photochemical properties. The radiocarbon (14C) and stable carbon (13C) isotopic compositions of carbon dioxide (CO2) photochemically produced from permafrost DOC were quantified.
Radiocarbon and stable carbon isotope dataset for DOC leached from permafrost soils collected from the North Slope of Alaska in the summer of 2018
Dissolved organic carbon (DOC) was leached from permafrost soils near the Toolik Field Station in the Alaskan Arctic. The radiocarbon (14C) and stable carbon (13C) isotopic compositions of permafrost DOC were quantified.
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