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105 results for “Diaporthe”
Activity of antioxidant enzymes and lipid peroxidation of soybean plants treated with five Diaporthe species
<p>Absorbance data from spectrophotometric measurements of catalase, reduced glutathion, lipid peroxidation and superoxide-dismutase of soybean cv. Sava plants infected with five <em>Diaporthe</em> species (i.e. <em>D. aspalathi</em>, <em>D. caulivora</em>, <em>D. eres</em>, <em>D. gulyae</em>, <em>D. longicolla</em>).</p> <p>Supplementary data to the publication Petrovic et al. (2023) The biochemical response of soybean cultivars infected by <em>Diaporthe</em> species complex. Plants 12, 2896. https://doi.org/10.3390/plants12162896</p>
The Genome Sequence of Citrus Melanose Pathogen Diaporthe citri and Two Citrus related Diaporthe species
<p>The melanose diseases is one the most widespread and economically important fungal diseases of citrus worldwide. The causative agent is filamentous fungus <em>Diaporthe citri</em> Wolf (syn. <em>Phomopsis citri</em> H.S. Fawc.). Here, we report genome assemblies of three strains of <em>D. citri</em>, namely strains ZJUD2, ZJUD14 and Q7, generated using a combination of PacBio Sequel long-read and Illumina paired-end sequencing data. The assembled genomes of <em>D. citri</em> ranged 53.97 Mb to 63.64 Mb in genome size, containing 15,977 ~ 16,622 protein-coding genes. In addition, we sequenced and annotated the genome sequences of two Citrus related <em>Diaporthe </em>species, including <em>D. citriasiana</em> and <em>D. citrichinensis</em>. The described genome sequences and annotations can provide a useful resource in the study of fungal biology, pathogen-host interaction, molecular diagnostic marker development, and population genomic analyses of Citrus-related <em>Diaporthe</em> species.</p>
FIGURE 2 in Diaporthe orixae sp. nov., an endophytic species isolated from Orixa japonica in southern China
FIGURE 2. Diaporthe orixae (HKAS 121465, holotype) a Host. b Stem of Orixa japonica. c, d Colonies on MEA. e Conidiomata. f, g Section through conidiomata. h, i Peridium. j–m Conidiogenous cells and conidia. n Alpha conidia. Scale bars: f = 50 µm, g = 100 µm, h–n = 10 µm.
FIGURE 1 in Diaporthe orixae sp. nov., an endophytic species isolated from Orixa japonica in southern China
FIGURE 1. Maximum likelihood tree generated by RAxML based on the combined ITS, tef1-α, β-tub, cal, and his3. Bootstrap support values for ML and MP equal to or greater than 70% and Bayesian posterior probabilities equal to or greater than 0.95 are given near nodes as ML/PP/MP, respectively. The tree was rooted with Diaporthella corylina (CBS 121124) and Diaporthella cryptica (CBS 140348). The newly generated strains are shown in red and the ex-type strains are in bold.
FIGURE 3 in Diaporthe orixae sp. nov., an endophytic species isolated from Orixa japonica in southern China
FIGURE 3. Diaporthe caryae (GZAAS 21–0393, new host record) a, b Colonies on MEA. c Immersed conidiomata. d Peridium. e Alpha and beta conidia f–i Conidiogenous cells. j, k Alpha conidia. l–n Beta conidia. Scale bars: d, e, l–n = 10 µm, f–k = 5 µm.
FIGURE 1 in A new host record of Diaporthe rosae on Rambutan (Nephelium lappaceum L.)
FIGURE 1. Consensus phylogram of 1,000 trees resulting from a RAxML analysis of the (ITS+Tef+TUB2+CAL) alignment of the analysed Diaporthe sp. sequences. RAxML bootstrap support values (ML above 60%), maximum parsimony (MP above 60%) and Bayesian inference (BYPP above 0.90) are given at the nodes (ML/MP/BYPP). The newly introduced sequences are in blue bold. The scale bar represents the expected number of changes per site. The tree is rooted to Valas ambiens CFCC 89894.
FIGURE 3 in A new host record of Diaporthe rosae on Rambutan (Nephelium lappaceum L.)
FIGURE 3. Diaporthe rosae (MFLUCC 22–0011) a Herbarium material. b–e Sporulation on PDA. Mycelium on PDA. g–h Conidiophores with Alpha-conidia. j–k Alpha-conidia. l–m Alpha-conidia and Beta–conidia. Scale bars: f, g, h, i = 10 µm, j, k, l, m = 5 µm.
FIGURE 5 in Two new endophytic fungi of Colletotrichum and Diaporthe isolated from Berchemia polyphylla var. leioclada in Guizhou Province, China
FIGURE 5. Phylogenetic tree based on RAxML analyses of a combined ITS, TUB2, CAL, HIS, TEF1-α dataset, including sequences of our strain and a selection of related Diaporthe spp., restricted according to the phylogenetic study of Supplementary Material (Figure S2). Diaporthe caulivora (CBS 127268) was used as the outgroup. The combined gene analysis included 13 strains with 2409 characters after aligned, including gaps (ITS: 560bp, TUB2: 526bp, CAL: 506bp, HIS3: 494bp, TEF1-α: 323bp). The RAxML analysis of the combined dataset yielded the best scoring tree with a final ML optimization likelihood value of -7651.550919. The matrix had 572 distinct alignment patterns, with 23.26% undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.203920, C = 0.334775, G = 0.247441, T = 0.213864; substitution rates AC = 1.486536, AG = 3.694646, AT = 1.459400, CG = 1.231075, CT = 5.843934, GT = 1.000000; gamma distribution shape parameter α = 0.257986. Bootstrap support values for RAxML equal to or greater than 70% and Bayesian posterior probabilities equal to or greater than 0.90 are given at each node. The new isolate is shown in red and bold.
FIGURE 4 in Two new endophytic fungi of Colletotrichum and Diaporthe isolated from Berchemia polyphylla var. leioclada in Guizhou Province, China
FIGURE 4. Diaporthe berchemiae (ex-type culture, GMBCC 20000). a, b Front and reverse colony on MEA. c, d Conidiomata on PDA. e–g Conidiophores. h–k Conidiogenous cells and conidia. l–o Alpha conidia. p-s Beta conidia. Scale bars: e–g, i–k = 10 μm; h, l–s = 5 μm.
FIGURE 2 in Two new endophytic fungi of Colletotrichum and Diaporthe isolated from Berchemia polyphylla var. leioclada in Guizhou Province, China
FIGURE 2. Phylogenetic tree of Maximum Likelihood analyses of Colletotrichum gloeosporioides species complex and its closely related species (including sequences of our strain and a selection of related Colletotrichum spp.,), restricted according to the phylogenetic study of Supplementary Material (Figure S1). Colletotrichum hippeastri (CBS 125376) and C. boninense CBS 123755 were used as outgroup taxa. The tree was built using concatenated sequences of ITS, ACT, CHS-1, GADPH, and TUB2 datasets and the tree topology of the RAxML and Bayesian analyses are similar. The combined gene analysis included 86 strains with 2152 characters after aligned, including gaps (ITS: 593bp, ACT: 282bp, CHS-1: 299bp, GADPH: 277bp, and TUB2: 701bp). The RAxML analysis of the combined dataset yielded the best scoring tree with a final ML optimization likelihood value of -15857.131621. The matrix had 1198 distinct alignment patterns, with 23.12% undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.229650, C = 0.299082, G = 0.241413, T = 0.229855; substitution rates AC = 1.009331, AG = 2.892663, AT = 1.227390, CG = 0.965860, CT = 4.527049, GT = 1.000000; gamma distribution shape parameter α = 0.449663. Bootstrap support values for RAxML equal to or greater than 70% and Bayesian posterior probabilities equal to or greater than 0.90 are given at each node. The new isolate is shown in red and bold.
FIGURE 1 in Two new endophytic fungi of Colletotrichum and Diaporthe isolated from Berchemia polyphylla var. leioclada in Guizhou Province, China
FIGURE 1. Colletotrichum berchemiae (ex-type culture, GMBCC 20001). a, b Front and reverse colony on PDA. c–e Conidiomata on PDA. h–j Conidiophores. k–m Conidiogenous cells and conidia. n–p Conidia. Scale bars: f = 100 μm; g–i, n = 20 μm, j, k = 30um, l, m, o, p = 10 um.
FIGURE 3 in Two new endophytic fungi of Colletotrichum and Diaporthe isolated from Berchemia polyphylla var. leioclada in Guizhou Province, China
FIGURE 3. Results of the PHI test of Colletotrichum berchemiae and closely related species using both LogDet transformation and splits decomposition. The PHI test results (Φw) <0.05 indicate significant recombination within the dataset. The new taxa are in red.
FIGURE 1 in Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China
FIGURE 1. Phylogram of Diaporthe based on combined ITS, CAL, HIS, TEF1-α and TUB2. MP and ML bootstrap support values above 75 % are shown at the first and second position. Thickened branches represent posterior probabilities above 0.95 from BI. Scale bar = 200 nucleotide substitutions. Ex-type strains are in bold. Strains in current study are in blue.
FIGURE 3 in Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China
FIGURE 3. Morphology of Diaporthe betulicola from Betula albosinensis (BJFC-S1333). A, B: Habit of conidiomata on twig. C: Transverse sections through conidiomata. D: Longitudinal sections through conidiomata. E: Conidiophores and conidia. F: Alpha conidia. G: Beta conidia. H: Colonies on PDA at 3 days (left) and 30 days (right). Scale bars: B–D = 500 μm; E–G = 10 μm.
FIGURE 2 in Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China
FIGURE 2. Morphology of Diaporthe betulae from Betula platyphylla (BJFC-S1317). A, B: Habit of conidiomata on twig. C: Transverse sections through conidiomata. D: Longitudinal sections through conidiomata. E: Conidiophores. F: Alpha conidia. G: Colonies on PDA at 3 days (left) and 30 days (right). Scale bars: B–D = 500 μm; E–F = 5 μm.
FIGURE 2 in Diaporthe species occurring on Senna bicapsularis in southern China, with descriptions of two new species
FIGURE 2. Morphology of Diaporthe sennae from Senna bicapsularis (BJFC-S1370). A, B: Habit of conidiomata on twig. C: Transverse sections through conidiomata. D: Longitudinal sections through conidiomata. E: Alpha and beta Conidia. F: Beta conidia. G: Colonies on PDA at 3 days (left) and 30 days (right). Scale bars: C–D = 200 μm; E–G = 5 μm.
FIGURE 1 in Diaporthe species occurring on Senna bicapsularis in southern China, with descriptions of two new species
FIGURE 1. Phylogram of Diaporthe based on combined ITS, CAL, HIS, TEF1-α and TUB. MP and ML bootstrap support values above 50 % are shown at the first and second position. Thickened branches represent posterior probabilities above 0.90 from BI. Scale bar = 90 nucleotide substitutions. Ex-type strains are in bold. Strains in current study are in blue.
FIGURE 3 in Diaporthe species occurring on Senna bicapsularis in southern China, with descriptions of two new species
FIGURE 3. Morphology of Diaporthe sennicola from Senna bicapsularis (BJFC-S1368). A: Habit of conidiomata on twig. B: Transverse sections through conidiomata. C: Longitudinal sections through conidiomata. D: Colonies on PDA at 3 days (left) and 30 days (right). E: Alpha conidia. F: Conidiophores. Scale bars: B–C = 200 μm; E–F = 5 μm.
FIGURE 3 in Phylogeny and morphology reveal two new species of Diaporthe from Traditional Chinese Medicine in Northeast China
FIGURE 3. Morphology of Diaporthe schisandrae from Schisandra chinensis (BJFC-S1370). A: Habit of conidiomata on branches. B: Longitudinal section of conidioma. C: Transverse section of conidioma. D, E: Conidia. F: Conidiophores. G: Colonies on PDA at 30 days. Scale bars: B–C = 200 μm; D–F = 10 μm.
FIGURE 2 in Phylogeny and morphology reveal two new species of Diaporthe from Traditional Chinese Medicine in Northeast China
FIGURE 2. Morphology of Diaporthe Sambucusii from Sambucus williamsii (BJFC-S1368). A, B: Habit of conidiomata on branches. C: Transverse section of conidioma. D, E, G: Conidia. F: Conidiophores. H: Colonies on PDA at 30 days. Scale bars: B–C = 500 μm; D, G = 5 μm; E–F = 10 μm.
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International Brain Laboratory public data
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OpenNeuro
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