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9 results for “DnaA”
Data from: Extrusion-modulated DnaA activity oscillations coordinate DNA replication with biomass growth
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Data from: Dispensability of extrinsic DnaA regulators in Escherichia coli cell-cycle control
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Revealing the roles of DnaA on the biological characteristics of Brucella and the inflammatory response to Brucella
<p>The <i>Brucella</i> cell cycle, including chromosomal replication and cell division, is coordinated with the virulence of the bacteria. DnaA, a chromosomal replication initiator protein, is an essential regulator of cell cycle progression and is tightly regulated both temporally and spatially because it is activated only once per cell cycle in bacteria. Although DnaA exists in many bacteria, the role of DnaA during <i>Brucella</i> infection, with the exception of its induction of the initiation of chromosome replication, remains unclear. Here, we generated a <i>Brucella</i> strain in which the expression of DnaA was conditionally controlled via anhydrotetracycline (ATc)-induced complementation of a deletion of the <i>dnaA </i>gene encoding the DnaA protein. We found that DnaA is required for <i>Brucella</i> division and activity to response various environmental stresses. As demonstrated by RNA-seq, DnaA was found to be involved in physiological processes, including the regulation of the cell cycle. Moreover, the inactivation of DnaA can inhibit <i>Brucella</i> intracellular trafficking through impaired replicative <i>Brucella</i>-containing vacuole (rBCV) biogenesis. In RAW 264.7 cells, the mutant strain promoted secretion of the proinflammatory cytokines interleukin 1β (IL-1β) and interleukin 6 (IL-6) and reduced survival. In addition, the inhibition of DnaA expression also attenuated the virulence of <i>Brucella</i>, but the resultant strain exhibited excellent immunogenicity to activate host immune responses in a mouse model. Altogether, these findings show that DnaA is involved in <i>Brucella</i> virulence in vitro and in vivo and could become a new target for the development of a safer and more efficient live attenuated <i>Brucella</i> vaccine.</p>
Laboratory Evolution Experiments Help Identify a Predominant Site of ΔrnhA-dnaA Constitutive Stable DNA Replication Initiation
GEO Series GSE135706. Escherichia coli str. K-12 substr. MG1655. 10 samples. Type: Expression profiling by high throughput sequencing.
Expression profiles of dnaA null, rok null, and dnaA null; rok null mutants
GEO Series GSE69788. Bacillus subtilis; Escherichia coli; Bacillus subtilis subsp. subtilis str. 168. 12 samples. Type: Expression profiling by array.
Expression profiles of Bacillus subtilis strains deleted for or ectopically expressing dnaA, sda, and spo0A during exponential growth and after treatment with HPUra.
GEO Series GSE84421. Bacillus subtilis; Escherichia coli; Bacillus subtilis subsp. subtilis str. 168. 58 samples. Type: Expression profiling by array.
Genome-wide map of Flagx3-DnaA bound to DNA in exponentially growing Sinorhizobium meliloti cultures
GEO Series GSE131423. Sinorhizobium meliloti. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
DnaA coordinates replication initiation and cell cycle transcription in Caulobacter crescentus
GEO Series GSE3171. Caulobacter vibrioides. 83 samples. Type: Expression profiling by array.
The impact of DnaA titration on Escherichia coli DNA replication initiation elucidated through live-cell single-particle tracking
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