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7 results for “Dothistroma septosporum”
Genetic architecture underlying response to the fungal pathogen Dothistroma septosporum in lodgepole pine, jack pine, and their hybrids
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Comparative gene expression analysis reveals mechanism of Pinus contorta response to the fungal pathogen Dothistroma septosporum
<p>Many conifers have distributions that span wide ranges in both biotic and abiotic conditions, but the basis of response to biotic stress has received much less attention than response to abiotic stress. In this study,<span> w</span>e investigated the gene expression response of lodgepole pine (<i>Pinus contorta</i>) to attack by the fungal pathogen <i>Dothistroma septosporum</i>, which causes <i>Dothistroma </i>needle blight (DNB), a disease that has caused severe climate-related outbreaks in northwestern British Columbia. We inoculated tolerant and susceptible pines with two <i>D. septosporum</i> isolates and analyzed the differentially expressed genes, differential exon usage, and co-expressed gene modules using RNA-seq data. We found a rapid and strong transcriptomic response in tolerant lodgepole pine samples inoculated with one <i>D. septosporum</i> isolate, and a late and weak response in susceptible samples inoculated with another isolate. We mapped 43 of the DEG- or gene-module-identified genes to the reference plant-pathogen interaction pathway deposited in KEGG database. These genes are present in PAMP-triggered and effector-triggered immunity pathways, including genes encoding mitogen-activated protein kinase and disease resistance protein. Genes comprising pathways and gene modules had signatures of strong selective constraint, while the highly expressed genes in tolerant samples appear to have been favored by selection to counterattack the pathogen. We identified candidate resistance genes that may respond to <i>D. septosporum</i> effectors. Taken together, our results show that gene expression response to <i>D. septosporum</i> infection in lodgepole pine varies both among tree genotypes and pathogen strains, and involves both known candidate genes and a number of genes with previously unknown functions.</p>
Data from: Planting exotic relatives has increased the threat posed by Dothistroma septosporum to the Caledonian pine populations of Scotland
To manage emerging forest diseases and prevent their occurrence in the future, it is essential to determine the origin(s) of the pathogens involved and identify the management practices that have ultimately caused disease problems. One such practice is the widespread planting of exotic tree species within the range of related native taxa. This can lead to emerging forest disease both by facilitating introduction of exotic pathogens, and by providing susceptible hosts on which epidemics of native pathogens can develop. We used microsatellite markers to determine the origins of the pathogen Dothistroma septosporum responsible for the current outbreak of Dothistroma needle blight (DNB) on native Caledonian Scots pine (Pinus sylvestris) populations in Scotland, and evaluated the role played by widespread planting of two exotic pine species in the development of the disease outbreak. We distinguished three races of D. septosporum in Scotland, one of low genetic diversity associated with introduced lodgepole pine (Pinus contorta), one of high diversity probably derived from the DNB epidemic on introduced Corsican pine (Pinus nigra subsp. laricio) in England, and a third of intermediate diversity apparently endemic on Caledonian Scots pine. These races differed for both growth rate and exudate production in culture. Planting of exotic pine stands in the UK appears to have facilitated the introduction of two exotic races of D. septosporum into Scotland which now pose a threat to native Caledonian pines both directly and through potential hybridisation and introgression with the endemic race. Our results indicate that both removal of exotic species from the vicinity of Caledonian pine populations, and restriction of movement of planting material are required to minimise the impact of the current DNB outbreak. They also demonstrate that planting exotic species that are related to native species reduces rather than enhances the resilience of forests to pathogens.
Comparative gene expression analysis reveals mechanism of Pinus contorta response to the fungal pathogen Dothistroma septosporum
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Data from: Planting exotic relatives has increased the threat posed by Dothistroma septosporum to the Caledonian pine populations of Scotland
Open the record for dataset details and reuse information.
Data from: Nested polymerase chain reaction–based detection of Dothistroma septosporum, red band needle blight of pine, a tool in support of phytosanitary regimes
Red band needle blight is one of the most important foliar diseases of Pinus species and is of increasing international forest health and biosecurity concern. To provide a rapid identification technique for this pathogen in support of official control measures, a nested polymerase chain reaction–based diagnostic assay that employs species-specific primer sets has been developed. The assay is able to detect the presence of the pathogen direct from pine needles, irrespective of host species, to within 10 fg of target DNA, the equivalent of approximately 2–3 ascospores or hyphael cells.
Data from: Nested polymerase chain reaction–based detection of Dothistroma septosporum, red band needle blight of pine, a tool in support of phytosanitary regimes
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