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27 results for “Drosophila serrata”
Drosophila serrata mutation accumulation lines: Phenotypic data on survival following infection with Drosophila C virus and reproduction
<p>The impact of selection on host immune function genes has been widely documented. However, it remains essentially unknown how mutation influences the quantitative immune traits that selection acts on. Applying a classical mutation accumulation (MA) experimental design in <em>Drosophila serrata</em>, we found the mutational variation in susceptibility (median time of death, LT50) to Drosophila C virus (DCV) was of similar magnitude to that reported for intrinsic survival traits. Mean LT50 did not change as mutations accumulated, suggesting no directional bias in mutational effects. Maintenance of genetic variance in immune function is hypothesised to be influenced by pleiotropic effects on immunity and other traits that contribute to fitness. To investigate this, we assayed female reproductive output for a subset of MA lines with relatively long or short survival times under DCV infection. Longer survival time tended to be associated with lower reproductive output, suggesting that mutations affecting susceptibility to DCV had pleiotropic effects on investment in reproductive fitness. Further studies are needed to uncover the general patterns of mutational effect on immune responses and other fitness traits, and to determine how selection might typically act on new mutations via their direct and pleiotropic effects.</p>
Drosophila serrata mutation accumulation lines: Phenotypic data on survival following infection with Drosophila C virus and reproduction
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Mating traits and fitness components in two breeding designs in Drosophila serrata
<p>Misalignment between male and female interests over mating creates inter-locus sexual conflict that is known to drive the coevolution of reproductive traits. Males and females also share the majority of their genome, which may cause these traits to genetically covary between the sexes and experience intra-locus sexual conflict where beneficial alleles in one sex are costly when expressed in the other. Traits expressed during mating may uniquely be subject to both intra- and interlocus sexual conflict. Here, we use a biologically repeated quantitative genetic experiment to test whether intra-locus sexual conflict is operating on mating latency, and copulation duration in the polyandrous fruit-fly Drosophila serrata. Mating latency and copulation duration were both heritable in males but not in females, and within sex and across-sex genetic correlations among the traits were all small. We found no significant genetic covariance between any measured mating behaviours and fitness components, nor between male and female fitness, suggesting that these traits are not under strong selection. Our study, therefore, finds little evidence of ongoing intra-locus sexual conflict over these traits. One explanation for our result is that these mating traits have historically been under strong selection, which has exhausted their genetic variation, making it difficult to detect selection in these populations. Keywords: mating latency, copulation duration, quantitative genetics, sexual conflict, Drosophila serrata, multivariate analyses, cuticula. </p>
Using inbreeding to test the contribution of non-additive genetic effects to additive genetic variance: A case study in Drosophila serrata
<p>Additive genetic variance, <em>V<sub>A</sub></em>, is the key parameter for predicting adaptive and neutral phenotypic evolution. Changes in demography (e.g., increased close-relative inbreeding) can alter <em>V<sub>A</sub></em>, but how depends on the, typically unknown, gene action and allele frequencies across many loci. For example, <em>V<sub>A</sub></em> increases proportionally with the inbreeding coefficient when allelic effects are additive, but larger (or smaller) increases can occur when allele frequencies are unequal at causal loci with dominance effects. Here, we describe an experimental approach to assess the potential for rare, recessive alleles to inflate <em>V<sub>A</sub></em> under inbreeding. Applying a powerful paired pedigree design in <em>Drosophila serrata</em>, we measured 11 wing traits on half-sibling families bred via either random or sibling mating, differing only in homozygosity (not allele frequency). Despite close inbreeding and substantial power to detect small <em>V<sub>A</sub></em>, we detected no deviation from the expected additive effect of inbreeding on genetic (co)variances. Our results suggest the average dominance coefficient is very small relative to the additive effect, or that allele frequencies are relatively equal at loci affecting wing traits. We outline the further opportunities for this paired pedigree approach to reveal the characteristics of <em>V<sub>A</sub></em>, providing insight into historical selection and future evolutionary potential.</p>
Using inbreeding to test the contribution of non-additive genetic effects to additive genetic variance: A case study in Drosophila serrata
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Mating traits and fitness components in two breeding designs in Drosophila serrata
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Data from: A genomic reference panel for Drosophila serrata
Here we describe a collection of re-sequenced inbred lines of Drosophila serrata, sampled from a natural population situated deep within the species endemic distribution in Brisbane, Australia. D. serrata is a member of the speciose montium group whose members inhabit much of south east Asia and has been well studied for aspects of climatic adaptation, sexual selection, sexual dimorphism, and mate recognition. We sequenced 110 lines that were inbred via 17-20 generations of full-sib mating at an average coverage of 23.5x with paired-end Illumina reads. 15,228,692 biallelic SNPs passed quality control after being called using the Joint Genotyper for Inbred Lines (JGIL). Inbreeding was highly effective and the average levels of residual heterozygosity (0.86%) were well below theoretical expectations. As expected, linkage disequilibrium decayed rapidly, with r2 dropping below 0.1 within 100 base pairs. With the exception of four closely related pairs of lines which may have been due to technical errors, there was no statistical support for population substructure. Consistent with other endemic populations of other Drosophila species, preliminary population genetic analyses revealed high nucleotide diversity and, on average, negative Tajima's D values. A preliminary GWAS was performed on a cuticular hydrocarbon trait, 2-MeC28 revealing 4 SNPs passing Bonferroni significance residing in or near genes. One gene Cht9 may be involved in the transport of CHCs from the site of production (oenocytes) to the cuticle. Our panel will facilitate broader population genomic and quantitative genetic studies of this species and serve as an important complement to existing D. melanogaster panels that can be used to test for the conservation of genetic architectures across the Drosophila genus.
Data from: The contribution of spontaneous mutations to thermal sensitivity curve variation in Drosophila serrata
Many traits studied in ecology and evolutionary biology change their expression in response to a continuously varying environmental factor. One well-studied example are thermal performance curves (TPCs); continuous reaction norms that describe the relationship between organismal performance and temperature and are useful for understanding the trade-offs involved in thermal adaptation. We assayed TPCs for locomotor activity in a set of 66 spontaneous mutation accumulation lines in the fly Drosophila serrata. Factor-analytic modeling of the mutational variance-covariance matrix, M, revealed support for three axes of mutational variation in males and two in females. These independent axes of mutational variance corresponded well to the major axes of TPC variation required for different types of thermal adaptation; 'faster-slower' representing changes in performance largely independent of temperature, and the 'hotter-colder' and 'generalist-specialist' axes, representing trade-offs. In contrast to its near-absence from standing variance in this species, a 'faster-slower' axis, accounted for most mutational variance (75% in males and 66% in females) suggesting selection may easily fix or remove these types of mutations in outbred populations. Axes resembling the 'hotter-colder' and 'generalist-specialist' modes of variation contributed less mutational variance but nonetheless point to an appreciable input of new mutations that may contribute to thermal adaptation.
Data from: Stronger convex (stabilizing) selection on homologous sexual display traits in females than in males: a multipopulation comparison in Drosophila serrata
Mutual mate choice for homologous sexual display traits has been demonstrated in Mutual mate choice for homologous sexual display traits has been demonstrated in several recent studies yet little attention has been given to quantitative comparison of the strength and form of mate preferences between the sexes. Such comparisons may provide important insight into the evolution of mate choice for honest signals. In particular, because females generally provide the majority of resources for initial offspring development, female displays may trade-off with fecundity, causing preference evolution to differ between the sexes. Recent theory suggests that adaptive male preferences for honest displays in females are possible under certain conditions and may result in preferences that are convex (i.e. stabilizing) in form. We compared sexual selection on a suite of contact pheromones arising from mutual mate choice using nine separate geographic populations of Drosophila serrata. We show that the convex selection is stronger on females than on males overall in these populations, and that convex selection is the predominate form of nonlinear selection on females but not males.
Quantifying and comparing male harm between two Drosophila serrata lab populations
<p>Male harm arises when traits that increase reproductive success in competition with other males also harm females as a side-effect. The extent of harm depends on male and female phenotypes, both of which can diverge between populations. Within a population, harm is inferred when increased exposure to males reduces female fitness, but studies of the divergence of male harm rarely manipulate male exposure. Here, we quantify male harm and compare its magnitude between two lab populations of <em>Drosophila serrata</em> that were derived from a common ancestor seven years earlier and subsequently held under conditions that minimized environmental differences. We manipulate female exposure to males in a factorial design involving all four combinations of males and females from these populations, providing insight into divergence in both sexes. Our results reveal substantial harm to females and provide stronger evidence of divergence in males than in females. Using these and other published data, we discuss conceptual issues surrounding the quantification and comparison of harm that arise because it involves a comparison of multiple quantities (e.g., female fitness under varying male exposure), and we demonstrate the increased insight that is gained by manipulating male exposure to quantify these.</p>
The genetic and social contributions to sex differences in lifespan in Drosophila serrata
<p class="MsoNormal">Sex differences in lifespan remain an intriguing puzzle in evolutionary biology. While explanations range from sex differences in selection to sex differences in the expression of recessive lifespan-altering mutations (via X-linkage), little consensus has been reached. One unresolved issue is the extent to which genetic influences on lifespan dimorphism are modulated by the environment. For example, studies have shown that sex-differences in lifespan can either increase or decrease depending upon the social environment. Here we took an experimental approach, manipulating multiple axes of social environment across inbred long- and short-lived genotypes and their reciprocal F1s in the fly <em>Drosophila serrata</em>. Our results reveal strong genetic effects and subtle yet significant genotype-by-environment interactions for male and female lifespan, specifically due to both population density and mating status. Further, our data do not support the idea that unconditional expression of deleterious X-linked recessive alleles in heterogametic males accounts for lower male lifespan.</p>
Data from: The contribution of spontaneous mutations to thermal sensitivity curve variation in Drosophila serrata
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Data from: Comparing the intersex genetic correlation for fitness across novel environments in the fruit fly, Drosophila serrata
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Quantifying and comparing male harm between two Drosophila serrata lab populations
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Data from: Stronger convex (stabilizing) selection on homologous sexual display traits in females than in males: a multipopulation comparison in Drosophila serrata
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The genetic and social contributions to sex differences in lifespan in Drosophila serrata
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Data from: A genomic reference panel for Drosophila serrata
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Data from: Condition dependence of a multicomponent sexual display trait in Drosophila serrata
Theory predicts that costly sexual displays should evolve condition dependence if the marginal fitness gain from trait exaggeration is greater for high- than for low-condition individuals and that the strength of condition dependence should increase with the strength of directional selection. While there is substantial support for the first prediction, evidence for the latter is much weaker. We undertook a quantitative test of this prediction for a multivariate sexual display consisting of a suite of contact pheromones termed "cuticular hydrocarbons" (CHCs) in Drosophila serrata. We performed a dietary manipulation of condition (i.e., the pool of metabolic resources available for allocation to fitness-enhancing traits) within a half-sibling breeding design, thereby also providing insight into the genetic basis of condition dependence. As predicted, the linear combination of CHCs under the strongest sexual selection from female mate preferences was unusually condition dependent relative to other CHC combinations within the population (P = .049). A significant positive correlation also existed between the strengths of condition dependence and sexual selection among different CHC blends (r = 0.482, P < .001). Finally, sires varied in their response to the dietary manipulation, demonstrating significant genetic variance in condition dependence. Our results are consistent with the evolution of heightened condition dependence of sexual displays in response to persistent sexual selection.
Data from: Territory defense as a condition-dependent component of male reproductive success in Drosophila serrata
Sexual selection arises from both intrasexual competition and mate choice. With respect to the evolution of male traits, there is a vast literature documenting the existence of female choice and male-male competition, and both have been shown to co-occur in many species. Despite numerous studies of these two components of male reproductive success in isolation, few have investigated whether and how they interact to determine total sexual selection. To address this, we investigate male territoriality in Drosophila serrata, a species in which female preference for male sexual pheromones (cuticular hydrocarbons or CHCs) have been extensively studied. We demonstrate that territoriality occurs, that it involves direct male-male aggressive interactions, and that it contributes to variation in male mating success. Results from a phenotypic manipulation also indicate that territorial success is condition-dependent, although a genetic manipulation of condition, involving three generations of full-sib inbreeding, failed to find a significant effect. Finally, selection assays also suggest that territorial success depends on male body size but not on CHCs, whereas the opposite is true for mating success.
Data from: Crowd control: sex ratio affects sexually selected cuticular hydrocarbons in male Drosophila serrata
Although it is advantageous for males to express costly sexually selected signals when females are present, they may also benefit from suppressing these signals to avoid costly interactions with rival males. Cuticular chemical profiles frequently function as insect sexual signals; however, few studies have asked whether males alter these signals in response to their social environment. In Drosophila serrata, an Australian fly, there is sexual selection for a multivariate combination of male cuticular hydrocarbons (CHCs). Here, we show that the ratio of females to males that an adult male experiences has a strong effect on his CHC expression, with female-biased adult sex ratios eliciting greater expression of CHC profiles associated with higher male mating success. Classical models predict that male reproductive investment should be highest when there is a small but nonzero number of rivals, but we found that males expressed the most attractive combination of CHCs when there were no rivals. We found that male CHCs were highly sensitive to adult sex ratio, with males expressing higher values of CHC profiles associated with greater mating success as the ratio of females to males increased. Moreover, sex ratio has a stronger effect on male CHC expression than adult density. Finally, we explore whether sex ratio affects the variance among a group of males in their CHC expression, as might be expected if individuals respond differently to a given social environment, but find little effect. Our results reveal that subtle differences in social environment can induce plasticity in male chemical signal expression.
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