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81 results for “ELISA”
Metrics As Scores Dataset: Elisa Spectrophotometer Positive Samples
<p>The ELISA dataset contains data from a spectrophotometer that determined the optical density of positive control samples, from five different lots, across five different runs. This dataset was introduced by (Schmid 1991).</p> <p>This dataset has the following <strong>Features</strong>:</p> <ul> <li><em>Lot1</em>: The first lot</li> <li><em>Lot2</em>: The second lot</li> <li><em>Lot3</em>: The third lot</li> <li><em>Lot4</em>: The fourth lot</li> <li><em>Lot5</em>: The fifth lot</li> </ul> <p>It has a total of 5 <strong>Groups</strong>: <em>Run1</em>, <em>Run2</em>, <em>Run3</em>, <em>Run4</em>, and <em>Run5</em>.</p>
In-house CHO HCP Platform: a promising approach for HCP ELISA monitoring
Open the record for dataset details and reuse information.
A copro-antigen ELISA for the detection of ascarid infections in chickens
<p>Dataset for the infection experiments to evaluate the performance of copro-antigen ELISA to assess nematode infections. Chickens were divided into three groups. One group was kept as uninfected control, and the birds of the other two groups were either experimentally infected with 100 or 1,000 embryonated/infective eggs of either <em>Ascaridia galli</em> or <em>Heterakis gallinarum</em></p>
Figure 1 in Identification of blood meals in field collected Culex pipiens, Anopheles sacharovi and Culex tritaeniorhynchus (Diptera: Culicidae) using the ELISA method
Figure 1. Sampling localities of Anopheles sacharovi, Culex pipiens, Culex tritaeniorhynchus populations (1. Huzurkent, 2. Düziçi, 3. Akhisar, 4. Dalaman, 5. Gelendost, 6. Selçuk, 7. Karataş, 8. Eşme, 9. Türkoğlu, 10. Dörtyol, 11. Kırıkhan, 12. Manavgat, 13. Afyon, 14. Tarsus, 15. Kadirli, 16. Aydın, 17. Kozan, 18. Sandıklı, 19. Dinar, 20. Uşak, 21. Ceyhan, 22. Antalya, 23. Tuzla 24. İzmir, 25. Söke, 26. Kuşadası, 27. Akköy). Red stars indicate locations where Cx. pipiens was sampled, the green diamond shape indicates locations of Cx. tritaeniorhynchus and the blue pins indicate the locations of An. sacharovi.
Figure 2 in Identification of blood meals in field collected Culex pipiens, Anopheles sacharovi and Culex tritaeniorhynchus (Diptera: Culicidae) using the ELISA method
Figure 2. Percentage distributions of single and multiple host meal choices for three mosquito species collected in the Aegean and Mediterranean regions.
Fig. 6 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)
Fig. 6. Analysis of the sensitivity and specificity of the P. relictum MSP-1 capture antigen-based ELISA. Serial dilutions of normal chicken serum and three sera samples from P. relictum-infected penguins (8776, 8783 and 8784) were used for coating the assay wells. Each dilution was performed in triplicate, and the data shown represent means of three independent experiments with standard error bars and levels of statistical significance (****: P <0.0001).
Fig. 4 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)
Fig. 4. Validation of ELISA. The ELISA developed using P. relictum MSP-1 protein was tested with known P. relictum positive penguin serum collected from penguin # 8790 at week 26. Black column represents positive penguin serum. Hatched and white columns represent negative controls containing normal chicken serum and PBS, respectively. Grey column represents the positive reaction containing streptavidin alkaline-phosphatase and its substrate pnitrophenyl phosphate. Each reaction was performed in triplicate, and the data shown represent means of three independent experiments with standard error bars and levels of statistical significance (****: P <0.0001).
Fig. 2 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)
Fig. 2. Verification of the biotinylation of recombinant P. relictum MSP-1. Purified recombinant P. relictum MSP-1 protein was labeled with biotin. Biotinylation of MSP-1 was confirmed using an enzyme (streptavidin alkaline phosphatase)-linking assay and absorbance read at 405 nm indicated the presence of biotinylated MSP-1. Black column represents biotinylated MSP-1 protein. Hatched column and white column represent negative controls containing non-biotinylated MSP-1 and PBS, respectively. Grey column represents the positive control containing streptavidin alkaline-phosphatase (SAP) and its substrate p-nitrophenyl phosphate (PNPP). Each reaction was performed in triplicate, and the data shown represent means of three independent experiments with standard error bars and levels of statistical significance (****: P <0.0001).
Fig. 3 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)
Fig. 3. Biotin-labeled MSP-1 protein titration curve. Serially diluted (300 ng/ μL to 0.003 ng/μL) biotinylated MSP-1 was used for coating the surfaces of the reaction wells overnight at 4 ◦C. The amount of biotinylated MSP-1 immobilized on the surface of the well was proportional to the intensity of the colored product generated which in turn was proportional to the absorbance value measured at 405 nm wavelength. Reactions were performed in triplicate, and the data shown represent means of three independent experiments with standard error bars.
Fig. 7. MSP-1 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)
Fig. 7. MSP-1 capture antigen-based ELISA analysis of 370 sera samples collected from 11 penguins during three consecutive seasons of Spring (March to May), Summer (June to August) and Fall (September to November). (A) ELISA absorbance readings for the 370 sera samples collected from Spring to Fall are arranged in ascending order. Time of collection is indicated by color. Light grey represents Spring, dark grey represents Summer, and black represents Fall. Black dashed line indicates the single cut-off point (0.488) determined by change-point analysis. (B) Left: percentage of positive (A405 ≥ 0.488) and negative (A405 <0.488) sera samples; Right: distribution of positive samples in Spring (March to May), Summer (June to August), and Fall (September to November). (C) Percentage of penguin sera samples that tested positive (A405> = 0.488) in each month from March to November.
Fig. 5. P. relictum MSP-1 in Plasmodium relictum MSP-1 capture antigen-based ELISA for detection of avian malaria antibodies in African penguins (Spheniscus demersus)
Fig. 5. P. relictum MSP-1 capture antigen-based ELISA analysis of test sera from penguins. Sera samples (370 total) from eleven penguins collected from Spring through Fall season were used as test samples in the assay to determine anti-P. relictum antibodies level. Light grey, dark grey, and black columns represent penguin sera samples collected in Spring, Summer, and Fall, respectively. Panel A–K represents ELISA results for individual penguins' sera collected at different time points from Spring to Fall. Panel L represents average ELISA absorbances for all 11 penguins at different sampling points. Each sample was assayed in triplicate, and the data shown represent means of three independent experiments with standard error bars.
Fig. 1 in Comparison of a commercial ELISA and indirect hemagglutination assay with the modified agglutination test for detection of Toxoplasma gondii antibodies in giant panda (Ailuropoda melanoleuca)
Fig. 1. Receiver operating characteristics (ROC) analysis of the ELISA. ROC analysis shows an area under the curve (AUC) of 0.861 (95% CI: 0.712–1.000) for ELISA (a), 0.894 (95% CI: 0.791–0.997) for ELISA (b), and 0.902 (95% CI: 0.799–1.000) for ELISA (c).
ELISA MASRIERA - Francesc Masriera Manovens
CAT: Retrat funerari que Francesc Masriera va fer de la seva germana Elisa (1883). L'obra va ser donada per Lluís Masriera el 1947 al Museu de l'Empordà, que és on es custodia i es pot visitar actualment. Dimensions: 38 x 72 cm. ESP: Retrato funerario que Francesc Masriera hizo de su hermana Elisa (1883). La obra fue donada por Lluís Masriera en 1947 al Museo del Empordà, que es donde se custodia y se puede visitar actualmente. Dimensiones: 38 x 72 cm. ENG: Portrait that Francesc Masriera painted of his sister, Elisa (1883). In 1947 Lluís Masriera donated it to the Museum of the Empordà where it is now on display. Dimensions: 38 x 72 cm. FRA: Portrait funéraire que Francesc Masriera a fait de sa sœur Elisa (1883). L'œuvre a été offerte par Lluís Masriera en 1947 au Museu de l'Empordà, où elle est actuellement conservée et peut être visitée. Dimensions: 38 x 72 cm. Model creat per Aleix Barberà Giné i Luz Gamiz (text) en el marc del projecte Giravolt: https://bit.ly/3CWm6L2 Source: Objaverse 1.0 / Sketchfab
Development of a genus-specific antigen capture ELISA for orthopoxviruses. Target selection and optimized screening
<p><strong>Raw data for quantification of anti surface protein antibody binding to vaccinia virus.</strong></p> <p>Method description</p> <p>Immuno-negative staining and electron microscopy were performed as described elsewhere (Laue, 2010). Briefly, purified VACV<sub>NYCBOH</sub> particles were inactivated by incubation in freshly prepared 2% PFA in 0.05 M HEPES (pH 7.2), sonicated and immobilized on sample supports for transmission electron microscopy. Biotinylated pAbs were titrated on BSA coated grids, until detection with 5 nm gold nanoparticle coupled streptavidin (British Biocell, Cardiff, United Kingdom) resulted in the same mean background labelling density of ~10 particles per view field at a, 87,000-fold magnification (anti-A27: 0.7 µg/mL; anti-D8: 2 µg/mL; anti-H3: 0.9 µg/mL; anti-L1: 1.9 µg/mL). Negative staining was performed with either 0.1 or 0.5% uranyl acetate solution. For quantification, only IMV particles of the mulberry form, which were found isolated from other particles, were analyzed. Randomized sampling was done in 22 evenly distributed mesh areas with five viral particles analyzed per area. Imaging was done with a Tecnai 12 BioTwin (FEI Corp.) at 120 kV and a 1k digital CCD camera (Megaview III, Olympus Soft Imaging Solutions).</p>
Tissue factor content measured by ELISA in different size fractions of EVs.
<p>Figure 1. Tissue factor (TF) content measured by ELISA in different size fractions of EVs.</p> <p>Data expressed as pg of TF in 1 x 10<sup>6</sup> EV ± SD from eight independent experiments. Data analyzed by one-way ANOVA, * symbolizes statistical significance p<0.05. <1000nm, <450nm, <200nm indicate samples filtered using syringe-top filters and their respective pore sizes.</p>
Source ELISA data for the manuscript "Restrained expansion of the recall germinal center response as biomarker of protection for influenza vaccination in mice"
<p>This repository contains the source ELISA data for the manuscript "Restrained expansion of the recall germinal center response as biomarker of protection for influenza vaccination in mice" currently under review by PLOS ONE.</p> <p>It supports the following figures:</p> <p>Fig 4A: rHA ELISA data miniHA study.xlsx<br> Fig 4B: Competition ELISA data miniHA study.xlsx<br> S7 Fig: Competition ELISA data POC study.xlsx</p> <p> </p> <p>Files include Raw OD's per plate and reported values analysis. </p> <p> </p>
The use of nanobodies in a sensitive ELISA test for SARS-CoV-2 Spike 1 protein
<p>A rapid detection method for SARS-CoV-2 spike protein is essential for control of COVID19. We investigated various combinations of engineered nanobodies in a sandwich ELISA to detect the Spike protein of SARS-CoV-2. We have identified an optimal combination of nanobodies. These were selectively functionalised to further improve antigen capture. This dataset contains data from ELISA experiments described in the manuscript.<span> </span></p> <p><span>Plate coating of nanobodies for ELISA by passive adsorption vs biotinylation was compared. A series of nanobody pairings (two cluster 2 ACE2-binding epitope and two cluster 1 CR3022 epitope) were screened for optimum sensitivity. The optimal pair were then tested against a series of SARS-COV-2 antigens: recombinant spike 1 protein; recombinant receceptor binding domain (RBD); pseudotyped HIV-1 and heat-empigen inactivated SARS-CoV-2 virus. X-ray irradiated SARS-CoV-2 was also tested. Sensitivity to these antigens was compared with nanobodies biotinylated a) site-selectively and b) in a non-specific stochastic manner. Batch-to-batch viral variation and effects of inactivating agents were investigated. Limit of detection was compared against delta and beta viral mutants. Combining optimal nanobody pairing and site-selective biotinylation, we observed a limit of detection of 147 pg/mL for Spike protein; 33 pg/mL for RBD; 16 TCID50/mL of pseudovirus and 15 ffu/mL of heat-Empigen inactivated SARS-CoV-2. The pairing also showed sensitivity towards delta variant. We have demonstrated the use and sensitivity of nanobodies in ELISA by detection of recombinant and viral SARS-CoV-2 antigens.</span></p>
Supplementary Figures -B Cell Epitopes Mapping of the Vibrio cholera Toxins A, B, and P and an ELISA assay
<p>Figure S1- List of <em>Vibrio cholerae</em> [toxin A (P01555), B (P01556), and P (P29485)] synthetic peptides and position in the cellulose membrane of Spot synthesis.</p> <p>Figure S2- Purification of the peptide B (Vc/TxB-11) by HPLC using an XBridge BEH C18 (2.7 μ, 5 cm x 4.6 mm) column coupled to a Water HPLC system at a flow rate of 1.2 ml min<sup>-1 </sup>using mobile phases A [0.05% formic acid in water (18 MΩ × cm)] and B [(0.05% formic acid in ACN (acetonitrile acid)] (v/v) in water. Detection at 200-300 nm using a diode array.</p> <p>Figure S3- Mass spectrometry. The peptide B (Vc/TxB-11) was solubilized in deionized water to a final concentration of 10 µg/ml and then added formic acid to a final concentration of 0.1%. The mass spectrometer used was the Water UPLC model Acquity-I Class. The samples were electronically injected by the equipment at 1 µl/min. The range used for ion detection ranged from 1000-11500 m/z.</p>
DMID14-0100: A randomized, controlled Phase 1b trial of the Sm-TSP-2 vaccine for intestinal schistosomiasis ELISA results
<p>Recombinant <em>Schistosoma</em> <em>mansoni</em> Tetraspanin-2 formulated on Alhydrogel (<em>Sm</em>-TSP-2/Alhydrogel) is being developed to prevent intestinal and hepatic disease caused by <em>S</em>. <em>mansoni</em>. The tegumentary <em>Sm</em>-TSP-2 antigen was selected based on its unique recognition by cytophilic antibodies in putatively immune individuals living in areas of ongoing <em>S</em>. <em>mansoni</em> transmission in Brazil, and preclinical studies in which vaccination with <em>Sm</em>-TSP-2 protected mice following infection challenge. </p>
In conversation with Elisa Citterio
<p>In questo video, il Maestro Elisa Citterio, del corso Creative Associates, racconta la sua esperienza all'Accademia Stauffer, insegnando agli allievi il valore e la bellezza della musica barocca.</p> <p>Il video è stato realizzato dal responsabile Media dell'Accademia Stauffer Giuseppe Milanese.</p>
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