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116 results for “Embryo culture”
Comparison Of The Embryoscope Time-Lapse System With Standard Embryo Culture
ClinicalTrials.gov study NCT02246309. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Automation of Gamete Preparation, Intracytoplasmic Sperm Injection (ICSI), Embryo Culture, and Vitrification
ClinicalTrials.gov study NCT06074835. IPD Sharing: YES. Countries: 1. Publications: 1.
Optimized culture systems for the preimplatation ICR mouse embryos with wide range of EDTA concentrations
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Effect of hexanediol treatment on clusters of RNA polymerase II in zebrafish embryo primary cell culture
<p>This data set assesses changes in RNA polymerase II clusters upon treatment with 3% 1,6-hexanediol in zebrafish embryos. RNA polymerase was labeled by immunofluorescence, microscopy images were acquired by instant-SIM microscopy and analyzed using MatLab scripts and the bioformats importer. This data set contains the raw image data as well as all further analysis scripts.</p>
Microscopy images of the DNA and RNA polymerase II distribution inside nuclei of cell cultures obtained from pluripotent zebrafish embryos
<p><strong>Image data description</strong></p> <p>Color channels in the image data:</p> <ol> <li>First channel: DNA (Hoechst 33342)</li> <li>Second channel: Pol II Ser2P (Elongating RNA polymerase II, indirect immunofluorescence, STAR RED)</li> <li>Third channel: Pol II Ser5P (Recruited RNA polymerase II, indirect immunofluorescence, Alexa 594)</li> </ol> <p><strong>Sample description</strong></p> <p>Praimary cell cultures were prepared from pluripotent zebrafish embryos were collected at the sphere stage of development, treated with chemical inhibitors of transcription (Control, 500 mM Flavopiridol, 500 mM Triptolide, all for 30 min) and fixed overnight (2% formaldehyde in culutre media, 30 min, room temperature; followed by 8% formaldehyde for 15 min at room temperature). RNA polymerase in the recruited state (Pol II Ser5P) and the elongating state (Pol II Ser2P) were labeled by indirect immunofluorescence (permeabilization 0.5% Triton X-100 in PBS 15 min room temperature, 30 min blocking 4% BSA in PBST, rat IgG anti-Pol II Ser5P & rabbit IgG anti-Pol II Ser2P in 4% BSA in PBST overnight 4°C, anti-rat Alexa 594 & anti-rabbit STAR RED in 4% BSA in PBST overnight 4°C). Mounted in VectaShield H-1000 with 2 µM Hoechst 33342 added for fluorescent DNA labeling. Scan of lab book page is included in the repository.</p> <p>The data set contains images obtained from two samples for each condition.</p> <p><strong>Imaging</strong></p> <p>Microscopy images acquired using VisiTech iSIM with dual camera setup. Objective Nikon CFI SR HP Apo<br> TIRF 100XAC Oil, color channels acquired in a sequence to reduce overlap (DNA + Ser2P acquired simultaneously on two camerase, Ser5P acquired after on a single camera), z-stack settings optimized to ensure reliable xyz alignment of channels. Imags were cropped to the region with best signal and resolution in the DNA channel, same region used throughout the entire dataset. all imaging settings were kept unchanged over the course of acquisition, all images acquired in a single session of 4 hours.</p> <p><strong>Advice for image processing</strong></p> <p>Images can be loaded for processing with the OME bioformats importer. An import script for MatLab is available through the Hilbert lab: https://github.com/lhilbert/NuclearObjects_ImageAnalysis</p> <p><strong>Author contributions</strong></p> <p>AN & MS provided embryos, carried out cell culture, and prepared samples, LH performed microscopy</p>
Exosome-Enriched Culture Media to Enhance In Vitro Maturation and Embryo Development in Poor Ovarian Responders Undergoing IVF
ClinicalTrials.gov study NCT07085312. IPD Sharing: NO. Countries: 1. Publications: 2.
Embryo Culture At a Constant Temperature of 36.6°C or 37.1°C
ClinicalTrials.gov study NCT03548532. IPD Sharing: YES. Countries: 1. Publications: 1.
Cell-free DNA Analysis of Spent Embryo Culture Media as a Non-invasive Approach for Preimplantation Genetic Diagnosis
ClinicalTrials.gov study NCT07076719. IPD Sharing: UNDECIDED. Countries: 1. Publications: 24.
Time-Lapse Incubation for Embryo Culture - Morphokinetics and Environmental Stability
ClinicalTrials.gov study NCT02657811. IPD Sharing: Not stated. Countries: 1. Publications: 26.
Targeted Metabolomics and Spent Embryo Culture Medium
ClinicalTrials.gov study NCT06094439. IPD Sharing: NO. Countries: 1. Publications: 3.
Comparison of Individual Culture and Group Culture of Preimplantation Embryos in Human IVF Treatments
ClinicalTrials.gov study NCT01774006. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Comparative Study of Two Embryo Culture Systems
ClinicalTrials.gov study NCT02722252. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Influence of Post-thaw Embryo Culture Interval on Assisted Reproduction Success Rates
ClinicalTrials.gov study NCT03381001. IPD Sharing: NO. Countries: 1. Publications: 6.
The Effect of Embryo Culture Under Low Oxygen Tension on Pregnancy Rates
ClinicalTrials.gov study NCT01532193. IPD Sharing: Not stated. Countries: 1. Publications: 1.
ISM1 Versus EmbrioGen Media for Embryo Culture With Previous Failure Implantation in ART Treatments
ClinicalTrials.gov study NCT01689454. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Extended Culture of Day 3 to Day 5 Frozen Thawed Day 3 Embryos Versus Day 5 Frozen-Thawed Embryos
ClinicalTrials.gov study NCT05858333. IPD Sharing: Not stated. Countries: 1. Publications: 9.
Impact of Human Embryo Culture System on Live Birth
ClinicalTrials.gov study NCT01904006. IPD Sharing: NO. Countries: 1. Publications: 1.
Eeva™ System Study: Noninvasive Recording and Visualization of Individual Embryos Cultured to Blastocyst Stage
ClinicalTrials.gov study NCT01369446. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Optimizing the Temperature for Embryo Culture in In Vitro Fertilization
ClinicalTrials.gov study NCT01506089. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Outcomes of the Embryos of Different Ovarian Follicle Sizes, and the miRNA Detection in Their Blastocyst Culture
ClinicalTrials.gov study NCT05947526. IPD Sharing: NO. Countries: 1. Publications: 10.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.