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31 results for “Entamoeba histolytica”
Fig. 5 in Surface Coat Differences between and Non-Invasive Entamoeba dispar Invasive Entamoeba histolytica
Fig. 5. Confocal, and fluorescence intensity assays were carried-out using FITC-conjugated streptavidin (1: 100) for the localization of biotinylated proteins in E. dispar (A, C) and E. histolytica (B, D). Observation conditions were the same for both species, but had to be modified for E. dispar to improve image quality, since the high intensity of fluorescence produced distortion of the image. E – Graphical representation in arbitrary fluorescence units. Bar: 20 µm.
Fig. 1 in Surface Coat Differences between and Non-Invasive Entamoeba dispar Invasive Entamoeba histolytica
Fig. 1. Transmission electron photomicrographs of amoebas treated with ruthenium red and cationized ferritin. E. histolytica (A) and E. dispar (B) stained with ruthenium red. The stain on the cell surface of E. histolytica is seen as a dense solid layer. In contrast, in E. dispar the stain is observed as a slight deposit. Small groups of ferritin particles were observed in E. histolytica (C) while substantially large particles clumps were found in E. dispar (D). Bar: 0.1 µm.
Fig. 4 in Surface Coat Differences between and Non-Invasive Entamoeba dispar Invasive Entamoeba histolytica
Fig. 4. (A) Silver staining of the protein profile of E. dispar and E. histolytica, and densitometry analysis of the 60 kDa band as loading control. (B) Biotinylated patterns of membrane proteins of E. dispar and E. histolytica.
Fig. 2. A and B in Surface Coat Differences between and Non-Invasive Entamoeba dispar Invasive Entamoeba histolytica
Fig. 2. A and B. Cell coat of E. histolytica and E. dispar as observed in thin sections after treatment with Con A. The cell coat of E. histolytica (A) was observed as a thick layer of relatively homogenous electron- dense precipitate all along the cell surface. In contrast, in E. dispar, (B) the cell coat was strongly positive. Bar: 0.1 µm. C to D. Confocal and phase contrast microscopy images of amoebae after incubation with fluoresce- in-tagged Concanavalin A. As observed by confocal microscopy (C) and phase contrast (D) in E. histolytica trophozoites the displacement of surface lectin receptors formed a defined cap at the posterior pole of the cell, but such structure is not formed by E. dispar and only irregular patches were seen (E) confocal and (F) phase contrast. Bar: 20 µm.
Seroprevalence of Entamoeba histolytica at a voluntary counselling and testing centre in Tokyo: a cross-sectional study
<p><strong>Background</strong> Amebiasis, which is caused by <em>Entamoeba histolytica</em>, is a re-emerging public health issue owing to sexually transmitted infection (STI) in Japan. However, epidemiological data are quite limited.<br> <strong>Methods</strong> To reveal the relative prevalence of sexually transmitted <em>E. histolytica</em> infection to other STIs, we conducted a cross-sectional study at a voluntary counselling and testing (VCT) centre in Tokyo. Seroprevalence of <em>E. histolytica</em> was assessed according to positivity with an enzyme-linked immunosorbent assay for <em>E. histolytica</em>-specific IgG in serum samples collected from anonymous VCT clients.<br> <strong>Results</strong> Among 2,083 samples, seropositive rate for <em>E. histolytica</em> was 2.64%, which was higher than that for HIV-1 (0.34%, p < 0.001) and comparable to that for syphilis (rapid plasma reagin (RPR) 2.11%, p = 0.31). Positivity for <em>Chlamydia trachomatis</em> in urine by transcription-mediated amplification (TMA) was 4.59%. Seropositivity for <em>E. histolytica</em> was high among RPR- or Treponema pallidum hemagglutination (TPHA)-positive individuals and it was not different between clients with and without other STIs. Both seropositivity of <em>E. histolytica</em> and RPR were high among male clients. The seropositive rate for anti-<em>E. histolytica</em> antibody was positively correlated with age. TMA positivity for urine <em>C. trachomatis</em> was high among female clients and negatively correlated with age. Regression analysis identified that male sex, older age, and TPHA-positive results are independent risk factors of <em>E. histolytica</em> seropositivity.<br> <strong>Conclusions</strong> Seroprevalence of <em>E. histolytica</em> was 7.9 times higher than that of HIV-1 at a VCT centre in Tokyo, with a tendency to be higher among people at risk for syphilis infection. There is a need for education and specific interventions against this parasite, as a potentially re-<br> 65 emerging pathogen.</p>
Proteomic analysis of Entamoeba histolytica in vivo assembled pre-mRNA splicing complexes
<p>Dataset for publication "Proteomic analysis of <em>Entamoeba histolytica</em> in vivo assembled pre-mRNA splicing complexes"</p>
Seroprevalence of Entamoeba histolytica at a voluntary counselling and testing centre in Tokyo: a cross-sectional study
Open the record for dataset details and reuse information.
Data from: Queuine is a nutritional regulator of Entamoeba histolytica response to oxidative stress and a virulence attenuator
Open the record for dataset details and reuse information.
Transcriptional and functional analysis of trifluoromethionine resistance in Entamoeba histolytica
GEO Series GSE32314. Entamoeba histolytica. 33 samples. Type: Expression profiling by array.
Enteric bacteria increase the defenses against oxidative stress in Entamoeba histolytica
GEO Series GSE104434. Entamoeba histolytica. 33 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome profiling of Entamoeba histolytica in normal and stressed condition.
GEO Series GSE151975. Entamoeba histolytica. 6 samples. Type: Expression profiling by high throughput sequencing.
RNA-seq analysis of Entamoeba histolytica glycerol 3-phosphate dehydrogenase (G3PDH) and glycerol kinase (EhGK) gene silencing strain
GEO Series GSE240436. Entamoeba histolytica. 9 samples. Type: Expression profiling by high throughput sequencing.
Small RNA pyrosequencing in the protozoan parasite Entamoeba histolytica reveals strain-specific small RNAs that target virulence genes
GEO Series GSE43668. Entamoeba histolytica. 2 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Gene expression in phagocytic and non-phagocytic Entamoeba histolytica populations
GEO Series GSE41604. Entamoeba histolytica. 6 samples. Type: Expression profiling by array.
Effect of Rom1 silencing on gene expression in Entamoeba histolytica
GEO Series GSE21522. Entamoeba histolytica. 4 samples. Type: Expression profiling by array.
RNA-Seq expression analysis of Entamoeba histolytica trophozoites adapted to grow in the presence of toxic amount of the Nitric Oxide donor S-Nitrosoglutathione (GSNO)
GEO Series GSE77044. Entamoeba histolytica HM-1:IMSS. 5 samples. Type: Expression profiling by high throughput sequencing.
Impact of intestinal colonization and invasion on the Entamoeba histolytica transcriptome.
GEO Series GSE8484. Entamoeba histolytica. 7 samples. Type: Expression profiling by array.
Transcriptional profiling of Entamoeba histolytica trophozoites
GEO Series GSE4400. Entamoeba histolytica. 25 samples. Type: Expression profiling by array.
Characterization of an Entamoeba histolytica High-Mobility-Group Box Protein Induced during Intestinal Infection
GEO Series GSE12204. Entamoeba histolytica. 6 samples. Type: Expression profiling by array.
Effects of overexpression of EhMyb-dr on transcription in Entamoeba histolytica
GEO Series GSE13023. Entamoeba histolytica. 9 samples. Type: Expression profiling by array.
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