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11 results for “Epinephelus coioides”
Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish, Epinephelus coioides
<p>Figure S1. Cell localization of ly75, thy1, and dmc1 in adult testis of orange-spotted grouper. (A-C) Antisense probe signals of ly75, thy1, and dmc1. (D-F) Sense probe signals of ly75, thy1, and dmc1. Sg, Spermatogonium; Sc, Spermatocyte; St, spermatid. Scale Bars: 20 μm.</p> <p>Figure S2. Fluorescent immunostaining of antibodies in adult testis of orange-spotted grouper. (A-D and M-O) Fluorescence signals of Piwi, Dazl, Ssea1, Nanog, PCNA, Sycp3, and Dmc1. (E-H and P-R) Nuclei are counterstained with PI. (I-L and S-U) Merge images. Sg, Spermatogonium; Sc, Spermatocyte; St, spermatid; Sz, spermatozoa. Scale Bars: 20 μm.</p> <p>Figure S3. Derivation of a single colony from a single cell of GPT line. (A) A small colony after five days of culture. (B) A distinct colony after 10 days of culture. (C) A large colony containing hundreds of cells after 20 days of culture. Scale Bars: 20 μm in A; 50 μm in B; 200 μm in C.</p> <p>Figure S4. Prolonged cultivation of GPT cells under the lack of bFGF. (A-D) All GPT cells would differentiate into large epithelial-like cells and gradually die out during 25 days of culture under the lack of bFGF, LIF, and SCF (-/-). (E-H, J-M, and O-R) GPT cells consisted of some polygonal-like cells and many epithelial-like cells during 25 days of culture in the ESM media containing SCF and/or LIF. (I, N and S) All GPT cells transformed into very large epithelial-like cells and gradually died after 35 days of culture in the ESM media containing SCF and/or LIF. Scale Bars: 50 μm.</p> <p>Figure S5. Morphology of GPT cells under a condition of high cell confluence. (A) GPT cells were cultured for 14 days without subculture and (B) generated a few spherical cells (Arrows). Scale Bars: 50 μm in A; 20 μm in B.</p> <p>Figure S6. Establishment of a GPT cell line stably expressing green fluorescence protein. (A-C) Bright-field image, fluorescent image, and merged image of GPT cells after 48 hours of culture following electrotransfection with pEGFP-N3 plasmid. (D-F) Bright-field image, fluorescent image, and merged image of GPT cells after G418 resistance screening. Scale Bars: 200 μm in A-C; 100 μm in D-F.</p>
FIGURE 1. Prosorhynchus spp. from groupers. a, b Prosorhynchus tonkinensis n in Prosorhynchus Odhner, 1905 (Digenea: Bucephalidae) from the orange-spotted grouper Epinephelus coioides (Hamilton, 1822) (Epinephelidae), including Prosorhynchus tonkinensis n. sp., from the Gulf of Tonkin, Vietnam
FIGURE 1. Prosorhynchus spp. from groupers. a, b Prosorhynchus tonkinensis n. sp., note the spermatophore (a) and intended posterior extremity (b); c Prosorhynchus sp. A (VN); d Prosorhynchus sp. B (VN); e P. luzonicus; f Prosorhynchus maternus. Uterus in bold outline and treated as transparent. Scale bars: a, b, c, d=100 µm; e, f=200 µm.
FIGURE 5 in Prosorhynchus Odhner, 1905 (Digenea: Bucephalidae) from the orange-spotted grouper Epinephelus coioides (Hamilton, 1822) (Epinephelidae), including Prosorhynchus tonkinensis n. sp., from the Gulf of Tonkin, Vietnam
FIGURE 5. Visual comparison diagram for Prosorhynchus luzonicus from Vietnam.
Transcriptome analysis of the effect of Vibrio alginolyticus infection on the innate immunity-related complement pathway in Epinephelus coioides
GEO Series GSE63148. Epinephelus coioides. 3 samples. Type: Expression profiling by high throughput sequencing.
Integrative Analyses of mRNA and MicroRNA Expression Profiles Reveals the Innate Immune Mechanism for the Resistance to Vibrio parahaemolyticus Infection in Epinephelus coioides [miRNA-seq]
GEO Series GSE207125. Epinephelus coioides. 6 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Integrative Analyses of mRNA and MicroRNA Expression Profiles Reveals the Innate Immune Mechanism for the Resistance to Vibrio parahaemolyticus Infection in Epinephelus coioides [RNA-seq]
GEO Series GSE207124. Epinephelus coioides. 6 samples. Type: Expression profiling by high throughput sequencing.
Single-cell RNA sequencing reveals the gonadal dynamic changes of sex differentiation in hermaphroditic protogynous orange-spotted grouper, Epinephelus coioides
GEO Series GSE218835. Epinephelus coioides. 6 samples. Type: Expression profiling by high throughput sequencing.
Integrative Analyses of mRNA and MicroRNA Expression Profiles Reveals the Innate Immune Mechanism for the Resistance to Vibrio parahaemolyticus Infection in Epinephelus coioides
GEO Series GSE207127. Epinephelus coioides. 12 samples. Type: Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing.
Real-time quantitative PCR analysis of cortisol-indeced sex change in epinephelus coioides
GEO Series GSE142814. Epinephelus coioides. 16 samples. Type: Expression profiling by RT-PCR.
Expression data from infected skin and non-infected skin of Epinephelus coioides infected with Cryptocaryon irritans (C. irritans) and normal skin of E. coioides
GEO Series GSE97397. Epinephelus coioides. 9 samples. Type: Expression profiling by high throughput sequencing.
Identification of host microRNAs potentially related to SGIV infection in grouper Epinephelus coioides
GEO Series GSE64445. Epinephelus coioides. 1 samples. Type: Non-coding RNA profiling by high throughput sequencing.
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