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Fig. 19 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 19. Tenuibiotus zandrae sp. nov. Egg chorion morphology seen in SEM. A. Entire egg. B. Magnification of the egg surface. C–F. Details of the egg processes and surface between them. Filled flat arrowheads indicate thickenings/striae on the surface between processes and filled indented arrowheads indicate small tubercles on the process walls. Scale bars in μm.

Fig. 18 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 18. Tenuibiotus zandrae sp. nov. Egg chorion morphology seen in PCM. A. Midsection under 400× magnification. B. Surface under 400× magnification. C–D. Surface between processes under 1000× magnification. E–H. Midsections of processes of four different eggs under 1000× magnification. Filled flat arrowheads indicate thickenings/striae which are visible as dark dots and lines on the surface between processes. Scale bars in μm.

Fig. 14 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 14. Tenuibiotus zandrae sp. nov. Claws (paratypes). A–B. Claws II and IV seen in PCM, respectively. C–D. Claws I and IV seen in SEM, respectively. Filled indented arrowhead indicates horseshoe structure connecting the anterior and the posterior claw. Scale bars in μm.

Fig. 11 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 11. Tenuibiotus zandrae sp. nov. Body granulation seen in PCM (paratypes). A–B. Uniformly distributed granulation on the dorso-cephalic and dorso-caudal part of the body. C–D. Uniformly distributed granulation on the dorso-cephalic and dorso-caudal part of the body with small, random patches of lacking granulation. E–F. Uniformly distributed granulation on the ventral side of the body without and with small random patches lacking granulation, respectively. A–B, E and C–D, F are from two different paratypes. Scale bars in μm.

Fig. 8 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 8. Macrobiotus engbergi sp. nov. Egg chorion morphology seen in SEM. A. Entire egg. B. Magnification of the egg surface. C–D. Details of the terminal discs. Scale bars in μm.

Fig. 4 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 4. Macrobiotus engbergi sp. nov. Claws (paratypes). A–B. Claws III and IV seen in PCM, respectively. C–D. Claws III and IV seen in SEM, respectively. Filled flat arrowheads indicate double muscles attachments under the claws, empty flat arrowhead indicates inverted horseshoe structure under the external and the internal claw, whereas filled indented arrowhead indicates horseshoe structure connecting the anterior and the posterior claw. Scale bars in μm.

Fig. 5 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 5. Macrobiotus engbergi sp. nov. Buccal apparatus and the oral cavity armature seen in PCM (holotype, IZiBB, slide GL.052.22). A. Dorso-ventral projection of the entire buccal apparatus. B–C. Oral cavity armature visible in dorsal (B) and ventral (C) view, respectively. D–E. Placoid morphology visible in dorsal (D) and ventral (E) view, respectively. Filled flat arrowheads indicate the second band of teeth in the oral cavity, empty flat arrowheads indicate the third band of teeth in the oral cavity, empty indented arrowheads indicate central constrictions in the first macroplacoids and subterminal constriction in the second macroplacoids. Scale bars in μm.

Fig. 12 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 12. Tenuibiotus zandrae sp. nov. Patches of dense granulation on legs seen in PCM (paratypes). A. External granulation on leg III (patch of dense granulation encircled). B. Internal granulation on leg III. C. Granulation on leg IV. Scale bars in μm.

Fig. 21 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 21. Tenuibiotus voronkovi (Tumanov, 2007) Egg chorion morphology seen in PCM. A. Midsection under 400× magnification. B. Surface between processes under 1000× magnification. C–J. Details of egg processes under 1000× magnification. Filled flat arrowhead indicates thickenings/striae/sculpture which are visible as dark dots on the surface between processes and indented empty arrowheads indicate broken apices of the egg processes. Scale bars in μm.

Fig. 20 in Integrative taxonomy identifies two new tardigrade species (Eutardigrada: Macrobiotidae) from Greenland

Fig. 20. Tenuibiotus voronkovi (Tumanov, 2007). Body granulation seen in PCM. A. Uniformly distributed granulation of uniform size on the dorso-medial part of the body (cephalic region, paratype). B. Patch of dorso-lateral granulation composed of granules of different size (holotype). Scale bars in μm.

FIG. 13 in Revisiting Calohypsibiidae and Microhypsibiidae: Fractonotus Pilato, 1998 and its phylogenetic position within Isohypsibiidae (Eutardigrada: Parachela)

FIG. 13. — The phylogenetic position of Fractonotus verrucosus (Richters, 1900) n. comb. on the Bayesian Inference and Maximum Likelihood cladogram constructed from currently available 18S rRNA sequences for Isohypsibioidea and Hypsibioidea, with a Milnesium spp. sequence as the outgroup (see also Table 2). Values above branches indicate posterior probability values (BI), whereas values under branches show bootstrap values (ML). Branches with support below 0.9 in BI (70% in ML) were collapsed.

FIG. 10 in Revisiting Calohypsibiidae and Microhypsibiidae: Fractonotus Pilato, 1998 and its phylogenetic position within Isohypsibiidae (Eutardigrada: Parachela)

FIG. 10. — Schematic comparison of stylet furcae in: A, Fractonotus Pilato, 1998; B, Calohypsibius Thulin, 1928; C, Isohypsibius Thulin, 1928.

FIG. 4 in Revisiting Calohypsibiidae and Microhypsibiidae: Fractonotus Pilato, 1998 and its phylogenetic position within Isohypsibiidae (Eutardigrada: Parachela)

FIG. 4. — Genus Calohypsibius Thulin, 1928 s.s., after exclusion of F. verrucosus n. comb. (PCM): A, Calohypsibius ornatus (Richters, 1900); B, Calohypsibius maliki Michalczyk & Kaczmarek, 2005; C, Calohypsibius schusteri Nelson & McGlothlin, 1996. Scale bars: 10 μm.

FIG. 8 in Revisiting Calohypsibiidae and Microhypsibiidae: Fractonotus Pilato, 1998 and its phylogenetic position within Isohypsibiidae (Eutardigrada: Parachela)

FIG. 8. — Buccal apparatus of Fractonotus verrucosus (Richters, 1900) n. comb. (SEM): A, oral cavity armature, the arrowhead indicates the row of conical teeth; B, the buccal crown and both dorsal and ventral apophyses for insertion of stylet muscles (AISM) in lateral view; C, buccal tube, empty arrowheads indicate lateral thickenings; D, stylet furca; E, pharynx in dorsal view; F, pharynx in lateral view. Scale bars: A, D, 0.5 μm; B, 1 μm; C, E, F, 2 μm.

FIG. 7. — A-E in Revisiting Calohypsibiidae and Microhypsibiidae: Fractonotus Pilato, 1998 and its phylogenetic position within Isohypsibiidae (Eutardigrada: Parachela)

FIG. 7. — A-E, Fractonotus spp., F-G, Calohypsibius spp. and H-I, Isohypsibius spp., elliptical organs and details of the buccal apparatus (PCM): Fractonotus verrucosus (Richters, 1900) n. comb.: A, cephalic region, empty arrowheads indicate elliptical organs; B, dorsal view, incised arrowhead indicates buccal tube thickening, and incised empty arrowhead points to the furca; C, ventral view; Fractonotus gilvus (Biserov, 1986) n. comb.; D, ventral view, incised empty arrowhead points to the furca; Fractonotus verrucosus (Richters, 1900) n. comb.; E, lateral view, arrowhead indicates the posterior portion of dorsal apophysis in the shape of stumpy hook; F, Calohypsibius ornatus (Richters, 1900), lateral view, arrowhead indicates the anterior portion of dorsal apophysis in the shape of stumpy hook; G, Calohypsibius schusteri Nelson & McGlothlin, 1996, arrowhead indicates the anterior portion of dorsal apophysis in the shape of stumpy hook, and arrow points to the visible peribuccal papulae; H, Isohypsibius prosostomus (Thulin, 1928); I, Isohypsibius coulsoni Kaczmarek, Zawierucha, Smykla, Michalczyk, 2012. Scale bars: 10 μm.

Fig. 11 in Integrative description of Macrobiotus canaricus sp. nov. with notes on M. recens (Eutardigrada: Macrobiotidae)

Fig. 11. Macrobiotus cf. recens Cuénot, 1932, Gran Canaria, oral cavity armature seen under SEM from different angles. A. View of the dorsal portion. B. View of the ventral portion. Filled arrowheads indicate the teeth of the first band, empty arrowheads indicate the teeth of the second band, median teeth are marked with M, and lateral teeth are marked with L. Scale bars in μm.

Fig. 7 in Integrative description of Macrobiotus canaricus sp. nov. with notes on M. recens (Eutardigrada: Macrobiotidae)

Fig. 7. Macrobiotus canaricus sp. nov., egg chorion morphology seen under SEM. A. Entire egg. B. Close up of the egg surface and processes. C–D. Details of the egg surface. E–F. Details of the terminal discs. Arrowheads indicate granules in the centre of the terminal discs. Scale bars in μm.

Fig. 5 in Integrative description of Macrobiotus canaricus sp. nov. with notes on M. recens (Eutardigrada: Macrobiotidae)

Fig. 5. Macrobiotus canaricus sp. nov., paratypes, claws. A–B. Claws III and IV seen under PCM. C–D. Claws I and IV seen under SEM. Arrowheads indicate faint muscle attachments under claws; Figs A and B assembled from several photos. Scale bars in μm.

Fig. 3 in Integrative description of Macrobiotus canaricus sp. nov. with notes on M. recens (Eutardigrada: Macrobiotidae)

Fig. 3. Macrobiotus canaricus sp. nov., paratypes, cuticular structures on legs. A–B. The patch of granulation on the external surface of leg III with a large oval cuticular pore at the centre of the patch (PCM and SEM, respectively). C–D. The patch of granulation and the cuticular bulge (pulvinus) on the internal surface of leg II (PCM and SEM, respectively). E–F. Granulation on leg IV (PCM and SEM, respectively). Filled flat arrowheads indicate the large pore in the centre of the external granulation patch, filled indented arrowheads indicate the cuticular fold (pulvinus) on the internal leg surface, and empty indented arrowheads indicate granulation on the internal leg surface. Scale bars in μm.

Fig. 1 in Integrative description of Macrobiotus canaricus sp. nov. with notes on M. recens (Eutardigrada: Macrobiotidae)

Fig. 1. Macrobiotus canaricus sp. nov., holotype, habitus. A. Dorso-ventral projection (Hoyer's medium, PCM). B. Dorsal view under SEM. Scale bars in μm.