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3 results for “FIB tomography”
FIB-SEM tomography datasets of entire single-cell human monocyte derived macrophages
<p>FIB-SEM tomography was used to determine the absolute number of internalized gold nanoparticles in GM-CSF and M-CSF human monocyte-derived macrophages at the single-cell level. All experiments were performed using a Thermo Scientific Scios 2 Dual Beam microscope (Thermo Fisher Scientific, Waltham, MA, USA). A selected cell was protected with a platinum (Pt) layer of 1 µm thickness (30 kV and current of 1 nA). Then, a trench of 5 µm in depth was milled on the front face and each side of the cell of interest using the ion beam at 7 nA current. Finally, the front face of the volume of interest was polished with an ion beam current of 1 nA until the beginning of the cell was visible to optimize the focus and contrast. FIB-SEM tomography of the whole cell was performed using the FEI Slice and View software (Thermo Fisher Scientific, Waltham, MA, USA, version 4.1). The electron beam acceleration voltage was set to 5 kV, the current to 0.4 nA, the resolution to 1536 × 1024 pixels, and the dwell time to 30 µs. Images were acquired in immersion mode with the backscattered electron detector, yielding clear signals from AuNPs due to the detector's sensitivity to backscattered electrons, which correlates with the atomic number of Au (Z = 79). The ion beam operating with a current of 1 nA current at 30 kV was used to slice through the cell of interest at an interval of 18 nm and a depth of 5 µm. The datasets available on the repository have been aligned and cropped.</p>
FIB-tomography data of Ni-YSZ anodes for Solid Oxide Fuel Cells (SOFC): Comparison of pristine and degraded materials (before/after redox cycling)
<p><em>Contents: </em></p> <p>This dataset contains 3D image stacks acquired with FIB-tomography from Ni-YSZ cermet anodes for Solid Oxide Fuel Cells (SOFC).</p> <p>The data was collected from three different Ni-YSZ anodes (fine-, medium- and coarse-grained). Each of these anodes was investigated first in pristine state (after sintering and reduction) and then also in degraded state (after exposure to 8 redox cycles).</p> <p>The 6 tomographs are then presented as stacks of 2D-tiff-images in 2 different versions: as gray-scale images (raw data) and as segmented images (Ni=white, YSZ=gray and pores=black). In total this gives 12 image stacks.</p> <p><strong>Further details</strong>, such as the voxel resolutions and image window sizes are listed in the downloadable excel file (<strong>2_3D_Data_Info.xlsx</strong>).</p> <p> </p> <p><em>Scientific Context: </em></p> <p>The microstructures of the cermet anodes were investigated for the purpose of optimizing the anode performance, which depends on effective transport properties (i.e. conductivity of ions in YSZ and of electrons in Ni, as well as diffusivity of fuel/gas in the pores). Furthermore the anode performance also depends on the catalytic/electrochemical activity (i.e. Ni-surface area and three phase boundary length TPBL). The microstructure characteristics have a strong influence on effective properties, electrochemical activity and associated anode performance. Furthermore, microstructure degradation (e.g. by Ni-coarsening) may lead to performance loss over time.</p> <p>Hence, the investigations focus on a fundamental, quentitative understanding of the relationships between microstructure characteristics and effective properties. The study reveals quantitative descriptions of all relevant microstructure characteristics (porosity, tortuosity, constrictivity, surface/interface areas, TPBL) and of the corresponding effective transport porperties (electric and ionic.conductivities). The corresponding anode performance was characterized by impedance spectroscopy.</p> <p>The quantitative <strong>results of the microstructure investigation were published</strong> in:</p> <p><strong>Pecho et al</strong> 2015a (doi:10.3390/ma8095265),</p> <p><strong>Pecho et al</strong> 2015b (doi:10.3390/ma8105370),</p> <p><strong>Holzer et al</strong> 2013 (doi: 10.1016/j.jpowsour.2013.05.047),</p> <p><strong>Holzer et al</strong> 2011a (doi: 10.1016/j.jpowsour.2010.08.017) and</p> <p><strong>Holzer et al</strong> 2011b (doi: 10.1016/j.jpowsour.2010.08.006).</p>
FIB-tomography data of porous Zr-oxide fabricated with varying sintering conditions
<p>This dataset contains 3D data acquired with FIB-tomography from porous Zr-oxide. The pore structures of the ceramic materials were investigated in context with optimization of transport properties (aimed for low viscous flow/permeability and high electric conduction via liquid electrolyte in the pores), so that the Zr-oxide is suitable for application as liquid junction (diaphragm) in pH-sensors. Further details on the scientific background are given in Holzer et al., 2016, Materials & Design, 99, 314–327. (http://doi.org/10.1016/j.matdes.2016.03.034).</p> <p> </p> <p>The tomographs represent 9 ceramic materials that were produced with different sintering temperatures (1250, 1300, 1325 and 1350°C) and different sintering times (1, 2 and 3 hours). The study includes quantitative descriptions of relevant microstructure characteristics (porosity, tortuosity, constrictivity, hydraulic radius) and corresponding effective transport prorperties (permeability, conductivity).</p> <p> </p> <p>Each tomograph is presented as stack of 2D-tiff-images in two different versions: as gray-scale images (raw data) and as binarized images (segmented, with solid=black and pores=white). The voxel resolution is 10x10x10 nm for all 18 image stacks.</p> <p> </p> <p>More details on the image data (e.g. voxel nr, image window size, data size) are given in the downloadable file '2_3D_Data_Info.xlsx'.</p>
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OpenNeuro
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