Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
2
datasets available to search
ShareScore release 0.9.0
Dataset results
2 results for “GCaMP-X”
Chronic Ca2+ imaging of cortical neurons with long-term expression of GCaMP-X
<p><span>Dynamic Ca<sup>2+</sup> signals reflect acute changes in membrane excitability and also mediate signaling cascades in chronic processes. In both cases, chronic </span><span>Ca<sup>2+</sup></span><span> imaging is often desired but challenged by the cytotoxicity intrinsic to calmodulin (CaM)-based GCaMP, a series of genetically-encoded </span><span>Ca<sup>2+</sup></span><span> indicators that have been widely applied. Here, we demonstrate the performance of GCaMP-X in chronic </span><span>Ca<sup>2+</sup></span><span> imaging of cortical neurons, where GCaMP-X by design is to eliminate the unwanted interactions between the conventional GCaMP and endogenous (apo)CaM-binding proteins. By expressing in adult mice at high levels over an extended time frame, GCaMP-X showed less damage and improved performance in two-photon imaging of sensory (whisker-deflection) responses or spontaneous </span><span>Ca<sup>2+</sup></span><span> fluctuations, in comparison with GCaMP. Chronic </span><span>Ca<sup>2+</sup></span><span> imaging of one month or longer was conducted for cultured cortical neurons expressing GCaMP-X, unveiling that spontaneous/local </span><span>Ca<sup>2+</sup></span><span> transients progressively developed into autonomous/global </span><span>Ca<sup>2+</sup></span><span> oscillations. Along with the morphological indices of neurite length and soma size, the major metrics of oscillatory </span><span>Ca<sup>2+</sup></span><span>, including rate, amplitude and synchrony were also examined. Dysregulations of both neuritogenesis and </span><span>Ca<sup>2+</sup></span><span> oscillations became discernible around 2</span><span>–</span><span>3 weeks after virus injection or drug induction to express GCaMP in newborn or mature neurons, which were exacerbated by stronger or prolonged expression of GCaMP. In contrast, neurons expressing GCaMP-X were significantly less damaged or perturbed, altogether highlighting the unique importance of oscillatory </span><span>Ca<sup>2+</sup></span><span> to neural development and neuronal health. In summary, GCaMP-X provides a viable solution for </span><span>Ca<sup>2+</sup></span><span> imaging applications involving long-time and/or high-level expression of </span><span>Ca<sup>2+</sup></span><span> probes. </span></p>
Chronic Ca2+ imaging of cortical neurons with long-term expression of GCaMP-X
Open the record for dataset details and reuse information.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.