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248 results for “Gene editing”
Illumina sequencing data of Agro-mediated gene-edited apple lines
<p>FastaQ pair-end Illumina sequencing data of the Dipm1/4, Hipm1, and Mlo19 genes from the different apple Agro-edited lines obtained in the project. GA = Gala; GD = Golden Delicious</p> <p>Lines included are:</p> <p>GA1, GA3, GA4, GA5, and GA WT</p> <p>GD1, 2, 6, 10, 11, 12, 15, 17, 18, 19, 20, 21, 23, 24, 26, 27, 29, 31, 33, 36, 37, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, and WT</p>
Public views on gene editing and its uses
<p>Rapid advances in genome editing, including CRISPR-Cas9 endonucleases, and their potential application in medicine and enhancement have been hotly debated by scientists and ethicists. Although a veto on germ line gene editing has been proposed<sup>1</sup>, the use of gene editing on human cells in the clinical context remains controversial, particularly for interventions aimed at enhancement<sup>2</sup>. In a report on human genome editing the US National Academy of Sciences (NAS) note that “important questions raised with respect to genome editing include how to incorporate societal values into salient clinical and policy consideration”<sup>3</sup>. We report here our research that opens a window onto what the public think.</p>
Genome editing of LKB1 gene by CRISPR/Cas9 in lung cancer cells and evaluation of its role in metformin and cisplatin response.
<pre>LKB1 is an important upstream inhibitor of the mTOR/S6Ks pathway. Loss of LKB1 is often associated with cancer, including in A549 lung cancer cell line, boosting the transformation of pre-malignant neoplasic cells. Metformin, a natural compound derived from Galega officinalis, is mainly used as a treatment for diabetes mellitus type 2 (DM2), but recently, it has been associated to lower incidence of cancer. One of the main mechanisms is by activation of AMPK through different pathways, including LKB1 activation. Activation of AMPK inhibits the mTOR pathway, protein synthesis and thus cell growth. The combination of metformin and cisplatin, a main chemothepeutic drug for lung cancer, has shown to improve treatment of cancer cells to cisplatin and also to hinder the cisplatin associated resistance common in lung cancer. Here we aim to use the CRISPR/Cas9 technology to correct the mutation presented in A549 lung cancer cells and improve their response to metformin or to the combination between metformin and cisplatin. Besides, we will assess the mTOR signaling pathway status, as well as cell growth, proliferation, cell cycle and sensitivity to apoptosis induction by cisplatin. This project may be useful as a proof of principle that in the future therapies must consider the genomic background of cancer cells before administration of a specific anticancer drug.<br><br></pre>
A photo-switchable gold nanoformulation based on the dCas9 protein for spatiotemporal controlled gene editing activation in vivo
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A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei - sequencing data
<p>This holds raw sequencing data, and extracted sgRNA counts </p>
Genome report: Genome sequence of 1S1, a transformable and highly regenerable diploid potato for use as a model for gene editing and genetic engineering
<p>Generation of a genomic resource for a readily transformable diploid potato would provide a resource for high throughput functional analysis in potato. The heterozygous <em>Solanum tuberosum</em> Group Phureja clone 1S1 has a high regeneration rate, self-fertility, desirable tuber traits and is amenable to <em>Agrobacterium</em>-mediated transformation. To create a contiguous genome assembly, a homozygous doubled monoploid of 1S1 (DM1S1) was sequenced using 44 Gbp of long reads generated from Oxford Nanopore Technologies (ONT), yielding a 736 Mb assembly that encoded 31,145 protein-coding genes. The final assembly for DM1S1 represents a nearly complete genic space, shown by the presence of 99.6% (C:99.5%[S:97.8%, D:1.7%],F:0.1%,M:0.4%,n:1614) of the Benchmarking Universal Single Copy Orthologs. Variant analysis with Illumina reads from 1S1 was used to deduce its alternate haplotype using the variant calling tools Strelka2 (v2.9.10), GATK's Haplotypecaller (v4.1.4.1), and Freebayes (v1.3.2). These variants were used to create consensus fasta sequences with the DM1S1 assembly using bcftools (v1.9.64).</p>
Data for: An AAV-CRISPR/Cas9-strategy for gene editing across divergent rodent species: Targeting neural oxytocin receptors as a proof of concept
<p>A major issue in neuroscience is the poor translatability of research results from preclinical studies in animals to clinical outcomes. Comparative neuroscience can overcome this barrier by studying multiple species to differentiate between species-specific and general mechanisms of neural circuit functioning. Targeted manipulation of neural circuits often depends on genetic dissection, and use of this technique has been restricted to only a few model species, limiting its application in comparative research. However, ongoing advances in genomics make genetic dissection attainable in a growing number of species. To demonstrate the potential of comparative gene editing approaches, we developed a viral-mediated CRISPR/Cas9 strategy that is predicted to target the oxytocin receptor (<em>Oxtr</em>) gene in >80 rodent species. This strategy specifically reduced OXTR levels in all evaluated species (n=6), without causing gross neuronal toxicity. Thus, we show that CRISPR/Cas9-based tools can function in multiple species simultaneously. Thereby, we hope to encourage comparative gene editing and improve the translatability of neuroscientific research.</p>
Genome report: Genome sequence of 1S1, a transformable and highly regenerable diploid potato for use as a model for gene editing and genetic engineering
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Data for: An AAV-CRISPR/Cas9-strategy for gene editing across divergent rodent species: Targeting neural oxytocin receptors as a proof of concept
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StArt Protocol for systematic review: Use of CRISPR technology in gene editing for tolerance to biotic factors in plants: A systematic review.
<p>StArt Protocol for systematic review: Use of CRISPR technology in gene editing for tolerance to biotic factors in plants: A systematic review.</p>
Data from: Consequences of gene editing of PRLR on thermotolerance, growth, and male reproduction in cattle
<p>Global warming is a major challenge to the sustainable and humane production of food because of the increased risk of livestock to heat stress. Here, the example of the prolactin receptor (<em>PRLR</em>) gene is used to demonstrate how gene editing can increase the resistance of cattle to heat stress by the introduction of mutations conferring thermotolerance. Several cattle populations in South and Central America possess natural mutations in <em>PRLR</em> that result in affected animals having short hair and being thermotolerant. CRISPR/Cas9 technology was used to introduce variants of <em>PRLR</em> in two thermosensitive breeds of cattle – Angus and Jersey. Gene-edited animals exhibited superior ability to regulate vaginal temperature (heifers) and rectal temperature (bulls) compared to animals that were not gene-edited. Moreover, gene-edited animals exhibited superior growth characteristics. There was no evidence for deleterious effects of the mutation on carcass characteristics or male reproductive function. These results indicate the potential for reducing heat stress in relevant environments to enhance cattle productivity. <strong><br></strong></p>
Development of a homeolog-specific gene editing system in an evolutionary model for the study of polyploidy in nature
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Data from: Consequences of gene editing of PRLR on thermotolerance, growth, and male reproduction in cattle
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Data from: Orthogonal transcriptional modulation and gene editing using multiple CRISPR/Cas systems
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Data from: Chromosome-level reference genome assembly and gene editing of the dead-leaf butterfly Kallima inachus
<p class="p">The leaf resemblance of <i><span>Kallima</span></i> (Nymphalidae) butterflies<i> </i>is an important ecological adaptive mechanism that increases survival. However, the genetic mechanism underlying ecological adaptation remains unclear owing to a dearth of genomic information. Herein, we revealed the karyotype (n = 31) of the dead-leaf butterfly <i><span>Kallima inach</span></i><i><span>us</span></i>, assembled its high-quality chromosome-level reference genome (568.92 Mb; contig N50: 19.20 Mb), and identified its Z and candidate W chromosomes. To our knowledge, this is the first study to report on these aspects of this species. In the assembled genome, 15,309 protein-coding genes and 49.86% repeat elements were annotated. Phylogenetic analysis showed that <i><span>K. inachus</span></i> diverged from <i><span>Melitaea cinxia </span></i>(no leaf resemblance), both of which are in Nymphalinae, around 40 million years ago. Demographic analysis indicated that the effective population size of <i><span>K. inach</span></i><i><span>us</span></i> decreased during the last interglacial period in the Pleistocene. The wings of adults with the pigmentary gene <i><span>ebony</span></i> knocked out using CRISPR/Cas9 showed phenotypes in which the orange dorsal region and entire ventral surface darkened, suggesting its vital role in the ecological adaption of dead-leaf butterflies. Our results provide important genome resources for investigating the genetic mechanism underlying protective resemblance in dead-leaf butterflies and insights into the molecular basis of protective coloration.</p>
PRISMA Checklist for for systematic review: Use of CRISPR technology in gene editing for tolerance to biotic factors in plants: A systematic review.
<p>PRISMA Checklist for for systematic review: Use of CRISPR technology in gene editing for tolerance to biotic factors in plants: A systematic review.</p>
PRISMA Checklist for systematic review: Use of CRISPR technology in gene editing for tolerance to biotic factors in plants: A systematic review.
<p>PRISMA Checklist for systematic review: Use of CRISPR technology in gene editing for tolerance to biotic factors in plants: A systematic review.</p>
PRISMA Checklist for systematic review: Use of CRISPR technology in gene editing for tolerance to biotic factors in plants: A systematic review.
<p>PRISMA Checklist for systematic review: Use of CRISPR technology in gene editing for tolerance to biotic factors in plants: A systematic review.</p>
StArt Protocol for systematic review: Use of CRISPR technology in gene editing for tolerance to biotic factors in plants: A systematic review.
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A Transfection-Free Approach of Gene Editing via a gold-based nanoformulation of the Cas9 protein
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.