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294 results for “Granulosa cells”
Granulosa cell transcriptome data from four different time points before and up to 48 hours after PMSG induction to GTH-depend phase in mice
<p>GTH-dependent follicle development begins with small antrum follicles and ends with preovulatory follicles. GTH-dependent follicle development is mainly controlled by gonadotropins, and the development time is 48h. The purpose of this study is to monitor the changes in gene expression of granulosa cells at four different time points during the GTH-dependent phase to increase our understanding of human GTH-dependent follicle development.Granulosa cell mRNA profiles of GTH-depend phase in mice.There are 12 samples represented four time points 0h (n=3), 12h (n=3), 24h (n=3) and 48h (n=3)</p>
Expression and function of Twitch-2B in the granulosa cells of mouse ovarian follicles
<p>Briefly, antral follicles were dissected from 23- to 26-d-old <em>RR8; Bact_Cre mice</em>. Follicles were cultured for 24–30 h on organotypic membranes (Millipore; cat. No. PICMORG50) in the presence of follicle-stimulating hormone. The follicle was held in a perfusion slide consisting of a plastic slide (ibidi) and a glass coverslip and assembled using silicon grease. The slide was constructed such that medium containing ovine LH (National Hormone and Peptide Program; 10 μg/mL) could be perfused through a 200-μm-deep channel holding the follicle. Temperature was maintained at 30–34 °C, by use of a warm air blower (Nevtek). Preovulatory Follicles were imaged using a Zeiss Pascal confocal microscope with a 40X/1.2 NA objective. Images were collected every 10 seconds. Measurements were corrected for autofluorescence and for spectral bleed-through of CFP into the YFP channel. Ratios were calculated by dividing the mean CFP intensity in each region of interest by the mean YFP intensity. Data analysis was done using ImageJ and Excel software. Data is representative of 4 follicles.</p>
Lipidomics of exosomes derived from granulosa cells
<p>The mapped data files generated from the analysis of the pathway in MetExploreViz.<br><span>Lipid content categorized by saturation level.</span></p>
Identification of ovarian granulosa cells and effect examination
<p>Figure S1. Identification of ovarian granulosa cells.<br> Figure S2. The mensuration of over-expression effect. (A: The relative mRNA level of over-expression MT1, MT2, Cry1, and Cry2 at 24h by Q-PCR; B: Western blotting (WB) results of MT1, MT2, CRY1, and CRY2 protein levels, respectively. Data are presented as the mean ± standard error of the mean ( SEM) of triplicate experiments. ** P < 0.01, * P < 0.05)<br> Figure S3. The mensuration of silencing effect. (A-D: Q-PCR assay of the MT1, MT2, Cry1, and Cry2 mRNA levels in ovarian granular cells after transfection of siRNAs against MT1, MT2, Cry1, and Cry2, respectively. E: Western blotting (WB) results of MT1, MT2, CRY1, and CRY2 protein levels in ovarian granular cells after transfection of siRNAs against MT1, MT2, Cry1, and Cry2, respectively. Data are presented as the mean ± standard error of the mean ( SEM) of triplicate experiments. ** P < 0.01, * P < 0.05)</p>
Polybrominated diphenyl ethers in human follicular fluid dysregulate mural and cumulus granulosa cell gene expression
<p>Polybrominated diphenyl ethers (PBDEs), a major class of flame retardants incorporated into numerous consumer products, leach out into dust resulting in widespread exposure. There is evidence from <i>in vitro</i> and <i>in vivo</i> animal studies that PBDEs affect ovarian granulosa cell function and follicular development, yet human studies of their association with female infertility are inconclusive. Here, we tested the hypothesis that exposure to the PBDEs in follicular fluid is associated with dysregulation of gene expression in the mural and cumulus granulosa cells collected from women undergoing in vitro fertilization by intracytoplasmic sperm injection. The median concentration of the ∑<sub>10</sub>PBDEs detected in the follicular fluid samples (n=37) was 15.04 pg/g wet weight. RNA microarray analyses revealed that many genes were differentially expressed in mural and cumulus granulosa cells. Highest <i>vs</i>. lowest quartile exposure to the Σ<sub>10</sub>PBDEs or to two predominant PBDE congeners, BDE-47 or BDE-153, was associated with significant effects on gene expression in both cell types. Mural granulosa cells were generally more sensitive to PBDE exposure compared to cumulus cells. Overall, gene expression changes associated with BDE-47 exposure were similar to those for ∑<sub>10</sub>PBDEs but distinct from those associated with BDE-153 exposure. Interestingly, exposure to BDE-47 and ∑<sub>10</sub>PBDEs activated the expression of genes in pathways that are important in innate immunity and inflammation. To the best of our knowledge, this is the first demonstration that exposure to these environmental chemicals is associated with the dysregulation of pathways that play an essential role in ovulation.</p>
Ketoconazole as Inhibitor of the Enzyme CYP17 in Locally Advanced or Disseminated Granulosa Cell Tumour of Ovary
ClinicalTrials.gov study NCT01584297. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Impact of FSH Dosage During Ovarian Stimulation for IVF/ICSI in Granulosa Cells
ClinicalTrials.gov study NCT05330130. IPD Sharing: NO. Countries: 1. Publications: 19.
Enzalutamide in Metastatic or Advanced Non-resectable Granulosa Cell Ovarian Tumors: GREKO III Study
ClinicalTrials.gov study NCT03464201. IPD Sharing: NO. Countries: 1. Publications: 1.
Orteronel (TAK-700) in Metastatic or Advanced Non-resectable Granulosa Cell Ovarian Tumors. The Greko II Study.
ClinicalTrials.gov study NCT02101684. IPD Sharing: Not stated. Countries: 1. Publications: 1.
AMHR2 Polymorphism in Blood and Granulosa Cells in Unexplained Infertility
ClinicalTrials.gov study NCT05592730. IPD Sharing: NO. Countries: 1. Publications: 3.
Tachykinin and Kisspeptin Expression in Human Granulosa and Cumulus Cells
ClinicalTrials.gov study NCT02877992. IPD Sharing: Not stated. Countries: 1. Publications: 2.
The Comparison of Granulosa Cell Apoptosis Rates With or Without Luteinizing Hormone Administration in Poor Responders.
ClinicalTrials.gov study NCT03527823. IPD Sharing: UNDECIDED. Countries: 1. Publications: 9.
Effect of Increased Circulating Androgens on Granulosa Cell Responses to FSH.
ClinicalTrials.gov study NCT02389088. IPD Sharing: NO. Countries: 0. Publications: 1.
Effect of Fentanyl on Main Opioid Receptor (OPRM1) on Human Granulosa Cells.
ClinicalTrials.gov study NCT03248076. IPD Sharing: UNDECIDED. Countries: 1. Publications: 3.
Study to Test the Drug Darolutamide Along With the Drugs Leuprolide Acetate and Exemestane in Patients With Recurrent Ovarian Granulosa Cell Tumors
ClinicalTrials.gov study NCT06169124. IPD Sharing: YES. Countries: 1. Publications: 0.
SIRT3 Expression in Granulosa Cells and the Levels of Follicular Fluid Metabolites Among POR Subgroups
ClinicalTrials.gov study NCT06617988. IPD Sharing: NO. Countries: 1. Publications: 9.
Intrauterine Flushing With Follicular Fluid Plus Granulosa Cells
ClinicalTrials.gov study NCT04077970. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Role of DNA Damage of Granulosa Cell on Oocyte Quality and in Vitro Fertilization Outcome
ClinicalTrials.gov study NCT03345030. IPD Sharing: NO. Countries: 1. Publications: 3.
Polybrominated diphenyl ethers in human follicular fluid dysregulate mural and cumulus granulosa cell gene expression
Open the record for dataset details and reuse information.
To Explore the Effect of GH on Outcomes of IVF and Telomerase Activity of Granulosa Cells in Women With POR
ClinicalTrials.gov study NCT04384744. IPD Sharing: NO. Countries: 0. Publications: 1.
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