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22 results for “H295R”
Data from: High-throughput H295R steroidogenesis assay: utility as an alternative and a statistical approach to characterize effects on steroidogenesis
The U.S. Environmental Protection Agency Endocrine Disruptor Screening Program and the Organization for Economic Co-operation and Development (OECD) have used the human adrenocarcinoma (H295R) cell-based assay to predict chemical perturbation of androgen and estrogen production. Recently, a high-throughput H295R (HT-H295R) assay was developed as part of the ToxCast program that includes measurement of 11 hormones, including progestagens, glucocorticoids, androgens, and estrogens. To date, 2012 chemicals have been screened at one concentration; of these, 656 chemicals have been screened in concentration-response. The objectives of this work were to: 1) develop an integrated analysis of chemical-mediated effects on steroidogenesis in the HT-H295R assay; and, 2) evaluate whether the HT-H295R assay predicts estrogen and androgen production specifically via comparison with the OECD-validated H295R assay. To support application of HT-H295R assay data to weight-of-evidence and prioritization tasks, a single numeric value based on Mahalanobis distances was computed for 654 chemicals to indicate the magnitude of effects on the synthesis of 11 hormones. The maximum mean Mahalanobis distance (maxmMd) values were high for strong modulators (prochloraz, mifepristone) and lower for moderate modulators (atrazine, molinate). Twenty-five of 28 reference chemicals used for OECD validation were screened in the HT-H295R assay, and produced qualitatively similar results, with accuracies of 0.90/0.75 and 0.81/0.91 for increased/decreased testosterone and estradiol production, respectively. The HT-H295R assay provides robust information regarding estrogen and androgen production, as well as additional hormones. The maxmMd from this integrated analysis may provide a data-driven approach to prioritizing lists of chemicals for putative effects on steroidogenesis.
Data from: High-throughput H295R steroidogenesis assay: utility as an alternative and a statistical approach to characterize effects on steroidogenesis
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Gene expression profiles of human adrenocortical NCI-H295R cells treated with cortisol biosynthesis inhibitors
GEO Series GSE236435. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Gene expression signatures in cell cycle sorted cells [H295R_mRNA_array]
GEO Series GSE73256. Homo sapiens. 8 samples. Type: Expression profiling by array.
The role of NFATc4 on aldosterone production in NCI-H295R human adrenocortical cell line
GEO Series GSE225735. Homo sapiens. 15 samples. Type: Expression profiling by high throughput sequencing.
Identification of SF-1 genomic binding sites in conditions of basal and increased dosage in the H295R adrenocortical tumor cell line
GEO Series GSE44220. Homo sapiens. 5 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Genome-wide SF-1 (NR5A1) binding sites in NCI-H295R cells
GEO Series GSE26144. Homo sapiens. 1 samples. Type: Genome binding/occupancy profiling by genome tiling array.
Identification of NRSF/REST genomic binding sites in conditions of basal and increased SF-1 dosage in the H295R adrenocortical tumor cell line
GEO Series GSE49014. Homo sapiens. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Identification of chromatin regulatory sites in conditions of basal and increased SF-1 dosage in the H295R adrenocortical tumor cell line
GEO Series GSE44221. Homo sapiens. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
MiRNA signatures in cell cycle sorted cells [H295R_miRNA_array]
GEO Series GSE73258. Homo sapiens. 8 samples. Type: Non-coding RNA profiling by array.
Effect of FSCN1 gene inactivation in the H295R cell line
GEO Series GSE216008. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
Angiotensin II early regulated genes in H295R human adrenocortical cells
GEO Series GSE1030. Homo sapiens. 16 samples. Type: Expression profiling by array.
Expression data from H295R cells in basal conditions/overexpressing SF-1
GEO Series GSE43032. Homo sapiens. 6 samples. Type: Expression profiling by array.
OH-PBDE-induced gene expression profiling in H295R adrenocortical carcinoma cells
GEO Series GSE8588. Homo sapiens. 9 samples. Type: Expression profiling by array.
H295R human adrenocortical cells treated with Angiotensin II, Potassium or Forskolin
GEO Series GSE5553. Homo sapiens. 10 samples. Type: Expression profiling by array.
MiRNA signatures in cell cycle sorted cells [HeLa_H295R_miRNA_seq]
GEO Series GSE73200. Homo sapiens. 9 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Bidirectional manipulation of SF-1 (NR5A1) in NCI-H295R cells
GEO Series GSE26529. Homo sapiens. 15 samples. Type: Expression profiling by array.
Expression data from H295R cells where NRSF/REST or SF-1 were knocked down
GEO Series GSE43033. Homo sapiens. 15 samples. Type: Expression profiling by array.
Chromatin profiling following SWI/SNF inhibition in H295R or HEK 293T cells [ChIP-seq]
GEO Series GSE260867. Homo sapiens. 25 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Transcriptional profiling of 8Br-cAMP treated human H295R adrenal cortex
GEO Series GSE32926. Homo sapiens. 23 samples. Type: Expression profiling by array.
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