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173 results for “HPLC”
Plum Island LTER phytoplankton identification using HPLC and Chem Taxonomy along transects in the Plum Island Sound estuary, Massachusetts. (Reformatted to the ecocomDP Design Pattern)
This data package is formatted as an ecocomDP (Ecological Community Data Pattern). For more information on ecocomDP see https://github.com/EDIorg/ecocomDP. This Level 1 data package was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-pie/404/4. The abstract below was extracted from the Level 0 data package and is included for context: Water column samples are collected along an estuarine salinity gradient as part of our monitoring surveys of the Parker River estuary each spring and late summer (typically high vs low freshwater input). Samples are filtered, and stored frozen for later pigment analyses by HPLC. Pigment data are then analyzed by CHEMTAX, calibrated to a matrix of pigment ratios based on taxonomy and enumeration of selected subsamples by microspcopy. Data are presented in terms of chlorophyll a concentrations partitionaed among the major phytoplankton groups as determined by CHEMTAX. For 2003-2006, sampling stations along the Plum Island Sound-Parker River were at fixed geographic locations at specific "Bends" in the river. In 2008, we began sampling the water column in salinity space rather than at specific geographic locations along the river. This sampling approach was adopted in order to follow particular water masses in this macrotidal estuary. In practical terms, it means that sampling locations, or stations, are not static. Therefore, we have mapped the 11 sampling locations (latitude and longitude are logged at each station) from each transect along the mainstem of the estuary, so each station may be placed along the river (to the nearest 0.5km) as well as in salinity space. We have also used the km marker to assign the sampling locations from each survey to one of four bounding boxes : the Sound (Plum Island Sound; EST-PR-SoundBND) which encompasses approximatly the first 9.5 km or the transect, with Okm at the mouth of the sound; the Lower Parker River (EST-PR-LowerParkerBND) , ~9.5 - 1
Plum Island LTER phytoplankton identification using HPLC and Chem Taxonomy along transects in the Plum Island Sound estuary, Massachusetts. (Reformatted to a Darwin Core Archive)
This data package is formatted as a Darwin Core Archive (DwC-A, event core). For more information on Darwin Core see https://www.tdwg.org/standards/dwc/. This Level 2 data package was derived from the Level 1 data package found here: https://pasta.lternet.edu/package/metadata/eml/edi/338/2, which was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-pie/404/4. The abstract below was extracted from the Level 0 data package and is included for context: Water column samples are collected along an estuarine salinity gradient as part of our monitoring surveys of the Parker River estuary each spring and late summer (typically high vs low freshwater input). Samples are filtered, and stored frozen for later pigment analyses by HPLC. Pigment data are then analyzed by CHEMTAX, calibrated to a matrix of pigment ratios based on taxonomy and enumeration of selected subsamples by microspcopy. Data are presented in terms of chlorophyll a concentrations partitionaed among the major phytoplankton groups as determined by CHEMTAX. For 2003-2006, sampling stations along the Plum Island Sound-Parker River were at fixed geographic locations at specific "Bends" in the river. In 2008, we began sampling the water column in salinity space rather than at specific geographic locations along the river. This sampling approach was adopted in order to follow particular water masses in this macrotidal estuary. In practical terms, it means that sampling locations, or stations, are not static. Therefore, we have mapped the 11 sampling locations (latitude and longitude are logged at each station) from each transect along the mainstem of the estuary, so each station may be placed along the river (to the nearest 0.5km) as well as in salinity space. We have also used the km marker to assign the sampling locations from each survey to one of four bounding boxes : the Sound (Plum Island Sound; EST-PR-SoundBND) which encompasses approximatly the first 9
Photosynthetic pigments of water column samples analyzed using High Performance Liquid Chromatography (HPLC), sampled during the Palmer LTER field seasons at Palmer Station, Antarctica, 1991 – 2023.
Phytoplankton pigment sampling was led by Prezelin from the 1991-1992 season through the 1993-1994 season, and then by Vernet from the 1994-1995 season through the 2006-2007 season. Schofield is the third, and current lead, beginning in the 2008-2009 season. Methods have been kept consistent as much as possible over the full time series and different Principal Investigators. Phytoplankton have a suite of accessory pigments in addition to Chlorophyll a, including other Chlorophyll’s (e.g. Chlorophyll b), Xanthophylls, and Carotenes. These accessory pigments can be used as chemotaxonomic markers to assess the composition and distribution of the phytoplankton community. For example, Fucoxanthin is a marker pigment of Diatoms, whereas Alloxanthin is a marker pigment of Cryptophytes. Accessory pigments also assist in photoacclimation and photoprotective processes. Water samples are collected throughout the water column at stations within the Palmer LTER region (primarily B and E, to 50m and 65m respectively). Water samples are filtered onto GF/F filters, and filters kept frozen at -80C until analysis. HPLC analysis is completed following Wright et al (1991). Following the guidelines set by NASA SeaHARRE, we use an internal standard and replicate injects on the HPLC to track recovery and replicability of the pigment extraction methods. Data is unavailable for the Palmer 2009-2010 season due to instrumentation problems and for the Palmer 2011-2012 season due to a freezer failure which resulted in the loss of samples. There is a temporary data gap for the Palmer 2015-2016, Palmer 2016-2017, Palmer 2019-2020, Palmer 2020-2021, and Palmer 2023-2024 seasons because those samples have not been analyzed yet.
Photosynthetic pigments of water column samples and analyzed with High Performance Liquid Chromatography (HPLC), collected aboard Palmer LTER annual cruises off the coast of the Western Antarctica Peninsula, 1991-2024.
Phytoplankton pigment sampling was led by Prezelin from 1991-1994, and then by Vernet from 1995-2008. Schofield is the third, and current lead, beginning in 2009. Methods have been kept consistent as much as possible over the full time series and different Principal Investigators. Phytoplankton have a suite of accessory pigments in addition to Chlorophyll a, including other Chlorophyll's (e.g. Chlorophyll b), Xanthophylls, and Carotenes. These accessory pigments can be used as chemotaxonomic markers to assess the composition and distribution of the phytoplankton community. For example, Fucoxanthin is a marker pigment of Diatoms, whereas Alloxanthin is a marker pigment of Cryptophytes. Accessory pigments also assist in photoacclimation and photoprotective processes. Water samples are collected throughout the water column along the Western Antarctic Peninsula at regular LTER grid stations where CTD casts are preformed and in surface waters at underway stations, where CTD casts are not done, using the ship's flow-through seawater system. Water samples are filtered onto GF/F filters, and filters kept frozen at -80C until analysis. HPLC analysis is completed following Wright et al (1991). Following the guidelines set by NASA SeaHARRE, we use an internal standard and replicate injects on the HPLC to track recovery and replicability of the pigment extraction methods and the HPLC. Data is unavailable for the LMG10-01 cruise due to instrumentation problems and for the LMG12-01 cruise due to a freezer failure which resulted in the loss of samples. There is no data for 2021 because there was no LTER cruise.
Plum Island LTER phytoplankton identification using HPLC and Chem Taxonomy along transects in the Plum Island Sound estuary, Massachusetts.
Water column samples are collected along an estuarine salinity gradient as part of our monitoring surveys of the Parker River estuary each spring and late summer (typically high vs low freshwater input). Samples are filtered, and stored frozen for later pigment analyses by HPLC. Pigment data are then analyzed by CHEMTAX, calibrated to a matrix of pigment ratios based on taxonomy and enumeration of selected subsamples by microspcopy. Data are presented in terms of chlorophyll a concentrations partitionaed among the major phytoplankton groups as determined by CHEMTAX. For 2003-2006, sampling stations along the Plum Island Sound-Parker River were at fixed geographic locations at specific "Bends" in the river. In 2008, we began sampling the water column in salinity space rather than at specific geographic locations along the river. This sampling approach was adopted in order to follow particular water masses in this macrotidal estuary. In practical terms, it means that sampling locations, or stations, are not static. Therefore, we have mapped the 11 sampling locations (latitude and longitude are logged at each station) from each transect along the mainstem of the estuary, so each station may be placed along the river (to the nearest 0.5km) as well as in salinity space. We have also used the km marker to assign the sampling locations from each survey to one of four bounding boxes : the Sound (Plum Island Sound; EST-PR-SoundBND) which encompasses approximatly the first 9.5 km or the transect, with Okm at the mouth of the sound; the Lower Parker River (EST-PR-LowerParkerBND) , ~9.5 - 14.5 km; the Middle Parker River (EST-PRMiddleParkerBND), ~14.5 - 18.75 km, and the Upper Parker River (EST-PR-UpperParker BND)., ~18.75 to 24.25 km (the Parker R. Dam).
Alkanna tinctoria (L.) Tausch roots in different soils and developmental stages, HPLC peak areas of alkannins/shikonins
<p>Table of peak areas of alkannins detected in the HPLC-UV/Vis analysis of extracts of <em>Alkanna tinctoria</em> (L.) Tausch root samples cultivated in the greenhouse in various soils. Samples come from four different stages of plant growth.</p>
Calibration-free reaction yield quantification by HPLC with a machine-learning model of extinction coefficients
<p>This repository contains all the data and code associated with the manuscript "Calibration-free reaction yield quantification by HPLC with a machine-learning model of extinction coefficients"</p> <p>Mass spec and absorption chromatogram data are in reaction_set_1.zip, reaction_set_2.zip, and simulated_reaction_set.zip. The chemprop model trained on the Deep4Chem dataset is in Deep4Chem_chemprop.zip.</p>
HPLC analysis of microalgal biomass produced in the ASTEASIER project
<p>HPLC analysis of microalgal samples produced in the framework of ASTEASIER project (WP1). The datasets include HPLC chromatogram, peaks reports and quantification results.</p> <p>Samples:</p> <p>H. pluvialis: Haematococcus pluvialis (biomass produced at the University of Verona in 300L scale)</p> <p>S4 Whole cells: Nannochloropsis gaditana S4 strain whole cells (biomass produced at A4F facilities in 5000L scale)</p> <p>S4 Broken cell: Nannochloropsis gaditana S4 strain broken cells (biomass produced at A4F facilities in 5000L scale)</p> <p>BC: Synechococcus PCC 11901 BC strain (biomass prodiced at A4F facilities in 1200L scale</p> <p> </p> <p>Peaks Reports: analysis of the chromatograms peaks. The information reported includes:</p> <p>HPLC System Name : HPLC instrument used </p> <p> Chromatogram Name : sample analyzed</p> <p> Acquisition Date: date of HPLC run </p> <p>tR : retention time </p> <p> Area : area of the peak </p> <p>Height : height of the peak </p> <p>Area% : percentage of the peak area compared to the overall chromatogram </p> <p>Height% : percentage of the peak heigth compared to the overall chromatogram </p> <p> Peak Width: width of the identified peak </p> <p>Sample # : number of the sample run</p> <p> Volume: volume of the sample injected</p>
Figure 6 in Potential Hepatoprotective Effect of Cheatomorpha gracilis extract against High Fat Diet (HFD)-Induced Liver Damage, and its characterization by HPLC
Figure 6. Photomicrographs of hepatocytes in control and experimental treated mice. Liver tissue sections stained with oil red O (×200) (A) and scored (B) by the semi-quantitative percentage of damaged area. (Control) control mice and (AEC) treated with C. gracilis extract (250 mg/kg) alone, showed normal structure. (HFD) high fed diet treated mice, showed a significant increase of lipid content; (AEC + HFD) co-treated with high fed diet and alga extract (250 mg/kg), representative micrograph revealed significantly reduced lipid liver content. Values are expressed as the mean ± SD. **p<0.01; ***p <0.001, indicates a significant difference compared to the control group. ++ p<0.01, indicates a significant difference compared to the HFD alone group.
Figure 3 in Potential Hepatoprotective Effect of Cheatomorpha gracilis extract against High Fat Diet (HFD)-Induced Liver Damage, and its characterization by HPLC
Figure 3. Effect of ACE supplementation on plasma levels of inflammatory proteins. Data are given as mean ± SD for group of eight mice each. Values are statistically presented as follows: HFD and AEC-treated groups vs. control group: *p <0.05; **p<0.01. HFD + AEC group vs. HFD group: +p <0.05; ++p<0.01.
Figure 2 in Potential Hepatoprotective Effect of Cheatomorpha gracilis extract against High Fat Diet (HFD)-Induced Liver Damage, and its characterization by HPLC
Figure 2. Antioxidant activity of different extracts of C. gracilis. Values are mean ± SD of three experimental tests, mg GAE/g DR: mg gallic acid equivalents per g dry residue. Table 4. Effect of AEC on body weight, liver and adipose tissues relative weight of control and treated mice groups.
Figure 5 in Potential Hepatoprotective Effect of Cheatomorpha gracilis extract against High Fat Diet (HFD)-Induced Liver Damage, and its characterization by HPLC
Figure 5. Photographs of liver tissues in control and experimental treated mice. Liver tissue sections stained with hematoxylin and eosin (original magnification × 400). (A), in control mice showing normal histoarchitecture and radiating cell arrangement. (B) In mice treated with aqueous extract of C.gracilis (AEC) (250mg/kg) alone showing the normal liver structure. (C) In High fed diet (HFD) mice, representative photograph shows a significant lipid accumulation. (D) In group co-treated with HFD and AEC, was observed the reduction of fat accumulation.
Figure 1 in Potential Hepatoprotective Effect of Cheatomorpha gracilis extract against High Fat Diet (HFD)-Induced Liver Damage, and its characterization by HPLC
Figure 1. Phenolic compounds of Cheatomorpha gracilis extract by chemical High Performance Liquid Chromatography (HPLC) analysis (gallic acid, Rutin, quercetin, Apegenin, kaempferol and Naringenin).
Figure 4 in Potential Hepatoprotective Effect of Cheatomorpha gracilis extract against High Fat Diet (HFD)-Induced Liver Damage, and its characterization by HPLC
Figure 4. Effects of C.gracilis aqueous extract (AEC) on TBARS and protein carbonyls products (PCO) in HFD-treated mice. TBARS: thiobarbituric acid reactive substance. Data are presented as mean ± SD, n =8. *P≤ 0.05, **P≤ 0.01 compared with the control group. +P ≤0.05, ++P ≤0.01 as compared with HFD- treated mice.
NMR, HPLC and HRMS data - General Cyclopropane Assembly via Enantioselective Transfer of a Redox-Active Carbene to Aliphatic Olefins
<p>Raw data for the article with the same title.</p> <p>ChemRxiv pre-print (<a href="https://doi.org/10.26434/chemrxiv.7436795">https://doi.org/10.26434/chemrxiv.7436795</a>)</p>
Dataset HPLC purification improves 68Ga-DOTA-NAPamide
<p>Numerical data for the manuscript "<strong>Increasing molar activity by HPLC purification improves <sup>68</sup>Ga-DOTA-NAPamide tumor accumulation in a B16/F1 melanoma xenograft model</strong>"</p>
Gradual Pressure Error in an HPLC-HRMS Measurement Run
<p>This dataset contains files of a reverse-phase HPLC-HRMS orbitrap measurement run during which the pump pressure gradually got more unstable. While a run was being recorded, the pump for the organic eluent ceased functioning slowly. This resulted in a high amount of new signals appearing in the form of "stripes" at specific retention times.</p> <p>It is intended as a control group for assigned data quality scores, with the reasoning that a highly unstable pressure in the chromatography will lead to more purely noise-induced signals. The relevant pressure profiles are also included. The mzML files were produced from the raw data using MSconvert and only contain the MS1 spectra.</p> <p>The last file in the series (<a href="https://zenodo.org/api/records/14046512/draft/files/20240805_AA_DK_Ibu_pH6_1min_20240808161549.raw/content" target="_blank" rel="noopener noreferrer">20240805_AA_DK_Ibu_pH6_1min_20240808161549</a>) remains incomplete since the instrument shut off halfway through the measurement.</p>
Simulated HPLC–DAD datasets saved as MOCCA campaigns
<p>Supplemental data for the publication: <strong>Open-Source Chromatographic Data Analysis for Reaction Optimization and Screening, </strong>submitted by the authors to<strong> </strong><em>ACS Central Science December 2022.</em></p> <p>The data set contains simulated HPLC–DAD datasets saved as MOCCA campaigns, which were used for validation and benchmarking. The files are saved as Python Pickle Files. The zip file contains all the simulated HPLC data one-step all-files download. </p>
Echium vulgare inoculated with fungal endophytes and arbuscular mycorrhizal fungi: HPLC data
<p>Raw HPLC data acquired on samples of <em>Echium vulgare</em> inoculated with fungal endophytes and arbuscular mycorrhizal fungi. Data were acquired on an ECOM HPLC system and are readable through the Clarity software (DataApex, Czech Republic). An .xlsx file is included which contains the descriptions of different treatments, i.e. the names of fungal species used for inoculation of various sample groups. Root and leachate samples were run separately, leachate samples are marked with "L" at the end of the sample name; the samples not containing this label are root samples.</p>
High Performance Liquid Chromatography (HPLC) pigment analysis from rosette bottle samples at various depths from CalCOFI-CCE Augmented cruises in the California Current System, 2002 to 2023 (ongoing).
High Performance Liquid Chromatography (HPLC) samples are collected from rosette bottles (from three to eight different depths in the photic zone) at stations located within the CCE region on CalCOFI cruises (since 2002, ongoing). The HPLC method is used to measure concentrations of chlorophylls and carotenoids in samples of particulate matter, which includes filtering and freezing the filter while at sea. The taxon-specific phyto-pigments are extracted back onshore. Concentrations of chlorophyll a are used as a proxy for phytoplankton biomass and concentrations of other taxon-specific pigments are used to determine contributions of phytoplankton taxa to total phytoplankton biomass.
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