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6 results for “HPLC pigment”
Photosynthetic pigments of water column samples analyzed using High Performance Liquid Chromatography (HPLC), sampled during the Palmer LTER field seasons at Palmer Station, Antarctica, 1991 – 2023.
Phytoplankton pigment sampling was led by Prezelin from the 1991-1992 season through the 1993-1994 season, and then by Vernet from the 1994-1995 season through the 2006-2007 season. Schofield is the third, and current lead, beginning in the 2008-2009 season. Methods have been kept consistent as much as possible over the full time series and different Principal Investigators. Phytoplankton have a suite of accessory pigments in addition to Chlorophyll a, including other Chlorophyll’s (e.g. Chlorophyll b), Xanthophylls, and Carotenes. These accessory pigments can be used as chemotaxonomic markers to assess the composition and distribution of the phytoplankton community. For example, Fucoxanthin is a marker pigment of Diatoms, whereas Alloxanthin is a marker pigment of Cryptophytes. Accessory pigments also assist in photoacclimation and photoprotective processes. Water samples are collected throughout the water column at stations within the Palmer LTER region (primarily B and E, to 50m and 65m respectively). Water samples are filtered onto GF/F filters, and filters kept frozen at -80C until analysis. HPLC analysis is completed following Wright et al (1991). Following the guidelines set by NASA SeaHARRE, we use an internal standard and replicate injects on the HPLC to track recovery and replicability of the pigment extraction methods. Data is unavailable for the Palmer 2009-2010 season due to instrumentation problems and for the Palmer 2011-2012 season due to a freezer failure which resulted in the loss of samples. There is a temporary data gap for the Palmer 2015-2016, Palmer 2016-2017, Palmer 2019-2020, Palmer 2020-2021, and Palmer 2023-2024 seasons because those samples have not been analyzed yet.
Photosynthetic pigments of water column samples and analyzed with High Performance Liquid Chromatography (HPLC), collected aboard Palmer LTER annual cruises off the coast of the Western Antarctica Peninsula, 1991-2024.
Phytoplankton pigment sampling was led by Prezelin from 1991-1994, and then by Vernet from 1995-2008. Schofield is the third, and current lead, beginning in 2009. Methods have been kept consistent as much as possible over the full time series and different Principal Investigators. Phytoplankton have a suite of accessory pigments in addition to Chlorophyll a, including other Chlorophyll's (e.g. Chlorophyll b), Xanthophylls, and Carotenes. These accessory pigments can be used as chemotaxonomic markers to assess the composition and distribution of the phytoplankton community. For example, Fucoxanthin is a marker pigment of Diatoms, whereas Alloxanthin is a marker pigment of Cryptophytes. Accessory pigments also assist in photoacclimation and photoprotective processes. Water samples are collected throughout the water column along the Western Antarctic Peninsula at regular LTER grid stations where CTD casts are preformed and in surface waters at underway stations, where CTD casts are not done, using the ship's flow-through seawater system. Water samples are filtered onto GF/F filters, and filters kept frozen at -80C until analysis. HPLC analysis is completed following Wright et al (1991). Following the guidelines set by NASA SeaHARRE, we use an internal standard and replicate injects on the HPLC to track recovery and replicability of the pigment extraction methods and the HPLC. Data is unavailable for the LMG10-01 cruise due to instrumentation problems and for the LMG12-01 cruise due to a freezer failure which resulted in the loss of samples. There is no data for 2021 because there was no LTER cruise.
High Performance Liquid Chromatography (HPLC) pigment analysis from rosette bottle samples at various depths from CalCOFI-CCE Augmented cruises in the California Current System, 2002 to 2023 (ongoing).
High Performance Liquid Chromatography (HPLC) samples are collected from rosette bottles (from three to eight different depths in the photic zone) at stations located within the CCE region on CalCOFI cruises (since 2002, ongoing). The HPLC method is used to measure concentrations of chlorophylls and carotenoids in samples of particulate matter, which includes filtering and freezing the filter while at sea. The taxon-specific phyto-pigments are extracted back onshore. Concentrations of chlorophyll a are used as a proxy for phytoplankton biomass and concentrations of other taxon-specific pigments are used to determine contributions of phytoplankton taxa to total phytoplankton biomass.
BiG RAPA Water Column HPLC Pigments from R/V Melville MV1015 in the South Pacific from Arica, Chile to Easter Island from November to December 2010 (C-MORE project)
<p>“The South East Pacific (SEP) is characterized by very high nutrient concentrations in the waters adjacent to the Chilean coast, but very low nutrient concentrations (oligotrophic) in the mid- South Pacific Subtropical Gyre (SPSG), near Easter Island. The steep gradient in nutrient concentrations across the region affects the level of marine production, the composition of the microbial community, and the operation of major biogeochemical cycles in ways that are not fully understood. Despite the remarkable diversity of trophic conditions, strong gradients and even some unique singularities, the SEP is still the most sparsely sampled oceanic region of the global ocean from hydrodynamic, biological and biogeochemical points of view. The SPSG is also the most oligotrophic of all sub-tropical gyres. Previous expeditions and remote sensing studies have describes the nutrient and chlorophyll field, but there have been few simultaneous measurements of chemical properties with microbial community structure and function. This expedition is designed to investigate the impact of elemental nutrient (nitrogen, phosphorus, iron, silicon, carbon) ratios on marine productivity and microbial community composition.” This BiG RAPA dataset contains water column HPLC pigment data from samples collected via Niskin bottles attached to a CTD. Time is in GMT.</p> <p><br> This description has been reproduced using the following source:</p> <p><br> http://dmoserv3.bco-dmo.org/jg/info/BCO-DMO/CMORE/bigrapa/pigments_watcol%7Bdir=dmoserv3.bco-dmo.org:80/jg/dir,data=dmoserv3.bco-dmo.org:80/jg/serv/BCO-DMO/CMORE/bigrapa/pigments_watcol.html1%7D?</p>
High Performance Liquid Chromatography (HPLC) pigment analysis from rosette bottle samples at various depths from CCE LTER process cruises in the California Current System, 2006 to 2017.
High Performance Liquid Chromatography (HPLC) samples are collected from rosette bottles (from three to eight different depths in the photic zone) at stations located within the CCE region on Process cruises (since 2006, ongoing). The HPLC method is used to measure concentrations of chlorophylls and carotenoids in samples of particulate matter, which includes filtering and freezing the filter while at sea. The taxon-specific phyto-pigments are extracted back onshore. Concentrations of chlorophyll a are used as a proxy for phytoplankton biomass and concentrations of other taxon-specific pigments are used to determine contributions of phytoplankton taxa to total phytoplankton biomass.
Pigment assemblages in subtropical bloom-forming cyanobacteria strains (HPLC data)
<p>We present the data associated to the article: Bonilla et al., Pigment assemblages in subtropical bloom-forming cyanobacteria strains (2023 accepted in Journal of Plankton Research). The table has the carotenoid ratios to chlorophyll a of nine subtropical cyanobacterial strains analysed by HPLC.</p>
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