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85 results for “HSPC”
Flow Cytometry data from: "The EMT transcription factor Zeb1 is essential for HSPC differentiation that acts synergistically with Zeb2 in fine-tuning hematopoietic lineage fidelity"
<p>Abstract:</p> <p>The Zeb2 transcription factor has been demonstrated to play important roles in hematopoiesis and leukemic transformation. Zeb1 is a close family member of Zeb2 but has remained more enigmatic concerning its roles in hematopoiesis. Here we show using conditional loss of function approaches and bone marrow reconstitution experiments that Zeb1 plays cell autonomous role in hematopoietic lineage differentiation, particularly as a positive regulator of monocyte development in addition to its previously reported important role in T-cell differentiation. Analysis of existing single cell RNAseq data of early hematopoiesis has revealed distinctive expression differences between Zeb1 and Zeb2 in HSPC differentiation with Zeb2 being more highly and broadly expressed that Zeb1 except at a key transition point (ST-HSCàMPP1) whereby Zeb1 appears to be the dominantly expressed family member. Inducible deletion of both Zeb1 and Zeb2 using a tamoxifen inducible Cre-mediated approach leads to acute bone marrow failure at this transition point with increased long-term and shortterm hematopoietic stem cell numbers and an accompanying decrease in all hematopoietic lineage differentiation. Bioinformatics analysis of RNAseq data has revealed that Zeb2 acts predominantly as a transcriptional repressor involved in restraining mature hematopoietic lineage gene expression programs from being expressed too early in hematopoietic stem and progenitor cells (HSPCs). Zeb1 appears to fine tune this repressive role during hematopoiesis to ensure hematopoietic lineage fidelity. Analysis of ROSA26 locus based transgenic models has revealed that Zeb1 as well as Zeb2 overexpression within the hematopoietic system can drive extramedullary hematopoiesis/splenomegaly and enhanced monocyte development. Finally, deletion of Zeb2 alone or Zeb1/2 together was found to enhance survival in secondary MLL-AF9 AML models attesting to the oncogenic role of Zeb1/2 in AML.</p> <p> </p> <p>Flow cytometric and Hematocrit analysis methods: </p> <p><br> Cells were stained with antibodies listed in the provided Supplemental Table (Antibodies.xlsx) according to the manufacturer guidelines. Flow cytometric analyses were performed on the LSRII and Fortessa X-20 cytometer (BD Biosciences) and the results were analysed by FACSDiva or FlowJo software (BD Biosciences). Cells for MLL-AF9 experiments and RNA-seq were stained and sorted on Influx or FACSAria Fusion sorters (BD Biosciences) at AMREP Flow Cytometry Core Facility and FlowCore, Monash University. <br> Submandibular blood samples were collected into EDTA-coated tubes, and hematology parameters were measured using a HemaVet 950FS automated blood analysis machine (Drew Scientific).</p>
Effects of Hyperbaric Oxygen (HBO) on Blood Count Recovery After Autologous Hematopoietic Stem Cell (HSPC) Transplant for Multiple Myeloma
ClinicalTrials.gov study NCT03398200. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
RNA sequestration in P-bodies sustains myeloid leukemia [HSPC_RNAseq]
GEO Series GSE274860. Mus musculus. 24 samples. Type: Expression profiling by high throughput sequencing.
EPCR Expression Defines the Most Primitive Subset of Human HSPC and Is Required for Their In Vivo Activity
GEO Series GSE77128. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome analysis to compare control and Ahed-deficient hematopoietic stem and progenitor cells (HSPC) and erythroblasts [RNA-Seq]
GEO Series GSE218517. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Differential expression of Hdc-GFPhi HSPC VS Hdc-GFPlo HSPC hematopoetic stem and progenitor cells from mouse bone marrow
GEO Series GSE80092. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
single cell analysis of hematopoietic stem/progenitor cells(HSPC) and microenvironmental cells from fetal mouse bone marrow
GEO Series GSE160741. Mus musculus. 2 samples. Type: Expression profiling by high throughput sequencing.
The neuronal homeobox transcription factor HMX3 is a crucial vulnerability in MECOM-negative KMT2A::MLLT3 acute myelomonocytic leukemia [RNAseq_CD34_HSPC_ectopic_HMX3]
GEO Series GSE242956. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Acute myeloid leukemia (AML) patient blasts and healthy hematopoietic stem and progenitor cell (HSPC) 3'RNA-Sequencing Data
GEO Series GSE146657. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
Inflammatory response in HSPC of PTPN11-mutated JMML
GEO Series GSE183252. Homo sapiens. 36 samples. Type: Expression profiling by high throughput sequencing.
Acute myeloid leukemia (AML) patient blasts and healthy hematopoietic stem and progenitor cell (HSPC) RNA-sequencing Data
GEO Series GSE149237. Homo sapiens. 13 samples. Type: Expression profiling by high throughput sequencing.
RNA-seq study on mouse bone marrow HSPC cells isolated from Arid2 conditional knockout and control mice.
GEO Series GSE164384. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome analysis of HSPC at 6 days of culture of non- or 20mGy-irradiated LT-HSC
GEO Series GSE286157. Mus musculus. 6 samples. Type: Expression profiling by array.
Expansion of in vivo HDAd-transduced HSPCs by constitutive expression of tHMGA2 without additional treatment allows for therapeutically relevant transgene expression in HSPC progeny cells without clon
GEO Series GSE268678. Mus musculus. 18 samples. Type: Other.
Allogeneic HSPC-engineered CD33-targeting CAR-NKT cells synergize with hypomethylating agents for effective and safe treatment of myeloid malignancies
GEO Series GSE270430. Homo sapiens. 4 samples. Type: Expression profiling by high throughput sequencing.
Expression analysis of Mll3 knockdown p53-/- HSPC vs. control p53-/- HSPC
GEO Series GSE54313. Mus musculus. 8 samples. Type: Expression profiling by array.
ETV6 Represses Inflammatory Response Genes and Regulates HSPC Function During Stress Hematopoiesis in Mice [RNA-seq]
GEO Series GSE213596. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
HSPC scRNA seq
GEO Series GSE149249. Mus musculus. 3 samples. Type: Expression profiling by high throughput sequencing; Other.
Leukemic initiation in Jam-C-deficient HSPC reveals AP-1/TNF- gene expression signature as a biomarker for AML
GEO Series GSE235693. Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.
Global Identification of the miR-130a targetome in human HSPC Reveals a Novel Role for TBL1XR1 in Hematopoietic Stem Cell Self-Renewal and t(8;21) AML
GEO Series GSE180129. Homo sapiens. 9 samples. Type: Other.
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International Brain Laboratory public data
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OpenNeuro
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