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12 results for “Halichoerus”
Fig. 1. A in A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus)
Fig. 1. A: graphical representation of cluster 20 (C20). B: representation of the selected contig and the sequence used to design the C20 quantitative real-time PCR assay. The forward primer is in bold, the reverse primer is indicated by a dotted underline, and the double-quenched probe is underlined.
Fig. 2. Standard curve generated using the log10 in A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus)
Fig. 2. Standard curve generated using the log10 of the ng of input A. spirocauda DNA plotted against Ct value. Unknown values are displayed as stars. For unknown samples, the total input DNA was 1 ng, which contains a mixture of seal DNA from the blood and A. spirocauda DNA. R2 = 0.985 for linear fit of standards. Curve is described by the equation y = −5.63x + 17.16, where y is the log (ng) and x is the Ct value. 95% confidence intervals are denoted by the dotted lines.
Figure 2 in Onset and duration of gray seal (Halichoerus grypus) molt in the Wadden Sea, and the role of environmental conditions
Figure 2. To aid interpretation of the data, this figure presents a progression of data processing in a simulation. (A) Observations of potential absolute seal numbers on a sandbank against day of the year, using assumptions described in the text. (B) The relative proportions (prop.) of seals in each molt phase changes over time. (C) The proportion of seals in pre-visible-molt (light blue) decreases over time and the proportion in post-molt increases, the seals in early- and late-molt (green lines) peak at certain times. (D) Over time, the numbers of seals on the sandbanks that are in each visible molt phase (i.e., excludes seals in pre- and post-molt), (E) Over time, the proportions of seals in visible molt that are in early- or late-molt. (F) The proportion of seals in visible molt that were in late-molt. Note that the change over time in this proportion is linear (the horizontal dashed line indicates 50% in each stage, which occurs in this simulation on day 105).
Figure 4 in Onset and duration of gray seal (Halichoerus grypus) molt in the Wadden Sea, and the role of environmental conditions
Figure 4. The proportion of wild gray seals (given an observation quality = 1) in different molt stages over time. The proportion in pre-visible-molt decreases and that in post-molt increases over time, while numbers in early- and late-molt peak during the molt period. Data are represented by the colored dots, the lines indicate the simulation of Fig. 2c.
Figure 6 in Onset and duration of gray seal (Halichoerus grypus) molt in the Wadden Sea, and the role of environmental conditions
Figure 6. Simulations of changes in the proportion of seals in visible molt that were in latemolt, compared to the base scenario (black). These figures show the effect of shorter (red) and longer visible (orange) molt durations (A); shorter early-molt (light green) or shorter late-molt (dark green) durations (B); changes in the day of onset of molt (C); changes in the variability between individuals (SD) (D); and changes in the haul-out percentage during early- and latemolt (E). The base scenario was derived from the parameters from captive seals identified in this study, i.e., visible molt commences on average on day 99, early-molt lasts 8.2 d and late-molt lasts 8.7 d. The standard deviation that defines the variability between individuals is 15 d. This base senario assumed that the haul-out behavior is the same during early- and late-molt.
Figure 1 in Onset and duration of gray seal (Halichoerus grypus) molt in the Wadden Sea, and the role of environmental conditions
Figure 1. Aerial photograph of molting gray seals on a Wadden Sea sandbank. Seals are in pre-visible-molt (A), early-molt (B), late-molt (C), or post-molt (D). Recognizable adult males were noted separately. The quality was not always consistent due to glare or sand, and therefore seals were defined to have high (1), moderate (2), bad (3), or inadequate quality (4). The contrast of the pelage color in the true picture (upper image) was automatically selected using color select in Adobe Photoshop CS (lower image). The brown old fur was colored orange, and the grayish new fur was colored blue showing an even stronger contrast between early- and late-molt.
Figure 3 in Onset and duration of gray seal (Halichoerus grypus) molt in the Wadden Sea, and the role of environmental conditions
Figure 3. The molt progress of nine captive gray seals recorded daily in 2010. The individuals were kept at two locations (Dolfinarium and Ecomare), had different origins, ages, and sexes (see y-axis). Note that the onset and end of molt varied per individual but the duration of the phases in the visible molt (early-molt and late-molt) were similar.
Data on three Baltic species of Corynosoma Lühe, 1905 (Acanthocephala: Polymorphidae) from Baltic grey (Halichoerus grypus) and ringed seals (Pusa hispida)
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Utilizing next-generation sequencing to identify prey DNA in western North Atlantic grey seal (Halichoerus grypus) diet
<p>Increasing grey seal (<i>Halichoerus grypus</i>) abundance in coastal New England is leading to social, political, economic, and ecological controversies. We studied grey seal feeding habits through next-generation sequencing of prey DNA using 16S amplicons from seal scat (N = 74) collected from a breeding colony on Monomoy Island in Massachusetts, U.S. and report frequency of occurrence and relative read abundance. We also assigned seal sex to scat samples using a revised PCR assay. In contrast to current understanding of grey seal diet from hard parts and fatty acid analysis, we found no significant difference between male and female diet measured by alpha and beta diversity. Overall, we detected 24 prey groups, 18 of which resolved to species. Sand lance (<i>Ammodytes</i> spp.) was the most frequently consumed prey group, with a frequency of occurrence (FO) of 97.3%, consistent with previous studies, but Atlantic menhaden (<i>Brevoortia tyrannus</i>), the second most frequently consumed species (FO = 60.8%), has not been documented in U.S. grey seal diet previously. Our results suggest that a metabarcoding approach to seal food habits can yield important new ecological insights, but that traditional hard parts analysis does not underestimate consumption of Atlantic cod (<i>Gadus morhua; </i>FO =<i> </i>6.7% Gadidae spp.) and salmon (<i>Salmo salar; </i>FO = 0%), two particularly valuable species of concern.</p>
Data from: Geographic variation of the major histocompatibility complex in Eastern Atlantic grey seals (Halichoerus grypus)
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Utilizing next-generation sequencing to identify prey DNA in western North Atlantic grey seal (Halichoerus grypus) diet
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Figure 5 in Onset and duration of gray seal (Halichoerus grypus) molt in the Wadden Sea, and the role of environmental conditions
Figure 5. The proportion of visible molting seals in late-molt over time for the years between 2004 and 2010. The onset of molt varied per year: the earliest onset in 2009 and the latest onset in 2008. The bubble size indicates the number of animals with an observation quality score of 1 for each survey. The dashed horizontal line shows the 50% level and the vertical bold red and black dashed lines show at what day of the year 50% of the visible molting animals were in late-molt, respectively.
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