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74 results for “Helotiales”
Text-fig. 5. Original material of Peziza sulphurea (syntype L 910,261-594), the substrate and schema with location of six apothecia and two fragments of apothecia. Apothecia marked with numbers 1–3 were examined microscopically. in A Revision Of Trichopeziza Lizonii, T. Sulphurea And T. Violascens (Ascomycota, Helotiales) From The Herbarium Prm With Notes On Type Material Of Peziza Sulphurea
Text-fig. 5. Original material of Peziza sulphurea (syntype L 910,261-594), the substrate and schema with location of six apothecia and two fragments of apothecia. Apothecia marked with numbers 1–3 were examined microscopically.
Text-fig. 3. Trichopeziza sulphurea (PRM 672828, epitype). a. hairs; b. ascospores; c. asci; d. asci arising from simple septa; e. paraphyses. Scale bars: a, b, c-d, e = 10 µm. in A Revision Of Trichopeziza Lizonii, T. Sulphurea And T. Violascens (Ascomycota, Helotiales) From The Herbarium Prm With Notes On Type Material Of Peziza Sulphurea
Text-fig. 3. Trichopeziza sulphurea (PRM 672828, epitype). a. hairs; b. ascospores; c. asci; d. asci arising from simple septa; e. paraphyses. Scale bars: a, b, c-d, e = 10 µm.
Text-fig. 4. Trichopeziza violascens (PRM 915197). a. ascospores; b. hairs; c. paraphyses; d. asci; e. young asci with croziers. Scale bars: a, b, c, d-e = 10 µm. in A Revision Of Trichopeziza Lizonii, T. Sulphurea And T. Violascens (Ascomycota, Helotiales) From The Herbarium Prm With Notes On Type Material Of Peziza Sulphurea
Text-fig. 4. Trichopeziza violascens (PRM 915197). a. ascospores; b. hairs; c. paraphyses; d. asci; e. young asci with croziers. Scale bars: a, b, c, d-e = 10 µm.
Comparative genomics of Helotiales (Leotiomycetes) and in silico analysis of temperature adaptations of plant-associated genes
<p><span>Table S1: Genome assembly size and quality for every species in this study; </span></p> <p><span>Table S2: Genome annotation counts for P450, virulence factors, effectors, and CAZy genes and their temperature adaptation.</span></p>
Supplementary material 5 from: Tochihara Y, Hosoya T (2022) Examination of the generic concept and species boundaries of the genus Erioscyphella (Lachnaceae, Helotiales, Ascomycota) with the proposal of new species and new combinations based on the Japanese materials. MycoKeys 87: 1-52. https://doi.org/10.3897/mycokeys.87.73082
Figure S5. ML best-scored phylogenetic tree based on concatenated dataset of ITS1, 5.8S, and ITS2 constructed by RAxML-NG
FIGURE 5 in Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales)
FIGURE 5. Calycina shangrilana (HMAS 275568, holotype). a A herbarium package b–c Apothecia on the woody substrate. d Cross sections of an apothecium. e Close up of hymenium at the margin. f Close up of ectal excipulum cells. g Filiform paraphyses (mounted in Congo red). h–j Asci (h, i mounted in Congo red, j mounted in Melzer's agent). k Tip of the ascus (J+, in Melzer's agent with KOH treatment). l Close up of croziers at the base of an immature ascus. m–n Ascospores (mounted in Congo red). Scale bars: b–c = 475 μm, d = 350 μm, e = 100 μm, f = 15 μm, g = 20 μm, h–j = 17 μm, k = 5 μm, l–n = 9 μm.
FIGURE 3 in Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales)
FIGURE 3. Calycina montana (MFLU 22-0055, new geographical record). a Close up of excipulum cells. b Ectal excipulum cells (b mounted in Congo Red). c Medullary excipulum cells. d–e Filiform paraphyses with refractive guttules. f–g Asci (mounted in Congo red). h Asci (alive state). i Close up of crozier. j Tip of the ascus (J+, in Melzerr's agent with KOH treatment). k–m Ascospores (m mounted in Congo red). Scale bars: a = 40 μm, b–c = 25 μm, d–h = 14 μm, i = 10 μm, j–m = 4 μm.
FIGURE 1 in Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales)
FIGURE 1. The phylogram of combined ITS and LSU sequence data for genera in Pezizellaceae. Maximum likelihood bootstrap values greater than 70% and posterior probability values greater than 0.95 given near the nodes. The new geographical record highlighted in red and type strains are in bold. The tree is rooted to Chlorencoelia torta (JAC 14068, KUSF 52256 and ICMP 21732).
FIGURE 2 in Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales)
FIGURE 2. Calycina montana (MFLU 22-0055, new geographical record). a Rotten wood substrates covered with mosses. b–c Apothecia on the substrate. d Close-up of an apothecium sectioning. e Close-up of hymenium at the margin. f Phialide cells on lower flank surface. g–h Conidiogenous cells bearing conidiospore i–j A conidiogenous cell (j Mounted in Congo red). Scale bars: b = 950 μm, c = 470 μm, d = 315 μm, e = 141 μm, f = 40 μm, g = 17 μm, h–j = 13 μm.
FIGURE 4 in Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales)
FIGURE 4. Calycina montana (HMAS 275566, holotype). a A herbarium package. b An apothecium on the woody substrate. c–d Cross sections of an apothecium (d mounted in Congo red). e Close up of hymenium at the margin. f Close up of ectal excipulum cells. g–h Filiform paraphyses (dead state). i–l Asci (k mounted in Melzer's agent, l mounted in Congo red). m Tip of the ascus (J+, in Melzer's agent with KOH treatment). n Close up of crozier at the base of an immature ascus. o–p Ascospores (o mounted in Congo red). Scale bars: b = 500 μm, c–d = 380 μm, e = 43 μm, f = 25 μm, g–h = 23 μm, i–l = 19 μm, m = 4 μm, n = 8 μm, m, o–p = 4 μm.
FIGURE 2 in Unveiling the Diversity: A Novel Species of Dicephalospora (Helotiaceae, Helotiales) Discovered in China
FIGURE 2. Dicephalospora xishuangbannaensis (HKAS 131164, holotype). a Fresh specimen growing on a dead wood; b Cross section of the apothecium; c–e Close-up of the ectal excipulum and medullary excipulum cells; f–g Paraphyses; h–l Immature to mature asci (l treated with Melzer's reagent); m, n Apices of asci treated with Melzer's reagent; o–t Ascospores. Scale bars: b = 200 µm; c = 100 µm; d, f–l = 50 µm; e, m–n = 20 µm; o–t = 10 µm.
FIGURE 1 in Unveiling the Diversity: A Novel Species of Dicephalospora (Helotiaceae, Helotiales) Discovered in China
FIGURE 1. Phylogram of RAxML analysis based on the combined LSU and ITS sequence dataset. Bootstrap support values for maximum likelihood equal to or greater than 50%, and Bayesian posterior probability equal to or greater than 0.95 BYPP are indicated at the nodes as ML/BYPP. The generic names were denoted in the right side of tree. Type strains are in bold. The newly generated isolates are in red.
FIGURE 2. Macroskyttea parmotrematis. A in Macroskyttea parmotrematis gen. et sp. nov. (Helotiales, Leotiomycetes, Ascomycota), a new lichenicolous fungus from Bolivia
FIGURE 2. Macroskyttea parmotrematis. A, habit of a ascomata: A, young almost close ascomata (Kukwa 11316), B, mature ascoma with strongly exposed dark pigmented disc (Bach et al. 97a); C, cross section of mature ascoma (Bach et al. 97a); D, cross section of young ascoma showing excipular hairs (Kukwa 11316); E, ascospores (Kukwa 11316). Scale bars: A–B—250 μm, C—50 μm, D—25 μm, E—10 μm.
FIGURE 1 in Macroskyttea parmotrematis gen. et sp. nov. (Helotiales, Leotiomycetes, Ascomycota), a new lichenicolous fungus from Bolivia
FIGURE 1. Three-locus (nuSSU, nuLSU and 5.8S of ITS) Maximum Likelihood tree indicating phylogenetic position of Macroskyttea parmotrematis within encoelioid-clade of Helotiales. The branches supported by Maximum Likelihood and Bayesian analysed are marked with thicker lines, * indicates support in one analyse. Bootstrap values (BP)≥than 70 and Bayesian posterior probabilities (PP)≥than 0.95 are indicated above the branches.
FIGURE 3. Macroskyttea parmotrematis. A in Macroskyttea parmotrematis gen. et sp. nov. (Helotiales, Leotiomycetes, Ascomycota), a new lichenicolous fungus from Bolivia
FIGURE 3. Macroskyttea parmotrematis. A, cross section of mature ascoma mounted in LPCB (Bach et al. 97a); B, cross section of young ascoma mounted in LPCB (Etayo 28402); C, cross section of young ascoma mounted in LPCB showing inner (left) and ectal (right) exciple (Etayo 28402); D, inner exciple mounted in LPCB showing thin-walled cells (structura angularis) (Etayo 28402); E, ectal exciple mounted in LPCB showing thick-walled cells (structura globosa) (Etayo 28402); F, asci in different development stages mounted in Congo Red showing apically thickened tholus with a narrow ocular chamber with a characteristic widening on top, two right-hand asci with 4-guttulate ascospores (Etayo 28402). Scale bars: A–B—25 μm, C–F—10 μm.
FIGURE 3 in Calycina alstrupii sp. nov. (Pezizellaceae, Helotiales), a new lichenicolous fungus from Norway
FIGURE 3. Calycina alstrupii (holotype, BILAS 10761). a, b ascomata on the underside of Lobaria pulmonaria thallus (scale in mm); c hyphoid-type excipular hyphae in K/I; d single ascus with ascospores and an ascospore in water; e asci and paraphyses pre-treated with K/I (c–e scale in μm); f Calycina-type apical apparatus observed in K/I.
FIGURE 1 in Calycina alstrupii sp. nov. (Pezizellaceae, Helotiales), a new lichenicolous fungus from Norway
FIGURE 1. Two locus (nuLSU+mtSSU) 50% majority rule consensus tree of selected members of Helotiales based on Bayesian approach and showing the position of Calycina alstrupii. The branches with posterior probabilities (PP) ≥ 0.95 are considered as supported. The abbreviations Pz162 and Pz167 at the tips of C. alstrupii denote lab codes of holo- and paratype respectively.
FIGURE 2. A in Calycina alstrupii sp. nov. (Pezizellaceae, Helotiales), a new lichenicolous fungus from Norway
FIGURE 2. A Bayesian phylogeny based on rDNA ITS sequences of selected members of Hyaloscyphaceae s. lato and showing Calycina alstrupii within Calycina-clade. The branches with posterior probabilities (PP) ≥ 0.95 are considered as supported. The abbreviations Pz162 and Pz167 at the tips of C. alstrupii denote lab codes of holo- and paratype respectively.
FIGURE 2 in A new species of Chlorociboria (Helotiales, Ascomycota) on herbaceous stems from China
FIGURE 2. Maximum parsimony tree inferred from combined dataset of ITS and LSU showing the phylogenetic position of Chlorociboria herbicola. Bootstrap values (>50%) of maximum parsimony and neighbor-joining are indicated at the nodes from left to right.
FIGURE 1. Chlorociboria herbicola H.D. Zheng & W.Y in A new species of Chlorociboria (Helotiales, Ascomycota) on herbaceous stems from China
FIGURE 1. Chlorociboria herbicola H.D. Zheng & W.Y. Zhuang (HMAS 273905). A: Fresh apothecia on natural substrate. B: Dry apothecium (upper surface). C: Dry apothecium (lower surface). D: Partial rehydrated apotheium (lower surface); E: Longitudinal section of apothecium. F: Structure of margin. G: Excipular structure of flank. H: Asci. I: Croziers at ascus bases. J: IKI reaction of apical rings. K & L: Ascospores. Mounting media: D water; E–I, K lactophenol cotton blue; J & L Melzer's reagent. Bars: A 2 mm; B–D 0.2 mm; E 100 μm; F & G 20 μm; H 10 μm; I–L 5 μm.
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