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12 results for “Henlea”
Fig. 10 in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 10 Phylogeny of Henlea based on combined COI, H3, 12S, 16S, 18S, and 28S data from 13 Henlea species and five outgroups estimated using Bayesian Inference in MrBayes. The specimen shaded in red has a
Fig. 3 in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 3 Henlea petushkovi sp. n. Different views of prostomium in living worms. a dorsal. b lateral. c dorsolateral. d ventrolateral. Scale bars: a–d 200 μm
Fig. 1 in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 1 Bioluminescent Henlea specimen photographed in vivo. a Under a light microscope. b By direct contact printing worm-to-film in the dark room (courtesy of V. N. Petushkov & N. S. Rodionova). Scale bar: 1 mm
Fig. 4 in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 4 Henlea petushkovi sp. n. Internal anatomy shown by histological sections. a Longitudinal oblique section of segments V–IX (arrows point to the branches of oesophageal appendages in VI and VII). b Longitudinal parasagittal section through the gut diverticula in VIII, showing the anterior connection to septum 7/8. c–e Longitudinal sections through a spermatheca, showing: c the glands (gl) surrounding the distal end of ectal duct; d the bell- shaped widening of the ectal duct canal at the junction with ampulla; e the ampulla and ental duct joining gut close to septum 5/ 6. f Sperm funnel. g, h Arrangement of clitellar glands, from tangential sections of XII (g dorsal) and XI–XIII (h ventral). Scale bars: a, b, h 200 μm; c–g 100 μm
Fig. 7 in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 7 Henlea rodionovae sp. n. Internal anatomy as seen in living worms. a, b Dorsal view of segments VI–X and close-up of gut diverticula in VIII. c, d Ventral view of segments VI–X and close-up of gut diverticula in VIII. e, f Dorsal and ventral views of oesophageal appendages and their branches (arrows) in VI. g Close-up of nephridium (black arrow, nephrostome; white arrow, efferent duct) and coelomocytes (cc). h Spermatheca. j Sperm funnel. Scale bars: a–j 200 μm
Fig. 6 in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 6 Henlea rodionovae sp. n. Morphology of prostomium and body wall in living worms. a–d, f, g Different views of prostomium (a, g dorsal. b, c ventral. d, f ventrolateral). e Body wall of segments III–V. Arrows point to rows of epidermal glands. Scale bars: a–g 200 μm
Fig. 2 in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 2 Histological cross sections of the gut diverticula in Enchytraeus leptodera Vejdovský (a) and Enchytraeus ventriculosus d'Udekem (b), and in Archienchytraeus nasutus Eisen (c), as shown by Michaelsen (1886) and Michaelsen (1889), respectively
Fig. 5 in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 5 Henlea petushkovi sp. n. Internal anatomy as seen in living worms. a–c Different views of gut diverticula in VIII (a dorsolateral. b dorsal. c ventrolateral); white arrows indicate the thickened septum 7/8. d Close-up of nephridium and coelomocytes. Scale bars: a–d 200 μm
Fig. 9 Single gene trees and haplotype networks. a, b in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 9 Single gene trees and haplotype networks. a, b Gene-trees (a COI, b H3) estimated with Bayesian coalescent analysis in BEAST. Numbers above branches are posterior probabilities. Scales show expected numbers of substitution per site. The specimen shaded in red has a conflict between morphology and COI data. c, d Statistical parsimony
Fig. 8 in Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae)
Fig. 8 Henlea sp. "A", living worm. a Ventral view of segments I–XIV (white arrow, oesophageal appendage). b, c Prostomium, showing its peculiar sinuous furrow. d, e Close-up of oesophageal appendages forming a thick middorsal loop in anterior of V (d within circle) and giving off branches in VI (e). f Coelomocytes. g Gut diverticula in VIII. h Ventral chaetal bundle in a postclitellar segment. Scale bars: a 500 μm, b–h 200 μm
Fig. 2. Henlea montana. A in New record of three aquatic species of Enchytraeidae (Annelida: Clitellata) from Korea
Fig. 2. Henlea montana. A. shape of brain; B. anterior body region; C. spermatheca; D. chaetae per bundles; E. coelomocyte; F. clitellum (Scale bars: A = 50 μm, B = 100 μm, C-F = 20 μm).
Supporting Information for "Henlea earthworm bioluminescence comprises violet-blue BRET from tryptophan 2-carboxylate to deazaflavin cofactor"
<p>The dataset contains:</p> <ol> <li>Raw NMR spectra of tryptophan 2-carboxylate (1a) and its acetylated derivative (1b) obtained on Bruker NMR spectrometers</li> <li>MS2 fragmentation patterns of synthetic and natural (Henlea sp. extracts) 1a and 1b (Figure 1C,D of the article published)</li> <li>Chromatography, UV spectra and excitation/emissin spectra used in Figure 2</li> <li>Excitation, emission and bioluminescence spectra from Figure 3</li> </ol> <p>Please cite the main peer-reviewed article as follows:</p> <p>[1] V.N. Petushkov, M.V. Vavilov, A.N. Khokhlova, R.I. Zagitova, O.A. Belozerova, A.S. Shcheglov, S.I. Kovalchuk, A.S. Tsarkova, N.S. Rodionova, I.V. Yampolsky, M.A. Dubinnyi, <em>Henlea</em> earthworm bioluminescence comprises violet-blue BRET from tryptophan 2-carboxylate to deazaflavin cofactor, Biochemical and Biophysical Research Communications 708 (2024) 149787. <a href="https://doi.org/10.1016/j.bbrc.2024.149787">https://doi.org/10.1016/j.bbrc.2024.149787</a>.</p>
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