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40 results for “Hepatitis E virus”
Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein
<p>Hepatitis E virus (HEV) is one of the most common causes of acute hepatitis and jaundice in the world. Current understanding of the molecular virology and pathogenesis of hepatitis E is incomplete, especially due to the limited availability of functional tools. Here, we report the development of tagged HEV genomes as a novel tool to investigate the viral life cycle. A selectable subgenomic HEV replicon was subjected to random 15-nucleotide sequence insertion using transposon-based technology. Viable insertions in the open reading frame 1 (ORF1) protein were selected in a hepatoblastoma cell line. Functional insertion sites were identified downstream of the methyltransferase domain, in the hypervariable region (HVR) and between the helicase and RNA-dependent RNA polymerase domains. HEV genomes harboring a hemagglutinin (HA) epitope tag or a small luciferase (NanoLuc) in the HVR were found to be fully functional and to allow for the production of infectious virus. NanoLuc allowed to quantitatively monitor HEV infection and replication by luciferase assay. HA-tagged replicons and full-length genomes allowed to localize putative sites of HEV RNA replication by the simultaneous detection of viral RNA by fluorescence in situ hybridization and of ORF1 protein by immunofluorescence. Candidate HEV replication complexes were found in cytoplasmic dot-like structures which partially overlap with ORF2 and ORF3 proteins as well as exosomal markers. Hence, tagged HEV genomes yield new insights into the viral life cycle and should allow to further investigate the structure and composition of the viral replication complex.</p>
"Palmitoylation Mediates Membrane Association of Hepatitis E Virus ORF3 Protein and is Required for Infectious Particle Secretion"
<p><strong>Hepatitis E virus (HEV) is a positive-strand RNA virus encoding 3 open reading frames (ORF). HEV ORF3 protein is a small, hitherto poorly characterized protein involved in viral particle secretion and possibly other functions. Here, we show that HEV ORF3 protein forms membrane-associated oligomers. Immunoblot analyses of ORF3 protein expressed in cell-free <em>vs</em>. cellular systems suggested a posttranslational modification. Further analyses revealed that HEV ORF3 protein is palmitoylated at cysteine residues in its N-terminal region, as corroborated by <sup>3</sup>H-palmitate labeling, the investigation of cysteine-to-alanine substitution mutants and treatment with the palmitoylation inhibitor 2-bromopalmitate (2-BP). Abrogation of palmitoylation by site-directed mutagenesis or 2-BP treatment altered the subcellular localization of ORF3 protein, reduced the stability of the protein and strongly impaired the secretion of infectious particles. </strong><strong>Moreover, selective membrane permeabilization coupled with immunofluorescence microscopy revealed that HEV ORF3 protein is entirely exposed to the cytosolic side of the membrane, allowing to propose a model for its membrane topology and interactions required in the viral life cycle. </strong><strong>In conclusion, palmitoylation determines the subcellular localization, membrane topology and function of HEV ORF3 protein in the HEV life cycle. </strong></p>
A Study of JNJ 73763989+JNJ 56136379+Nucleos(t)Ide Analog (NA) Regimen Compared to NA Alone in e Antigen Negative Virologically Suppressed Participants With Chronic Hepatitis B Virus Infection
ClinicalTrials.gov study NCT04129554. IPD Sharing: YES. Countries: 7. Publications: 1.
Host Responses in Kidney-transplant Recipients With Chronic Hepatitis E Virus Infection
ClinicalTrials.gov study NCT01090232. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Role of Cytokines in Hepatitis E Virus Infection During Pregnancy
ClinicalTrials.gov study NCT01062321. IPD Sharing: Not stated. Countries: 1. Publications: 5.
Data from: Epidemiology of Hepatitis E Virus in China: results from the Third National Viral Hepatitis Prevalence Survey, 2005-2006
Open the record for dataset details and reuse information.
Morbidity and Mortality of Hepatitis E Virus Infections in Belgium
ClinicalTrials.gov study NCT04670419. IPD Sharing: NO. Countries: 0. Publications: 1.
A Multi-dose Study of ARC-520 in Patients With Hepatitis B 'e' Antigen (HBeAg) Negative, Chronic Hepatitis B Virus (HBV) Infection
ClinicalTrials.gov study NCT02604199. IPD Sharing: Not stated. Countries: 3. Publications: 0.
Study of ARC-520 in Participants With Hepatitis B Virus e Antigen (HBeAg) Positive Chronic Hepatitis B Virus
ClinicalTrials.gov study NCT02452528. IPD Sharing: Not stated. Countries: 1. Publications: 0.
A Multi-dose Study of ARC-520 in Patients With Hepatitis B 'e' Antigen (HBeAg) Positive, Chronic Hepatitis B Virus Infection
ClinicalTrials.gov study NCT02604212. IPD Sharing: Not stated. Countries: 3. Publications: 0.
Targeting cholesterol metabolism as efficient antiviral strategy against the Hepatitis E virus
GEO Series GSE157820. Homo sapiens. 12 samples. Type: Expression profiling by array.
Real-time quantitative PCR analysis of human HepaRG cells infected with hepatitis E virus (HEV) [D100 plaque 1]
GEO Series GSE243856. Homo sapiens. 12 samples. Type: Expression profiling by RT-PCR.
The hepatitis E virus capsid protein ORF2 counteracts cell-intrinsic antiviral responses to enable persistence in hepatocytes
GEO Series GSE288400. Homo sapiens. 5 samples. Type: Expression profiling by high throughput sequencing.
Real-time quantitative PCR analysis of liver tissue from pigs infected experimentally with hepatitis E virus (HEV) [pig liver]
GEO Series GSE243862. Sus scrofa. 8 samples. Type: Expression profiling by RT-PCR.
Modeling extrahepatic hepatitis E virus infection in induced human primary neurons
GEO Series GSE275473. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
Extrahepatic replication and genomic signatures of the hepatitis E virus in the kidney
GEO Series GSE300759. Hepatitis E virus. 11 samples. Type: Other.
Real-time quantitative PCR analysis of human HepaRG cells infected with hepatitis E virus (HEV) [pre-array]
GEO Series GSE243855. Homo sapiens. 20 samples. Type: Expression profiling by RT-PCR.
Real-time quantitative PCR analysis of human HepaRG cells infected with hepatitis E virus (HEV) [D100 plaque 2]
GEO Series GSE243860. Homo sapiens. 12 samples. Type: Expression profiling by RT-PCR.
Evaluating the Safety and Efficacy of Inarigivir in Non-cirrhotic, Hepatitis B e Antigen-negative Subjects Infected With HBV Virus and Receiving or Stopping Treatment With a NUC Inhibitor
ClinicalTrials.gov study NCT04023721. IPD Sharing: Not stated. Countries: 2. Publications: 0.
A Study of JNJ-73763989, JNJ-64300535, and Nucleos(t)Ide Analogs in Virologically Suppressed, Hepatitis B e Antigen (HBeAg)- Negative Participants With Chronic Hepatitis B Virus Infection
ClinicalTrials.gov study NCT05123599. IPD Sharing: YES. Countries: 8. Publications: 0.
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International Brain Laboratory public data
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OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.